Educational Actions to Raise Student Awareness About the Donation and Transplantation of Human Organs and Tissues.

BACKGROUND: In Brazil, family refusal to donate organs and tissues is the main reason for not performing transplants, making it necessary to implement different educational strategies in diverse populations on this topic. Thus, this study aimed to raise awareness among school-aged adolescents about the method of organ and tissue donation and transplantation. METHODS: This is a descriptive experience report with a quantitative and qualitative approach to educational actions in the school environment, mediated by the action research method, carried out with 936 students aged between 14 and 18 years from public schools in the interior of Sao Paulo, Brazil. These actions were developed according to the themes identified and worked on through the culture circle, using active methodologies. Two semistructured questionnaires were applied, pre- and postinterventions. For analysis, sample normality tests and Student's t test were used (P < .0001). RESULTS: The topics identified were as follows: clarifications on the legislative history of donation and transplants; diagnosis of brain and circulatory death; bioethical aspects of transplants; reflections on mourning, death, and dying; maintenance and notification of the potential donor; types of viable organs and tissues for donation; and the process from collection to transplantation, among others. Comparative analysis showed statistical differences between pre- and postinterventions. CONCLUSIONS: Educational interventions through active methodologies are presented as tools capable of making students aware of organ and tissue donation and transplantation.

Temperature Monitoring of Two Different Thermal Boxes for the Transport of Biological Samples.

BACKGROUND: According to the Resolutions of the Collegiate Board of Directors RDC No. 20/2014, 214/2018, and 707/2022, validation of the temperature of thermal boxes for the transport of biological samples must be based on standardized procedures and tested by the Tissue Banks, guaranteeing safety and quality. Therefore, they can be simulated. Our objective was to monitor and compare the temperature of 2 different coolers while transporting biological samples. METHODS: The following items were packed in each of the 2 different thermal boxes (Box 1: Easy Path; Box 2: Safe Box Polyurethane Vegetal): 6 blood samples (30 mL), one bone tissue sample (200 g), 8 hard ice (Gelox, to keep the temperature <8ºC), and internal and external traceability "Time Stamp" sensors installed for measuring and storing temperature data in real-time. The monitored boxes were placed in the trunk of a bus that traveled an approximate distance of 630 km and were then placed in the trunk of a car, under direct sunlight, until they reached a temperature of 8ºC. RESULTS: In Box 1, the internal temperature was maintained in the range of -7ºC to 8ºC for approximately 26 hours. In Box 2, the internal temperature was maintained in the range of -10ºC to 8ºC for approximately 98 hours and 40 minutes. CONCLUSIONS: We concluded that both coolers, under similar storage conditions, are suitable for transporting biological samples, with Box 2 maintaining the desired temperature for longer.

Models of Quality Management Systems Applied in Specialized Services for the Donation and Transplantation of Human Organs and Tissues.

BACKGROUND: Quality management tools and models are used to improve processes and products, including those related to organ and tissue donation and transplantation protocols. This study aims to map, discuss, and disseminate models/tools of quality management systems applied in health services, specializing in the donation and/or transplantation of human organs and tissues. METHODS: This is an integrative literature review over the last 10 years, operationalized through searches carried out on the PubMed portal and the databases: SciVerse Scopus (SCOPUS), Scielo, Latin American and Caribbean Literature on Health Sciences (LILACS), Nursing Database (BDENF), and the Virtual Health Library (BVS). The organization of search results in the databases and the election of articles compatible with the guiding question and the inclusion and exclusion criteria for this study took place through the online and free application platform Rayyan. RESULTS: Six hundred seventy-eight records were found, of which, after careful analysis, 18 articles were considered relevant to the theme. We identified 17 quality management models and/or tools that emphasize using scientifically proven and/or validated techniques to reduce or eliminate the possibility of risks in the processes that permeate the stages of donation and transplantation of organs and tissues. CONCLUSION: This review brought the possible tools used and published, which can be perceived, reproduced, and improved, through the role of the multidisciplinary team in specialized centers for the donation and transplantation of human organs and tissues, aiming to offer a process of continuous improvement management to deliver better products and services.

The Significance of the Nucleic Acid Test (NAT) to Prevent Viral Contamination in Musculoskeletal Tissue Transplantation.

Objective The present study aims to highlight the significance of the nucleic acid test (NAT) for musculoskeletal tissue donation and to compare the sensitivity of this test on the different available platforms. Method The present study is a retrospective survey in a human tissue bank database and an integrative literature review encompassing the last 10 years. The PubMed portal and the SCOPUS, CINAHL, and Web of Science databases were queried for articles. Results We found no specific studies on the use and sensitivity of NAT in braindead tissue donors. The information presented in the present study consists of specific contents intended for the Brazilian Blood Transfusion Network (Hemorrede Transfusional Nacional, in Portuguese) and internal retrospective data from a tissue bank located at a city in the state of São Paulo, Brazil. Conclusions The NAT is effective in blood samples from living patients. However, since biochemical reactions in braindead patients can be different, specific research, platforms, or both are crucial to tissue banks.

The Significance of the Nucleic Acid Test (NAT) to Prevent Viral Contamination in Musculoskeletal Tissue Transplantation / A importância da realização do teste de ácidos nucleicos (NAT) para a prevenção de contaminação viral nos transplantes de tecidos musculoesqueléticos

Abstract Objective The present study aims to highlight the significance of the nucleic acid test (NAT) for musculoskeletal tissue donation and to compare the sensitivity of this test on the different available platforms. Method The present study is a retrospective survey in a human tissue bank database and an integrative literature review encompassing the last 10 years. The PubMed portal and the SCOPUS, CINAHL, and Web of Science databases were queried for articles. Results We found no specific studies on the use and sensitivity of NAT in braindead tissue donors. The information presented in the present study consists of specific contents intended for the Brazilian Blood Transfusion Network (Hemorrede Transfusional Nacional, in Portuguese) and internal retrospective data from a tissue bank located at a city in the state of São Paulo, Brazil. Conclusions The NAT is effective in blood samples from living patients. However, since biochemical reactions in braindead patients can be different, specific research, platforms, or both are crucial to tissue banks.

Resumo Objetivo Evidenciar a importância da realização do teste de ácido nucleico (NAT, na sigla em inglês) para doação de tecidos musculoesqueléticos, assim como comparar a sensibilidade deste exame nas diferentes plataformas existentes no mercado. Método Trata-se de um levantamento retrospectivo no banco de dados de um determinado Banco de Tecidos Humanos e de uma revisão integrativa da literatura, operacionalizada nos últimos 10 anos. As buscas de artigos ocorreram no portal PubMed e nas bases de dados SCOPUS, CINAHL e Web of Science. Resultados Não foram encontrados estudos específicos sobre a utilização e a sensibilidade do exame NAT em pacientes doadores de tecidos com morte encefálica (ME), sendo as informações apresentadas no presente estudo conteúdos específicos destinados à Hemorrede Transfusional Nacional e aos dados retrospectivos internos de um Banco de Tecidos do interior do estado de São Paulo, Brasil. Conclusões O exame NAT se apresenta efetivo em amostras de sangue de pacientes vivos. Porém, reações bioquímicas em pacientes com condições de ME podem se apresentar de formas diferenciadas, tornando-se indispensáveis a realização de pesquisas específicas e/ou a indicação de plataformas aos Bancos de Tecidos.

Vigilância em saúde do trabalhador: o suicídio relacionado ao trabalho / Worker's health surveillance: work-related suicide / Vigilancia de la salud del trabajador: suicidio relacionado con el trabajo

OBJETIVO: investigar as possíveis relações que permeiam as temáticas da ideação suicida e do suicídio associadas aos aspectos internos e externos do trabalho. MÉTODO: ao adotar uma abordagem qualitativa e perspectiva descritiva, foi realizada uma revisão integrativa da literatura do período de 2009-2019 operacionalizada por buscas nas bases de dados PubMed, SCOPUS, Web of Science, LILACS, BDENF e BVS. RESULTADOS: partiu-se da questão norteadora "Quais são as relações existentes entre o trabalhador, vítima de suicídio, e o ambiente de trabalho em que ele está inserido?" e de descritores, encontrando-se 481 registros, dos quais dez estudos foram selecionados. Separados por dois eixos de análise (aspectos internos e externos), os estudos descreveram dez relações predominantes entre o suicídio e o trabalho, sendo elas: depressão (19,3%); assédio moral/bullying (16,1%); ausência de lazer (12,9%); estresse (9,6%); sobrecarga profissional (9,6%); acidentes no trabalho (9,6%); Síndrome de Burnout (6,4%); isolamento social (6,4%); conflitos entre a família e o trabalho (6,4%) e falta de autonomia no trabalho (3,2%). CONCLUSÃO: os resultados demonstram a relação existente entre o trabalho e o suicídio. Como a Enfermagem é uma potencial promotora no cuidado humanizado, destaca-se a necessidade de vigilância e promoção da saúde para os trabalhadores nesses ambientes.

OBJECTIVE: to investigate the possible links between the themes of suicide ideation and suicide associated with the internal and external aspects of the work. METHOD: by adopting a qualitative approach and descriptive perspective, an integrative review of the literature of the 2009-2019 period was carried out by searching the PubMed, SCOPUS, Web of Science, LILACS, BDENF and VHL databases. RESULTS: The guiding question "What are the relations between the worker, victim of suicide, and the work environment in which he is inserted?" and descriptors, with 481 records, of which ten studies, were selected. Separated by two axes of analysis (internal and external aspects), the studies described ten predominant relationships between suicide and work, being: depression (19.3%); moral harassment/bullying (16.1%); absence of leisure (12.9%); stress (9.6%); professional overload (9.6%); accidents at work (9.6%); Burnout syndrome (6.4%); social isolation (6.4%); conflicts between family and work (6.4%) and lack of autonomy at work (3.2%). CONCLUSION: the results show the relationship between work and suicide. As nursing is a potential promoter of humanized care, the need for vigilance and health promotion for workers in these environments is highlighted.

OBJETIVO: investigar las posibles relaciones que impregnan los temas de ideación suicida y suicidio, asociados con aspectos internos y externos del trabajo. MÉTODO: adoptando un enfoque cualitativo y una perspectiva descriptiva, se realizó una revisión integradora de la literatura para el período 2009-2019 mediante la búsqueda en las bases de datos PubMed, SCOPUS, Web of Science, LILACS, BDENF y BVS. RESULTADOS: Basado en la pregunta guía: "¿Cuáles son las relaciones que existen entre el trabajador, una víctima de suicidio y el ambiente de trabajo en el que está insertado?" y descriptores, se encontraron 481 registros, de los cuales se seleccionaron 10 estudios. Separados por dos ejes de análisis (aspectos internos y externos), los estudios describieron 10 relaciones predominantes entre el suicidio y el trabajo, a saber: depresión (19.3%), bullying / bullying (16.1%), ausencia de ocio (12.9%), estrés (9.6%), sobrecarga profesional (9.6%), accidentes laborales (9.6%), síndrome de Burnout (6.4%), aislamiento social (6, 4%), conflictos entre familia y trabajo (6,4%) y falta de autonomía en el trabajo (3,2%). CONCLUSIÓN: los resultados demuestran una relación entre el trabajo y el suicidio. Como la enfermería es un potencial promotor de la atención humanizada, se destaca la necesidad de vigilancia y promoción de la salud de los trabajadores en estos entornos.

Implementation and certification of ISO 9001:2015 seal in human tissue bank HCFMRP-USP.

The Quality Management System has been a management technology applied to guarantee the quality of processes and products of a given organization. Thus, ISO 9001:2015 certification assists organizations that want to develop, implement, maintain, and improve a quality management system to enable process improvements and assessments to meet the needs of its customers. This experience report addresses the implementation and certification of the ISO 9001:2015 quality management seal at the Human Tissue Bank of the Ribeirão Preto Clinical Hospital (HCRP) at the University of São Paulo (USP) at Ribeirão Preto Medical School (FMRP)-Brazil. In 2018, the Human Tissue Bank, in partnership with HCRP, received consultancies from PRIMEMODE. The consulting consisted of visits that elucidated the processes to the employees of the Tissue Bank, enabling them to clarify the applicability of all items of the standard. In March 2019, the Tissue Bank received the audit visit and was certified with the ISO 9001:2015 Seal by the Carlos Alberto Vanzolini Foundation. The Tissue Bank aims to provide human tissues for transplantation and research from organ donors. It has a complex physical structure within the required health and legal standards. Also, now it has a quality management seal. The certification guarantees the process organization and the high quality standard of the supplied tissues.