Biological and metabolomics-guided isolation of tetrahydrofurofuran lignan from Croton spp. with antiproliferative activity against human melanoma cell line.

The Croton genus (Euphorbiaceae) is recognized as a promising source for identifying bioactive compounds with antiproliferative activity. However, knowledge on the chemical composition and activity of Croton floribundus, Croton echinocarpus, and Croton zehntneri is limited. Thus, this study aimed to investigate the antiproliferative activity of these species on cells derived from tumoral breast, lung, and melanoma cells, and primary fibroblasts derived from human skin. Metabolomic strategies were applied via ultra-performance liquid chromatography coupled with high-resolution mass spectrometry and multivariate statistical analysis to target the main active compound. The C. floribundus leaf extract exhibited the highest activity, with an IC50 value lower than that of the reference drug - temozolomide - in the most responsive cell line - SK-MEL-147 - and in all the evaluated melanoma cell lines (SK-MEL-147, CHL-1 and WM-1366). Four tetrahydrofurofuran lignans were isolated for the first time from the most promising fraction of the C. floribundus extract. According to the metabolomic and multivariate statistical analyses, the isolated lignan epi-yangambin constituted the main antiproliferative compound against SK-MEL-147; furthermore, it exhibited selective antiproliferative activity for this cell line (IC50 = 13.09 µg/mL and selectivity index = 3.82; temozolomide, IC50 = 121.50 µg/mL) due to, at least in part, its ability to inhibit cell cycle progression at G2/M. This is especially relevant considering the high resistance of melanoma cells to available drugs. Thus, epi-yangambin can serve as a prototype for further antiproliferative investigations.

Antiproliferative Potential of Essential Oil from the Leaves of Croton floribundus (Euphorbiaceae) Against Human Metastatic Melanoma Cell Lines.

Melanoma is a cancer type with high lethality, metastatic capacity, and limited therapeutic options. Different essential oils have been reported with antitumoral potential. Thus, the essential oil (EO) of the leaves of C. floribundus was obtained by hydrodistillation and analyzed by GC-MS. The majority of substances annotated were ß-selinene, E-Caryophyllene, and Premnaspirodiene. The cytotoxic activity of EO was evaluated on three melanoma cell lines SKMEL-147, WM-1366, and CHL-1, which are representative of metastatic melanoma with different mutation profiles. The IC50 values found for EO were lower than temozolomide (reference drug) in all melanoma cell lines. In addition, the selectivity of EO was upward when compared to the reference drug. Interestingly, the WM-1366 cell line was the most responsive, and these findings are very promising considering that it has shown high resistance to the plethora of compounds. Thus, the C. floribundus EO is a promising source to drive further studies for the development of new treatments for metastatic melanoma, which is urgently relevant given the resistance of this pathology to current treatments.

Manganese reductive dissolution coupled to Sb mobilization in contaminated shooting range soil.

A "redox-stat" RMnR bioreactor was employed to simulate moderately reducing conditions (+ 420 mV) in Sb-contaminated shooting range soils for approximately 3 months, thermodynamically favoring Mn(IV) reduction. The impact of moderately reducing conditions on elemental mobilization (Mn, Sb, Fe) and speciation [Sb(III) versus Sb(V); Fe2+/Fe3+] was compared to a control bioreactor RCTRL without a fixed redox potential. In both bioreactors, reducing conditions were accompanied by an increase in effluent Sb(V) and Mn(II) concentrations, suggesting that Sb(V) was released through microbial reduction of Mn oxyhydroxide minerals. This was underlined by multiple linear regression analysis showing a significant (p < 0.05) relationship between Mn and Sb effluent concentrations. Mn concentration was the sole variable exhibiting a statistically significant effect on Sb in RMnR, while under the more reducing conditions in RCTRL, pH and redox potential were also significant. Analysis of the bacterial community composition revealed an increase in the genera Azoarcus, Flavisolibacter, Luteimonas, and Mesorhizobium concerning the initial soil, some of which are possible key players in the process of Sb mobilization. The overall amount of Sb released in the RMnR (10.40%) was virtually the same as in the RCTRL (10.37%), which underlines a subordinate role of anoxic processes, such as Fe-reductive dissolution, in Sb mobilization. This research underscores the central role of relatively low concentrations of Mn oxyhydroxides in influencing the fate of trace elements. Our study also demonstrates that bioreactors operated as redox-stats represent versatile tools that allow quantifying the contribution of specific mechanisms determining the fate of trace elements in contaminated soils. KEY POINTS: • "Redox-stat" reactors elucidate Sb mobilization mechanisms • Mn oxyhydroxides microbial reductive dissolution has a major role in Sb mobilization in soils under moderately reducing conditions • Despite aging the soil exhibited significant Sb mobilization potential, emphasizing persistent environmental effects.

Relevance of sarcopenia in elderly patients undergoing surgery for oral squamous cell carcinoma.

In the elderly population there is increasing evidence that frailty predicts adverse outcomes better than chronological age. Sarcopenia is an important component of frailty. This study aimed to establish the relevance of sarcopenia in elderly patients with oral squamous cell carcinoma (OSCC) undergoing surgery. This retrospective, single-centre, cohort study included patients over the age of 75 years who were diagnosed with OSCC between 2007 and 2016. Cross-sectional imaging of the neck was used to predict the Skeletal Muscle Index (SMI) using validated equations. Based on established thresholds, patients were categorised as having either a normal or low SMI, indicative of sarcopenia. Sixty-nine patients met the inclusion criteria. Patients with a low SMI had a longer length of stay (16.9 days vs 9.8 days, p = 0.030); they had more severe complications, defined as Clavien-Dindo grade IIIb or higher (17.6% vs 4.0%, p = 0.042); and their mean Comprehensive Complication Index (CCI) was also higher (14.1 vs 4.7, p = 0.051). Furthermore, 2/34 patients in the low SMI group died within 30 days of surgery compared with none in the normal SMI group (5.9% vs 0%, p = 0.503). Whilst patients with a low SMI who underwent surgery had lower five-year overall survival, the difference was not statistically significant. This study shows that sarcopenia negatively influences surgical outcomes in elderly patients. Routine measurement of SMI could be an indication for a comprehensive geriatric assessment (CGA).

Labor gymnastics in health professionals: a systematic review / Gimnasia laboral para profesionales de la salud: una revisión sistemática / Ginástica laboral em profissionais de saúde: uma revisão sistemática

ABSTRACT This aimed to systematically review randomized controlled trials and compare the effectiveness of labor gymnastics with that of no intervention, minimal intervention or other types of intervention in healthcare workers, in relation to musculoskeletal pain, stress, physical disability, and absence from work. A search was carried out in the PUBMED, Pedro, EMBASE, CENTRAL, CINAHAL, PSYCHINFO, NIOSHTIC-2, SPORT DICUS, SCIELO, and LILACS databases. In total, 3,598 articles were found, seven of which were eligible for the study. There was a statistical difference in musculoskeletal pain in favor of labor gymnastics after 5, 10 and 12 weeks (MD: −0.63; 95%, CI: −1.17; −0.08) and 6, 9 and 12 months of intervention (MD: −0.74; 95% CI: −1.43; −0.05). There was also a statistical difference in favor of labor gymnastics in terms of time off work (MD: −3.26; 95% CI: −6.28; −0.25) and stress (SMD: −0.35; 95% CI: −0.67; −0.03) in studies in which interventions were carried out for 5 and 10 weeks. Labor gymnastics can contribute to the physical and mental health of healthcare professionals. However, more randomized controlled studies with a larger sample size and aimed at this professional category are needed.

RESUMEN Este estudio tuvo por objetivo realizar una revisión sistemática de los ensayos aleatorizados controlados y comparar la efectividad de la gimnasia laboral con ninguna intervención, con intervención mínima u otros tipos de intervención en los profesionales de la salud con relación a dolor musculoesquelético, estrés, incapacidad física y baja laboral. Se realizaron búsquedas en las bases de datos PUBMED, PEDro, EMBASE, CENTRAL, CINAHAL, PsycINFO, NIOSHTIC-2, SPORTDicus, SciELO y LILACS. Se encontraron 3.598 artículos, de los cuales siete fueron elegibles. Hubo una diferencia estadística para el dolor musculoesquelético a favor de la gimnasia laboral después de 5, 10 y 12 semanas (MD: −0,63; 95% CI: −1,17; −0,08) y 6,9 y 12 meses de intervención (MD: −0,74; 95% CI: −1,43; −0,05). También hubo una diferencia estadística a favor de la gimnasia laboral para el tiempo de baja laboral (MD: −3,26; 95% IC: −6,28; −0,25) y la reducción del estrés (SMD: −0,35; 95% IC: −0,67; −0,03) en los estudios que realizaron la intervención entre cinco y diez semanas. La gimnasia laboral puede contribuir a la salud física y mental de los profesionales de la salud, sin embargo, son necesarios más estudios aleatorizados controlados dirigidos a esta categoría profesional y con un mayor tamaño muestral para confirmar esta hipótesis.

RESUMO Este estudo tem como objetivo revisar sistematicamente ensaios randomizados controlados e comparar a eficácia da ginástica laboral com nenhuma intervenção, intervenção mínima ou outros tipos de intervenção em trabalhadores de saúde, em relação à dor musculoesquelética, estresse, incapacidade física e afastamento do trabalho. Foram realizadas buscas nas bases de dados PUBMED, PEDro, EMBASE, CENTRAL, CINAHAL, PsycINFO, NIOSHTIC-2, SPORTDicus, SCIELO e LILACS. Foram encontrados 3598 artigos, sendo sete elegíveis. Houve diferença estatística para dor musculoesquelética a favor da ginástica laboral após 5, 10 e 12 semanas (MD: −0,63; 95%, CI: −1,17; −0,08) e 6,9 e 12 meses de intervenção (MD: −0,74; 95% CI: −1,43; −0,05). Também foi verificada diferença estatística a favor da ginástica laboral para o afastamento no trabalho (MD: −3,26; 95% IC: −6,28; −0,25) e para redução do estresse (SMD: −0.35; 95% IC: −0,67; −0.03) nos estudos que realizaram intervenção por 5 e 10 semanas. A ginástica laboral pode contribuir para a saúde física e mental do profissional de saúde, no entanto, mais estudos randomizados controlados voltados para essa categoria profissional, e com maior valor amostral, são necessários para confirmação dessa hipótese.

The Top 100 Most-Cited Articles on Permanent Teeth Avulsion: A Bibliometric Analysis.

INTRODUCTION: Knowledge of bibliometric indices on the avulsion of permanent teeth can help researchers to identify gaps in the literature. The aim of this study was to analyze the bibliometric characteristics of the 100 most-cited articles on the avulsion of permanent teeth. MATERIALS AND METHODS: Two researchers conducted an unfiltered search in the Core Collection section of the Web of Science (WoS-CC) database. The following data were extracted: position in the citation list, total number and density of citations, title, authors, institution, country, year of publication, journal, keywords, study design, tooth storage medium, and other topics of interest. Bibliometric networks were generated and analyzed using the VOSviewer software. Descriptive analysis and a Poisson regression analysis were performed (P < .05). RESULTS: The number of citations per article varied between 31 and 407. The average was 70.05, and 13 articles received more than 100 citations. J.O. Andreasen (n = 13) and M. Trope (n = 15) were the authors who contributed most often to publications. The United States was the country with the highest number of articles (n = 31). Authors associated with the University of Copenhagen, Denmark (n = 9) and University of North Carolina, USA (n = 10) showed higher productivity. In vitro/animal model studies were the most frequent study designs (n = 50), and the most-discussed topic of interest was prognosis (n = 47). There was strong clustering among 13 research groups. CONCLUSION: Observational studies, published by authors from Europe and about prognosis or treatment presented more citation ratio in WoS-CC. Clinical studies on permanent tooth avulsion are needed to increase the quality of scientific evidence and provide insights for health professionals and public policy makers.

Increased Heat Pain Tolerance but Hyperalgesia to Tonic Inflammatory Pain in the CRND8 Mouse Model of Alzheimer's Disease.

BACKGROUND: The effects of Alzheimer's disease (AD) pathology on the experience of pain are poorly understood. OBJECTIVE: To understand the pathophysiological mechanisms underlying pain sensory transmission in the transgenic mouse model of AD, CRND8. METHODS: We explored AD-related pathology in the spinal cord and dorsal root ganglia of 18-week-old female CRND8 mice. We assessed nociceptive responses to both acute heat stimuli and persistent inflammatory pain in CRND8 mice and non-transgenic (non-Tg) littermates. In addition, we searched for differences in biochemical correlates of inflammatory pain between CRND8 and non-Tg mice. Finally, we investigated the excitability of dorsal horn noc iceptive neurons in spinal cord slices from CRND8 and non-Tg mice. RESULTS: We demonstrated the presence of intracellular AD-like pathology in the spinal cord and in the dorsal root ganglia nociceptive sensory neurons of CRND8 mice. We found that CRND8 mice had a reduced susceptibility to acute noxious heat stimuli and an increased sensitivity to tonic inflammatory pain. Tonic inflammatory pain correlated with a lack of induction of pro-opiomelanocortin in the spinal cord of CRND8 mice as compared to non-Tg mice. Electrophysiological recording in acute spinal cord slice preparations indicated an increased probability of glutamate release at the membrane of dorsal horn nociceptive neurons in CRND8 mice. CONCLUSION: This study suggests that an increased thermal tolerance and a facilitation of nociception by peripheral inflammation can coexist in AD.

Prevalência de feminicídios na Grande Vitória envolvendo região craniofacial no período de pandemia do vírus Sars-CoV-2 / Feminicide prevalence in Grande Vitória involving the craniofacial region during the pandemic of the Sars-CoV-2 virus

Introdução: feminicídio é o homicídio que envolve violência doméstica e familiar, menosprezo ou discriminação ao fato da vítima ser mulher. Em 2020, foi decretada pandemia do vírus SARS-CoV-2 e como forma de reduzir a disseminação foi adotado o isolamento social, expondo muitas mulheres a um maior risco de violência doméstica. Estudos apontam que a maioria dos traumatismos relacionados à violência contra mulher ocorrem na face, ressaltando a importância de estudar os casos de feminicídio envolvendo a região bucomaxilofacial. Objetivo: verificar a prevalência de feminicídio, envolvendo lesões em região craniofacial, no período anterior (2018 e 2019) e durante (2020 e 2021) a pandemia da coronavírus. Métodos: foi realizada análise descritiva, por meio da frequência numérica e percentual do número de feminicídios envolvendo a região craniofacial no período de 2018 a 2021. Resultados: o número de laudos analisados de feminicídio ocorridos na Grande Vitória foi 47, sendo 24 antes e 23 durante a pandemia. Averiguou-se também que em 68,83% dos casos houve a presença de lesão craniofacial, principalmente em região do crânio e extraoral. Conclusão: não ocorreu variação significativa na prevalência de feminicídio antes e durante a pandemia do vírus SARS-CoV-2 na região da Grande Vitória, no Estado do Espírito Santo

Introduction: femicide is homicide that involves domestic and family violence, contempt or discrimination against the fact that the victim is a woman. In 2020, pandemic of SARS-CoV-2 virus was declared and, to reduce the spread of the disease, social isolation was adopted, exposing many women to a greater risk of domestic violence. Studies indicate that most traumas occur on the face, highlighting the importance of studying cases of femicide involving the oral and maxillofacial region. Purpose: to verify the prevalence of femicide, involving lesions in the craniofacial region, in the period before (2018 and 2019) and during (2020 and 2021) the coronavirus pandemic Methods: a descriptive analysis was carried out, through the numerical frequency and percentage of the number of femicides involving the craniofacial region in the period from 2018 to 2021. Results: the number of reports of femicide analyzed in Vitória was 47, with 24 before and 23 during the pandemic, with no significant variation. It was also found that in 68.83% of the total cases there was the presence of craniofacial lesion, mainly in the skull and extraoral region. Conclusion: there was no significant variation in the prevalence of femicide before and during the SARS-CoV-2 virus pandemic in the Grande Vitória region, in the state of Espírito Santo

Association between genetic factors and molar-incisor hypomineralisation or hypomineralised second primary molar: A systematic review.

OBJECTIVE: To determine the association between genetic factors and molar-incisor hypomineralisation (MIH) and/or hypomineralised second primary molars by means of a systematic review. DESIGN: A search was performed in Medline-PubMed, Scopus, Embase and Web of Science databases; manual search and search in gray literature were also performed. Selection of articles was performed independently by two researchers. A third examiner was involved in cases of disagreement. Data extraction was performed using an Excel® spreadsheet and independent analysis was performed for each outcome. RESULTS: Sixteen studies were included. There was an association between MIH and genetic variants related to amelogenesis, immune response, xenobiotic detoxification and other genes. Moreover, interactions between amelogenesis and immune response genes, and SNPs in the aquaporin gene and vitamin D receptors were associated with MIH. Greater agreement of MIH was found in pairs of monozygotic twins than dizygotic twins. The heritability of MIH was 20 %. Hypomineralised second primary molars was associated with SNPs in the hypoxia-related HIF-1 gene and methylation in genes related to amelogenesis. CONCLUSION: With very low or low certainty of evidence, an association was observed between MIH and SNPs in genes associated with amelogenesis, immune response, xenobiotic detox and ion transport. Interactions between genes related to amelogenesis and immune response as well as aquaporin genes were associated to MIH. With very low certainty of evidence, hypomineralised second primary molars was associated to a hypoxia-related gene and to methylation in genes related to amelogenesis. Moreover, higher agreement of MIH in pairs of monozygotic twins than dizygotic twins was observed.

Tecnologias para a adesão ao tratamento da tuberculose: um protocolo de scoping review / Technologies for adherence to tuberculosis treatment: a scoping review protocol

OBJETIVO: Identificar e mapear as tecnologias desenvolvidas e/ou validadas para incentivar e auxiliar na adesão ao tratamento da tuberculose na Atenção Primária à Saúde. MÉTODO: Trata-se de uma scoping review, seguindo as orientações do Joanna Briggs Institute (JBI) Reviewer's Manual, com a seguinte pergunta de pesquisa: "Quais as tecnologias têm sido desenvolvidas e/ou validadas para incentivar e auxiliar na adesão ao tratamento da tuberculose na Atenção Primária à Saúde?" Serão incluídos artigos, teses e dissertações a partir de buscas nas bases: Medical Literature Analysis and Retrieval System Online (Medline/PubMed), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS)/Biblioteca Virtual de Saúde (BVS), Cumulative Index to Nursing and Allied Health (CINAHL), Web of Science (WOS), Scopus, Excerpta Medica Database (Embase) e literatura cinzenta. A busca, avaliação, seleção e extração dos dados serão realizadas de forma cega entre pares; quando houver divergências, um terceiro revisor será consultado. Os resultados serão descritos na íntegra, utilizando-se de narrativa e diagramas, de forma que estejam alinhados com o objetivo e a questão norteadora desta revisão. Protocolo registrado na Open Science Framework (OSF): 10.17605/OSF.IO/PKWTC.

OBJECTIVE: To identify and map technologies developed and/or validated to promote and support adherence to tuberculosis treatment in primary health care settings. METHOD: This is a scoping review, following the guidelines of the Joanna Briggs Institute (JBI) Reviewer's Manual, with the following research question: "What technologies have been developed and/or validated to promote and support adherence to tuberculosis treatment in primary health care?". Articles, theses, and dissertations will be included by searching the following databases Medical Literature Analysis and Retrieval System Online (Medline/PubMed), Latin American and Caribbean Health Sciences Literature (LILACS)/Virtual Health Library (VHL), Cumulative Index to Nursing and Allied Health (CINAHL), Web of Science (WOS), Scopus, Excerpta Medica Database (Embase), and gray literature. The search, assessment, selection, and data extraction will be performed in a blinded manner between peers; in case of disagreement, a third reviewer will be consulted. The results will be fully described using narratives and graphs to align with the objective and guiding question of this review. Protocol registered with the Open Science Framework (OSF): 10.17605/OSF.IO/PKWTC.

Molecular identification of the emerging Human Gemykibivirus-2 (HuGkV-2) among Brazilian blood donors.

Human gemykibivirus-2 (HuGkV-2) belonging to the Gemykibivirus genus (Genomoviridae family) is an emerging DNA virus which has been described as a component of the virome of a wide variety of samples including clinical ones. So far, the HuGkV-2 DNA prevalence in the human population as well as its clinical impact are completely unknown. The objective of this study was to investigate the HuGkV-2 DNA prevalence among Brazilian healthy blood donors from three different geographic regions. A total of 450 blood samples were screened for HuGkV-2 DNA (150 samples were from the Brazilian Amazon, 150 from Midwest Brazil and 150 from South Brazil). The overall HuGkV-2 DNA prevalence was 7.8 %. Considering the examined regions, the highest prevalence was observed in the Brazilian Amazon (city of Macapa, state of Amapa), 15.3 %, followed by the Midwest Brazil (city of Brasilia, Federal District) (6.0 %) and South Brazil (city of Santa Maria, Rio Grande do Sul State) (2.0 %). This study gives preliminary insights on the molecular prevalence of HuGkV-2 DNA among Brazilian blood donors, highlighting that the highest HuGkV-2 prevalence was recorded in the Brazilian Amazon. However, more studies regarding the prevalence, transmission routes and any possible clinical effects appear to be crucial in order to understand the impact of this emerging viral agent.

Perfil epidemiológico dos casos de hanseníase em um estado do nordeste brasileiro de 2018 a 2022 / Epidemiological profile of the cases of leprosy in a state in the northeast of brazil from 2018 to 2022 / Perfil epidemiológico de los casos de hanseniasis en un estado del noreste de brasil de 2018 a 2022

A hanseníase é uma doença crônica, infectocontagiosa, de notificação compulsória e investigação obrigatória no Brasil, de evolução lenta, causada pelo bacilo Mycobacterium leprae que acomete predominantemente pele e nervos periféricos causando incapacidades funcionais permanentes de diversos graus, além de ser uma patologia ainda cercada por estigma e discriminação social. O objetivo do artigo consiste em avaliar o perfil epidemiológico da hanseníase no Estado do Rio Grande do Norte entre 2018 a 2022, estado que ocupa, atualmente, o 3° lugar a nível nacional de registro geral de casos de hanseníase. Trata-se de um estudo epidemiológico, descritivo, retrospectivo e de abordagem quantitativa, com base em dados obtidos no Departamento de Informática do Sistema Único de Saúde (DATASUS) e analisados com auxílio do software livre Jamovi 2.3.21 solid. O perfil epidemiológico da hanseníase no período de 2018 a 2022, no Estado do Rio Grande do Norte, apresentou predomínio pelo sexo feminino, acometendo adultos entre 50 a 69 anos de idade, com registro ignorado ou em branco no grau de escolaridade, predominando a forma multibacilar do tipo Virchowiana, causando grau 0 de incapacidade e a grande parte dos casos evoluindo para a cura. Portanto, faz-se relevante conhecer melhor o perfil epidemiológico e a realidade de cada município, visto que é uma patologia fortemente relacionada a condições econômicas, sociais e ambientais desfavoráveis passíveis de intervenção.

Leprosy is a chronic, contagious infectious disease, compulsorily notifiable and compulsory research in Brazil, of slow evolution, caused by the bacillus Mycobacterium leprae that predominantly affects skin and peripheral nerves causing permanent functional impairments of various degrees, besides being a pathology still surrounded by stigma and social discrimination. The objective of the article is to evaluate the epidemiological profile of leprosy in the State of Rio Grande do Norte between 2018 and 2022, which currently occupies the 3rd place at the national level of general registration of cases of leprosy. This is an epidemiological, descriptive, retrospective and quantitative approach study, based on data obtained in the Department of Information Technology of the Unified Health System (DATASUS) and analyzed with the help of free software Jamovi 2.3.21 solid. The epidemiological profile of leprosy in the period from 2018 to 2022, in the state of Rio Grande do Norte, showed predominance by the female sex, affecting adults between 50 and 69 years of age, with ignored or blank registration in the level of schooling, predominating the multibacillary form of the Virchowiana type, causing grade 0 disability and the great majority of cases evolving to cure. Therefore, it is important to know better the epidemiological profile and reality of each municipality, since it is a pathology strongly related to unfavorable economic, social and environmental conditions that can intervene.

La Hanseniasis es una enfermedad crónica, una enfermedad contagiosa, una notificación obligatoria e investigación obligatoria en Brasil, de evolución lenta, causada por el bacilo Mycobacterium leprae, que ataca predominantemente la piel y los nervios periféricos, causando discapacidades funcionales permanentes de diversos grados, además de ser una enfermedad aún rodeada de estigma y discriminación social. El propósito del artículo es evaluar el perfil epidemiológico de la lepra en el estado de Río Grande del Norte entre 2018 y 2022, un estado que actualmente ocupa el tercer lugar en el registro general nacional de casos de lepra. Se trata de un estudio de enfoque epidemiológico, descriptivo, retrospectivo y cuantitativo basado en datos obtenidos del Departamento de Datos del Sistema de Salud Pública (DATASUS) y analizado con la ayuda del software sólido Jamovi 2.3.21 libre. El perfil epidemiológico de la lepra en el período 2018-2022, en el estado de Rio Grande do Norte, mostró predominio del sexo femenino, atacando a adultos de 50 a 69 años, con un registro desconocido o en blanco en el grado de escolaridad, predominando en la forma multibacible del tipo Virchowiana, causando un grado de discapacidad y el gran número de casos. evolucionando hacia la curación. Por lo tanto, es importante conocer mejor el perfil epidemiológico y la realidad de cada municipio, ya que se trata de una enfermedad que está fuertemente relacionada con condiciones económicas, sociales y ambientales desfavorables que pueden ser intervenidas.

Molecular identification of the emerging human Gemykibivirus-2 (HuGkV-2) among Brazilian blood donors

Human gemykibivirus-2 (HuGkV-2) belonging to the Gemykibivirus genus (Genomoviridae family) is an emerging DNA virus which has been described as a component of the virome of a wide variety of samples including clinical ones. So far, the HuGkV-2 DNA prevalence in the human population as well as its clinical impact are completely unknown. The objective of this study was to investigate the HuGkV-2 DNA prevalence among Brazilian healthy blood donors from three different geographic regions. A total of 450 blood samples were screened for HuGkV-2 DNA (150 samples were from the Brazilian Amazon, 150 from Midwest Brazil and 150 from South Brazil). The overall HuGkV-2 DNA prevalence was 7.8 %. Considering the examined regions, the highest prevalence was observed in the Brazilian Amazon (city of Macapa, state of Amapa), 15.3 %, followed by the Midwest Brazil (city of Brasilia, Federal District) (6.0 %) and South Brazil (city of Santa Maria, Rio Grande do Sul State) (2.0 %). This study gives preliminary insights on the molecular prevalence of HuGkV-2 DNA among Brazilian blood donors, highlighting that the highest HuGkV-2 prevalence was recorded in the Brazilian Amazon. However, more studies regarding the prevalence, transmission routes and any possible clinical effects appear to be crucial in order to understand the impact of this emerging viral agent.

Methanolic Extracts of Chiococca alba in Aedes aegypti Biorational Management: Larvicidal and Repellent Potential, and Selectivity against Non-Target Organisms.

The use of formulations containing botanical products for controlling insects that vector human and animal diseases has increased in recent years. Plant extracts seem to offer fewer risks to the environment and to human health without reducing the application strategy's efficacy when compared to synthetic and conventional insecticides and repellents. Here, we evaluated the potential of extracts obtained from caninana, Chiococca alba (L.) Hitchc. (Rubiaceae), plants as a tool to be integrated into the management of Aedes aegypti, one of the principal vectors for the transmission of arborviruses in humans. We assessed the larvicidal and repellence performance against adult mosquitoes and evaluated the potential undesired effects of the extracts on non-target organisms. We assessed the susceptibility and predatory abilities of the nymphs of Belostoma anurum, a naturally occurring mosquito larva predator, and evaluated the C. alba extract's cytotoxic effects in mammalian cell lines. Our chromatographic analysis revealed 18 compounds, including rutin, naringin, myricetin, morin, and quercetin. The methanolic extracts of C. alba showed larvicidal (LC50 = 82 (72-94) mg/mL) activity without killing or affecting the abilities of B. anurum to prey upon mosquito larvae. Our in silico predictions revealed the molecular interactions between rutin and the AeagOBP1 receptor to be one possible mechanism for the repellent potential recorded for formulations containing C. alba extracts. Low cytotoxicity against mammalian cell lines reinforces the selectivity of C. alba extracts. Collectively, our findings highlight the potential of C. alba and one of its constituents (rutin) as alternative tools to be integrated into the management of A. aegypti mosquitoes.

The role of brain oscillations in post-stroke motor recovery: An overview.

Stroke is the second cause of disability and death worldwide, highly impacting patient's quality of life. Several changes in brain architecture and function led by stroke can be disclosed by neurophysiological techniques. Specifically, electroencephalogram (EEG) can disclose brain oscillatory rhythms, which can be considered as a possible outcome measure for stroke recovery, and potentially shaped by neuromodulation techniques. We performed a review of randomized controlled trials on the role of brain oscillations in patients with post-stroke searching the following databases: Pubmed, Scopus, and the Web of Science, from 2012 to 2022. Thirteen studies involving 346 patients in total were included. Patients in the control groups received various treatments (sham or different stimulation modalities) in different post-stroke phases. This review describes the state of the art in the existing randomized controlled trials evaluating post-stroke motor function recovery after conventional rehabilitation treatment associated with neuromodulation techniques. Moreover, the role of brain pattern rhythms to modulate cortical excitability has been analyzed. To date, neuromodulation approaches could be considered a valid tool to improve stroke rehabilitation outcomes, despite more high-quality, and homogeneous randomized clinical trials are needed to determine to which extent motor functional impairment after stroke can be improved by neuromodulation approaches and which one could provide better functional outcomes. However, the high reproducibility of brain oscillatory rhythms could be considered a promising predictive outcome measure applicable to evaluate patients with stroke recovery after rehabilitation.

Emergent Neuroimaging Findings for Written Expression in Children: A Scoping Review.

BACKGROUND: There is currently a dearth of research on the neural framework of writing tasks in children, as measured by neuroimaging techniques. OBJECTIVE: This paper provides an overview of the current literature examining the neurological underpinnings of written expression in children. DESIGN: Using a scoping review approach, with thorough searches of key databases, this paper presents the available literature comprising 13 different studies using both structural and functional neuroimaging techniques with the 0-18 English speaking population. RESULTS: Studies largely presented small sample sizes, with most studies utilizing elementary or middle school-aged children. Emergent findings revealed a complex network of neural contributions to the writing process in children. There were associations between the left fusiform gyrus and orthographic coding (i.e., handwriting), and spelling and written composition measures were significantly correlated with activity in the left posterior cingulate, left precuneus, and right precuneus regions. Additionally, results revealed that good versus poor writers manifested differential brain activation patterns during many tasks associated with written expression, with good writers performing more efficiently than poor writers with respect to brain regions activated during a writing task across handwriting, spelling, and idea generation. CONCLUSIONS: The findings from this scoping review lay the foundation for future studies examining the interface between writing skills in children and underlying neural pathways that support the various components of the writing process. It will be important for future research to examine the neurological bases of the various components of written expression in children and adolescents.

Conhecimento e conduta de graduandos em odontologia da UFES frente à violência infantil: um estudo transversal / Knowledge and conduct of the UFES dentistry students about child violence: a cross-sectional study

Introdução: o cirurgião-dentista possui grande relevância na identificação de abuso e negligência infantil, pois a região craniofacial é frequentemente acometida. Objetivo: verificar o conhecimento e a conduta dos estudantes de Odontologia sobre violência infantil, bem como a aquisição de conhecimentos durante a formação universitária. Métodos: Estudo transversal com 190 alunos matriculados do terceiro ao décimo período de odontologia de uma universidade pública. A coleta dos dados foi realizada por meio de um questionário aplicado em sala de aula contendo questões sobre a violência infantil e a conduta do cirurgião-dentista. Para a análise de dados foi realizada estatística descritiva, por meio da frequência numérica e percentual. Resultados: Os estudantes acertaram 60% das questões referentes à identificação das características orofaciais da violência infantil. Apenas 51,05% obtiveram informações sobre o assunto, sendo que a principal fonte relatada foi a internet. Concomitantemente, 32% não se consideraram capazes de identificar casos de maus-tratos infantis. Em relação à responsabilidade do cirurgião-dentista, 93,16% acredita que este possui o dever ético de denunciar e, também, 87,37% a obrigatoriedade legal de notificar casos suspeitos ou confirmados de violência infantil. Quanto ao local onde fazer a denúncia, o Conselho tutelar foi o mais referido (78,4%). Conclusão: No geral, os graduandos possuem conhecimentos satisfatórios sobre o assunto, tanto na parte de identificação quanto da conduta. No entanto, sugere-se que o tema seja mais especificamente abordado nos Projetos Pedagógicos dos Cursos de Odontologia

Introduction: the dentist has great relevance in the identification of child abuse and neglect, as the craniofacial region is frequently affected. Purpose: to verify the knowledge and conduct of dentistry students about child violence, as well as the acquisition of knowledge during university education. Material and Methods: Cross-sectional study with 190 students enrolled from the third to the tenth period of dentistry at a public university. Data collection was performed using a questionnaire applied in the classroom containing questions about child violence and the behavior of the dentist. For data analysis, descriptive statistics were performed, by means of numerical and percentage frequency. Results: Students answered 60% of the questions regarding the identification of orofacial characteristics of child violence. Only 51.05% obtained information on the subject, and the main source reported was the internet. Concomitantly, 32% did not consider themselves capable of identifying cases of child abuse. Regarding the responsibility of the dentist, 93.16% believe that he has an ethical duty to report and, also, 87.37% has the legal obligation to notify suspected or confirmed cases of child violence. Regarding the place where to make the complaint, the tutelary Council was the most mentioned (78.4%). Conclusion: In general, undergraduates have satisfactory knowledge on the subject, both in terms of identification and conduct. However, it is suggested that the theme be more specifically addressed in the Pedagogical Projects of Dentistry Courses

Vantagens e limitações em uma identificação odontológica radiográfica ­ relato de caso pericial / Advantages and limitations on a radiographic dental identification - forensic case report

A identificação de cadáveres carbonizados exige uma abordagem multidisciplinar, com finalidade de apontar características únicas de um indivíduo e possibilitar distingui-lo de todos os demais. Devido à dificuldade na inspeção visual e pelo método datiloscópico, a Odontologia Legal se torna fundamental, em virtude da singularidade relacionada aos arcos dentais. O objetivo do trabalho foi apresentar as vantagens e limitações encontradas durante a identificação odontológica radiográfica de um corpo carbonizado e a importância da odontologia legal, por meio de um relato de caso pericial, demonstrando a possibilidade de identificação do cadáver mesmo diante de entraves. O exame necroscópico odontolegal realizado em um Departamento Médico-Legal do Espírito Santo constatou que na maxila existia uma grande destruição dos dentes superiores e presença de prótese parcial removível superior, sendo que não foi possível realiza-lo na mandíbula por conta do estado de calcinação em que se encontrava. Por meio da comparação das características odontológicas entre uma radiografia panorâmica antemortem e radiografias periapicais postmortem, foi possível uma identificação positiva do cadáver carbonizado levando em consideração a quantidade e à qualidade dos achados odontológicos semelhantes encontrados nas imagens, associados à ausência de divergências inexplicáveis

Charred corpse identification requires a multidisciplinary approach, in order to identify unique characteristic and therefore make it possible to distinguish the corpse from everyone else. Due to the difficulty to use visual inspection and the fingerprint method, Forensic Dentistry becomes essential, due to the uniqueness related to dental arches. The objective of the study was to present the advantages and limitations found during the radiographic dental identification of a charred body and the importance of legal dentistry, through an expert case report, demonstrating the possibility of identifying the corpse even in the face of obstacles. The postmortem dental examination carried out in a Medical-Legal Department of Espirito Santo found that in the maxilla there was great destruction of the upper teeth and the presence of an upper removable partial prosthesis, and it was not possible to perform it in the mandible due to the state of calcination in which it was found. By comparing the dental characteristics between an ante-mortem panoramic radiograph and post-mortem periapical radiographs, it was possible to positively identify the charred cadaver taking into account the quantity and quality of similar dental findings in the images, associated with the absence of inexplicable divergences

Diagnóstico e tratamento de úlcera hipertensiva de Martorell: artigo de revisão / Diagnóstico y tratamiento de la úlcera hipertensiva de Martorell: artículo de revisión / Diagnosis and treatment of Martorell's hypertensive ulcer: review article

Introdução: A úlcera hipertensiva de Martorell é uma ferida crônica, associada a hipertensão arterial sistêmica, dedifícil manejo clínico e subdiagnosticada. Objetivo: Investigar os métodos de diagnóstico e os tratamentos existentes para a UHM. Método: Estudo descritivo e qualitativo de revisão integrativa com a análise sistemática de referências bibliográficas nas bases de dados PubMed, SciELO, MEDLINE e LILACS no período de 2016 e 2021, e periódicos da CAPES. Resultados: Clinicamente o paciente apresenta uma ferida pequena, de forma arredondada, superficial, com margens necróticas e cianóticas e eritema perilesional eventual. Os pulsos distais usualmente estão presentes e há ausência de edema e varizes locais. De todos os sinais e sintomas relatados, destaca-se a dor, descrita como intensa e desproporcional à sua dimensão. Conclusão: O cerne ao diagnóstico é avaliação clínica e o tratamento é a normalização da pressão arterial sistêmica e curativos regulares e, uso de analgésicos, antibióticos e vasodilatadores periféricos.(AU)

lntroduction: Martorell's hypertensive ulcer is a chronic wound, associated with systemic hypertension withdifficult clinicai management and it is often underdiagnosed. Goal: To investigate the diagnostic method and current treatments for Martorell's hypertensive ulcer disease. Method: A descriptive and qualitative study of integrative literature review with a systematic analysis of bibliographic references from PubMed, SciELO, MEDLINE and LILACS databases, ranging from 2016 to 2021, as well as selected articles from CAPES journals. Results: patients usually present with a small, rounded and superficial wound with cyanotic and necrotic edges and occasional perilesional erythema. Distal pulses are usually present and there is an absence of edema and local varicose veins. From all the signs and symptoms reported by patients with this type of injury, it is possible to point out that the pain is intense and disproportionate to its dimension. Conclusion: The core of diagnosis is the clinicai evaluation and the treatment is controlled systemic blood pressure, regular bandages and, if needed, analgesics, antibiotics and peripheral vasodilators. (AU)

lntrodución: La úlcera hipertensiva de Martorell es una herida crónica, asociada a la hipertensión arterial sistémica,de difícil manejo clínico y muchas veces subdiagnosticada. Objetivo: Investigar los métodos de diagnóstico y tratamientos existentes para este tipo de lesión. Método: Se realizá un análisis sistemático de referencias bibliográficas en las bases de datas PubMed, SciELO, MEDLINE y LILACS en el período de publicación entre 2016 y 2021, además de artículos seleccionados de revistas CAPES. Resultados: Clínicamente el paciente suele presentarse con una pequena herida, redondeada, superficial, con márgenes necróticos y cianóticos con eritema perilesional ocasional. Los pulsos distales suelen estar presentes y hay ausencia de edema y venas varicosas locales. De todos los signos y síntomas reportados por los pacientes con este tipo de lesiones, se destaca el dolor, que se describe como intenso y desproporcionado con la zona de la herida y su tamafio. Conclusión: La base dei tratamiento es la normalización de la presión arterial sistémica y los apósitos regulares y, si necesario, analgésicos, antibióticos y vaso dilatadores periféricos(AU)

Enfermeiro: ator no empreendedorismo social / Nurse: actor in social entrepreneurships

Introdução: O empreendedor social é um agente de mudanças que inspira outras pessoas a se engajarem em torno de uma causa comum e corrobora com a melhoria de ambientes onde os recursos são escassos. O enfermeiro realiza o empreendedorismo social, uma vez que busca soluções inovadoras e busca a sustentabilidade social. Objetivo: discutir o papel do Enfermeiro como ator no empreendorismo social. Método: Estudo descritivo e qualitativo de revisão integrativa de literatura nas bases de dados Pubmed, Lilacs, Scielo e indexador Spell, entre 2010 a 2020 sobre empreendedorismo social e enfermagem. Resultados: Foram encontradas vinte pesquisas, do qual 25% retrata as características empreendedoras de acadêmicos, docentes e enfermeiros; 25% aborda o empreendedorismo na formação dos enfermeiros; 15% dos estudos trazem informações nacionais e internacionais sobre a temática. Conclusão: É essencial discutir sobre o papel do enfermeiro enquanto empreendedor social, sua formação e atuação para seu empoderamento na assistência de saúde.(AU)

Introduction: The social entrepreneur is an agent of change that inspires others to engage around a common cause and supports the improvement of environments where resources are scarce. Nurses carry out social entrepreneurship, as they seek innovative solutions and seek social sustainability. Objective: to discuss the role of the nurse as an actor in social entrepreneurship. Method: Descriptive and qualitative study of an integrative literature review in Pubmed, Lilacs, Scielo and Spell indexer databases, between 2010 and 2020 on social entrepreneurship and nursing. Results: Twenty researches were found, of which 25% portray the entrepreneurial characteristics of academics, teachers and nurses; 25% addresses entrepreneurship in the training of nurses; 15% of the studies bring national and international information on the subject. Conclusion: It is essential to discuss the role of nurses as social entrepreneurs, their training and performance for their empowerment in health care.(AU)

El emprendedor social es un agente de cambio que inspira a otras personas a involucrarse en una causa común. El enfermero realiza emprendimiento social al buscar soluciones innovadoras y la sostenibilidad social. Objetivo: discutir el rol del enfermero como actor en el emprendimiento social. Método: Estudio descriptivo y cualitativo de revisión integradora de literatura sobre emprendimiento social y enfermería en las bases de datos Pubmed, Lilacs, Scielo y Spell index, entre 2010 y 2020. Resultados: Se encontraron veinte encuestas, de las cuales el 25% retrata las características emprendedoras de académicos, docentes y enfermeros; El 25% aborda el espíritu empresarial en la formación de enfermeros; El 15% de los estudios aportan información nacional e internacional sobre el tema. Conclusión: Discutir sobre el rol del enfermero como emprendedor social, su formación y desempeño para que estos profesionales se empoderen en el cuidado de la salud.(AU)