Influence of trophic overlaps and trophic niche amplitude on microplastic intake of fish species in shallow areas of a neotropical coastal lagoon.

Plastic pollution is a global challenge that affects all marine ecosystems, and reflects all types of uses and activities of human society in these environments. In marine ecosystems, microplastics and mesoplastics interact with invertebrates and become available to higher predators, such as fish, which can ingest these contaminants. This study aimed to analyze how ecological food interactions (diet overlap and trophic niche amplitude) among fish species contribute to the ingestion of plastic particles. The gastrointestinal contents of six fish species (Atherinella brasiliensis, Eucinostomus melanopterus, Eucinostomus argenteus, Genidens genidens, Coptodon rendalli, and Geophagus brasiliensis) were analyzed to identify prey items and plastic ingestion. Based on the ontogenetic classification, A. brasiliensis, E. melanopterus, and G. genidens were divided into juveniles and adults, and the six fish species analyzed were divided into nine predator groups. Most of the plastics ingested by the fish species were blue microplastic (MP) fibers (< 0.05 mm) classified as polyester terephthalate, polyethylene, and polybutadiene. Considering all the analyzed predators, the average number and weight of plastics ingested per individual were 2.01 and 0.0005 g, respectively. We observed that predators with a high trophic overlap could present a relationship with the intake of MP fibers owing to predation on the same resources. In addition, we observed the general pattern that when a species expands its trophic diversity and niche, it can become more susceptible to plastic ingestion. For example, the species with the highest Levin niche amplitude, E. argenteus juveniles, had the highest mean number (2.9) of ingested MP fibers. Understanding the feeding ecology and interactions among species, considering how each predator uses habitats and food resources, can provide a better understanding of how plastic particle contamination occurs and which habitats are contaminated with these polluting substances.

Composition and spatial distribution of floating plastic debris along the estuarine ecocline of a subtropical coastal lagoon in the Western Atlantic.

With the objective of characterizing the composition and spatial distribution of plastic fragments in a subtropical lagoon system, five sample areas affected by various anthropogenic impacts were chosen in the southern part of the Estuarine Lagoon System in Laguna, Santa Catarina, Brazil. The total density of the floating meso- and microplastics encountered was 7.32/m3, with the greatest density in the access channel and external area of the lagoon. Plastic filament was the most abundant and mainly comprised polyester (PET), polypropylene (PP) and polyethylene (PE) from 0.05 to 0.71 mm2. Fishing and urbanization were the main sources of the meso- and microplastics in the environment. This is the first study to evaluate contamination by meso- and microplastics in the southern part of the Estuarine Lagoon System and provides information about the nature and extent of contamination by plastics in this estuarine ecosystem.

Spatio-seasonal microplastics distribution along a shallow coastal lagoon ecocline within a marine conservation unit.

The aim of our study is to estimate the abundance and sources of floating microplastics (MPs) along a coastal lagoon ecocline in a marine conservation unit (MCU) for implementing effective prevention and mitigation actions in South Brazil. MPs were sampled monthly, and the abundance and size were determined for hard solids, soft plastic, plastic filaments, and paint fragments. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) analysis was performed, and the MPs were classified as polyethylene, polyester, polypropylene, polybutadiene, and polystyrene. Downstream areas (outside of the MCU) showed high levels of MPs, during the winter, due to low precipitation and the predominance of S/SE winds. During summer, precipitation increases, and MPs are exported with surface runoff, resulting in lower level of MPs. Outside MCU areas, a high concentration of tourism houses, commercials, and artisanal fisheries were observed, which could contribute to all types of plastic debris found in our study.

Ecology of microplastics contamination within food webs of estuarine and coastal ecosystems.

The aim was to describe a methodology developed to study the relationship among the spatio-temporal patterns of habitat utilization, feeding ecology and microplastics (MPs) contamination across the different ontogenetic phases of fishes belonging to different trophic levels and living along the riverine-estuarine-coastal food web. The Goiana Estuary's water column was examined for the seasonal and spatial variation of MPs and their quantification relative to zooplankton, demersal fish species contamination following the same sampling design. The density of MPs in the water column determines their bioavailability. Interest in studies on MPs distribution in relation to spatial and temporal variation of environmental factors and fauna are increasing in quantity and quality. If the ecological strategies presented in this study were replicated in other estuary, comparisons could be made in order to describe how ecosystems work. Standard protocols for sampling, extraction, enumeration and classification of MPs and others pollutant ingested by fishes have been developed and are presented here to encourage comparisons. Standardized and comparable sampling designs and laboratory procedures are an important strategy in order to devise and transfer managerial solutions among different sites and comparisons along time when studying the same environment.

Ingestion of plastic fragments by the Guri sea catfish Genidens genidens (Cuvier, 1829) in a subtropical coastal estuarine system.

One of the most recognized anthropogenic impacts in marine environments is solid waste pollution, especially plastic, which can be ingested by fish, thus interfering with their health. In this context, the aim of this study is to describe the ingestion of plastic fragments and to identify the possible effect of this contamination in the condition factor of Genidens genidens in the Laguna Estuarine System. The stomach contents of 92 G. genidens (26 juveniles and 66 adults) were analyzed. The Index of Relative Importance was performed to identify the contribution of each prey item. Condition factor (CF) was used to analyze the effect of plastic ingestion on the fish's body condition (by comparing individuals in the same ontogenetic phase). For the juveniles, eight items were observed, the most important of which were Penaeidae, followed by Portunidae and plastic. For the adults, 12 items were observed, the most important of which were Penaeidae, Portunidae, Polychaeta, and plastic. The analysis of CF demonstrated higher values for individuals without plastic in the stomach, which indicated a better health condition. The CF of a fish may be affected by variations in the physiological condition, environmental stresses, and nutritional and biological variations, and could be used to compare the body condition or health of a fish species. The ingestion of plastic could significantly influence the worst body condition of the individuals that were analyzed in the present study. The plastic pollution in marine coastal waters is associated with the appropriate waste management levels.

Feeding ecology and ingestion of plastic fragments by Priacanthus arenatus: What's the fisheries contribution to the problem?

Atlantic Bigeye (Priacanthus arenatus) is a demersal species from the Priacanthidae family with little literature relating to its biology and catch aspects. Due to this lack of research, the focus of this effort was to describe the feeding preferences of Atlantic Bigeye and to evaluate the influence of plastic debris derived from the local fisheries activities on its diet. The most important items were Corophiidae, Penaeidae, Actinopterygii, Isopoda, Cephalopoda, Policheta and plastic. Plastic was present in 49.17% of the stomachs analyzed. A total of 210 plastic fragments were found, and 63% were derived from fishing. Of those, 55% were derived from paint fragments from vessels and 8% from synthetic fibers (PA). The results suggest that plastic fragments found in stomachs are related to the species' natural diet and that this debris is locally deposited in the coastal environment. Fishing resources appear to have been affected by this local marine pollution.

Influence of fishing activity over the marine debris composition close to coastal jetty.

Worldwide, the marine debris emissions have been provoking impacts in ecosystems, generating massive mortality of different species with commercial interest. In South America, we have a lack of studies to verify the marine debris composition in transitional environments such as adjacent regions of coastal jetties. These are hydraulic engineering constructions used to maintain the navigation channel access between the sea-estuarine interface and are also used by teleost fishes, crustaceans, and mollusks like artificial shelters (reefs), being excellent fishing grounds. Therefore, the present study was devoted to qualitatively evaluate the composition of marine debris in an internal jetty portion of a Laguna Estuarine System (LES) located in South America (Brazil). Six hundred freediving were conducted to collect marine debris in the study region. The in situ campaigns were performed in 2016 during all spring season (sand substrata) in four distinct zones with 26,400 m2 each one covering almost all adjacent jetty extension, to evaluate possible spatial changes in the marine debris composition. All material obtained was identified, measured, weighed, and ordered in eight groups, with six groups being related to the fishing activity and two groups related to the tourism/community in the study region. So, it was possible to observe possible relations between the marine debris distribution to artisanal and recreational local fishing. After 600 freediving sampling efforts, 2142 marine debris items were obtained, totaling close to 100 kg of solid waste removed from the inner portion of the coastal jetty. Notably, 1752 units (50 kg) of fishing leads were collected being this item the main marine debris residue found in the four sampled areas, corresponding to nearly 50% of the total weight of the collected waste. Ninety-eight percent of marine debris were derived from the local fishing activities, and just 2% were derived from tourism/community. Considering the total contribution related to fishing, 83% of the marine debris were composed by lead (sinkers) adopted by recreational and artisanal fishing. Notably, the catch activity in this region has a close influence over the marine debris composition. Reductions of marine debris emissions derived from the fishing activities have been a global challenge, once this problem is occurring in practically all marine and estuarine environments under the anthropic action. The presence of marine debris changes the local landscape and can provoke serious environmental problems, such as ghost fishing that affects a wide variability of marine mammals, birds, and fishes. Most of marine debris collected came from recreational and artisanal fishing, being the fishing leads the most prominent material, especially in sector 4. This fact is possibly related to the intense mullet fishing using cast nets, usual in this sample area. In the other sectors, there was a great predominance of grapnel fishing leads, widely adopted by recreational fishermen in open water environments. The "fingernails" present in these fishing leads ensure the sinking of the line for a specific location independently of possible flow oscillations of the tidal current and/or currents generated by winds. The massive quantity of fishing leads into the sectors is a dangerous fact. Notably, lead is a heavy, non-biodegradable, and extremely toxic metal that, due to the anthropogenic activities, has been increasing around the world. Future efforts in our study region should evaluate the seasonal marine debris composition to observe possible changes along the different seasons of the year. In this way, it would be possible to infer quantitatively the emission of marine debris derived from the fishing activity, assessing its impacts and enabling the adoption of environmental management strategies. This effort adopted a qualitative analysis, serving to show the current situation of this region that we now know to be vulnerable to the presence of marine debris derived from the fishing activity.

The seasonal and spatial patterns of ingestion of polyfilament nylon fragments by estuarine drums (Sciaenidae).

INTRODUCTION: Artisanal fisheries in tropical estuaries are an important economic activity worldwide. However, gear (e.g. ropes, nets, buoys, crates) and vessels are often in use under dangerous conditions. Polyfilament nylon ropes are used until they are well beyond human and environmental safety limits. Severe wear and tear results in the contamination of the environment with micro-fragments. The spread of these fragments in the marine environment and their ingestion by the biota are documented in the scientific literature and are increasing concerns. The aim of this study was to evaluate the ingestion of plastic fragments by two fish (drum) species in relation to seasonal, habitat and fish size-class variation. MATERIALS AND METHODS: The stomach contents of 569 individuals of Stellifer brasiliensis and Stellifer stellifer from the main channel of the Goiana Estuary were examined to identify variation in the number and the weight of plastic fragments and relate this variation to differences among the seasons (early dry, late dry, early rainy and late rainy), the habitats within the estuary (upper, middle and lower) and the size classes of the fish (juveniles, sub-adults and adults). RESULTS: Plastic fragments were found in 7.9% of the individuals of these two drum species captured from December 2005 to August 2008. Nylon fragments occurred in 9.2% of S. stellifer and 6.9% of S. brasiliensis stomachs. The highest number of nylon fragments ingested was observed in adults during the late rainy season in the middle estuary. DISCUSSION: Blue polyfilament nylon ropes are used extensively in fisheries and can be lost, inappropriately discarded or damaged during use in the estuary. These fragments were the only type of plastic detected during this study. The ingestion of nylon fragments by fish probably occurred during the animals' normal feeding activities. During the rainy season, the discharge of freshwater transports nylon fragments to the main channel and makes the fragments more available to fish. Fishery activities are responsible for a significant amount of the marine debris found in the estuary. CONCLUSIONS: The ingestion of fragments of nylon threads by fish is a demonstrated form of pollution in the Goiana Estuary. The physiological and toxicological consequences of the ingestion of this type of debris are unknown, as is the actual extent of the problem worldwide. The solutions to the problem are in the hands of authorities and communities alike because the good care and timely replacement of gear requires education, investment and effective policies.

Plastic debris ingestion by marine catfish: an unexpected fisheries impact.

Plastic marine debris is a pervasive type of pollution. River basins and estuaries are a source of plastics pollution for coastal waters and oceans. Estuarine fauna is therefore exposed to chronic plastic pollution. Three important catfish species [Cathorops spixii (N=60), Cathorops agassizii (N=60) and Sciades herzbergii (N=62)] from South Western Atlantic estuaries were investigated in a tropical estuary of the Brazilian Northeast in relation to their accidental ingestion of plastic marine debris. Individuals from all three species had ingested plastics. In C. spixii and C. agassizii, 18% and 33% of individuals had plastic debris in their stomachs, respectively. S. herzbergii showed 18% of individuals were contaminated. All ontogenetic phases (juveniles, sub-adults and adults) were contaminated. Nylon fragments from cables used in fishery activities (subsistence, artisanal and commercial) played a major role in this contamination. These catfish spend their entire life cycles within the estuary and are an important feeding resource for larger, economically important, species. It is not yet possible to quantify the scale and depth of the consequences of this type of pollution. However, plastics are well known threat to living resources in this and other estuaries. Conservation actions will need to from now onto take plastics pollution into consideration.

Seasonal differences in mercury accumulation in Trichiurus lepturus (Cutlassfish) in relation to length and weight in a Northeast Brazilian estuary.

BACKGROUND, AIM, AND SCOPE: At tropical latitudes, and especially on the semi-arid coasts of the Brazilian Northeast, the rainfall regime governs the water quality of estuaries due to the pronounced difference between the rainy and dry seasons. These changes may be responsible for seasonal changes in bioavailability of mercury (Hg) and other pollutants to the estuarine and coastal biota. Mercury bioaccumulates along estuarine-marine food chains usually result in higher concentrations in tissues of top predators and posing a risk to both marine mammals and humans alike. The Goiana River Estuary (7.5 degrees S) is a typical estuary of the semi-arid tropical regions and supports traditional communities with fisheries (mollusks, fish, and crustacean). It is also responsible for an important part of the biological production of the adjacent coastal waters. MATERIALS AND METHODS: Trichiurus lepturus (Actinopterygii: Perciformes) is a pscivorous marine straggler. Fish from this species (n = 104) were captured in a trapping barrier used by the local traditional population and using an otter trawl net along the main channel of the low estuary during two dry seasons (D1 = November, December 2005, January 2006; D2 = November, December 2006, January 2007) and the end of a rainy season (R = August, September, October 2006). Fish muscle samples were preserved cold and then freeze-dried prior to analysis of its total mercury (Hg-T) contents. Total mercury was determined by cold vapor atomic absorption spectrometry (CV-AAS) with sodium borohydride as a reducing agent. RESULTS: The studied individuals (n = 104) were sub-adult (30-70 cm, 71 ind.) and adult fish (>70 cm, 33 ind.). Weight (W) (204.1 +/- 97.9 g, total biomass = 21,229.7 g) and total length (TL) (63.1 +/- 10.1 cm, range 29.5-89.0 cm) presented a significant (p < 0.05) correlation. Two-way ANOVA (n = 81) showed that TL and W had significant differences (p < 0.05) among seasons, being higher in D1 than in D2 and R, respectively. Moreover, season vs. month interaction were detected for the variables length and weight. For the variable weight was detected significant difference for the factor month (p < 0.05). It suggests that the fish enter the estuary at the end of the rainy season and increase in length and weight during the time they spend in the estuary. Fish from this estuary are shown to be fit for human consumption (125.3 +/- 61.9 microgHg-T kg(-1) w.wt.; n = 104). Fish mercury contents increased with size and weight. Correlations between TL and Hg-T (r = 0.37286) and between W and Hg-T (r = 0.38212) were significant (p < 0.05). Dryer months showed higher mercury concentrations in fish (D1 773.4 +/- 207.5 microgHg-T kg(-1) d.wt., n = 27; D2 370.1 +/- 78.8 microgHg-T kg(-1) d.wt., n = 27; R 331.2 +/- 138.5 microgHg-T kg(-1) d.wt., n = 27). The variable mercury concentration showed differences in relation to the factor season (p < 0.05), where fish captured during the first dry season showed the highest concentration of mercury. The correlation between Hg-T and rainfall (Rf) showed a negative correlation (r = -0.56; p < 0.05). DISCUSSION: The main likely source of mercury to this estuary is diffuse continental run off, including urban and industrial effluents. Since concentration of mercury in fish tissue is negatively correlated to rainfall, but positively correlated with fish length and weight, it suggests that fish growth in this estuary results in mercury uptake and concentration on the fish tissue. In the dry season of 2005-2006, when rainfall remained below the historic average, fish bioaccumulated significantly more mercury than in the dry season 2006-2007, when rainfall was within the predictable historic average. It is suggested that less rainfall, and consequently less particulate matter and less primary production in the estuary, make mercury more available to the higher levels of the estuarine food chain. In the case of higher rainfall, when river flow increases and water quality in the estuary is reduced, mercury probably is quickly exported associated to the particulate matter to the adjacent coastal waters where it then disperses. This species is a potential routine bioindicator for mercury contamination of the biota, but so far was used only with a limited number of individuals and contexts. CONCLUSIONS: Fish from the Goiana River estuary can still be safely consumed by the local population. However, any further contamination of this resource might lead to total mercury levels above the recommended limits for pregnant women and small children. The proposed heavy dependency of total mercury levels in fish on water quality indicates that land use and water quality standards must be more closely watched in order to guarantee that best possible practices are in place to prevent bioaccumulation of mercury and its transfer along the food chain. Human interventions and climatic events which affect river water flow are also playing a role in the mercury cycle at tropical semi-arid estuaries. RECOMMENDATIONS AND PERSPECTIVES: T. lepturus is largely consumed by coastal populations of tropical and sub-tropical countries all over the world. It is also consumed by a number of marine mammals over which we have a strong conservation interest. This species is also a link among different ecosystems along the estuarine ecocline. Therefore, knowledge of its degree of contamination might contribute to public health issues as well as marine conservation actions. Studies on mercury and other contaminants using this species as bioindicator (cosmopolitan, readily available) could help elucidating mechanisms through which pollutants are being transferred not only through the food chain, but also from estuarine-coastal-open waters. In addition, using the same species in marine pollution studies, especially as part of a mosaic of species, allows for wide range comparisons of marine food chain contamination.