Lagrangian solver for coupling hydrodynamics with biokinetic conversion modelling in anaerobic digesters.

Conventional anaerobic digestion models used in wastewater treatment plants suffer from inaccuracies due to the limited consideration given to hydrodynamics within the digester tank. A solution to this is to combine computational fluid dynamics simulations with anaerobic models. This paper introduces a novel methodology in the form of a software toolbox that implements the standard anaerobic digestion model no.1 in C++ and can interface with particle-based Lagrangian simulations. This method provides significantly more insights into the biochemical conversion process by accounting for the impact of the hydrodynamics on the biochemical reactions. The paper presents the background of the method along with a conceptual and numerical verification. It also presents a case study of a 3D lab scale digester comparing the results from the solver with the standard anaerobic digestion model. This integrated approach can be used by operators and designers for optimisations and also for predictive modelling.

Integrating biokinetics with computational fluid dynamics for energy performance analysis in anaerobic digestion.

Anaerobic digestion (AD) is an effective process for decomposing organic matter in wastewater treatment plants (WWTPs) where highly efficient digesters properly mix the sludge. To ensure a uniform substance distribution, a comprehensive modeling method is necessary. Computational fluid dynamics (CFD) helps in the modeling of AD tanks but few studies have focused on integrating hydrodynamics with biokinetics because of complex AD processes. The current study presents a new CFD platform for estimating the biokinetics of WWTPs to assess the energy performance of AD tanks. The presented method is validated by numerical and experimental studies, and facilitates a link between methane production and mixing energy consumption. The on-site settings of the recirculation mixing system in the studied WWTP was able to prepare a uniform mixture of the material. However, reducing mixing rate to decrease energy consumption did not lead to proper mixing quality.

On the effect of the inlet configuration for anaerobic digester mixing.

Sludge recirculation mixing in anaerobic digesters is essential for the stable operation of the digestion process. While often neglected, the configuration of the sludge inlet has a substantial influence on the efficiency of the mixing process. The fluid is either injected directly into the enclosed fluid domain or splashes onto the free surface of the slurry flow. In this paper, the aim was to investigate the effect of the inlet configuration by means of computational fluid dynamics-using ANSYS Fluent. Single-phase and multi-phase models are applied for a submerged and splashing inlet, respectively. To reduce the high computational demand, we also develop surrogate single-phase models for the splashing inlet. The digester mixing is analyzed by comparing velocity contours, velocity profiles, mixing time and dead volume. The non-Newtonian characteristics of the sludge is considered, and a [Formula: see text] model is employed for obtaining turbulence closure. Our method is validated by means of a previous study on the same geometry. Applying a submerged inlet configuration, the resulting dead volume in the tank is estimated around 80 times lower than for the case of a splashing inlet. Additionally, by emulating the multi-phase model for splashing inlet configurations with a single-phase one, the simulation clock time reduced to 15%.

Prevalence of cytomegalovirus infection and its role in total immunoglobulin pattern in Iranian patients with different subtypes of multiple sclerosis.

Multiple sclerosis (MS) is the most common autoimmune disease characterized by multifocal areas of inflammatory demyelination within the central nervous system. Cytomegalovirus (CMV) has a complex pathobiology and in most cases is simply asymptomatic. There is some recent controversy over the role of CMV in the pathology of MS. The aim of this study was to evaluate active CMV infection and its effect on the humoral immunity in patients with MS. Serum, plasma, peripheral blood mononuclear cells (PBMCs), saliva and urine collected from MS patients (n=78) and healthy subjects (n=123) were screened for the presence of anti-CMV antibodies and CMV-DNA by nephelometric and PCR methods. Concentrations of total antibodies in MS subtypes were measured using both nephelometric and enzyme linked fluorescent assay (ELFA) techniques. The results extend the observation of an increased frequency of CMV-DNA in patients, in contrast with controls (p<0.001). Furthermore, systemic CMV infections were found in 25.5% of patients and only 3.2% of controls (p<0.001). There was significant difference in the titers of anti-CMV IgG and total IgE in patient and controls (P<0.001). These results support the hypothesis that CMV may contribute to MS thought to establish systemic infection process and induce immune response.