[Final evaluation of Control Program of Key Parasitic Diseases in Jinhu County (2006-2015), Jiangsu Province].

OBJECTIVE: To evaluate the implementation of the Control Program of Key Parasitic Diseases in Jinhu County from 2006 to 2015, so as to provide the evidence for the future work of prevention and control. METHODS: The historical data about key parasitoses were collected, and the organization management, financial support, capacity building, control effects, and so on were evaluated with the descriptive method. RESULTS: From 2006 to 2015, totally 19 technique educations were conducted, 844.2 thousands RMB was invested, and 1 725 person-times of technicians were trained. Totally 197 600 person-times of drug administration were performed and 11 762 person-times of residents were tested for parasites, with the infection rates of soil-transmitted nematodes fluctuating from 0.16% to 2.18%. The infection rates of Clonorchis sinensis were from 0.00% to 0.67%, and the infection rates of its intermediate hosts were from 3.81% to 9.48%. No imago and larva of Angiostrongylus cantonensis were found in the longitudinal surveillance. In 2013, the awareness rate of health related knowledge was up to 95.62%, and the correct rate of health behaviors was up to 96.46%. Totally 3 764 villages were renovated, and the beneficial rate of tapwater was up to 98.90%. The popularity rate of household toilets was up to 95.31%, and the popularity rate of harmlessness health toilets was up to 93.45%. CONCLUSIONS: The endemic situation of key parasitoses is in a low state in Jinhu County, however, the transmitted risk still exists, and therefore, the surveillance work need to be enhanced.

[Epidemiological situation of human parasitic diseases through three investigations in Jinhu County, Jiangsu Province].

OBJECTIVE: To understand the epidemiological situation of human parasitic diseases in Jinhu County, so as to provide the evidence for formulating further control strategy. METHODS: The investigated local residents were sampled by the random cluster sampling method in 1989, 1999 and 2015. The infections of intestinal helminthes were detected by Kato-Katz technique, the eggs of Enterubius vermicularis were examined by cellophane anal swab for children, the intestinal protozoa were examined by the saline smear and iodine staining methods. RESULTS: The total rates of parasitic infections were 62.57%, 9.32% and 0.49% in 1989, 1999 and 2015, respectively. Compared to those in 1989 and in 1999, the infection rate in 2015 was decreased by 99.22% and 94.74%, respectively. The numbers of detected parasite species were 14, 10 and 4 in 1989, 1999 and 2015, respectively. The intensities of infections were mainly mild in three investigations, and the intensities of all the infections in 2015 were mild. The species of infected parasites were mainly single, however, multiple infections were observed in 1989, including 4 parasite species (0.72%) and 3 parasite species (7.02%). Only in 1989, the difference between sexes was significant and the infection rate of the female was higher than that of the male (χ2 ＝18.01, P＜0.01). CONCLUSIONS: The infection rates of human parasites are decreased gradually and stabilized at the low level in Jinhu County. However, the surveillance work still should be strengthened to consolidate the achievement.

Development of high-performance liquid chromatographic for quality and authenticity control of Chinese propolis.

A RP-high-performance liquid chromatography (HPLC) method was developed for quality control of Chinese propolis by simultaneous analysis of 12 flavonoids and 8 phenolic acids. The results showed that vanillic acid, rutin, myricetin, and luteolin were not detected in all of the analyzed propolis and poplar tree gum samples. The caffeic acid, ferulic acid and p-coumaric acid were not detected in poplar tree gum but were detected in propolis, which suggest that they are practical indexes of distinguishing propolis from poplar tree gum. The flavonoid profiles of poplar tree gum were found to be similar to those of propolis, which are dominated by pinobanksin, pinocembrin, 3-O-acetylpinobanksin, chrysin, and galangin. Therefore, the proposed method could be applied to exclude poplar tree gum from propolis with cafferic acid, ferulic acid, and p-coumaric acid as qualitative markers, and distinguish poplar source resin from other illegal substances, and evaluate the quality grading of poplar-type propolis with pinobanksin, pinocembrin, 3-O-acetylpinobanksin, chrysin, and galangin as qualitative and quantitative markers.