RESUMO
Additional information as to the objectives of our previous paper entitled 'ERETIC implemented in diffusion-ordered NMR as a diffusion reference' (Magn. Reson. Chem. 2008, 46, S63) is provided. The need for an optimal instrumental stability for the method proposed to be reliably applicable in view of these objectives is emphasized and illustrated.
RESUMO
(1)H NMR spectroscopy was used as a method for quantitative analysis of sesquiterpene lactones present in a crude lactone fraction isolated from Arnica montana. Eight main components - tigloyl-, methacryloyl-, isobutyryl- and 2-methylbutyryl-esters of helenalin (H) and 11α,13-dihydrohelenalin (DH) were identified in the studied sample. The method allows the determination of the total amount of sesquiterpene lactones and the quantity of both type helenalin and 11α,13-dihydrohelenalin esters separately. Furthermore, 6-O-tigloylhelenalin (HT, 1), 6-O-methacryloylhelenalin (HM, 2), 6-O-tigloyl-11α,13-dihydrohelenalin (DHT, 5), and 6-O-methacryloyl-11α,13-dihydrohelenalin (DHM, 6) were quantified as individual components.
Assuntos
Arnica/metabolismo , Lactonas/análise , Espectroscopia de Ressonância Magnética/métodos , Extratos Vegetais/metabolismo , Sesquiterpenos/química , Técnicas de Química Analítica , Química Farmacêutica/métodos , Desenho de Fármacos , Flores/química , Lactonas/química , Modelos Químicos , Sesquiterpenos/análise , Sesquiterpenos de GuaianoRESUMO
A new secoberbine alkaloid (-)-N-methylcorydalisol was isolated from the aerial parts of Hypecoum lactiflorum Kar. et Kir. Pazij. (Papaveraceae) of Mongolian origin and was characterised. The known alkaloids of protopine and protoberberine type protopine, allocryptopine, (-)-N-methylcanadine and (-)-N-methylstylopine were also isolated. (-)-N-methylstylopine is a new alkaloid for the genus, while (-)-N-methylcanadine is new for the species. All structures were established by physical and spectral analysis.
Assuntos
Alcaloides/isolamento & purificação , Alcaloides de Berberina/isolamento & purificação , Ecossistema , Papaveraceae/química , Alcaloides/química , Benzofenantridinas/química , Benzofenantridinas/isolamento & purificação , Alcaloides de Berberina/química , Estrutura Molecular , MongóliaRESUMO
Mixed micelles formed from nonionic surfactant Triton X-100 (TX100), anionic surfactant sodium dodecyl dioxyethylene sulfate (SDP2S), and triblock copolymer Synperonic L61 (SL61) were investigated by 1H NMR spectroscopy. The size and shape of the aggregates were determined by diffusion ordered NMR spectroscopy (DOSY), while 2D nuclear Overhauser enhanced spectroscopy (NOESY) NMR was used to study the mutual spatial arrangement of the surfactant molecules in the aggregated state. An average micellar hydrodynamic radius of 3.6 nm, slightly increasing upon increasing TX100 molar fraction, was found for the mixed systems without additives. Addition of SL61 to the mixed micellar systems results in a slight increase of micellar radii. In the presence of AlCl3, an increase of TX100/SDP2S micellar sizes from 4 to 10 nm was found when increasing the SDP2S molar fraction. The mixed TX100/SDP2S micelles in the presence of both AlCl3 and polymer SL61 are almost spherical, with a radius of 4.5 nm. 2D NOESY data reveal that, as the individual TX100 micelles, mixed TX100/SDP2S and TX100/SDP2S/SL61/AlCl3 micelles also have a multilayer structure, with partially overlapping internal and external layers of TX100 molecules. In these mixed micelles, the SDP2S molecules are located at the level of the external layer of TX100 molecules, whereas the SL61 polymer is partially incorporated inside of the micellar core.
Assuntos
Difusão , Micelas , Octoxinol/química , Poloxâmero/química , Dodecilsulfato de Sódio/análogos & derivados , Cloreto de Alumínio , Compostos de Alumínio/química , Cloretos/química , Eletrólitos/química , Interações Hidrofóbicas e Hidrofílicas , Espectroscopia de Ressonância Magnética , Dodecilsulfato de Sódio/química , Soluções , Tensoativos/químicaRESUMO
Novel derivatives of 4,5-substituted-1,2,4-triazole-thiones and 2,5-substituted-1,3,4-thiadiazoles were synthesized and evaluated for their cytotoxicity. The biological study indicated that compounds 4-ethyl-5-(4,5,6,7-tetrahydro-1-benzothien-2-yl)-2,4-dihydro-3H-1,2,4-triazole-3-thione 13, N-ethyl-5-(4,5,6,7-tetrahydro-1-benzothien-2-yl)-1,3,4-thiadiazol-2-amine 16, 4-amino-5-(4,5,6,7-tetrahydro-1-benzothien-2-yl)-2,4-dihydro-3H-1,2,4-triazole-3-thione 20 and 4-amino-5-(5-phenylthien-2-yl)-2,4-dihydro-3H-1,2,4-triazole-3-thione 21 possessed high cytotoxicity in vitro against thymocytes. The corresponding IC(50) values were 0.46 microM, 5.2 x 10(-6)microM, 0.012 microM and 1.0 x 10(-6)microM. Most toxic against lymphocytes was compound 21, IC(50) - 0.012 microM. The tested compounds showed a general stimulation effect on B-cells' response.
Assuntos
Sistema Imunitário/efeitos dos fármacos , Tiadiazóis/síntese química , Triazóis/síntese química , Linfócitos B/efeitos dos fármacos , Humanos , Sistema Imunitário/citologia , Concentração Inibidora 50 , Linfócitos/efeitos dos fármacos , Relação Estrutura-Atividade , Tiadiazóis/farmacologia , Glândula Tireoide/citologia , Glândula Tireoide/efeitos dos fármacos , Triazóis/farmacologiaRESUMO
The ERETIC (Electronic Reference To access In vivo Concentrations) technique generates an electronic signal in the NMR spectrometer which is detected simultaneously to the sample FID during the acquisition. The implementation of the ERETIC sequence in any 2D DOSY experimental scheme enables one to generate directly into the raw 2D DOSY spectrum a reference signal with an attenuation simulated to describe a well-defined diffusion behavior. This simulated intensity attenuation can be used to evaluate the output generated by any DOSY data treatment algorithm, in a single as well as multichannel approach and provide insight into their precision, accuracy, scope and limitations. The ERETIC sequence implemented in the standard bipolar pulsed field gradient longitudinal eddy current delay (LED) sequence is illustrated on various algorithms presented previously in the literature for the analysis of the generated ERETIC-DOSY spectra of simulated model systems representing discrete and continuous diffusion profiles in mono- and bi-Gaussian diffusion regimes.
Assuntos
Difusão , Espectroscopia de Ressonância Magnética/instrumentação , Algoritmos , Espectroscopia de Ressonância Magnética/métodos , Espectroscopia de Ressonância Magnética/normas , Distribuição NormalRESUMO
The self-aggregation and supramolecular micellar structure of two surfactants in aqueous solution, the anionic surfactant SDP2S (sodium dodecyl dioxyethylene-2 sulfate) and the nonionic surfactant Triton X-100 (octylphenol-polyoxyethylene ether with 9.5 ethoxy groups), were investigated by NMR spectroscopy. The critical micellar concentration (CMC), the size, and shape of the aggregates were determined by diffusion ordered NMR spectroscopy (DOSY), while 2D NOESY NMR spectra were used to study the mutual spatial arrangement of surfactant molecules in the aggregated state. A nonlinear increase of the micellar hydrodynamic radius, indicating possible sphere-to-rod shape transition, was found for SDP2S at higher surfactant concentrations. Triton X-100 micelles were found to be almost spherical at low surfactant concentrations, but formation of ellipsoid shaped particles and/or micellar aggregation was observed at higher concentrations. The NOESY data show that at low concentration Triton X-100 forms a two-layer spherical structure in the micelles, with partially overlapping internal and external layers of Triton X-100 molecules and no distinct hydrophilic-hydrophobic boundary.
RESUMO
The static and dynamic stereochemistry of dimesityl-2,4,6-trimethoxyphenylmethane in solution was investigated by lineshape analysis of 1D NMR spectra and cross-peak amplitude processing in 2D EXSY spectra, recorded at variable temperatures. Previous studies on this propeller-shaped chiral compound show that the stereomer threshold interconversion is associated with helicity reversal and occurs through [1,2]- and [1,3]-two ring flips of one mesityl and the 2,4,6-trimethoxyphenyl rings. In the present study, the experimental rate constants of the [1,2]- and [1,3]-two ring flips, which are identical, were determined at various temperatures by combining quantitative 2D EXSY spectra processing and complete lineshape analysis (CLSA) of 1D NMR spectra. The latter were subjected to reference deconvolution and linear prediction in order to eliminate the lineshape distortions due to magnetic field inhomogeneity. The activation parameters of these ring flips were determined by an Eyring equation analysis of the temperature dependence of the rate constant. The experimentally determined activation enthalpy and entropy for the two-ring flips, and those obtained from theoretical ab initio calculations at different levels of theory and basis sets, were found to be in good agreement.
Assuntos
Derivados de Benzeno/análise , Simulação por Computador , Espectroscopia de Ressonância Magnética/métodos , Modelos Químicos , Teoria Quântica , Espectroscopia de Ressonância Magnética/normas , Conformação Molecular , Padrões de Referência , Estereoisomerismo , TemperaturaRESUMO
Novel bis(benzimidazol-2-yl)amines were synthesized using two methods and studied for antitrichinellosis activity. DFT calculations were performed in order to determine the geometry of molecules. All derivatives of 2-aminobenzimidazole exhibited higher activity in vitro against Trichinella spiralis larvae in regard to the activity of albendazole, moreover compounds 4f-i manifested antitrichinellosis effect, which surpassed five times the activity of albendazole. The in vivo screening of intestinal phase of the T. spiralis revealed 100% effectiveness of compounds 4g-i at oral dosages of 50 and 100mg/kgmw, while albendazole possesses 100% efficacy only at a dose of 100mg/kgmw.
Assuntos
Aminas/síntese química , Anti-Helmínticos/farmacologia , Benzimidazóis/síntese química , Larva/efeitos dos fármacos , Trichinella spiralis/efeitos dos fármacos , Triquinelose/tratamento farmacológico , Aminas/farmacologia , Animais , Anti-Helmínticos/síntese química , Benzimidazóis/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Enteropatias Parasitárias/tratamento farmacológico , Camundongos , Estrutura Molecular , Testes de Sensibilidade Parasitária , Trichinella spiralis/crescimento & desenvolvimento , Triquinelose/metabolismo , Triquinelose/prevenção & controleRESUMO
(1)H NMR spectroscopy was used to investigate the aggregation of the surfactant di-isobutyl-phenoxy-ethoxy-ethyl-dimethyl-benzyl ammonium methacrylate (Hyamine-M) in benzene. Adding water makes swollen reverse micelles (microemulsion droplets). The droplets also contain cadmium ions and the sodium salt of the methacrylic acid. The critical micelle concentration of Hyamine-M was determined by NMR to be 3.95 mM under the current conditions. Two-dimensional NMR NOESY spectra were used to study the conformation of the surfactant in the micelle and the spatial localization of water and counterions. We found that the surfactant molecules are folded with both phenyl fragments oriented toward the micelle exterior and the oxyethylene and NCH(3) groups in the micelle core. The water molecules and counterions are distributed around the surfactant polar groups in the micelle interior and penetrate up to both aromatic rings. The investigated system can be further utilized as a microemulsion matrix for the synthesis of cadmium-containing semiconductor nanocrystals, eventually capped with a polymer shell, or of polymer nanoparticles.
RESUMO
Using density functional theory (DFT) with the B3LYP, PBE, and PBE0 exchange-correlation functionals as well as the Moller-Plesset second-order perturbation theory (MP2) combined with a series of rather extended basis sets, 1H and 13C chemical shifts of small alkanes and chloroalkanes (with different numbers of chlorine atoms on specific positions) have been simulated and compared to experimental data. For the 1H chemical shifts, theory tends to reproduce experiment within the limits of the experimental errors. In the case of 13C chemical shift, the differences between theory and experiment increase monotonically with the number of chlorine atoms and exhibit a deviation from additivity. This behavior is related to the saturation of the experimental 13C chemical shifts with the number of chlorine atoms, whereas the evolution is mostly linear at both DFT and MP2 levels of approximation. This difference has been traced back to the relativistic spin-orbit coupling effects, which are exalted as a result of the enhancement of the s character of the C atom when increasing the number of linked Cl atoms. Thus, it was demonstrated that not only electron correlation but also relativistic effects have to be considered for estimating the 13C chemical shifts when several Cl atoms are directly attached to the C atom. Linear (theory/experiment) regressions have then been performed for the different types of C atoms, i.e., bearing one, two, and three Cl atoms, with excellent correlation coefficients. The linear correlation relationships so obtained can then serve to predict and facilitate the interpretation of the nuclear magnetic resonance spectra of more complex compounds. Furthermore, by investigating the basis set effects, the correlation between the chemical shifts calculated using the 6-311 + G(2d,p) basis set and the more extended 6-311 + G(2df,p) and aug-cc-pvtz basis sets is excellent, demonstrating that the choice of the 6-311 + G(2d,p) basis set for calculating the 1H and 13C chemical shifts is relevant.
RESUMO
Piperazine derivatives of 5(6)-substituted-(1H-benzimidazol-2-ylthio)acetic acids were synthesized by using two methods and studied for antihelminthic activity. The antiparasitic screening showed that compounds 18-24 exhibited higher activity against Trichinella spiralis in vitro in comparison to methyl 5-(propylthio)-1H-benzimidazol-2-yl-carbamate (albendazole). Most active were compounds 2-({2-[4-(4-chlorophenyl)piperazin-1-yl]-2-oxoethyl}thio)-1H-benzimidazole 21 and 2-{[2-oxo-2-(4-benzhydrylpiperazin-1-yl)ethyl]thio}-5(6)-methyl-1(H)-benzimidazole 19 as well as 2-({2-[4-(4-chlorophenyl)piperazin-1-yl]-2-oxoethyl}thio)-5(6)-methyl-1(H)-benzimidazole 23 with efficacy of 96.0%, 98.4% and 100%, respectively. The tested derivatives 15-19 and 20-23 were less active against Syphacia obvelata in vivo than albendazole and exhibited the same efficacy as piperazine, but in twice lower concentration.Compounds 2-({2-[4-(4-chlorophenyl)piperazin-1-yl]-2-oxoethyl}thio)-1H-benzimidazole 21, 1,4-bis[(5(6)-methyl-1(H)-benzimidazol-2-ylthio)acetyl]piperazine 17 and 2-({2-[4-(4-chlorophenyl)piperazin-1-yl]-2-oxoethyl}thio)-5(6)-methyl-1(H)-benzimidazole 23 had higher efficacies of 73%, 76%, and 77%, respectively.
Assuntos
Anti-Helmínticos/farmacologia , Benzimidazóis/farmacologia , Oxyuroidea/efeitos dos fármacos , Trichinella spiralis/efeitos dos fármacos , Animais , Anti-Helmínticos/efeitos adversos , Anti-Helmínticos/síntese química , Anti-Helmínticos/química , Benzimidazóis/efeitos adversos , Benzimidazóis/síntese química , Benzimidazóis/química , Fígado/efeitos dos fármacos , Espectroscopia de Ressonância MagnéticaRESUMO
The accuracy of the recently reported low-resolution NMR method (Goudappel, G. J. W.; et al. J. Colloid Interface Sci. 2001, 239, 535) for the determination of drop-size distribution in oil-in-water emulsions is evaluated by comparing the NMR results with precise data from video-enhanced optical microscopy. A series of 27 soybean-oil-in-water emulsions, differing in their mean drop size, polydispersity, oil volume fraction, and emulsifier, is studied. Soybean oil is selected as a typical component of food emulsions. The experimental error of our optical procedure for drop-size determination is estimated to be around 0.3 microm, which allows us to use the microscopy data as a reference for the mean drop-size and distribution width of the studied emulsions, with known experimental error. The main acquisition parameters in the NMR experiment are varied to find their optimal values and to check how the experimental conditions affect the NMR results. Comparison of the results obtained by the two methods shows that the low-resolution NMR method underestimates the mean drop size, d33, by approximately 20%. For most of the samples, NMR measures relatively precisely the distribution width (+/-0.1 to 0.2 dimensionless units), but for approximately 20% of the samples, larger systematic deviation was registered (underestimate by 0.3-0.4 units). No correlation is found between the emulsion properties and the relative difference between the microscopy and NMR data. Possible reasons for the observed discrepancy between NMR and optical microscopy are discussed, and some advantages and limitations of the low-resolution NMR method are considered.