RESUMO
BACKGROUND AND PURPOSE: Dietary fibre comprises a complex group of polysaccharides that are indigestible but are fermented by gut microbiota, promoting beneficial effects to the intestinal mucosa indirectly through the production of short chain fatty acids. We found that a polysaccharide, rhamnogalacturonan (RGal), from the plant Acmella oleracea, has direct effects on intestinal epithelial barrier function. Our objective was to determine the mechanism whereby RGal enhances epithelial barrier function. EXPERIMENTAL APPROACH: Monolayers of colonic epithelial cell lines (Caco-2, T84) and of human primary cells from organoids were mounted in Ussing chambers to assess barrier function. The cellular mechanism of RGal effects on barrier function was determined using inhibitors of TLR-4 and PKC isoforms. KEY RESULTS: Apically applied RGal (1000 µg ml-1 ) significantly enhanced barrier function as shown by increased transepithelial electrical resistance (TER) and reduced fluorescein isothiocyanate (FITC)-dextran flux in Caco-2, T84 and human primary cell monolayers, and accelerated tight junction reassembly in Caco-2 cells in a calcium switch assay. RGal also reversed the barrier-damaging effects of inflammatory cytokines on FITC-dextran flux and preserved the tight junction distribution of occludin. RGal activated TLR4 in TLR4-expressing HEK reporter cells, an effect that was inhibited by the TLR4 inhibitor, C34. The effect of RGal was also dependent on PKC, specifically the isoforms PKCδ and PKCζ. CONCLUSION AND IMPLICATIONS: RGal enhances intestinal epithelial barrier function through activation of TLR4 and PKC signalling pathways. Elucidation of RGal mechanisms of action could lead to new, dietary approaches to enhance mucosal healing in inflammatory bowel diseases.
Assuntos
Mucosa Intestinal , Ramnogalacturonanos , Receptor 4 Toll-Like , Células CACO-2 , Fibras na Dieta/farmacologia , Células Epiteliais/metabolismo , Humanos , Mucosa Intestinal/metabolismo , Microbiota , Permeabilidade , Ramnogalacturonanos/farmacologia , Junções Íntimas/metabolismo , Receptor 4 Toll-Like/metabolismoRESUMO
Polysaccharides from P. eryngii mushroom were selectively extracted using low-cost technologies (water at different conditions of temperature and pressure). Mannogalactan was the main polysaccharide in cold-water extracted fraction (CWEF), while a linear (1â6)-ß-d-glucan was the main polymer in hot-water extracted fraction (HWEF). Autoclave-extracted fraction (AEF) contained a mixture of at least four different α- and ß-glucans. The report of linear (1â6)-ß-glucan and linear (1â3)-ß-glucan is a new finding for P. eryngii fruiting bodies. The immunostimulatory properties of the fractions on THP-1 macrophages were studied. All fractions at 50, 250 and 500 µg/mL were not cytotoxic and produced different stimulus on NO, IL-1ß and IL-10 secretion by the cells. Thus, our results showed that it is possible to concentrate different P. eryngii polysaccharides in selected fractions using a simple and low-cost procedure. Since biological effects depends on the polysaccharide structure, this technique allows the obtainment of fractions with distinct immunomodulatory activities.
Assuntos
Carpóforos/química , Polissacarídeos Fúngicos/farmacologia , Fatores Imunológicos , Macrófagos/efeitos dos fármacos , Pleurotus/química , Polissacarídeos , beta-Glucanas , Misturas Complexas/química , Misturas Complexas/isolamento & purificação , Misturas Complexas/farmacologia , Polissacarídeos Fúngicos/isolamento & purificação , Humanos , Fatores Imunológicos/isolamento & purificação , Fatores Imunológicos/farmacologia , Imunomodulação , Estrutura Molecular , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Relação Estrutura-Atividade , Células THP-1 , beta-Glucanas/isolamento & purificação , beta-Glucanas/farmacologiaRESUMO
World fisheries and aquaculture production totaled 167â¯millionâ¯tons in 2014. This high fish production generates a lot of waste that could be used as raw material for extraction of substances of pharmacological interest. In this work, we extract and characterize glycosaminoglycans (GAGs) present in the viscera of Nile tilapia (Oreochromis niloticus) and Pacu (Piaractus mesopotamicus), which are among the most vastly produced fishes in inland aquaculture in Brazil. Moreover, the anticoagulant activity of the GAGs fractions was evaluated. GAGs were obtained from total defatted viscera, after proteolysis, precipitation with ethanol, anion exchange chromatography and treatment with chondroitinase. Chondroitin sulfate (CS), dermatan sulfate (DS) and heparan sulfate (HS) were identified by agarose gel electrophoresis and NMR analyses. CS, DS and HS were identified in equivalent fractions obtained from both fishes, and all GAGs fractions showed anticoagulant activity.
Assuntos
Anticoagulantes/isolamento & purificação , Anticoagulantes/farmacologia , Caraciformes/anatomia & histologia , Ciclídeos/anatomia & histologia , Glicosaminoglicanos/isolamento & purificação , Glicosaminoglicanos/farmacologia , Vísceras/química , AnimaisRESUMO
Several bioactive sulfated galactans have been isolated from the tunic of different species of ascidians. The biological activity of this kind of polysaccharides has been related with the presence and position of sulfate groups, and by the chemical composition of this kind of polysaccharides. A sulfated galactan (1000RS) was isolated from the tunic of the Brazilian ascidia Microcosmus exasperatus through proteolytic digestion, ethanol precipitation, dialysis and freeze-thaw cycles. Homogeneity and molecular weight were estimated by using size exclusion chromatography. Monosaccharide composition and type of linkage were assessed by Gas chromatography coupled to mass spectrometry and the sulfate content was quantified through gelatin/BaCl2 method. These experiments along with NMR and FTIR analysis allowed to claim that the galactan backbone is mainly composed of 4-linked α-l-Galp units. In addition, they permitted to establish that some of the galactose residues are sulfated at the 3-position. This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
Assuntos
Coagulação Sanguínea/efeitos dos fármacos , Galactanos/química , Galactanos/farmacologia , Sulfatos/química , Urocordados/química , Animais , Relação Dose-Resposta a Droga , Humanos , MetilaçãoRESUMO
Croton cajucara Benth. is a medicinal plant popularly used in the Brazilian Amazonia, where it is known as sacaca, being consumed as tea, decoction or infusion of the leaves and stem bark. From a decoction of the leaves, a comprehensive phytochemical analysis was developed by liquid chromatography-mass spectrometry. Many compounds were identified for the first time in C. cajucara, such as O-glycosides of kaempferol and quercetin, flavonoid-C-glycosides, tannins and cinnamic acid derivatives. These compounds were fractionated by polarity and assayed for their anti-inflammatory activity, using a model of mice edema, induced by an intraplantar injection of carrageenan. All fractions exhibited anti-inflammatory properties.
Assuntos
Croton , Animais , Anti-Inflamatórios , Brasil , Camundongos , Compostos Fitoquímicos , Extratos Vegetais , Plantas MedicinaisRESUMO
Citrus pectin (CP14) from Citrus sinensis, and a fucogalactan (E) and a glucan (G16) from Agaricus bisporus were isolated and structurally characterized. CP14 was constituted by (1â4)-linked α-d-GalpA units, E was composed by a (1â6)-linked α-d-Galp main-chain, partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp, and partially methylated at O-3, whereas G16 was composed of (1â6)-linked ß-d-Glcp units. The polysaccharides were sulfated giving rise to CP14S, ESL and G16S. The APTT and PT assays showed a decreasing order of anticoagulant activity for ESL, CP14S and G16S, respectively. ESL and CP14S showed greater anticoagulant activity. However, ESL reduced thrombus formation to 32.3% at a dose of 6.0mgkg-1, whereas CP14S inhibited totally the thrombus formation at 3.0mgkg-1, in vivo. NMR and methylation analyses showed that α-d-GalAp units of CP14S were sulfated in 2,3-O-position, whereas ESL was mainly sulfated in 2-O-, 2,3-O- and 2,3,4-O-positions.
RESUMO
The polysaccharide compositions of primary and secondary cell walls of members of the family Arecaceae in the commelinid clade of monocotyledonous plants have previously been found to be distinguishable from other commelinid families, and to be more similar to those of non-commelinids. However, few studies have been conducted. We aimed to extract and characterize the main cell-wall polysaccharides in the fruit pulp of tucumã (Astrocaryum aculeatum), a member of Arecaceae family. Hemicellulosic polysaccharides extracted by alkali from the fruit pulp were present in greater proportions (6.4% yield) than water-extracted ones (3.0% yield). Thus, the former was analyzed using monosaccharide composition, methylation, molecular weight determination and 13C-NMR data. The tucumã alkaline extract presented a highly ramified acidic galactoarabinoxylan (53.7%), a linear (1 â 5)-linked α-L-arabinan (27.8%), a low branched glucuronoxylan (14.1%) and small portions of a xyloglucan (4.4%). The major polysaccharide found in A. aculeatum (acidic galactoarabinoxylan) is similar to those found in other commelinid plants such as grasses and cereals.
Assuntos
Arecaceae/metabolismo , Parede Celular/metabolismo , Frutas/metabolismo , Glucanos/metabolismo , Polissacarídeos/metabolismo , Xilanos/metabolismo , Álcalis/química , Arecaceae/química , Parede Celular/química , Frutas/química , Glucanos/análise , Polissacarídeos/análise , Solubilidade , Xilanos/análiseRESUMO
The chemical features of xylan largely determine its physical and biological properties and its use in the industry. In this work, we describe the occurrence, purification and partial characterization of a xylan in edible açaí berries (Euterpe oleraceae), using a fairly simple and inexpensive method of purification from alkaline açaí extract. A mainly linear (1â4)-ß-d-xylan was found as the majority (70%) of alkali extract and 4.2% of the dry matter açaí pulp. This represents the biggest source of xylan found so far in a fruit pulp and could be suitable for applications in the industry and biomedical field.
Assuntos
Euterpe/química , Frutas/química , Xilanos/análise , Manipulação de Alimentos , Extratos Vegetais/isolamento & purificaçãoRESUMO
Electroactive hydrogels were prepared using commercial citric pectin, either raw (PC) or purified through dialysis (dPC), and chemically synthesized polypyrrole (PPy). 1H NMR analyses showed that PC is a low methoxyl pectin (degree of methoxylation, DM=46%) and dPC is a high methoxyl pectin (DM=77%). The pyrrole polymerization was monitored through UV-vis spectroscopy and both samples were observed to be good stabilizers for PPy in aqueous medium. The dispersions were used to prepare the hydrogels h-PC-PPy and h-dPC-PPy. The hydrogel h-dPC-PPy has a higher swelling index (SI≈25%) at pH 1.2 than the hydrogel h-PC-PPy (SI≈7%). Contrastingly, at pH 6.8 both hydrogels lost their mechanical integrity. Raman spectroscopy revealed that PPy is more oxidized in h-PC-PPy. Nevertheless, both hydrogels are electroactive and therefore can be considered for applications in which the control of the degree of swelling is desired.
RESUMO
Croton cajucara Benth. is a tree from the Amazon Forest, where it is known as sacaca. Its leaves and barks are used in medicinal preparations to treat different diseases, including gastric ulcers. The crude polysaccharide fraction (CCP), obtained from the hot aqueous extract of C. cajucara leaves, was able to promote gastroprotection on an ethanol induced gastric ulcer model. Therefore, a bioguided fractionation was performed to isolate the active polysaccharide fraction. After freezing-thawing, ultrafiltration and dialyses at 100, 50, and 25kDa cut-off membranes, fraction 25R was obtained. It contained glucose, galactose, rhamnose, arabinose, galacturonic acid and mannose in a 7:5:5:3:1:1 molar ratio approximately, and had a Mw of 42,840g/mol. Methylation analysis and NMR spectroscopy indicated that 25R is a very complex polysaccharide fraction containing type I rhamnogalacturonan, arabinan, type I arabinogalactan, type II arabinogalactan, rhamnan, starch and mannan. It was able to reduce ethanol-induced gastric ulcers in rats, through preservation of mucus and GSH levels.
Assuntos
Croton/química , Citoproteção/efeitos dos fármacos , Folhas de Planta/química , Polissacarídeos/química , Polissacarídeos/farmacologia , Estômago/efeitos dos fármacos , Estômago/patologia , Animais , Antiulcerosos/química , Antiulcerosos/farmacologia , Antiulcerosos/uso terapêutico , Etanol/farmacologia , Feminino , Mucosa Gástrica/metabolismo , Polissacarídeos/uso terapêutico , Ratos , Ratos Wistar , Úlcera Gástrica/induzido quimicamente , Úlcera Gástrica/tratamento farmacológico , Úlcera Gástrica/metabolismoRESUMO
Low molecular weight heparin, which is generally obtained by chemical and enzymatic depolymerization of unfractionated heparin, has high bioavailability and can be subcutaneously injected. The aim of the present investigation was to fractionate bovine heparin using a physical method (ultrafiltration through a 10kDa cut-off membrane), avoiding structural modifications that can be caused by chemical or enzymatic treatments. Two fractions with different molecular weights were obtained: the first had an intermediate molecular weight (B-IMWH; Mn=9587Da) and the other had a high molecular weight (B-HMWH; 22,396Da). B-IMWH and B-HMWH have anticoagulant activity of 103 and 154IU/mg respectively, which could be inhibited by protamine. Both fractions inhibited α-thrombin and factor Xa in vitro and showed antithrombotic effect in vivo. Moreover, ex vivo aPTT assay demonstrated that B-IMWH is absorbed by subcutaneous route. The results showed that ultrafiltration can be used to obtain two bovine heparin fractions, which differ on their molecular weights, structural components, anticoagulant potency, and administration routes.
Assuntos
Heparina de Baixo Peso Molecular/química , Heparina/química , Animais , Anticoagulantes , Bovinos , Peso Molecular , Tempo de Tromboplastina ParcialRESUMO
Açaí berries (Euterpe oleracea) are greatly consumed in Brazil and exported to other countries as a nutritional supplement, due to health benefits attributed to its consumption. However, the complete chemical structure of bioactive polysaccharides was not fully elucidated yet. In this work, we characterize pectic polysaccharides from açaí berries through monosaccharide composition, HPSEC, methylation and 13C and 1H/13C HSQC-DEPT-NMR analyses. A highly methoxylated homogalacturonan with a DM of 88% and Mw of 22kDa together with small amounts of a mannoglucan were found. Moreover, a type II arabinogalactan (Mw=45kDa) containing a backbone with high portions of 6-O-linked and 3,6-O-linked Galp chains rather than 3-O-linked Galp was also isolated and structurally characterized. The type II arabinogalactan was found as a side chain of a type I rhamnogalacturonan. These findings contribute to correlate the fine chemical structure with the previously reported action of açaí polysaccharides on innate immune response. Moreover, from the taxonomic point of view, the results bring new information about polysaccharide composition of primary cell walls of palms (Arecaceae), that despite being commelinid monocots, have a distinct cell wall composition.
Assuntos
Parede Celular/química , Euterpe/química , Frutas/química , Pectinas/isolamento & purificaçãoRESUMO
The plant Arctium lappa L. is popularly used to relieve symptoms of inflammatory disorders. A crude polysaccharide fraction (SAA) resulting of aqueous extraction of A. lappa leaves showed a dose dependent anti-edematogenic activity on carrageenan-induced paw edema, which persisted for up to 48h. Sequential fractionation by ultrafiltration at 50kDa and 30kDa cut-off membranes yielded three fractions, namely RF50, RF30, and EF30. All these maintained the anti-edematogenic effect, but RF30 showed a more potent action, inhibiting 57% of the paw edema at a dose of 4.9mg/kg. The polysaccharide RF30 contained galacturonic acid, galactose, arabinose, rhamnose, glucose, and mannose in a 7:4:2:1:2:1 ratio and had a Mw of 91,000g/mol. Methylation analysis and NMR spectroscopy indicated that RF30 is mainly constituted by a type I rhamnogalacturonan branched by side chains of types I and II arabinogalactans, and arabinan.
Assuntos
Arctium/química , Polissacarídeos/química , Polissacarídeos/farmacologia , Animais , Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , Fracionamento Químico , Cromatografia em Gel , Edema/tratamento farmacológico , Edema/patologia , Feminino , Camundongos , Monossacarídeos/análise , Polissacarídeos/uso terapêutico , Espectroscopia de Prótons por Ressonância MagnéticaRESUMO
A fucogalactan (E) was isolated from aqueous extract of Agaricus bisporus. The monosaccharide composition, methylation, and NMR analyses showed it is constituted by a (1â6)-linked α-d-Galp main-chain, partially methylated at O-3, and partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp. HPSEC analysis showed it had Mw of 1.28×10(4)gmol(-1). The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; µLmg(-1)). The degree of substitution (DS) was evaluated for all sulfated derivatives. The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays. This sulfated fucogalactan, named E100, was obtained with the optimal conditions of ηClSO3H/OH ratio of 18, VT/w ratio of 100, in 6h of reaction. The results showed that E100 produces a linear increment of APTT for concentrations of 15-45µgmL(-1), whereas PT was almost constant between 20 and 400µgmL(-1), suggesting an anticoagulant activity via inhibition of the intrinsic pathway of blood coagulation. NMR and methylation analyses showed that α-d-Galp units of the main chain were greatly sulfated on 2-O-, 3-O-, and 4-O-positions.
Assuntos
Agaricus/química , Galactanos/química , Galactanos/farmacologia , Anticoagulantes/química , Anticoagulantes/farmacologia , Coagulação Sanguínea/efeitos dos fármacos , Galactanos/isolamento & purificação , Humanos , Tempo de Tromboplastina ParcialRESUMO
Disorders of hemostasis can produce innumerous problems. Polysaccharides have been studied both as anticoagulant and as procoagulant agents. A mannan with a main chain of α-(1 â 6)-linked-Manp units, branched at O-2 mainly by side-chains of 2-O-linked-α-Manp units was chemically sulfated, structurally characterized by NMR and GC-MS (methylation, desulfation and methylation with trideuterated iodomethane), and tested in vitro and in vivo on blood coagulation models. Chemical analyses indicate a high degree of substitution on the sulfated polysaccharide. This polymer acted as a procoagulant agent, increasing blood coagulation in normal and hemophilic plasma, activated platelet aggregation and also decreased ex vivo aPTT. Polymers such as the sulfated mannan could be a helpful source of hemostatic agents to prevent hemorrhagic states.
Assuntos
Coagulação Sanguínea/efeitos dos fármacos , Mananas/química , Mananas/farmacologia , Sulfatos/química , Animais , Fator XII/metabolismo , Fator Xa/metabolismo , Feminino , Hemofilia A/fisiopatologia , Humanos , Hidrocarbonetos Iodados/química , Metilação , Agregação Plaquetária/efeitos dos fármacos , Ratos , Ratos Wistar , Trombina/metabolismo , Trombose Venosa/fisiopatologiaRESUMO
Primary cell wall polysaccharides from aqueous extract of buriti fruit pulp (Mauritia flexuosa, an exotic tropical palm) were isolated and characterized. After freeze-thaw and α-amylase treatments, extracted polysaccharides were purified by sequential ultrafiltration through membranes. Two homogeneous fractions were obtained, SBW-100R and SBW-30R (Mw of 126 kDa and 20 kDa, respectively). Monosaccharide composition, methylation and (13)C NMR analysis showed that fraction SBW-100R contained a (1 â 5)-linked arabinan, branched at O-3 and O-2 positions, linked to a type I rhamnogalacturonan. Low amounts of these polymers were also present in fraction SBW-30R according to (13)C NMR analysis and monosaccharide composition. However, a high methyl esterified homogalacturonan (HG) was present in higher proportions. These results reinforce previous findings present in literature data which indicate that pectic polysaccharides are found in high amounts in primary cell walls of palms, which are commelinid monocotyledons.
Assuntos
Arecaceae/química , Parede Celular/química , Frutas/química , Pectinas/química , Extratos Vegetais/química , Polissacarídeos/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Monossacarídeos/análiseRESUMO
Leaves of Arctium lappa contain several mono- and dicaffeoylquinic acids, as evaluated by liquid chromatography-mass spectrometry. In order to investigate the protection on gastric mucosa against ulcers, rats were treated with fractions from leaf extract prior to ethanol-induced ulcers. The original fraction obtained as ethanol soluble fraction from hot aqueous extract was able to protect de gastric mucosa, and this effect was retained in the ethyl acetate fraction, obtained from liquid/liquid fractionation. The main compound in this fraction was isolated and chemically characterized by nuclear magnetic resonance and mass spectrometry, assisted by isopropylidene derivatization which gave rise a mass increment of 40 units. Therefore, the underivatized compound that had m/z 515.119 [M-H](-) was shifted to m/z 555.151, being confirmed as 1,3-O-dicaffeoylquinic acid, which presented an ED50 of 57 µg kg(-1) on gastric protection, lesser than the therapeutic concentration of omeprazole (40 mg kg(-1)).
Assuntos
Antiulcerosos , Arctium , Ácido Quínico/análogos & derivados , Úlcera Gástrica/tratamento farmacológico , Animais , Antiulcerosos/química , Antiulcerosos/isolamento & purificação , Antiulcerosos/uso terapêutico , Etanol , Isomerismo , Extratos Vegetais/química , Folhas de Planta , Ácido Quínico/química , Ácido Quínico/isolamento & purificação , Ácido Quínico/uso terapêutico , Ratos Wistar , Úlcera Gástrica/induzido quimicamenteRESUMO
Edible mushrooms are high nutritional value foods, which contain proteins, fibers, minerals, vitamins, and carbohydrates. Among their carbohydrates are some polysaccharides with recognized therapeutic effects. It was reported in this manuscript the structural characterization and antinociceptive and anti-inflammatory activities of an exopolysaccharide (EPS) produced by Pleurotus sajor-caju. The purified EPS was a mannogalactan (PEIsR), which was composed by mannose (37.0%), galactose (39.7%), and 3-O-methyl-galactose (23.3%). The polysaccharide was purified by freeze-thawing and dialysis, and it was characterized by GC-MS analysis and NMR spectroscopy. The mannogalactan is constituted by a main chain of (1 â 6)-linked α-D-Galp and 3-O-methyl-α-D-Galp units. Some of the α-D-Galp units were substituted at O-2 by non-reducing end units of ß-D-Manp. According to the literature review conducted, this is the first time that a methylated polysaccharide was observed on EPS of P. sajor-caju. The mannogalactan was able to reduce the nociception, in vivo, in the writhing and formalin tests and also reduced the carrageenan-induced paw edema, which indicates that it could be an effective antinociceptive and anti-inflammatory agent.
Assuntos
Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Pleurotus/química , Polissacarídeos/química , Polissacarídeos/farmacologia , Analgésicos/farmacologia , Animais , Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , Cromatografia em Gel , Galactanos/química , Masculino , Camundongos , Espectroscopia de Prótons por Ressonância MagnéticaRESUMO
Glucans comprise an important class of polysaccharides present in basidiomycetes with potential biological activities. A (1 â 3)-ß-D-glucan was isolated from Pleurotus sajor-caju via extraction with hot water followed by fractionation by freeze-thawing and finally by dimethyl sulfoxide extraction. The purified polysaccharide showed a (13)C-NMR spectrum with six signals consisting of a linear glucan with a ß-anomeric signal at 102.8 ppm and a signal at 86.1 ppm relative to O-3 substitution. The other signals at 76.2, 72.9, 68.3, and 60.8 ppm were attributed to C5, C2, C4, and C6, respectively. This structure was confirmed by methylation analysis, and HSQC studies. The ß-d-glucan from P. sajor-caju presented an immunomodulatory activity on THP-1 macrophages, inhibited the inflammatory phase of nociception induced by formalin in mice, and reduced the number of total leukocytes and myeloperoxidase levels induced by LPS. Taken together, these results demonstrate that this ß-d-glucan exhibits a significant anti-inflammatory activity.
Assuntos
Anti-Inflamatórios/química , Inflamação/induzido quimicamente , Polissacarídeos/química , beta-Glucanas/química , Animais , Anti-Inflamatórios/administração & dosagem , Dimetil Sulfóxido/química , Formaldeído/toxicidade , Imunomodulação/efeitos dos fármacos , Inflamação/tratamento farmacológico , Macrófagos/efeitos dos fármacos , Camundongos , Nociceptividade/efeitos dos fármacos , Pleurotus/química , Polissacarídeos/administração & dosagem , Proteoglicanas , beta-Glucanas/administração & dosagemRESUMO
Prunes are the dried fruits from Prunus domestica. After the purification steps, two homogeneous polysaccharides were characterised, SF-50R and SF-50E and contained Ara:Gal:Rha:GalA in 47.8:31.5:10.7:10.0 and 39.6:50.3:5.1:5.0 molar ratios, respectively. Methylation analysis and (13)C NMR spectroscopy indicated that both fractions are constituted by rhamnogalacturonans with type I arabinogalactans as side chains, differing mainly in the proportions of the rhamnogalacturonan backbone, in the length of the (1â4)-ß-galactan chain and in the proportion of the arabinan side chain. Crude water extract (PWH) and fraction SF-50E were evaluated for their gastroprotective properties against ethanol-induced acute gastric lesions in rats. Oral administration of PWH (3 and 10mg/kg) reduced the gastric lesion area by 67±11% and 60±12%, respectively, while fraction SF-50E (10 and 30mg/kg) inhibited the lesion area by 84±12% and 83±12%, respectively. These results indicated that prune's polysaccharides act as gastroprotective agents in rats.