Defining consensus leukemia-associated immunophenotypes for detection of minimal residual disease in acute myeloid leukemia in a multicenter setting.

Flow-cytometric detection of minimal residual disease (MRD) has proven in several single-institute studies to have an independent prognostic impact. We studied whether this relatively complex approach could be performed in a multicenter clinical setting. Five centers developed common protocols to accurately define leukemia-associated (immuno)phenotypes (LAPs) at diagnosis required to establish MRD during/after treatment. List mode data files were exchanged, and LAPs were designed by each center. One center, with extensive MRD experience, served as the reference center and coordinator. In quarterly meetings, consensus LAPs were defined, with the performance of centers compared with these. In a learning (29 patients) and a test phase (35 patients), a mean of 2.2 aberrancies/patient was detected, and only 1/63 patients (1.6%) had no consensus LAP(s). For the four centers without (extensive) MRD experience, clear improvement could be shown: in the learning phase, 39-63% of all consensus LAPs were missed, resulting in a median 30% of patients (range 21-33%) for whom no consensus LAP was reported; in the test phase, 27-40% missed consensus LAPs, resulting in a median 16% (range 7-18%) of 'missed' patients. The quality of LAPs was extensively described. Immunophenotypic MRD assessment in its current setting needs extensive experience and should be limited to experienced centers.

Popliteal cysts in children: a retrospective study of 62 cases.

Popliteal cysts in children are usually asymptomatic and are usually found fortuitously by the parents. This study presents a series of 62 children with popliteal cysts. Among the 51 patients who received no treatment, the cysts had already disappeared at the time of the study in 43 patients; the remaining 8 patients are under 14 years of age and are without complaints. This study confirms the hypothesis that the primary popliteal cyst in children usually disappears before the age of 18. Surgery presents unnecessary risks for the patient ; moreover, the chance of recurrence after surgery is real.

Malignant hematologic disorders in two Robertsonian 13;14 translocation carriers.

Two patients with a malignant hematologic disorder appeared to carry a constitutional Robertsonian 13;14 translocation. The presence of this translocation in relation to hematologic diseases is discussed. No conclusion can be made at present on the observed association. The translocation might predispose to malignant transformation, but its presence might also be purely coincidental.