Efficacy of Probiotics in Overweight and Obesity Control: An Umbrella Review and Subgroup Meta-Analysis.

Numerous primary and secondary studies have consistently demonstrated that probiotics, including lactobacillus and Bifidobacterium, possess a potential anti-obesity effect. However, it is worth noting that some studies have yielded contrasting results. Considering this, our study aims to present a comprehensive overview of published systematic reviews and meta-analyses, focusing on the efficacy and safety of probiotics in managing obesity. To achieve this objective, we conducted an umbrella review following the PRISMA protocol and Cochrane guidelines. We searched databases such as Embase, PubMed, Cochrane Library, and Google Scholar for relevant systematic reviews and meta-analyses published in English, without imposing any date restrictions. Our inclusion criteria encompassed studies evaluating the anti-obesity impact of probiotics, with a specific focus on changes in body mass index (BMI), fat mass percentage (FMP), body weight (BW), and body fat mass (BFM). These studies were meticulously reviewed by two independent reviewers. Our analysis included five systematic reviews and 18 meta-analyses that met the predefined inclusion and exclusion criteria. The meta-analyses revealed statistically significant reductions in the following parameters: BMI, a decrease of 0.30 kg/m2 (p < 0.00001, 95% CI - 0.36 to - 0.25); BFM, a reduction of 0.86 kg (p < 0.00001, 95% CI - 1.02 to - 0.71); BW, a decrease of 0.59 kg (p < 0.00001, 95% CI - 0.74 to - 0.44); and FMP, a substantial decline of 78% (p < 0.00001, 95% CI - 1.02 to - 0.54). In summary, our umbrella review suggests that existing evidence supports the potential benefits of probiotics in managing obesity and overweight. However, it is essential to acknowledge that the credibility of this evidence is somewhat limited due to the inclusion of studies with poor-quality designs and relatively small participant numbers. To establish the true efficacy of probiotics in obesity management, we recommend conducting robust studies involving larger participant cohorts.

Antiretroviral therapy and the risk of sexual transmission of HIV: a systematic review and meta-analysis.

OBJECTIVES: The aim of this study was to evaluate the effect of antiretroviral therapy (ART) with or without condom use on the risk of sexual transmission of HIV. METHODS: A search of the literature was conducted in the PubMed, Web of Science, Cochrane, SCOPUS and EMBASE databases for studies dating back to 2008. The results were summarized as relative risks and incidence rates with 95% confidence intervals (CIs). The effect sizes were pooled using random-effects models and heterogeneity was evaluated using the Cochrane Q test and I2 . RESULTS: Of the 1424 studies retrieved in the initial search, 10 met the eligibility requirements. ART was associated with a 52% reduction in transmission risk compared to no ART, with a relative risk of 0.48 (95% CI 0.439-0.525) (Q = 0.524; I2 = 0.0%; overall effect Z = 15.99, P < 0.0001). ART vs. no ART caused a reduction in the risk from 5.6 person-years (95% CI 3.26-9.62 person-years) (Q = 0.771; I2 = 0.0%; overall effect Z = 6.25, P < 0.0001) in the untreated group to 0.85 person-years (95% CI 0.28-2.99 person-years) (Q = 0.038; I2 = 76.7%; overall effect Z = 0.11, P = 0.772) in the treated group, but not significantly so, which is equivalent to an 84% reduction in the risk of sexual transmission. In accordance with the Swiss National AIDS Commission declaration, ART with suppressed viral loads was associated with a minimal risk of transmission, with a median time at risk of 0.00 person-years (95% CI 0.00-0.00 person-years) (Q = 1.00; I2 = 0.0%; overall effect Z = 6.80, P < 0.0001). CONCLUSIONS: ART and condom use were found to cause a significant reduction in the risk of sexual transmission of HIV in both homosexual and heterosexual populations, based on previous systematic reviews and meta-analyses.

Chemical composition and antifungal activity of the essential oil of Zhumeria majdae, Heracleum persicum and Eucalyptus sp. against some important phytopathogenic fungi.

Application of essential oils of medicinal plants is considered a safe and acceptable method for plant disease management to protect plants from pathogenic microorganisms. Thus, in recent study, essential oils (EOs) from Zhumeria majdae, Heracleum persicum (two Iranian endemic plants) and Eucalyptus sp. were assayed for their antifungal potential against ten phytopathogenic fungi, including Fusarium graminearum, Fusarium asiaticum, Fusarium redolens f.sp. dianthus, Fusarium verticillioides, Fusarium oxysporum f.sp. lentis, Sclerotinia sclerotiorum, Aspergillus flavus, Aspergillus tubingensis, Botrytis cinerea and Cladosporium cladosporioides. Chemical composition of these oils was identified by GC-MS analysis. Based on our results, Z. majdae essential oil exhibited the best antifungal activity among tested essential oils, completely inhibiting growth of five fungal species. EOs of Eucalyptus sp. and H. persicum showed moderate and poor antifungal capacity, respectively. GC-MS analysis demonstrated that linalool and camphor were the main components of the essential oils of Z. majdae; furthermore, 1,8-cineole and hexyl ester formed the major portions of Eucalyptus sp. and H. persicum EOs. Due to the significant inhibition of some EOs, additional research about their use for control of plant diseases caused by these fungi is recommended.

Sperm DNA methylation of H19 imprinted gene and male infertility.

Infertility affects up to 15% of reproductive-aged couples worldwide, with male factor being detected in 40%-50% of the cases. Proper sperm production is associated with the establishment of appropriate epigenetic marks in developing germ cells. Several studies have demonstrated the association between abnormal spermatogenesis and epigenetic disturbances with the major focus on DNA methylation. Imprinted genes are expressed in a parent-of-origin-specific manner, and the role of their DNA methylation in proper spermatogenesis has been documented recently. The existing evidence along with the absence of relevant data in south of Iran prompted us to study the methylation of H19 imprinted gene in spermatozoa of idiopathic infertile patients (males with abnormalities in sperm parameters) and healthy controls by Combined Bisulfite Restriction Analysis. According to our results, the lowest methylation percentage of H19 imprinted gene belongs to three cases with sperm characteristics under normal range (two cases Oligoasthenoteratozoospermia and one case Oligoteratozoospermia). However, our results show that the median of methylation percentage for H19 is not statistically significant between case and control groups. Our results and those of others introduce DNA methylation as a potential marker of fertility and should be investigated with more patients in future studies.

Aggressive Angiomyxoma of the Bladder Neck Requiring Local Excision and Mitrofanoff Formation.

Aggressive angiomyxoma is a rare mesenchymal tumour predominantly affecting the female pelvis and perineum but has also been described in males. This tumour can often present a diagnostic challenge and has a propensity for local recurrence after surgical excision. We present an unusual case of aggressive angiomyxoma arising from the bladder of a female patient which required local excision and Mitrofanoff formation.

Male reproductive health under threat: Short term exposure to radiofrequency radiations emitted by common mobile jammers.

BACKGROUND: Modern life prompted man to increasingly generate, transmit and use electricity that leads to exposure to different levels of electromagnetic fields (EMFs). Substantial evidence indicates that exposure to common sources of EMF such as mobile phones, laptops or wireless internet-connected laptops decreases human semen quality. In some countries, mobile jammers are occasionally used in offices, shrines, conference rooms and cinemas to block the signal. AIMS: To the best of our knowledge, this is the first study to investigate the effect of short term exposure of human sperm samples to radiofrequency (RF) radiations emitted by common mobile jammers. SUBJECTS AND METHODS: Fresh semen samples were collected by masturbation from 30 healthy donors who had referred to Infertility Treatment Center at the Mother and Child Hospital with their wives. Female problem was diagnosed as the reason for infertility in these couples. STATISTICAL ANALYSIS: T-test and analysis of variance were used to show statistical significance. RESULTS: The motility of sperm samples exposed to jammer RF radiation for 2 or 4 h were significantly lower than those of sham-exposed samples. These findings lead us to the conclusion that mobile jammers may significantly decrease sperm motility and the couples' chances of conception. CONCLUSION: Based on these results, it can be suggested that in countries that have not banned mobile jammer use, legislations should be urgently passed to restrict the use of these signal blocking devices in public or private places.

A 2-year follow-up of spirometric parameters in workers of a tile and ceramic industry, Yazd, southeastern Iran.

BACKGROUND: Respiratory diseases cause a considerable amount of morbidity and mortality in the world. Pulmonary function tests are important measures for the diagnosis and management of respiratory disorders. Workers in tile and ceramic industry are exposed to high amounts of respiratory pollutants. OBJECTIVE: To identify the changes in spirometric parameters in a 2-year period among tile and ceramic workers in Yazd and compare it with a control group. METHODS: The study was conducted in 5 tile and ceramic factories selected by cluster sampling between 2009 and 2011 in Yazd, southeastern Iran. Demographic data and spirometric parameters of participants were recorded. RESULTS: Spirometric parameters were significantly reduced during the 2 years. The largest decrease was observed in FVC (≈500 mL) in ball-mill and grinding after 2 years. Decrease in all spirometric parameters was significantly higher in industrial workers than office workers. CONCLUSION: Respiratory exposure in tile and ceramic industry can significantly affect pulmonary function tests.

Health care financing in iran; is privatization a good solution?

BACKGROUND: This paper considers a range of issues related to the financing of health care system and relevant government policies in Iran. METHODS: This study used mixed methods. A systematic literature review was undertaken to identify relevant publications. This was supplemented by hand searching in books and journals, including government publications. The issues and uncertainties identified in the literature were explored in detail through semi-structured interviews with key informants. These were triangulated with empirical evidence in the form of the literature, government statistics and independent expert opinions to validate the views expressed in the interviews. RESULTS: The systematic review of published literature showed that no previous publication has addressed issues relating to the financing of healthcare services in Iran. However, a range of opinion pieces outlined issues to be explored further in the interviews. Such issues summarised into four main categories. CONCLUSION: The health care market in Iran has faced a period in which financial issues have enhanced managerial complexity. Privatization of health care services would appear to be a step too far in assisting the system to confront its challenges at the current time. The most important step toward solving such challenges is to focus on a feasible, relevant and comprehensive policy, which optimises the use of health care resources in Iran.

The Iranian health insurance system; past experiences, present challenges and future strategies.

BACKGROUND: The Iranian healthcare system is primarily an insurance based system. This structure has an important influence on the efficiency and equity of the provision of healthcare in Iran. This paper reviews the history of the Iranian healthcare system and the impact of the Iranian health insurance system on healthcare performance based on the results of interviews with key opinion leaders and empirical evidence. METHODS: THIS REVIEW USES MIXED METHODS: a systematic literature review of electronic databases supplemented by hand searching of books and journals including Government publications and other grey literature. The issues identified were explored through a series of semi-structured interviews with key informants from within the Iranian healthcare system. The interviews were recorded transcribed, coded, classified, and analysed thematically. Empirical evidence was also sought to support or contradict the views expressed in the interviews. RESULTS: Sixteen interviews with key informants were conducted and presented anonymously. The interviewees raised many issues which were summarised into five main issues: increasing health expenditures, lack of systematic health technology assessment, very limited financial resources, challenging management and regulation, and uncovered population. CONCLUSION: A wide range of issues have affected the efficiency, quality and equity of the services provided by the Iranian healthcare system. The initial and most important step toward improving the efficiency, equity and quality of the health insurance system is to focus on evidence-based policy making to generate feasible, reasonable and comprehensive reforms.

Wheat Fusarium head blight and identification of dominant species in Moghan area, Iran.

In order to identify of head blight agents in Moghan area and determine predominant species, totally 60 samples from affected wheat heads of Atila 4, Zagros, Goadloop, Izen green and Gasquine cultivars that cultivated during 2004-2005, were collected from randomly selected commercial wheat fields in Moghan. Twenty randomly selected kernels and glumes from each sample were surface sterilized and were planted on synthesized nutrient agar medium (SNA), potato dextrose agar (PDA) and Nash-Snyder medium (NA) plates. Culture plates were incubated at 22 to 25 C with a 12-h photoperiod provided by fluorescent and ultra violet lights. For the species identification, cultures were incubated for 5 to 15 days on PDA plates to induce sporulation under light and temperature previously described. Single conidial isolates were obtained by spreading a conidial suspension across a water agar culture plate and transferring a single germinated conidium to a new PDA culture plates. Single spore cultures were grown on Carnation leaf agar (CLA) for spore morphology assessment and on PDA for color assessment. All species were identified based on descriptions given in Burgess et. al. and Nelson et al. The results indicated that in addition Fusarium graminearum and F. culmorum were identified as wheat FHB agents in Moghan area and F. graminearum was dominant species in Moghan area. Also severe infection was determined in Atila 4 cultivar by F. graminearum.

Armillaria mellea as a cause of oak decline in Hatam-baigh forest of Iran.

A200 ha forest of "Hatam-baig" is located in Ardebil Province on the Northwest of Iran. Oak trees (Quercus macranthera Fisch & Mey) in this forest have been faced with declining and extinction since 1991, that has destructed about one third of the forest trees until now. This disorder was expressed in various symptoms including wilting, defoliation and decline. In order to identify factors causing decline, a study was managed from 1998 to 2001. Samples were taken from roots, trunks, crowns and soil beneath the canopy and were cultured on different culture media subsequently. Armillaria mellea (Vahl) P. Kumm., Phytophthora cryptogea Pethybr. & Laff., Dematophora sp., Pythium aphanidermatum (Edson) Fitzp. and Fusarium spp. were the most common isolated fungi. A. mellea appeared to be the essential causal agent of the decline according to the studies made on oak tress decline around the world and based on brown rot observed beneath mycelial fans in the cross section prepared from the trunk and characteristics of the isolated fungi. The fungus activity had been favored by physiological weakness and stresses in oak rootstocks caused by brown- tail moth (Euproctis chrysorhoea L.) and drought stress in infected trees. The biological species of this fungus was identified as Armillaria mellea, using hybridization tests and application of haploid test strains. The fungi such as Phytophthora sp., Pythium sp., and Dematophora sp. can not be infective in this forest due to being hydrophylous. In the southern part of the forest with remarked steepness, the severity of the decline appears to be more than that in the smoothly northern part. The decline of Q. macranthera is reported as matrix nova. The report of the isolated fungi from this oak species is also universally new.