Chemical composition and effect of essential oils of Thymus vulgaris and Origanum vulgare on adults and ovigerous masses of Pseudosuccinea columella.

Fasciolosis is a worldwide distribution zoonosis that causes great damage in ruminant breeding and has the aquatic mollusc Pseudosuccinea columella as an intermediate host. Synthetic molluscicides are the most used for control; however, they are harmful to fauna and flora. Therefore, this study aimed to evaluate the effect of essential oils from Thymus vulgaris, Origanum vulgare, and terpene carvacrol, on adult molluscs and eggs of P. columella. Analysis of EO volatile components was carried out on a gas chromatograph equipment coupled with mass spectrometry selective detector. The studied components were diluted in concentrations of 10, 20, 40, 60, 80 and 100 ppm, and it was observed that O. vulgare at concentrations of 60, 80 and 100 ppm, carvacrol at the concentrations of 80 and 100 ppm, and T. vulgaris at a concentration of 80 ppm led to 100% mortality of molluscs. All concentrations the substances tested showed 100% ovicidal activity.

[Sensibility and reproducibility of a sedimental technique (Foreyt, 2005) for Fasciola hepatica diagnosis]. / Sensibilidade e reprodutibilidade da técnica de sedimentação (Foreyt, 2005) para o diagnóstico de Fasciola hepatica.

The purposes of this study were to evaluate the sensibility and reproducibility of Foreyt (2005) technique in the diagnosis of Fasciola hepatica. A total of 686 bovines were examined at a slaughtered house and 97 were positive for Fasciola hepatica. Feces samples were collected and 58 were positive for the Foreyt technique. The validity was tested by the sensibility (59.8%) and specificity (100%). The reproductability, calculated by the Kappa agreement was 0.70 and the Chi-square showed differences between the tests (p < 0.001).