Strategies for the quality control of Chrysanthemi Flos: Rapid quantification and end-to-end fingerprint conversion based on FT-NIR spectroscopy.

INTRODUCTION: Chrysanthemi Flos (CF) is widely used as a natural medicine or tea. Due to its diverse cultivation regions, CF exhibits varying quality. Therefore, the quality and swiftness in evaluation holds paramount significance for CF. OBJECTIVE: The aim of the study was to construct a comprehensive evaluation strategy for assessing CF quality using HPLC, near-infrared (NIR) spectroscopy, and chemometrics, which included the rapid quantification analyses of chemical components and the Fourier transform (FT)-NIR to HPLC conversion of fingerprints. MATERIALS AND METHODS: A total of 145 CF samples were utilised for data collection via NIR spectroscopy and HPLC. The partial least squares regression (PLSR) models were optimised using various spectral preprocessing and variable selection methods to predict the chemical composition content in CF. Both direct standardisation (DS) and PLSR algorithms were employed to establish the fingerprint conversion model from the FT-NIR spectrum to HPLC, and the model's performance was assessed through similarity and cluster analysis. RESULTS: The optimised PLSR quantitative models can effectively predict the content of eight chemical components in CF. Both DS and PLSR algorithms achieve the calibration conversion of CF fingerprints from FT-NIR to HPLC, and the predicted and measured HPLC fingerprints are highly similar. Notably, the best model relies on CF powder FT-NIR spectra and DS algorithm [root mean square error of prediction (RMSEP) = 2.7590, R2 = 0.8558]. A high average similarity (0.9184) prevails between predicted and measured fingerprints of test set samples, and the results of the clustering analysis exhibit a high level of consistency. CONCLUSION: This comprehensive strategy provides a novel and dependable approach for the rapid quality evaluation of CF.

On-line screening of natural antioxidants and the antioxidant activity prediction for the extracts from flowers of Chrysanthemum morifolium ramat.

ETHNOPHARMACOLOGY RELEVANCE: Chrysanthemum morifolium Ramat. (Flos Chrysanthemi, FC) the most economically significant "food and drug dual-use" plants, with positive effects on relieving eye fatigue, and reduce internal heat, shows significant activities, such as anti-inflammatory, antioxidant, and neuroprotective, as well as alleviating diabetes effects. AIM OF THE STUDY: This study was undertaken to a screening of natural antioxidants in five kinds of medicinal FC and development of an integrated quality control method based on the antioxidant activity. MATERIALS AND METHODS: A novel quality control method for FC was established by combining the on-line HPLC-DPPH, ESI-MS, and NIR spectra analysis. Firstly, the on-line HPLC-DPPH-MS system was employed to identify the antioxidants in FC extracts. Then, the relationship between the NIR spectra and antioxidant activities of FC samples was calibrated to evaluate the total antioxidant capacity of FC rapidly. RESULTS: The established antioxidant activity-fingerprints contain both chemical information and antioxidant activity characteristics of FC. A total of 16 antioxidants were identified by on-line HPLC-ESI-MS analysis. The results of heat map analysis and cluster analysis showed that the classification method based on antioxidants in FC can be used to identify different cultivars of FC. The optimal pretreatment of the NIR spectra was determined to be row center (RC) 1st der + multiple-scatter correction (MSC) with an optimal LV value of 11. The developed spectral-antioxidant activity model had the excellent predictive ability and was successfully used to evaluate new batches of FC samples, where Rcal = 0.9445 and Rval = 0.8821. CONCLUSIONS: This comprehensive strategy may prove to be a powerful technique for the rapid screening, identification, and activity prediction of antioxidants, which could be used for the quality control of FC, and can serve as reference for design of quality control of other herbs and foods samples.

Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.

Periodontitis is a bacterial infectious disease leading to the loss of periodontal supporting tissues and teeth. The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate. Besides, they need extra tailoring according to variform defects before implantation, leading to imprecise match. This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR. Inspired by the gelation process of the jelly, the phase change (liquid to solid) of CTP-SA after injection could automatch variform bone defects. Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms). Moreover, the reactive oxygen species released under BL excitation could accelerate the oxidation of Cu+ to Cu2+. Afterward, osteogenesis could be enhanced through two factors simultaneously: the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation. Collectively, through this dual-light (blue and NIR) noninvasive regulation, CTP-SA could switch antibacterial and osteogenic modes to address requirements of patients at different healing stages, thereby realizing the customized GTR procedures.

Tremella-Like ZnO@Col-I-Decorated Titanium Surfaces with Dual-Light-Defined Broad-Spectrum Antibacterial and Triple Osteogenic Properties.

The growing population of peri-implant diseases (PIDs) has become a public obsession, mainly due to the lack of antibacterial ability and osteogenic promotion of titanium (Ti) implants. Herein, inspired by tremella, we reported zinc oxide (ZnO)@collagen type I (Col-I)-decorated Ti for PIDs treatments. Compared with pure Ti implants, ZnO@Col-I-decorated Ti could be activated by a safe visible yellow light and showed excellent broad-spectrum antibacterial properties. The proliferation and osteogenic gene expression of bone marrow mesenchymal stem cells (BMSCs) indicated that the triple osseointegration of implants was realized through (I) the remarkedly improved surface hydrophilicity of ZnO@Col-I-decorated Ti, (II) the function of Col-I, and (III) the excellent near-infrared (NIR)-induced photothermal performance of ZnO. Collectively, the proposed dual-light-defined ZnO@Col-I coating was a promising implant surface modification system to provide customized treatments for each PID patient.