RESUMO
Four adult horses with histories of moderate abdominal pain and inappetence were diagnosed with delayed gastric emptying and gastric impaction attributed to pyloroduodenal obstruction (three cases) or duodenitis (one case). A stapled side-to-side gastrojejunostomy was performed on all horses. Two horses returned to work and survived ≥3 years. One horse was euthanased 6 months post-surgery due to recurrent abdominal pain, and one was found dead 5 months postsurgery after an unattended foaling.
Assuntos
Derivação Gástrica , Doenças dos Cavalos , Cavalos , Animais , Derivação Gástrica/veterinária , Dor Abdominal/etiologia , Dor Abdominal/cirurgia , Dor Abdominal/veterinária , Doenças dos Cavalos/cirurgiaRESUMO
Encephalomyocarditis virus (EMCV) infection was detected by real-time reverse transcription PCR (qRT-PCR) in four adult alpacas (Vicugna pacos) from two properties on the Far North Coast of New South Wales (NSW) in April and May 2018 and in two adult alpacas from a third property on the Central Coast of NSW in October 2018. Viral RNA was detected in a range of samples, including blood, fresh body organs and mucosal swabs. EMCV was isolated from the blood and body organs of five of these alpacas. These animals displayed a range of clinical signs, including inappetence, colic, recumbency and death. Necropsy findings included multifocal to coalescing areas of myocardial pallor, pulmonary congestion and oedema, hepatic congestion and serosal effusion. Histopathological changes comprised acute, multifocal myocardial degeneration and necrosis, with mild, neutrophilic and lymphocytic inflammation (5/5 hearts) and mild, perivascular neutrophilic meningoencephalitis (1/3 brains). This is the first report of disease due to EMCV in alpacas under farm conditions, and it identifies EMCV infection as a differential diagnosis for acute disease and death in this camelid species. In addition to the samples traditionally preferred for EMCV isolation (fresh heart, brain and spleen), blood samples are also appropriate for EMCV detection by qRT-PCR assay.
Assuntos
Camelídeos Americanos , Infecções por Cardiovirus/epidemiologia , Infecções por Cardiovirus/veterinária , Infecções/veterinária , Animais , Vírus da Encefalomiocardite/genética , Coração , New South Wales/epidemiologiaRESUMO
OBJECTIVE: Evaluate the effect of topical 1% cannabidiol on second intention wound healing in distal limb wounds of horses. DESIGN: Experimental. ANIMALS: Six Standardbred horses. METHODS: A total of five 2.5 cm × 2.5 cm full thickness skin wounds were created on the dorsomedial aspect of the metacarpi of 6 horses. Wounds were contaminated with faeces on the day of wound creation. Each wound was then assigned to a treatment group; compounded 1% cannabidiol in unique manuka factor (UMF) 5 manuka honey, UMF 5 manuka honey, UMF 20 manuka honey or saline. Each treatment was applied topically daily for a total of 42 days. Legs were bandaged and bandages were changed, daily, for 13 days postoperatively. Digital photographs of each wound were taken on day 1 then weekly for 6 weeks. Wound size, daily healing rate and total time to healing were recorded and compared statistically. RESULTS: Irrespective of the treatment, wounds did not retract as expected in the first 7 days after wound creation. There was no difference in wound area, daily healing rate, days to complete healing between treatment groups. CONCLUSIONS: This preliminary study failed to demonstrate any difference in wound healing variables between treatment groups in this model of second intention wound healing. This was unexpected due to the established effects of UMF 20 manuka honey on wound healing using the same model. This may be due to systemic effects of cannabidiol and study design. Further research into the use of cannabidiol in equine wounds is warranted.
Assuntos
Fator V , Mel , Animais , Canabidiol , Cavalos , Intenção , Extratos Vegetais , CicatrizaçãoRESUMO
OBJECTIVE: To quantify the time to clear dexamethasone from plasma and urine of horses following a single nebulisation. DESIGN: Experimental using six Standardbred mares. METHODS: Dexamethasone sodium phosphate (0.04 mg/kg) diluted in 0.9% sodium chloride was administered as an aerosol using a Flexineb E2® nebuliser. Blood samples (0, 2, 4, 6, 8, 10, 12, 24, 32, 48, 72 and 96 h) and urine samples (0, 1, 4, 8, 24, 32, 48, 72 and 96 h) were collected for analysis using liquid chromatography mass spectrometry. RESULTS: Maximum plasma concentrations (tmax ) were reached by the earliest detection point (2 h) after nebulisation (0.6-1.8 ng/mL), but was no longer detectable at 48 h. However, in one horse 0.1 ng/mL was found at 96 h after three consecutive readings of 0 ng/mL. The tmax in urine was reached by the earliest collection point (1 h) after nebulisation (3.2-23.8 ng/mL), but was no longer present in urine at 72 h in five horses, while detectable levels (0.1 ng/mL) were still present at 96 h in one horse. CONCLUSIONS: A single dose of 0.04 mg/kg of DSP administered as an aerosol through a FlexinebE2® mask was no longer detectable in blood at 48 h in six horses tested, but one horse returned a reading of 0.1 ng/mL at 96 h after having no detectable levels. Dexamethasone was not detectable in urine at 72 h in five horses but was detectable at a low concentration (0.1 ng/mL) at 96 h in one horse.
Assuntos
Anti-Inflamatórios/sangue , Anti-Inflamatórios/urina , Dexametasona/sangue , Dexametasona/urina , Cavalos/sangue , Cavalos/urina , Animais , Anti-Inflamatórios/uso terapêutico , Asma/tratamento farmacológico , Asma/veterinária , Dexametasona/uso terapêutico , Feminino , Doenças dos Cavalos/tratamento farmacológico , Nebulizadores e Vaporizadores/veterinária , Projetos Piloto , Distribuição AleatóriaRESUMO
AIMS To compare the effect of oxygen supplementation using flow-by or a face mask on the partial pressure of arterial oxygen (PaO2) in sedated dogs. METHODS Twenty healthy dogs weighing >15â kg, of mixed sex and breed, were enrolled in a randomised cross-over study. Each dog was sedated with I/M 0.015â mg/kg medetomidine and 0.5â mg/kg methadone. Twenty minutes later dogs were exposed to two 5-minute treatment periods of oxygen supplementation separated by a 15-minute washout period during which dogs were allowed to breathe room air. During the treatment periods, oxygen was delivered at a flow rate of 3â L/minute either through a face mask (face mask oxygenation), or via a tube held 2â cm from the dog's nares (flow-by oxygenation). The order in which the treatments were administered was randomised. Arterial blood was collected for blood gas analysis and rectal temperature measured at four times: prior to commencing treatments, after each treatment, and at the end of the 15 minutes washout period between treatments. RESULTS The mean PaO2 in arterial samples taken from the dogs after face mask oxygen supplementation was 371.3 (SE 13.74) mmHg which was higher than in samples taken after they received flow-by oxygen supplementation (182.2 (SE 6.741) mmHg; p<0.001). The mean PaO2 in samples taken after receiving either form of oxygen supplementation was higher than in samples taken after the dogs had been breathing room air (82.43 (SE 2.143) mmHg; p<0.001). There was no association between sex, age, weight or breed of dogs and blood gas parameters or rectal temperature (p>0.05). CONCLUSIONS Oxygen supplementation delivered using a face mask was more effective at increasing PaO2 than flow-by oxygen supplementation. Flow-by oxygen supplementation at a distance of 2â cm from the nose may be a suitable alternative when the use of a face mask is not tolerated by the patient.
Assuntos
Máscaras/veterinária , Oxigenoterapia/veterinária , Oxigênio/administração & dosagem , Animais , Artérias , Gasometria/veterinária , Cães , Feminino , Hipnóticos e Sedativos/administração & dosagem , Funções Verossimilhança , Masculino , Medetomidina/administração & dosagem , Metadona/administração & dosagem , Entorpecentes/administração & dosagem , New South Wales , Oxigenoterapia/métodos , Pressão Parcial , Distribuição AleatóriaAssuntos
Camelídeos Americanos/lesões , Procedimentos Ortopédicos/veterinária , Luxação Patelar/veterinária , Animais , Coxeadura Animal/etiologia , Coxeadura Animal/cirurgia , Masculino , Luxação Patelar/etiologia , Luxação Patelar/cirurgia , Ferimentos e Lesões/complicações , Ferimentos e Lesões/veterináriaRESUMO
OBJECTIVE: To compare the effects of two concentrations of oxygen delivered to the anaesthetic breathing circuit on oxygenation in mechanically ventilated horses anaesthetised with isoflurane and positioned in dorsal or lateral recumbency. METHODS: Selected respiratory parameters and blood lactate were measured and oxygenation indices calculated, before and during general anaesthesia, in 24 laterally or dorsally recumbent horses. Horses were randomly assigned to receive 100% or 60% oxygen during anaesthesia. All horses were anaesthetised using the same protocol and intermittent positive pressure ventilation (IPPV) was commenced immediately following anaesthetic induction and endotracheal intubation. Arterial blood gas analysis was performed and oxygenation indices calculated before premedication, immediately after induction, at 10 and 45 min after the commencement of mechanical ventilation, and in recovery. RESULTS: During anaesthesia, the arterial partial pressure of oxygen was adequate in all horses, regardless of position of recumbency or the concentration of oxygen provided. At 10 and 45 min after commencing IPPV, the arterial partial pressure of oxygen was lower in horses in dorsal recumbency compared with those in lateral recumbency, irrespective of the concentration of oxygen supplied. Based on oxygenation indices, pulmonary function during general anaesthesia in horses placed in dorsal recumbency was more compromised than in horses in lateral recumbency, irrespective of the concentration of oxygen provided. CONCLUSION: During general anaesthesia, using oxygen at a concentration of 60% instead of 100% maintains adequate arterial oxygenation in horses in dorsal or lateral recumbency. However, it will not reduce pulmonary function abnormalities induced by anaesthesia and recumbency.
Assuntos
Cavalos/fisiologia , Oxigênio/administração & dosagem , Oxigênio/análise , Postura/fisiologia , Respiração/efeitos dos fármacos , Anestesia Geral , Anestésicos Inalatórios/farmacologia , Animais , Pressão Arterial/efeitos dos fármacos , Gasometria/veterinária , Feminino , Isoflurano/farmacologia , Modelos Lineares , Masculino , Respiração Artificial/veterinária , Decúbito DorsalRESUMO
AIMS To compare the efficacy of an enteric coated esomeprazole paste with an enteric coated omeprazole paste to increase gastric pH after oral administration in horses. METHODS Nine adult Standardbred horses were randomly assigned to three groups, each containing three horses, for a study comprising three phases of 10 days, with an 18-day washout period between each phase. In each phase, three horses received either 0.5â mg/kg esomeprazole, 1â mg/kg omeprazole or a placebo, as an oral paste, once daily for 10 days (Days 0-9). Over the course of study all horses received all three treatments. Gastric fluid samples were collected using a gastroscope on Days 1, 3, 5, 8 and 10, with food and water withheld for 16 hours prior to collection of samples. The pH of all samples was measured immediately after collection. RESULTS Mean pH (3.38; SD 1.75) of the gastric fluid samples in the horses that received the placebo was lower than in the horses that received esomeprazole (6.28; SD 1.75) or omeprazole (6.13; SD 1.75) (p<0.001). There was no difference in the mean pH between horses receiving esomeprazole and those receiving omeprazole (p=0.56). CONCLUSIONS AND CLINICAL RELEVANCE Under these study conditions, esomeprazole paste was equally as effective as omeprazole paste in increasing gastric pH in horses. Enteric coated esomeprazole, may be a therapeutic alternative to omeprazole for the prevention of gastric ulcers in horses.
Assuntos
Antiulcerosos/uso terapêutico , Esomeprazol/uso terapêutico , Doenças dos Cavalos/tratamento farmacológico , Omeprazol/uso terapêutico , Animais , Cavalos , Concentração de Íons de Hidrogênio , Projetos Piloto , Distribuição Aleatória , Resultado do TratamentoRESUMO
OBJECTIVE: To compare the effect of application of manuka honey with unique manuka factor (UMF) 5 or 20 with a generic multifloral honey on equine wound healing variables. METHODS: Two full-thickness skin wounds (2.5 × 2.5 cm) were created on the metatarsus of both hindlimbs of eight Standardbred horses. The wounds on each horse were assigned to 1 of 4 treatments: UMF20 (UMF20) and UMF5 (UMF5) manuka honey; generic multifloral honey (GH); and a saline control. Bandages were changed daily for 12 days, after which treatment was stopped and the bandages were removed. Wound area was measured on day 1, then weekly until day 42. Overall wound healing rate (cm2 /day) and time to complete healing were recorded. RESULTS: There was no difference in wound area for any of the treatments on any measurement day except for day 21, where the mean wound area for wounds treated with UMF20 was smaller than the mean wound area for the UMF5-treated wounds (P = 0.031). There was no difference in mean (± SE) overall healing rate (cm2 /day) among the treatment groups. There were differences in mean (± SE) days to complete healing. Wounds treated with UMF20 healed faster than wounds treated with GH (P = 0.02) and control wounds (P = 0.01). CONCLUSIONS: Treatment of wounds with UMF20 reduced overall wound healing time compared with wounds treated with GH and control wounds. However, using this model the difference in the overall time to complete healing was small.
Assuntos
Mel , Cavalos/lesões , Cicatrização/efeitos dos fármacos , Cicatrização/fisiologia , Animais , BandagensRESUMO
CASE REPORT: A newborn foal was referred for evaluation because it had not passed meconium, despite the administration of four enemas. Abdominal radiographs and ultrasound scans showed generalised gaseous distension of the intestine and there was no observable meconium in the colon. Positive contrast colography showed contrast medium extending to the transverse colon. An exploratory laparotomy confirmed the absence of the left and right dorsal colon and the pelvic and diaphragmatic flexures. An end-to-side anastomosis of the left ventral colon to the midpoint of the small colon was performed. The foal recovered from anaesthesia and surgery uneventfully and immediately began suckling from the mare, with no signs of abdominal pain in the postoperative period. The foal began to pass soft faeces 3 days after surgery and at 6 months after surgery the foal was clinically normal and growing at a similar rate to its cohort. CONCLUSION: Intestinal atresia is a rare condition in foals, but should be considered as a differential diagnosis in foals that fail to pass meconium. Early recognition and surgical intervention can offer an improved chance of short-term survival in cases where there is adequate intestine to anastomose. An end-to-side anastomosis technique can be used where an end-to-end technique is not practical because of the difference in diameter of the proximal and distal intestinal segments.
Assuntos
Colo/anormalidades , Doenças dos Cavalos/congênito , Atresia Intestinal/veterinária , Anastomose Cirúrgica/veterinária , Animais , Colo/cirurgia , Doenças dos Cavalos/diagnóstico por imagem , Doenças dos Cavalos/cirurgia , Cavalos , Atresia Intestinal/diagnóstico por imagem , Atresia Intestinal/cirurgia , Masculino , Mecônio , New South Wales , Resultado do TratamentoRESUMO
Metabolic heat produced by Thoroughbred racehorses during racing can rapidly elevate core body temperature (1°C/min). When environmental conditions are hot and humid, the normal physiological cooling mechanisms become ineffective. The heat accumulated may exceed a critical thermal maximum (estimated to be 42°C), which may trigger a complex pathophysiological cascade with potentially lethal consequences. This syndrome has been labelled exertional heat illness (EHI). EHI is described in humans, but has not been well documented in Thoroughbred racehorses. The clinical signs described in racehorses would suggest that the pathophysiological events affecting the central nervous (CNS) and gastrointestinal systems are similar to those described in humans. Clinical signs are progressive and include signs of endotoxaemia and increasing levels of CNS dysfunction. Initially, horses that may be mildly irritable (agitated, randomly kicking out) may progress to unmanageable (disorientation, severe ataxia, falling) and ultimately convulsions, coma and death. Currently, the approach to treatment is largely empirical and involves rapid and effective cooling, administration of drugs to provide sedation, administration of non-steroidal anti-inflammatory drugs to ameliorate the effects of endotoxaemia and glucocorticoids to stabilise cell membranes and reduce the effects of inflammation on the CNS. This review provides an overview of the current knowledge about EHI in Thoroughbred racehorses, suggests a likely pathophysiology of the syndrome in horses based on the current literature on heat illness in humans and horses, and outlines current treatment strategies being used to treat racehorses with clinical signs of EHI.
Assuntos
Transtornos de Estresse por Calor/veterinária , Doenças dos Cavalos/etiologia , Temperatura Alta/efeitos adversos , Animais , Transtornos de Estresse por Calor/etiologia , Cavalos/fisiologia , Esforço Físico/fisiologia , Fatores de Risco , Síndrome , Clima TropicalRESUMO
OBJECTIVE: To investigate the effect of 66% Manuka honey gel on the concentrations of transforming growth factor (TGF)-ß1 and TGF-ß3, bacterial counts and histomorphology during healing of contaminated equine distal limb wounds. METHODS: In this experimental study of 10 Standardbred horses, five full-thickness skin wounds (2 × 1.5 cm) were created on one metacarpus and six similar wounds were created on the contralateral metacarpus. Wounds were assigned to three groups: non-contaminated control wounds; contaminated control wounds; contaminated wounds treated daily with 1 mL Manuka honey gel topically for 10 days. For the contaminated wounds, faeces were applied for 24 h after wound creation. In five horses wounds were bandaged and in the other five horses wounds were left without a bandage. Biopsies were taken on days 1, 2, 7 and 10 after wounding to evaluate the effects of Manuka honey gel, wound contamination and bandaging on TGF-ß1 and TGF-ß3 concentrations, aerobic and anaerobic bacterial counts, and histomorphology. RESULTS: Manuka honey gel had no significant effect on TGF-ß1 and TGF-ß3 concentrations or wound bacterial counts. Manuka honey gel decreased wound inflammation (days 7, 10), increased angiogenesis (days 2, 7, 10), increased fibrosis and collagen organisation (day 7) and increased epithelial hyperplasia (days 7, 10). CONCLUSIONS: Treatment with Manuka honey gel resulted in a more organised granulation tissue bed early in wound repair, which may contribute to enhanced healing of equine distal limb wounds.
Assuntos
Mel , Doenças dos Cavalos/terapia , Pele/lesões , Fator de Crescimento Transformador beta1/metabolismo , Fator de Crescimento Transformador beta3/metabolismo , Administração Tópica , Animais , Carga Bacteriana , Extremidades , Fezes/microbiologia , Doenças dos Cavalos/metabolismo , Doenças dos Cavalos/microbiologia , Cavalos , Pele/microbiologia , CicatrizaçãoRESUMO
OBJECTIVE: To investigate the effect of activated protein C (APC) on second intention healing of distal limb wounds in horses. METHODS: In this experimental study of eight Standardbred geldings, six full-thickness skin wounds (2 × 1.5 cm) were created on one metacarpus (biopsy limb) and five similar wounds were created on the contralateral metacarpus (photographed limb). Three wounds on the biopsy limb were treated topically with 190 µg APC on days 1, 3, 6 and 9, while the remaining three wounds were untreated (control). One treated and one control wound were biopsied on days 4, 7 and 11 for histopathology. Wounds on the photographed limb were treated with either 66% Manuka honey gel, a commercial antibiotic ointment (bacitracin-neomycin-polymixin B ointment; BNP) or petrolatum daily throughout healing, treated on days 1,3,6 and 9 with 190 µg APC or left untreated. These wounds were digitally photographed and the wound area measured on day 1, then weekly until day 49. Overall time to healing was recorded. RESULTS: There was no effect of APC on wound size, the rate of healing or the overall time to heal. However, compared with control wounds, histological scoring demonstrated enhanced epithelialisation (day 4) and angiogenesis (day 11). Wound healing variables for wounds treated with APC, Manuka honey gel and control wounds were not different and the variables for wounds treated with BNP and petrolatum demonstrated delayed healing. CONCLUSION: The improvements in histological scores in APC-treated wounds suggest further study into the effect of APC on second intention wound healing in horses is warranted.
Assuntos
Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Proteína C/farmacologia , Pele/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Animais , Bacitracina/farmacologia , Combinação de Medicamentos , Géis , Mel , Cavalos , Extremidade Inferior/lesões , Extremidade Inferior/fisiopatologia , Masculino , Neomicina/farmacologia , Fotografação , Polimixina B/farmacologia , Distribuição Aleatória , Proteínas Recombinantes/farmacologia , Pele/lesões , Cicatrização/fisiologiaRESUMO
OBJECTIVE: To survey veterinary practitioners in Australia on how they administer pentosan polysulfate (PPS) to horses and their perceptions of the efficacy of PPS for: the prevention and treatment of osteoarthritis (OA), the treatment of OA when PPS is combined with other drugs, and the efficacy of PPS compared with other disease-modifying osteoarthritic drugs. DESIGN: Practitioners were contacted by email, which contained a link to an online survey. RESULTS: A total of 76 responses (34.5%) to the survey were received. Respondents most commonly used PPS as prophylactic therapy prior to competition (80.3%). As a prophylactic agent, PPS was considered by 48.2% of respondents to have high efficacy. The most common dose regimen for prevention and treatment of OA was 3 mg/kg, intramuscularly, once weekly for 4 weeks followed by monthly injections. Most respondents (78%) combined PPS with other drugs for treatment of OA. Intra-articular corticosteroids and hyaluronate (HA) was the most common drug combination used with PPS. PPS was preferred as a prophylactic agent when compared with HA (88.7% vs 11.3%). For treating OA, 83% of respondents considered a combination of PPS, HA and glucosamine to be more efficacious than PPS alone. However, the most common reason not to use this combination was cost (79.1%). CONCLUSION: All respondents used PPS for prophylaxis and/or treatment of OA despite limited published scientific evidence proving its efficacy in horses. Further research is necessary to provide evidence of the clinical efficacy of PPS for the prevention and treatment of OA in horses.
Assuntos
Anticoagulantes/uso terapêutico , Doenças dos Cavalos/tratamento farmacológico , Osteoartrite/veterinária , Poliéster Sulfúrico de Pentosana/uso terapêutico , Animais , Austrália , Glicosaminoglicanos/uso terapêutico , Inquéritos Epidemiológicos , Doenças dos Cavalos/prevenção & controle , Cavalos , Humanos , Osteoartrite/tratamento farmacológico , Osteoartrite/prevenção & controle , Resultado do Tratamento , Médicos Veterinários , Medicina VeterináriaRESUMO
OBJECTIVE: Evaluate synovial fluid cytology, total protein concentration and viscosity after intra-articular administration of combined pentosan polysulfate and glucosamine in horses. DESIGN: Ten adult Standardbred horses had each carpal joint (n = 20) randomly assigned to one of two treatments: control (5 mL saline) or treated (pentosan polysulfate and glucosamine). All horses received an intra-articular injection every 7 days for 3 weeks and synovial fluid samples were collected on days 1 (baseline), 2, 3, 7, 8, 9, 14, 15, 16 and 21. Synovial fluid variables measured included total protein concentration, total nucleated cell count, red blood cell count, the neutrophil, lymphocyte, mononuclear cell and eosinophil percentages, and viscosity. RESULTS: Following injection, the total nucleated cell count increased in the synovial fluid samples from both groups. Values were significantly higher in treated joints on days 2, 3, 15 and 16. Red blood cell counts were low and no differences were seen between treated and control joints. Total protein concentrations and neutrophil percentages increased in both groups after injection, but decreased towards normal ranges within 7 days. Total protein concentration was significantly higher in treated joints on days 1, 3, 8, 9 and 15. Percentages of neutrophils were higher in treated joints on day 2, but significantly lower on days 8 and 15. Viscosity was significantly higher in treated joints on day 2 only. CONCLUSIONS: Intra-articular administration of combined pentosan polysulfate and glucosamine causes a mild inflammatory synovitis that is not substantially different to that elicited by injection of a similar volume of saline and so we conclude that these drugs are safe to use in the horse.
Assuntos
Glucosamina/farmacologia , Injeções Intra-Articulares/veterinária , Poliéster Sulfúrico de Pentosana/farmacologia , Líquido Sinovial , Animais , Anticoagulantes/farmacologia , Proteínas Sanguíneas/análise , Proteínas Sanguíneas/efeitos dos fármacos , Carpo Animal , Doenças dos Cavalos/induzido quimicamente , Cavalos , Líquido Sinovial/química , Líquido Sinovial/citologia , Líquido Sinovial/efeitos dos fármacos , Sinovite/induzido quimicamente , Sinovite/veterinária , Viscosidade/efeitos dos fármacosRESUMO
OBJECTIVE: To evaluate the effect of collecting serial tracheal aspirate (TA) and bronchoalveolar lavage (BAL) samples on the cytological findings of subsequent fluid samples obtained from horses without clinical signs of respiratory disease. STUDY DESIGN: Experimental. STUDY POPULATION: Six healthy Standardbred horses. METHODS: Endoscopically-guided TA samples, and BAL samples collected using the blind field technique were obtained from the six horses on days 1, 2, 3, 4, 5, 12, and 17. On day 17, horses were sampled three times: at baseline and at 2.5 h and 4 h apart. The differential cytology of the fluid samples collected at each time point was expressed as percentages and compared statistically. RESULTS: There was a significant increase in neutrophil percentage in the TA samples taken at day 17 (at 2.5 h but not at 4 h apart). There was no significant change in the neutrophil percentages in the TA samples when repeated samples were taken ≥ 24 h apart. There was no significant change in the neutrophil percentages in the BAL fluid at any collection point. There were inconsistent changes in the percentages of lymphocytes and macrophages in the BAL fluid over time, but these remained within normal reference ranges and were considered clinically insignificant. CONCLUSIONS: Serial TA and BAL samples can be taken at 24 h intervals without affecting the cytological findings of subsequent fluid samples collected using the techniques described.
Assuntos
Líquido da Lavagem Broncoalveolar/citologia , Doenças dos Cavalos/diagnóstico , Neutrófilos/citologia , Doenças Respiratórias/veterinária , Traqueia/citologia , Animais , Broncoscopia/veterinária , Endoscopia/veterinária , Feminino , Doenças dos Cavalos/patologia , Cavalos , Linfócitos/citologia , Macrófagos/citologia , Masculino , Doenças Respiratórias/diagnóstico , Doenças Respiratórias/patologia , Fatores de TempoRESUMO
OBJECTIVE: Review 103 cases of presumed tick envenomation in horses. DESIGN: Retrospective study. METHOD: Variables, including date of presentation, age, breed, weight, presence of ticks, gait and respiration scores, duration of recumbency, treatment, outcome and complications were recorded. A series of univariable screening tests were performed and used in a multivariable logistic regression model. RESULTS: There were a total of 103 cases affecting 10 breeds, aged between 1 week and 18 years of age. Horses >6 months old and weighing >100 kg had a higher odds of death than those <6 months old and <100 kg. Cases were seen from North Queensland to the central coast of New South Wales and were more likely to present in the warmer months. There was no association between the number of ticks found on an animal and death. Horses with a higher respiratory score had higher odds of dying, but there was no association between gait score and survival. Horses recumbent >120 h after presentation had higher odds of dying. Complications were reported in 35% of horses. The odds ratio for survival was higher for horses receiving >0.5 mL/kg of tick antiserum. Overall, 74% of horses survived. Multivariable modelling was limited by the small sample size. CONCLUSION: In general, tick envenomation in horses follows the geographic distribution of Ixodes holocyclus. Tick antiserum administered at >0.5 mL/kg increases the odds of survival. It would appear that the complications associated with managing a recumbent horse increase the odds of death.
Assuntos
Venenos de Artrópodes/toxicidade , Doenças dos Cavalos/mortalidade , Ixodes , Toxicoses por Carrapatos/veterinária , Animais , Feminino , Cavalos , Masculino , Estudos Retrospectivos , Fatores de Risco , Estações do Ano , Infestações por Carrapato/mortalidade , Infestações por Carrapato/veterinária , Toxicoses por Carrapatos/mortalidadeRESUMO
CASE HISTORY: An 8-week-old, 16-kg alpaca cria was presented with a 2-week history of acute onset of left forelimb lameness. CLINICAL FINDINGS: The cria was weight-bearing but lame at the walk in the left forelimb. There was swelling, heat and pain on palpation of the soft tissues, extending from the mid-shaft of the humerus to the elbow. There was no evidence of a traumatic injury. Radiologically, there was evidence of osteomyelitis and a sequestrum involving the distal metaphysis of the humerus. The sequestrum was removed surgically. No bacteria were cultured from samples taken during surgery. After an initial improvement, the cria became acutely lame, and developed an abscess at the surgical site, which was drained. A pure growth of a strictly anaerobic, Gram-negative, non-spore-forming, pleomorphic, filamentous rod was cultured from fluid from the abscess. Further radiographs suggested a second sequestrum was present, and this was removed surgically. Apart from partial dehiscence of the wound, the cria recovered well, and was sound and without complications 18 months after surgery. DIAGNOSIS: Osteomyelitis and formation of sequestra in the distal metaphysic of the left humerus. CLINICAL RELEVANCE: There has only been one case of osteomyelitis and bone sequestration without evidence of trauma in alpaca crias reported in the literature. However, anecdotal reports by veterinary practitioners described similar cases of osteomyelitis and bone sequestration, usually affecting the long bones. Further investigation of osteomyelitis and bone sequestration in crias is needed, to define the incidence, determine the pathogenesis, and develop specific recommendations for prevention and treatment.
Assuntos
Camelídeos Americanos , Membro Anterior/patologia , Osteomielite/veterinária , Animais , Antibacterianos/uso terapêutico , Feminino , Membro Anterior/diagnóstico por imagem , Membro Anterior/cirurgia , Osteomielite/tratamento farmacológico , Osteomielite/cirurgia , RadiografiaRESUMO
Two horses presented with a history of severe lameness that was localised to the area around the second phalanx (P2) of one forelimb. Radiographs of the second phalanx of both horses were unremarkable, apart from some smooth periosteal new bone production on the dorsum of P2. Magnetic resonance imaging revealed a non-displaced fracture of P2 in both horses. One horse was euthanased because of a poor prognosis for racing, but the second horse was treated conservatively and salvaged for breeding. Magnetic resonance imaging is a newer imaging modality in horses and may be useful in diagnosing cases of obscure lameness.