Amphiphilic polyphenol incorporated hydrogel derived from mucoadhesive of Dillenia indica: Potential antioxidant and adsorbent.

In this work, the mucoadhesive substances from the fruits and seeds of Dillenia indica (DI), a plant present abundantly in India, have been extracted and utilised to prepare a hydrogel. A synthetically prepared amphiphilic polyphenol (L) has been incorporated within the hydrogel network to enhance the hydrogelation property. Moreover, the DI-L hydrogel's total phenolic content and radical scavenging prospects have been investigated. The DI-L hydrogel has shown good, sensitive, and efficient adsorptive removal of Fe(III) from the aqueous medium with an adsorption capacity of 6.157 mg/g for an initial concentration of 10 mg/L of Fe(III) solution. As a result, these findings elucidate the most innovative application of transforming fruit mucoadhesive into sustainable environmental solutions.

Quinoxaline-probe embedded injectable fluorogenic hydrogels: Comparative detection of mesitylene in guar gum and i-carrageenan hydrogels.

The innovation of novel chemosensor probes for the recognition of trace volatile organic compounds is critical due to their hazardous effect on the environment and human health. A nitro-group integrated quinoxaline probe with a profound discriminative fluorescence 'turn-on' response to mesitylene was fabricated into guar gum and i-carrageenan, two biopolymer-based hydrogel matrices, to develop compact, portable fluorogenic hydrogel sensors and assess their fluorescence properties. A comparative characterization-based analysis of native, probe-associated, and probe-analyte-associated hydrogels, (comprising of FT-IR, XRD, TGA) was investigated to ascertain the overall compatibility of the hydrogel-based sensors for use as a smart rapid detection tool. Dynamic rheological measurements also validated the mechanical stability and robustness of the developed hydrogel matrices. Fluorescence spectroscopic investigations yielded promising results of 0.15 ppm limit of detection (LOD) in guar gum and 0.29 ppm LOD in i-carrageenan hydrogels respectively. FESEM and Fluorescence microscopy studies represented the morphological variations of the hydrogel sensors on interaction with mesitylene. The practical feasibility of the chemosensor in hydrogel form for mesitylene detection in the vapor phase was also explored. Probe-embedded hydrogels with injectable property was shown, depicting its use as security ink for information encryption functions. This approach of incorporating chemosensors into biobased hydrogel networks has the potential to broaden its opportunities in the field of chemical, biomedical, and environmental sensing sectors.

Regulation of volatile fatty acid accumulation from waste: Effect of inoculum pretreatment.

The study investigates the implications of waste feedstock, inoculum origin, and pretreatment on volatile fatty acids accumulation (VFA). The acidogenic fermentation of the feedstocks, rice mill effluent (RME), and brewery effluent (BE) was studied using untreated and pretreated (cyclic heat-acid shock) brewery anaerobic sludge as inoculum. The pretreatment was successful in refining and stabilizing VFA production from the feedstocks. The fermentation of RME with pretreated sludge had an enhanced acetate yield of 0.37 ± 0.02 mgCOD/mgCOD, even to odd ratio of 20.97 ± 0.08 mg/mg and the highest butyrate yield of 0.39 ± 0.01 mgCOD/mgCOD compared to untreated system. The pretreated system had stability in COD and pH profile, while VFA content depends on the origin of inoculum. Pretreatment inhibited the carbon sinks and augmented acetate-butyrate type metabolism with stable performance. The fermentation of RME by pretreated sludge produced a higher even-numbered VFAs and enhanced even to odd ratio in comparison with fermentation of BE, thereby affecting polymer composition and property. PRACTITIONER POINTS: The pretreated system had stable acidification, chemical oxygen demand, and pH profile. The pretreated system had higher acetate and butyrate yield compared to the untreated system. Rice mill effluent acidified with pretreated sludge had the highest even to odd ratio, 20.97 mg/mg. The even to odd ratio for acidification of brewery effluent was insignificant. Pretreatment, the origin of sludge, and the effluent had a regulatory effect on acidification.

Influence of inoculum variation and nutrient availability on polyhydroxybutyrate production from activated sludge.

Carbon recovery through polyhydroxybutyrate (PHB) production can create a value-added waste management system. Activated sludge as inoculum enables PHB production using cheap and renewable carbons source, bringing PHB at par to conventional plastics. The PHB accumulating potential of activated sludge needs to be improved to realize the objective. The interaction between the origin of activated sludge, petroleum refinery sludge and brewery sludge, and nitrogen availability was explored to effect culture enrichment, improve PHB accumulation, and polymer characteristics through aerobic dynamic feeding. Consequently, nitrogen excess and limitation enrichment of both sludges produced mix microbial culture with adequate PHB storage of 7.8 ± 0.05%, 14.4 ± 0.04%, 14.4 ± 0.04%, 13.4 ± 0.02% respectively. Batch accumulation revealed higher PHB accumulation of 76.1 ± 0.03% and 71.7 ± 0.05% under nitrogen limitation for PRS and BS enriched under nitrogen excess condition compared to any other combination. The higher decomposition temperature of 285 °C, 293 °C, and a lower melting point of 168 °C, 165 °C with a higher molecular weight of 4.3x105g/mol and semi-crystalline arrangement indicates the potential applications for extracted PHB. PHB production enhanced under nitrogen limited conditions with culture enriched under nitrogen excess condition. However, similar PHB storage, physiochemical property, and overlapping microbial community show an insignificant effect of sludge origin on PHB production.

Bioethanol production from leafy biomass of mango (Mangifera indica) involving naturally isolated and recombinant enzymes.

The present study describes the usage of dried leafy biomass of mango (Mangifera indica) containing 26.3% (w/w) cellulose, 54.4% (w/w) hemicellulose, and 16.9% (w/w) lignin, as a substrate for bioethanol production from Zymomonas mobilis and Candida shehatae. The substrate was subjected to two different pretreatment strategies, namely, wet oxidation and an organosolv process. An ethanol concentration (1.21 g/L) was obtained with Z. mobilis in a shake-flask simultaneous saccharification and fermentation (SSF) trial using 1% (w/v) wet oxidation pretreated mango leaves along with mixed enzymatic consortium of Bacillus subtilis cellulase and recombinant hemicellulase (GH43), whereas C. shehatae gave a slightly higher (8%) ethanol titer of 1.31 g/L. Employing 1% (w/v) organosolv pretreated mango leaves and using Z. mobilis and C. shehatae separately in the SSF, the ethanol titers of 1.33 g/L and 1.52 g/L, respectively, were obtained. The SSF experiments performed with 5% (w/v) organosolv-pretreated substrate along with C. shehatae as fermentative organism gave a significantly enhanced ethanol titer value of 8.11 g/L using the shake flask and 12.33 g/L at the bioreactor level. From the bioreactor, 94.4% (v/v) ethanol was recovered by rotary evaporator with 21% purification efficiency.

Purification and characterization of an alkaline cellulase produced by Bacillus subtilis (AS3).

An extracellular alkaline carboxymethycellulase (CMCase) from Bacillus subtilis was purified by salt precipitation followed by anion-exchange chromatography using DEAE-Sepharose. The cell-free supernatant containing crude enzyme had a CMCase activity of 0.34 U/mg. The purified enzyme gave a specific activity of 3.33 U/mg, with 10-fold purification and an overall activity yield of 5.6%. The purified enzyme displayed a protein band on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with an apparent molecular size of 30 kDa, which was also confirmed by zymogram analysis. The enzyme displayed multisubstrate specificity, showing significantly higher activity with lichenan and ß-glucan as compared to carboxymethylcellulose (CMC), laminarin, hydroxyethylcellulose, and steam-exploded bagasse, and negligible activity with crystalline substrate such as Avicel and filter paper. It was optimally active at pH 9.2 and temperature 45°C. The enzyme was stable in the pH range 6-10 and retained 70% activity at pH 12. Thermal stability analysis revealed that the enzyme was stable in temperature range of 20°C to 45°C and retained more than 50% activity at 60°C for 30 min. The enzyme had a Km of 0.13 mg/ml and Vmax of 3.38 U/mg using CMC as substrate.

Enhanced Cellulase Production from Bacillus subtilis by Optimizing Physical Parameters for Bioethanol Production.

Effect of physical parameters such as initial pH, agitation (rpm), and temperature (°C) for cellulase production from Bacillus subtilis AS3 was investigated. Central composite design of experiments followed by multiple desirability function was applied for the optimization of cellulase activity and cell growth. The effect of the temperature and agitation was found to be significant among the three independent variables. The optimum levels of initial pH, temperature, and agitation for alkaline carboxymethylcellulase (CMCase) production predicted by the model were 7.2, 39°C, and 121 rpm, respectively. The CMCase activity with unoptimized physical parameters and previously optimized medium composition was 0.43 U/mL. The maximum activity (0.56 U/mL) and cell growth (2.01 mg/mL) predicted by the model were in consensus with values (0.57 U/mL, 2.1 mg/mL) obtained using optimized medium and optimal values of physical parameters. After optimization, 33% enhancement in CMCase activity (0.57 U/mL) was recorded. On scale-up of cellulase production process in bioreactor with all the optimized conditions, an activity of 0.75 U/mL was achieved. Consequently, the bacterial cellulase employed for bioethanol production expending (5%, w/v) NaOH-pretreated wild grass with Zymomonas mobilis yielded an utmost ethanol titre of 7.56 g/L and 11.65 g/L at shake flask and bioreactor level, respectively.

Enhancement of Cellulase Activity from a New Strain of Bacillus subtilis by Medium Optimization and Analysis with Various Cellulosic Substrates.

The cellulase activity of Bacillus subtilis AS3 was enhanced by optimizing the medium composition by statistical methods. The enzyme activity with unoptimised medium with carboxymethylcellulose (CMC) was 0.07 U/mL and that was significantly enhanced by CMC, peptone, and yeast extract using Placket-Burman design. The combined effects of these nutrients on cellulase activity were studied using 2(2) full factorial central composite design. The optimal levels of medium components determined were CMC (1.8%), peptone (0.8%), and yeast extract (0.479%). The maximum enzyme activity predicted by the model was 0.49 U/mL which was in good agreement with the experimental value 0.43 U/mL showing 6-fold increase as compared to unoptimised medium. The enzyme showed multisubstrate specificity, showing significantly higher activity with lichenan and ß-glucan and lower activity with laminarin, hydroxyethylcellulose, and steam exploded bagasse. The optimised medium with lichenan or ß-glucan showed 2.5- or 2.8-fold higher activity, respectively, at same concentration as of CMC.