RESUMO
Mercury concentrations were investigated in freshwater sediment from two canals in Scotland, UK. High concentrations found in the Union Canal (35.3-1200 mg kg-1) likely originate from historical munitions manufacture, with lower levels in the Forth & Clyde Canal (0.591-9.14 mg kg-1). Concentrations of methylmercury (MeHg) were low - from 6.02 to 18.6 µg kg-1 (0.001-0.023% of total Hg) in the Union Canal and from 3.44 to 14.1 µg kg-1 (0.11-0.58% of total Hg) in the Forth & Clyde Canal - and there was a significant inverse relationship between total Hg concentration and %MeHg. Total Hg concentration was significantly negatively correlated with pH and positively correlated with Fe content (in the Union Canal only) but not with organic matter, S content or the proportion of clay present. The MeHg concentration was not correlated with any of the above sediment parameters. Ethylmercury was detected in the most highly contaminated sediments from the Union Canal.
Assuntos
Monitoramento Ambiental/métodos , Água Doce/química , Sedimentos Geológicos/química , Mercúrio/análise , Compostos de Metilmercúrio/análise , Poluentes Químicos da Água/análise , Alquilação , Mercúrio/química , Compostos de Metilmercúrio/química , Escócia , Poluentes Químicos da Água/químicaRESUMO
INTRODUCTION: Surveys that collect information on injuries often focus on the single "most serious" event to help limit recall error and reduce survey length. However, this can mask less serious injuries and result in biased incidence estimates for specific injury subcategories. METHODS: Data from the 2002 Health Behaviour in School-aged Children (HBSC) survey and from the Canadian Hospitals Injury Reporting and Prevention Program (CHIRPP) were used to compare estimates of sports injury incidence in Canadian children. RESULTS: HBSC data indicate that 6.7% of children report sustaining a sports injury that required an emergency department (ED) visit. However, details were only collected on a child's "most serious" injury, so children who had multiple injuries requiring an ED visit may have had sports injuries that went unreported. The rate of 6.7% can be seen to be an underestimate by as much as 4.3%. Corresponding CHIRPP surveillance data indicate an incidence of 9.9%. Potential masking bias is also highlighted in our analysis of injuries attended by other health care providers. CONCLUSION: The "one most serious injury" line of questioning induces potentially substantial masking bias in the estimation of sports injury incidence, which limits researchers' ability to quantify the burden of sports injury. Longer survey recall periods naturally lead to greater masking. The design of future surveys should take these issues into account. In order to accurately inform policy decisions and the direction of future research, researchers must be aware of these limitations.
INTRODUCTION: Les enquêtes sur la cueillette de données sur les blessures ciblent souvent « l'événement le plus grave ¼ dans le but de limiter les erreurs de mémoire et de réduire la longueur des questionnaires. Cela risque cependant de masquer des blessures moins graves et d'entraîner un biais dans les estimations des taux d'incidence de certaines sous-catégories de traumatismes. MÉTHODOLOGIE: Nous avons utilisé des données tirées de l'Enquête sur les comportements liés à la santé des enfants d'âge scolaire (ECSEAS) et du Système canadien hospitalier d'information et de recherche en prévention des traumatismes (SCHIRPT) pour comparer les estimations des taux d'incidence des blessures sportives chez les enfants canadiens. RÉSULTATS: D'après les données de l'ECSEAS, 6,7 % des enfants déclarent avoir subi une blessure sportive ayant nécessité une consultation à un service des urgences. N'ont cependant été recueillies que les données sur la blessure « la plus grave ¼ subie par l'enfant, ce qui fait qu'un enfant ayant subi de multiples blessures justifiant une consultation à un service des urgences est susceptible d'avoir subi des blessures sportives passées sous silence. La sous-estimation de ce taux de 6,7 % pourrait atteindre 4,3 %. D'après les données de surveillance du SCHIRPT correspondantes, le taux d'incidence de ces blessures est de 9,9 %. Nous mettons également en lumière dans notre analyse le biais potentiel de masquage lié aux blessures traitées par d'autres prestataires de soins de santé. CONCLUSION: Poser une question sur « la blessure la plus grave ¼ risque d'induire un biais de masquage considérable du taux d'incidence des blessures sportives, limitant ainsi la capacité des chercheurs à estimer l'ampleur réelle du phénomène. Des périodes de rappel plus longues entraînent inévitablement un phénomène de masquage plus important. La conception des enquêtes à venir devrait tenir compte de ces réalités. Si l'on veut faciliter une prise de décisions éclairées et orienter correctement les futures recherches, il faut que les chercheurs soient conscients de ces limitations.
Assuntos
Traumatismos em Atletas , Relatório de Pesquisa/normas , Traumatismos em Atletas/diagnóstico , Traumatismos em Atletas/epidemiologia , Viés , Canadá/epidemiologia , Criança , Serviço Hospitalar de Emergência/estatística & dados numéricos , Feminino , Inquéritos Epidemiológicos/métodos , Inquéritos Epidemiológicos/normas , Humanos , Incidência , Masculino , Índices de Gravidade do TraumaRESUMO
Surface sediments collected from the Lagos Lagoon, Nigeria, and three adjoining rivers were analysed for their physicochemical properties and pseudo-total concentration of the potentially toxic metals (PTM) Cd, Cr, Cu, Pb and Zn. The concentration of the PTM varied seasonally and spatially. Odo-Iyaalaro was observed to be the most polluted river, with highest concentrations of 42.1 mg kg(-1), 102 mg kg(-1), 185 mg kg(-1), 154 mg kg(-1) and 1040 mg kg(-1) of Cd, Cr, Cu, Pb and Zn, respectively, while Ibeshe River was the least contaminated, apart from a site affected by Cu from the textile industry. Some of the sediments were found to be above the consensus-based probable effect concentrations and Dutch sediment guideline for metals. Overall metal concentrations were similar to those reported for other tropical lagoon and estuarine systems affected by anthropogenic inputs as a result of rapid urbanisation. Due to the large number of samples, principal component analysis was used to examine relationships within the data set. Generally, sediments collected during the dry season were observed to have higher concentration of PTM than those collected during the rainy season. This means that PTM could accumulate over a prolonged period and then be released relatively rapidly, on an annual basis, into tropical lagoon systems.
Assuntos
Sedimentos Geológicos/química , Metais/análise , Poluentes Químicos da Água/análise , Poluição Química da Água/estatística & dados numéricos , Monitoramento Ambiental , Análise Multivariada , Nigéria , Análise de Componente Principal , Estações do Ano , Água do Mar/química , Indústria TêxtilRESUMO
An innovative technique for rapid identification and quantification of mercury (Hg) species in soils and sediments was developed using a direct mercury analyser. Speciation was performed by the continuous thermal-desorption of mercury species (temperature range 76-770 °C), in combination with atomic absorption spectrophotometry detection. Standard materials HgCl(2), Hg bound to humic acids and HgS were characterized; thermo-desorption curves of each material showed a well-resolved peak at specific temperature intervals: 125-225 °C, 100-250 °C and 225-325 °C, respectively. Certified reference materials (CRM) BCR(®) 142R, RTC(®) CRM 021, NRC(®) MESS-3 and PACS-2 were tested. Although the CRM were not certified for Hg species, the sum of Hg species obtained was compared to the certified value for total Hg; recoveries were 92%, 100%, 97%, and 95%, respectively. One sediment and three soil samples from mercury contaminated areas (total Hg concentrations 0.067-126 mg kg(-1)) were analysed as well. It was possible to compare peaks of thermo-desorption curves from the samples with those from standard materials and thereby distinguish different Hg species in solid samples. Generally, Hg was present as bound to chloride or humic substances. The precision was satisfactory, as reflected by the relative standard deviations determined for standards and certified reference materials (<11%; n=10).
RESUMO
Photo-degradation of tributyltin (TBT) has been enhanced by TiO(2) nanoparticles doped with nitrogen (N-doped TiO(2)). The N-doped catalyst was prepared by a sol-gel reaction of titanium (IV) tetraisopropoxide with 25% ammonia solution and calcined at various temperatures from 300 to 600°C. X-ray diffraction results showed that N-doped TiO(2) remained amorphous at 300°C. At 400°C the anatase phase occurred then transformed to the rutile phase at 600°C. The crystallite size calculated from Scherrer's equation was in the range of 16-51 nm which depended on the calcination temperature. N-doped TiO(2) calcined at 400°C which contained 0.054% nitrogen, demonstrated the highest photocatalytic degradation of TBT at 28% in 3h under natural light when compared with undoped TiO(2) and commercial photocatalyst, P25-TiO(2) which gave 14.8 and 18% conversion, respectively.
Assuntos
Luz , Fotoquímica , Titânio/química , Compostos de Trialquitina/química , Catálise , Temperatura Alta , Microscopia Eletrônica de Transmissão , Difração de Raios XRESUMO
The concentration of PCBs in topsoils from five European cities was assessed and the highest levels were found in Glasgow (Scotland), followed by Torino (Italy), Aveiro (Portugal), Ljubljana (Slovenia) and Uppsala (Sweden). All cities showed the presence of local sources in addition to diffuse contamination from global atmospheric transport. The association of general soil parameters with PCBs in Glasgow and with heavier congeners in Torino and Ljubljana indicates that retention of these compounds is occurring. The profiles obtained resemble Aroclor 1254 and 1260, which are important local sources. Nevertheless, differences in PCB profiles were observed among cities, due to the combined effects of the age of the contamination (which determines the time available for volatilisation and degradation), different sources of PCBs and differences in climate (which influence volatilisation and deposition).
Assuntos
Poluentes Ambientais/análise , Bifenilos Policlorados/análise , Solo/análise , Saúde da População Urbana/estatística & dados numéricos , Físico-Química , Cidades , Monitoramento Ambiental/métodos , Europa (Continente) , HumanosRESUMO
Soils from Aveiro, Glasgow, Ljubljana, Sevilla and Torino have been investigated in view of their potential for translocation of potentially toxic elements (PTE) to the atmosphere. Soils were partitioned into five size fractions and Cr, Cu, Ni, Pb and Zn were measured in the fractions and the whole soil. All PTE concentrated in the <10 microm fraction. Cr and Ni concentrated also in the coarse fraction, indicating a lithogenic contribution. An accumulation factor (AF) was calculated for the <2 and <10 microm fraction. The AF values indicate that the accumulation in the finer fractions is higher where the overall contamination is lower. AF for Cr and Ni are particularly low in Glasgow and Torino. An inverse relationship was found between the AF of some metals and the percentage of <10 microm particles that could be of use in risk assessment or remediation practices.
Assuntos
Monitoramento Ambiental/métodos , Resíduos Perigosos , Metais/análise , Poluentes do Solo/análise , Solo/análise , Poluentes Atmosféricos , Cromo , Cidades , Cobre/análise , Europa (Continente) , Chumbo/análise , Níquel/análise , Tamanho da Partícula , Zinco/análiseRESUMO
Corticosterone, a glucocorticoid secreted during stress responses, has a range of actions that help birds respond to stressors. Although effects of corticosterone treatment have been described in several avian species, the impacts of defined increases in plasma corticosterone on early development and on corticosterone stress responses are little known. These issues were addressed by providing quail with different doses of corticosterone in drinking water from days 8 to 38 post-hatch. The corticosterone dose consumed by each bird during treatment days 15-30 was calculated by measuring water intake. The corticosterone dose was inversely, but weakly, correlated with weights of the bursa, thymus, spleen, liver, testes, oviduct, muscle, and body, and positively correlated with peritoneal fat deposition. When birds were divided into groups based on their corticosterone intake, weights of the spleen, thymus, bursa, muscle, testes, and oviduct were significantly reduced in birds receiving the highest doses; with the exception of muscle, similar reductions were also observed in birds receiving medium doses, and thymic growth was inhibited in birds receiving low doses. The acute corticosterone stress response was measured by handling birds for 15 min. Plasma corticosterone was transiently increased at 15 min in control birds in response to the handling stressor. Some birds consuming low doses of corticosterone had corticosterone responses similar to control birds. Initial corticosterone concentrations were elevated in birds consuming higher doses of corticosterone. Plasma corticosterone in these birds decreased from 0 to 15 min, then increased from 15 to 30 min. The initial decrease could be due to corticosterone clearance, whilst the increase could indicate that the birds had a greater response than control birds to isolation as a stressor. Corticosterone treatment may have reduced the strength of corticosterone negative feedback within the hypothalamo-pituitary-adrenal axis. The results indicate that individuals and organs differ in their sensitivity to corticosterone. Moreover, elevated plasma corticosterone may disrupt the acute corticosterone stress response, and may thus reduce the ability of birds to cope with stressors.
Assuntos
Corticosterona/metabolismo , Coturnix/metabolismo , Manobra Psicológica , Estresse Psicológico/metabolismo , Envelhecimento/metabolismo , Animais , Peso Corporal , Bolsa de Fabricius/crescimento & desenvolvimento , Bolsa de Fabricius/metabolismo , Corticosterona/administração & dosagem , Corticosterona/sangue , Coturnix/sangue , Coturnix/crescimento & desenvolvimento , Relação Dose-Resposta a Droga , Ingestão de Líquidos , Feminino , Crescimento e Desenvolvimento/efeitos dos fármacos , Fígado/crescimento & desenvolvimento , Fígado/metabolismo , Masculino , Músculos/metabolismo , Tamanho do Órgão , Oviductos/crescimento & desenvolvimento , Oviductos/metabolismo , Baço/crescimento & desenvolvimento , Baço/metabolismo , Estresse Psicológico/sangue , Estresse Psicológico/fisiopatologia , Testículo/crescimento & desenvolvimento , Testículo/metabolismo , Timo/crescimento & desenvolvimento , Timo/metabolismoRESUMO
The purpose of this study was to determine the degree of contamination with polycyclic aromatic hydrocarbons (PAHs) in samples of urban soil from three European cities: Glasgow (UK), Torino (Italy) and Ljubljana (Slovenia). Fifteen PAHs (naphthalene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenzo[a,h]anthracene, benzo[g,h,i]perylene, indeno[1,2,3-c,d]pyrene) were measured in urban soil samples, using harmonised sampling, sample extraction and analyte quantification methods. Although the mean concentration of each PAH in urban soils of each city showed a wide range of values, high levels of contamination were only evident in Glasgow, where the sum of concentrations of 15 PAHs was in the range 1487-51,822 microg kg(-1), cf. ranges in the other two cities were about ten-fold lower (89.5-4488 microg kg(-1)). The three predominant PAHs were phenanthrene, fluoranthene and pyrene, with the sum of these compounds about 40% of the total PAH content. These data, together with some special molecular indices based on ratios of selected PAHs, suggest pyrogenic origins, especially motor vehicle exhausts, to be the major sources of PAHs in urban soils of the three cities. The largest concentrations for PAHs were often found in sites close to the historic quarters of the cities. Overall, the different climatic conditions, the organic carbon contents of soil, and the source apportionment were the dominant factors affecting accumulation of PAHs in soil.
Assuntos
Cidades , Hidrocarbonetos Policíclicos Aromáticos/análise , Poluentes do Solo/análise , Monitoramento Ambiental , Itália , Eslovênia , Reino UnidoRESUMO
Metals released by the extraction with aqua regia, EDTA, dilute HCl and sequential extraction (SE) by the BCR protocol were studied in urban soils of Sevilla, Torino, and Glasgow. By multivariate analysis, the amounts of Cu, Pb and Zn liberated by any method were statistically associated with one another, whereas other metals were not. The mean amounts of all metals extracted by HCl and by SE were well correlated, but SE was clearly underestimated by HCl. Individual data for Cu, Pb and Zn by both methods were correlated only if each city was considered separately. Other metals gave poorer relationships. Similar conclusions were reached comparing EDTA and HCl, with much lower values for EDTA. Dilute HCl extraction cannot thus be recommended for general use as alternative to BCR SE in urban soils.
Assuntos
Metais Pesados/química , Poluentes do Solo/química , Fenômenos Químicos , Físico-Química , Cobre/química , Ácido Edético/química , Ácido Clorídrico/química , Chumbo/química , Ácido Nítrico/química , Análise de Componente Principal , Solo/análise , Soluções/química , Urbanização , Zinco/químicaRESUMO
Use of a harmonised sampling regime has allowed comparison of concentrations of copper, chromium, nickel, lead and zinc in six urban parks located in different European cities differing markedly in their climate and industrial history. Wide concentrations ranges were found for copper, lead and zinc at most sites, but for chromium and nickel a wide range was only seen in the Italian park, where levels were also considerably greater than in other soils. As might be expected, the soils from older cities with a legacy of heavy manufacturing industry (Glasgow, Torino) were richest in potentially toxic elements (PTEs); soils from Ljubljana, Sevilla and Uppsala had intermediate metal contents, and soils from the most recently established park, in the least industrialised city (Aveiro), displayed lowest concentrations. When principal component analysis was applied to the data, associations were revealed between pH and organic carbon content; and between all five PTEs. When pH and organic carbon content were excluded from the PCA, a distinction became clear between copper, lead and zinc (the "urban" metals) on the one hand, and chromium and nickel on the other. Similar results were obtained for the surface (0-10 cm depth) and sub-surface (10-20 cm depth) samples. Comparisons with target or limit concentrations were limited by the existence of different legislation in different countries and the fact that few guidelines deal specifically with public-access urban soils intended for recreational use.
Assuntos
Cidades/legislação & jurisprudência , Monitoramento Ambiental/normas , Resíduos Industriais/análise , Metais Pesados/análise , Poluentes do Solo/análise , Europa (Continente) , Metais Pesados/toxicidade , Poluentes do Solo/toxicidadeRESUMO
The objective of this study was to quantify and assess for the first time the variability of total mercury in urban soils at a European level, using a systematic sampling strategy and a common methodology. We report results from a comparison between soil samples from Aveiro (Portugal), Glasgow (Scotland), Ljubljana (Slovenia), Sevilla (Spain), Torino (Italy) and Uppsala (Sweden). At least 25 sampling points (in about 4-5 ha) from a park in each city were sampled at two depths (0-10 and 10-20 cm). Total mercury was determined by pyrolysis atomic absorption spectrometry with gold amalgamation. The quality of results was monitored using certified reference materials (BCR 142R and BCR 141R). Measured total mercury contents varied from 0.015 to 6.3 mg kg(-1). The lowest median values were found in Aveiro, for both surface (0-10 cm) and sub-surface (10-20 cm) samples (0.055 and 0.054 mg kg(-1), respectively). The highest median mercury contents in soil samples were found in samples from Glasgow (1.2 and 1.3 mg kg(-1), for surface and sub-surface samples, respectively). High variability of mercury concentrations was observed, both within each park and between cities. This variability reflecting contributions from natural background, previous anthropogenic activities and differences in the ages of cities and land use, local environmental conditions as well as the influence of their location within the urban area. Short-range variability of mercury concentrations was found to be up to an order of magnitude over the distance of only a few 10 m.
Assuntos
Mercúrio/análise , Poluentes do Solo/análise , Cidades , Monitoramento Ambiental , Europa (Continente)RESUMO
Modern analytical techniques have been applied to investigate the nature of lead pipe corrosion products formed in pH adjusted, orthophosphate-treated, low alkalinity water, under supply conditions. Depth profiling and surface analysis have been carried out on pipe samples obtained from the water distribution system in Glasgow, Scotland, UK. X-ray diffraction spectrometry identified basic lead carbonate, lead oxide and lead phosphate as the principal components. Scanning electron microscopy/energy-dispersive x-ray spectrometry revealed the crystalline structure within the corrosion product and also showed spatial correlations existed between calcium, iron, lead, oxygen and phosphorus. Elemental profiling, conducted by means of secondary ion mass spectrometry (SIMS) and secondary neutrals mass spectrometry (SNMS) indicated that the corrosion product was not uniform with depth. However, no clear stratification was apparent. Indeed, counts obtained for carbonate, phosphate and oxide were well correlated within the depth range probed by SIMS. SNMS showed relationships existed between carbon, calcium, iron, and phosphorus within the bulk of the scale, as well as at the surface. SIMS imaging confirmed the relationship between calcium and lead and suggested there might also be an association between chloride and phosphorus.
Assuntos
Chumbo/química , Modelos Teóricos , Abastecimento de Água , Corrosão , Monitoramento Ambiental , Concentração de Íons de Hidrogênio , Espectrometria de Massas , Teste de Materiais , Microscopia Eletrônica de Varredura , Água/químicaRESUMO
Nerve agents are a class of organophosphorus chemicals that inhibit certain cholinesterase enzymes (ChE). If untreated, percutaneous exposure to nerve agents, such as VX (O-ethyl-S-[2(diisopropylamino)ethyl] methylphosphonothioate) can cause paralysis, apnoea and death. Much of the information concerning the percutaneous absorption and subsequent toxicity of nerve agents has been obtained using various rodent models. However, the most relevant 'skin model' is arguably the pig. Therefore, the purpose of this study was to examine the clinical manifestations of VX intoxication in the domestic white pig following a 2 LD50 (120 microg/kg) percutaneous challenge. There was a consistent onset of signs (where present) in each animal: mastication was followed by miosis, salivation, fasciculations and apnoea. Whilst ChE activity did not correlate with the onset of signs, there was a qualitative relationship in that mastication preceded substantial ChE inhibition, miosis lagged behind the linear decrease in acetylcholinesterase (AChE) activity and fasciculations and apnoea occurred after maximum ChE inhibition had been attained (5-10% of normal). These observations may be of use for the triage of patients exposed to VX. In comparison with similar studies with GD, VX did not affect glucose utilization. However, VX was similar to GD in that it caused a mild hyperkalaemia and hyperphosphataemia, although the significance of this observation was not clear. There was substantial lateral diffusion of the initial droplet of VX over the application site, indicating that, when decontaminating exposed skin, attention should also be directed to areas peripheral to the original site of exposure.
Assuntos
Administração Cutânea , Compostos Organotiofosforados/sangue , Compostos Organotiofosforados/intoxicação , Absorção Cutânea/efeitos dos fármacos , Pele/efeitos dos fármacos , Acetilcolinesterase/sangue , Animais , Exposição Ambiental , Radioisótopos , Absorção Cutânea/fisiologia , Soluções , Suínos , Fatores de Tempo , Testes de Toxicidade/métodosRESUMO
An ultrasonic bath and an ultrasonic probe have been used to develop rapid versions of the three-stage Community Bureau of Reference (BCR, now the Standards, Measurement and Testing Programme) sequential extraction procedure. The effect of the ultrasonic treatments on the extraction of copper, iron, manganese and zinc from a sewage sludge-amended soil has been assessed. Recoveries similar to those of conventional shaking (i.e., conventional value, +/- 30%) could generally be obtained for copper, manganese and zinc, but not for the important matrix element iron. With the use of compromise sonication conditions, steps 1, 2 and 3 of the sequential extraction (excluding the hydrogen peroxide digestion in step 3, which was not performed with sonication) could be completed in 3, 5 and 1 min, respectively, using the ultrasonic probe, and in 3, 1 and 1 h, respectively, using the bath. The extraction procedures developed using the soil performed well when applied to lake sediment BCR CRM 601. Analyte partitioning was generally similar to that obtained with mechanical shaking, and overall metal recoveries were 84-98% of those obtained with the conventional BCR protocol, except for copper extracted with the probe (74%). Poorer performance (analyte recoveries, 58-104%) was obtained when the methods were applied to an intertidal sediment. This highlights the difficulty of developing a version of the BCR extraction, with ultrasonic assistance, which gives a performance equivalent to conventional shaking when applied to different substrates.
Assuntos
Metais Pesados/química , Esgotos/química , Poluentes do Solo/análise , Sonicação , Cobre/química , Monitoramento Ambiental/métodos , Humanos , Ferro/química , Manganês/química , Zinco/químicaRESUMO
In the present study, we sought to determine whether low-grade, chronic vascular insufficiency induced in a rodent model of chronic cerebrohypoperfusion is sufficient, in and of itself, to trigger cleavage of the amyloid precursor protein (APP) into beta A-sized fragments. We report that chronic two vessel occlusion (2VO) results in progressive accumulation of beta A peptides detected by Western analysis in aged rats correlating with a shift in the immunohistochemical localization of APP from neurons to extracellular deposits in brain parenchyma. These data indicate that the 2VO paradigm reproduces features of beta A biogenesis characteristic of sporadic Alzheimer's disease.
Assuntos
Precursor de Proteína beta-Amiloide/metabolismo , Isquemia Encefálica/metabolismo , Sequência de Aminoácidos , Animais , Western Blotting , Artéria Carótida Primitiva/fisiologia , Doença Crônica , Espaço Extracelular/metabolismo , Imuno-Histoquímica , Ligadura , Masculino , Dados de Sequência Molecular , Ratos , Ratos Sprague-DawleyRESUMO
Adult rats underwent permanent bilateral occlusion of the common carotid arteries (2VO) to determine the effect of chronic cerebral ischemia on vision and retina. They were monitored post-surgically for the presence of the pupillary reflex to light. Some rats were tested for 6 months post-surgically on a radial arm maze task and then tested in another water-escape task which explicitly tested visual function. Another group of rats were tested post-surgically for 3 months on a task which simultaneously assessed visual and tactile discrimination ability. The thicknesses of the retinal sub-layers were then measured for some rats. Fourteen of the 25 rats that underwent 2VO lost the pupillary reflex. This seemed to occur within 5 days. Rats that lost the pupillary reflex but not rats whose reflex was intact, were impaired on all visually guided mazes. Tactile discrimination ability was unaffected. Only rats that lost the pupillary reflex showed reduced thickness of the retinal outer nuclear and plexiform layers, reduced cell density in the retinal ganglion cell layer and astrocytosis and degeneration of the optic tract. We conclude that 2VO can eliminate the pupillary reflex. Photoreceptors and retinal ganglion cells degenerate, but it is unclear if these are the cause(s) or result(s) of the loss of the pupillary reflex. These effects are accompanied by impairment of visually guided behavior. The possibility that visual system damage may also occur in acute ischemia merits further investigation.
Assuntos
Isquemia Encefálica/fisiopatologia , Circulação Cerebrovascular/fisiologia , Doenças Neurodegenerativas/etiologia , Doenças Neurodegenerativas/fisiopatologia , Reflexo Pupilar/fisiologia , Retina/fisiopatologia , Doenças Retinianas/etiologia , Doenças Retinianas/fisiopatologia , Transtornos da Visão/etiologia , Transtornos da Visão/fisiopatologia , Animais , Comportamento Animal/fisiologia , Artérias Carótidas/fisiopatologia , Modelos Animais de Doenças , Masculino , Nervo Óptico/irrigação sanguínea , Nervo Óptico/fisiopatologia , Ratos , Ratos Sprague-Dawley , Acidente Vascular Cerebral/fisiopatologiaRESUMO
The asymmetric segregation of cell-fate determinants and the generation of daughter cells of different sizes rely on the correct orientation and position of the mitotic spindle. In the Drosophila embryo, the determinant Prospero is localized basally and is segregated equally to daughters of similar cell size during epidermal cell division. In contrast, during neuroblast division Prospero is segregated asymmetrically to the smaller daughter cell. This simple switch between symmetric and asymmetric segregation is achieved by changing the orientation of cell division: neural cells divide in a plane perpendicular to that of epidermoblast division. Here, by labelling mitotic spindles in living Drosophila embryos, we show that neuroblast spindles are initially formed in the same axis as epidermal cells, but rotate before cell division. We find that daughter cells of different sizes arise because the spindle itself becomes asymmetric at anaphase: apical microtubules elongate, basal microtubules shorten, and the midbody moves basally until it is positioned asymmetrically between the two spindle poles. This observation contradicts the widely held hypothesis that the cleavage furrow is always placed midway between the two centrosomes.
Assuntos
Drosophila/crescimento & desenvolvimento , Neurônios/citologia , Fuso Acromático/fisiologia , Anáfase/fisiologia , Animais , Drosophila/citologia , Células Epidérmicas , Epiderme/crescimento & desenvolvimento , Histonas/análise , Histonas/metabolismo , Interfase/fisiologia , Metáfase/fisiologia , Microscopia Confocal , Sistema Nervoso/citologia , Sistema Nervoso/crescimento & desenvolvimento , Neurônios/fisiologia , Fosforilação , Prófase/fisiologia , Rotação , Fuso Acromático/química , Células-Tronco/citologia , Células-Tronco/fisiologia , Tubulina (Proteína)/análiseRESUMO
This paper provides additional data on a sewage sludge amended soil certified reference material, CRM 483, which was certified in 1997 for its EDTA and acetic acid extractable contents of some trace metals, following standardised extraction procedures. The additional work aimed to test the long-term stability of the material and the applicability of an improved version of the BCR three-step sequential extraction procedure on the sewage sludge amended soil (CRM 483). The paper demonstrates the CRM 483 long-term stability for EDTA and acetic acid extractable contents of Cd, Cr, Cu, Ni, Pb and Zn and gives the results (obtained in the framework of an interlaboratory study) for the extractable contents of the same elements in the CRM 483, following the BCR three-step sequential extraction scheme. The aqua regia extractable contents following the ISO 11466 Standard are also given. The data are given as indicative (not certified) values.
Assuntos
Monitoramento Ambiental/métodos , Metais Pesados/análise , Poluentes do Solo/análise , Ácido Acético/química , Quelantes/química , Técnicas de Química Analítica/métodos , Ácido Edético/química , Indicadores e Reagentes/química , Valores de Referência , Esgotos/químicaRESUMO
Made-up ground collected from layers of a trial pit excavated on a former industrial site was treated with artificial rainwater in a series of column leaching and sorption experiments. Metal mobility and the ability of various layers of material obtained from the pit to act as sources or sinks of potentially toxic elements were assessed. Samples from different layers varied in their abilities to raise the pH of rainwater applied at pH 3.5 and 4.3, and this was reflected in the amounts of metals mobilised by the rainwater as it percolated through the soil column. Material from the top two layers of the pit released cadmium, copper, manganese, lead, nickel and zinc to the aqueous phase, but the lower layers, with higher buffering capacity, were able to resist acidification even when the equivalent of 12 months' rainfall (western UK) was applied. Column sorption experiments confirmed the ability of material from layer 4 (48-50 cm) to take up copper, manganese and zinc. Metals were determined in the leachates by flame and electrothermal atomic absorption spectrometry and principle anions by ion chromatography.