Automatic bright-field smear microscopy for diagnosis of pulmonary tuberculosis.

In recent decades, many studies have been published on the use of automatic smear microscopy for diagnosing pulmonary tuberculosis (TB). Most of them deal with a preliminary step of the diagnosis, the bacilli detection, whereas sputum smear microscopy for diagnosis of pulmonary TB comprises detecting and reporting the number of bacilli found in at least 100 microscopic fields, according to the 5 grading scales (negative, scanty, 1+, 2+ and 3+) endorsed by the World Health Organization (WHO). Pulmonary TB diagnosis in bright-field smear microscopy, however, depends upon the attention of a trained and motivated technician, while the automated TB diagnosis requires little or no interpretation by a technician. As far as we know, this work proposes the first automatic method for pulmonary TB diagnosis in bright-field smear microscopy, according to the WHO recommendations. The proposed method comprises a semantic segmentation step, using a deep neural network, followed by a filtering step aiming to reduce the number of false positives (false bacilli): color and shape filtering. In semantic segmentation, different configurations of encoders are evaluated, using depth-wise separable convolution layers and channel attention mechanism. The proposed method was evaluated with a large, robust, and annotated image dataset designed for this purpose, consisting of 250 testing sets, 50 sets for each of the 5 TB diagnostic classes. The following performance metrics were obtained for automatic pulmonary TB diagnosis by smear microscopy: mean precision of 0.894, mean recall of 0.896, and mean F1-score of 0.895.

Evaluating the performance of convolutional neural networks with direct acyclic graph architectures in automatic segmentation of breast lesion in US images.

BACKGROUND: Outlining lesion contours in Ultra Sound (US) breast images is an important step in breast cancer diagnosis. Malignant lesions infiltrate the surrounding tissue, generating irregular contours, with spiculation and angulated margins, whereas benign lesions produce contours with a smooth outline and elliptical shape. In breast imaging, the majority of the existing publications in the literature focus on using Convolutional Neural Networks (CNNs) for segmentation and classification of lesions in mammographic images. In this study our main objective is to assess the ability of CNNs in detecting contour irregularities in breast lesions in US images. METHODS: In this study we compare the performance of two CNNs with Direct Acyclic Graph (DAG) architecture and one CNN with a series architecture for breast lesion segmentation in US images. DAG and series architectures are both feedforward networks. The difference is that a DAG architecture could have more than one path between the first layer and end layer, whereas a series architecture has only one path from the beginning layer to the end layer. The CNN architectures were evaluated with two datasets. RESULTS: With the more complex DAG architecture, the following mean values were obtained for the metrics used to evaluate the segmented contours: global accuracy: 0.956; IOU: 0.876; F measure: 68.77%; Dice coefficient: 0.892. CONCLUSION: The CNN DAG architecture shows the best metric values used for quantitatively evaluating the segmented contours compared with the gold-standard contours. The segmented contours obtained with this architecture also have more details and irregularities, like the gold-standard contours.

A fully automatic method for recognizing hand configurations of Brazilian sign language

Abstract Introduction Sign language is a collection of gestures, postures, movements, and facial expressions used by deaf people. The Brazilian sign language is Libras. The use of Libras has been increased among the deaf communities, but is still not disseminated outside this community. Sign language recognition is a field of research, which intends to help the deaf community communication with non-hearing-impaired people. In this context, this paper describes a new method for recognizing hand configurations of Libras - using depth maps obtained with a Kinect® sensor. Methods The proposed method comprises three phases: hand segmentation, feature extraction, and classification. The segmentation phase is independent from the background and depends only on pixel value. The feature extraction process is independent from rotation and translation. The features are extracted employing two techniques: (2D)2LDA and (2D)2PCA. The classification employs two classifiers: a novelty classifier and a KNN classifier. A robust database is constructed for classifier evaluation, with 12,200 images of Libras and 200 gestures of each hand configuration. Results The best accuracy obtained was 96.31%. Conclusion The best gesture recognition accuracy obtained is much higher than the studies previously published. It must be emphasized that this recognition rate is obtained for different conditions of hand rotation and proximity of the depth camera, and with a depth camera resolution of only 640×480 pixels. This performance must be also credited to the feature extraction technique, and to the size standardization and normalization processes used previously to feature extraction step.

Breast tumor classification in ultrasound images using support vector machines and neural networks

Abstract Introduction The use of tools for computer-aided diagnosis (CAD) has been proposed for detection and classification of breast cancer. Concerning breast cancer image diagnosing with ultrasound, some results found in literature show that morphological features perform better than texture features for lesions differentiation, and indicate that a reduced set of features performs better than a larger one. Methods This study evaluated the performance of support vector machines (SVM) with different kernels combinations, and neural networks with different stop criteria, for classifying breast cancer nodules. Twenty-two morphological features from the contour of 100 BUS images were used as input for classifiers and then a scalar feature selection technique with correlation was used to reduce the features dataset. Results The best results obtained for accuracy and area under ROC curve were 96.98% and 0.980, respectively, both with neural networks using the whole set of features. Conclusion The performance obtained with neural networks with the selected stop criterion was better than the ones obtained with SVM. Whilst using neural networks the results were better with all 22 features, SVM classifiers performed better with a reduced set of 6 features.

Automatic identification of tuberculosis mycobacterium

Introduction According to the Global TB control report of 2013, “Tuberculosis (TB) remains a major global health problem. In 2012, an estimated 8.6 million people developed TB and 1.3 million died from the disease. Two main sputum smear microscopy techniques are used for TB diagnosis: Fluorescence microscopy and conventional microscopy. Fluorescence microscopy is a more expensive diagnostic method because of the high costs of the microscopy unit and its maintenance. Therefore, conventional microscopy is more appropriate for use in developing countries. Methods This paper presents a new method for detecting tuberculosis bacillus in conventional sputum smear microscopy. The method consists of two main steps, bacillus segmentation and post-processing. In the first step, the scalar selection technique was used to select input variables for the segmentation classifiers from four color spaces. Thirty features were used, including the subtractions of the color components of different color spaces. In the post-processing step, three filters were used to separate bacilli from artifact: a size filter, a geometric filter and a Rule-based filter that uses the components of the RGB color space. Results In bacillus identification, an overall sensitivity of 96.80% and an error rate of 3.38% were obtained. An image database with 120-sputum-smear microscopy slices of 12 patients with objects marked as bacillus, agglomerated bacillus and artifact was generated and is now available online. Conclusions The best results were obtained with a support vector machine in bacillus segmentation associated with the application of the three post-processing filters.

Proposing the novelty classifier for face recognition

INTRODUCTION: Face recognition, one of the most explored themes in biometry, is used in a wide range of applications: access control, forensic detection, surveillance and monitoring systems, and robotic and human machine interactions. In this paper, a new classifier is proposed for face recognition: the novelty classifier. METHODS: The performance of a novelty classifier is compared with the performance of the nearest neighbor classifier. The ORL face image database was used. Three methods were employed for characteristic extraction: principal component analysis, bi-dimensional principal component analysis with dimension reduction in one dimension and bi-dimensional principal component analysis with dimension reduction in two directions. RESULTS: In identification mode, the best recognition rate with the leave-one-out strategy is equal to 100%. In the verification mode, the best recognition rate was also 100%. For the half-half strategy, the best recognition rate in the identification mode is equal to 98.5%, and in the verification mode, 88%. CONCLUSION: For face recognition, the novelty classifier performs comparable to the best results already published in the literature, which further confirms the novelty classifier as an important pattern recognition method in biometry.