Assuntos
Carcinoma de Células Escamosas/cirurgia , Neoplasias Labiais/cirurgia , Lábio/cirurgia , Retalho Miocutâneo , Procedimentos de Cirurgia Plástica/métodos , Idoso , Bochecha , Músculos Faciais/cirurgia , Humanos , Complicações Intraoperatórias/prevenção & controle , Masculino , Pessoa de Meia-IdadeAssuntos
Ameloblastoma/cirurgia , Neoplasias Mandibulares/cirurgia , Osteotomia Mandibular/métodos , Reconstrução Mandibular/métodos , Osteogênese por Distração/instrumentação , Adulto , Ameloblastoma/diagnóstico por imagem , Desenho de Equipamento , Humanos , Imageamento Tridimensional , Masculino , Neoplasias Mandibulares/diagnóstico por imagem , Osteogênese por Distração/métodos , Radiografia Panorâmica , Tomografia Computadorizada por Raios XRESUMO
Several advances have been made toward understanding the biology of cancer and most of them are due to robust genetic studies that led to the scientific recognition that although many patients have the same type of cancer their tumors may have harbored different molecular alterations. Personalized therapy and the development of advanced techniques of preclinical imaging and new murine models of disease are emerging concepts that are allowing mapping of disease markers in vivo and in some cases also receptor targeted therapy. Aim of this review is to illustrate some emerging models of disease that allow patient tumor implantation in mice for subsequent drug testing and advanced approaches for therapy mediated by preclinical imaging. In particular we discuss targeted therapy mediated by high frequency ultrasound and magnetic resonance, two emerging techniques in molecular preclinical therapy.
Assuntos
Diagnóstico por Imagem/métodos , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Animais , Sistemas de Liberação de Medicamentos , Humanos , Medicina de PrecisãoRESUMO
Intratumoral injections of a replication-incompetent adenovirus (Ad) expressing melanoma differentiation-associated gene-7/interleukin-24 (Ad.mda-7), a secreted cytokine displaying cancer-selective, apoptosis-inducing properties, profoundly inhibits prostate cancer (PC) growth in immune-incompetent animals. In contrast, Ad.mda-7 is ineffective in PCs overexpressing antiapoptotic proteins such as Bcl-2 or Bcl-x(L). However, intratumoral injections of a conditionally replication-competent Ad (CRCA) in which expression of the adenoviral E1A gene is driven by the cancer-specific promoter of progression-elevated gene-3 (PEG-3) and which simultaneously expresses mda-7/interleukin (IL)-24 in the E3 region of the Ad (Ad.PEG-E1A-mda-7), a cancer terminator virus (CTV), is highly active in these cells. A major challenge for gene therapy is systemic delivery of nucleic acids directly into an affected tissue. Ultrasound (US) contrast agents (microbubbles-MBs) are viable candidates for gene delivery/therapy. Here, we show that MB/Ad.mda-7 complexes targeted to DU-145 cells using US dramatically reduced tumor burden in xenografted nude mice. Additionally, US-guided MB/CTV delivery completely eradicated not only targeted DU-145/Bcl-x(L)-therapy-resistant tumors, but also nontargeted distant tumors (established in the opposite flank), thereby implementing a cure. These findings highlight potential therapeutic applications of this novel image-guided gene therapy technology for advanced PC patients with metastatic disease.