RESUMO
This study aimed to investigate the effect of selenium and magnesium nanoparticles and their combination on growth, serum biochemical and immune parameters, serum hepatic enzymes, and digestive enzymes in Asian Sea bass (Lates calcarifer), with an average weight of 32.78 ± 1.16 g for 42 days. After 4 weeks of adaptation to experimental conditions, 96 fish were randomly distributed equally in 12 cylindrical 300-l fiberglass tanks. Four treatment groups included control, 4 mg nano-selenium (Nano-Se), 500 mg nano-magnesium (Nano-Mg), and a combined treatment of 4 mg Nano-SE + 500 mg Nano-Mg treatments examined in this study. The fish fed twice a day as satiation. The results showed fish fed with combined treatment showed the highest rate of body weight gain and the specific growth rate. Digestive enzymes were significantly different between experimental treatments (p < 0.05), whereas amylase did not show a significant difference between experimental treatments (p > 0.05). The results of this study showed that serum levels of glucose, cholesterol, triglyceride, and protein indices did not show any significant difference (p > 0.05). The immunoglobulin, IgM, C3, and ACH50 indexes did not differ significantly between the experimental treatments (p > 0.05). But cortisol and lysozyme were significantly different between the experimental treatments (p < 0.05). Serum levels of SGPT, SGOT, ALP, and LDH decreased in comparison with the control group (p < 0.05). The results of the present study showed that adding Nano-Se and Nano-Mg to the diet of L. calcarifer had a positive effect on the growth and non-specific immune system with no disturbance in serum biochemical and hepatic enzymes parameters.
