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Context and objective: In Morocco, autism is a frequent disorder and no epidemiological studies have been carried out. The aim of this work is to analyze the characteristics of the onset of autism in children. Settings and Design: We conducted a cross-sectional retrospective study, spread over a period of 10 months. Methods: We included in our survey children who, according to the DSM 5 definition, had one of the autism spectrum disorders and had a well-defined diagnosis. For the statistical analysis we used Excel® software. We used the percentages for the description of the qualitative variables. Results: Of the parents of children with ASD, 49% were biological mothers. Of these, 30% women had their child when they were between 30 and 35 years of age. Males accounted for 61% of children with a sex ratio of 2.6. The first autistic traits appeared at the age of 18 months for 22% of our population. Of mothers with children with ASD, 83% had regular medical follow-up throughout their pregnancy. All autistic children in our population were vaccinated according to the National Immunization Program. According to parent reports, 70% affirmed that the first autistic features appeared after vaccination with the measles-mumps-rubella (MMR) vaccine, knowing that this statement is not based on evidence. Conclusions: The quality of care depends on the age at which the diagnosis of autism is established.
RESUMO
BACKGROUND AND OBJECTIVE: Chemical spermicides currently marketed and widely used are known to have many side effects. Thereby, and in order to look for more tolerated natural spermicidal agents, the aim of this work was to evaluate the spermicidal potential of saponin extracts from the roots of Saponaria officinalis/Caryophyllaceae, Glycyrrhizia glabra/Fabaceae, and Herniaria glabra/Caryophyllaceae by studying their in vitro effects on sperm mobility and vitality. METHODS: Methanolic saponin extracts from the plants roots were performed. Sperm suspensions were prepared by centrifugation on a PureSperm® density gradient (70 and 45%) and incubated with various concentrations of saponin extracts (50, 250, 500 and 750 µg/mL) at 37°C. The spermicidal activity was evaluated by studying the mobility and vitality of spermatozoa at different time intervals ranging from 10 to 240 minutes. RESULTS: A dose and time dependent effect on sperm mobility and vitality was observed for our extracts.Extracts from Saponaria officinalis roots induced an irreversible immobilization and a total non-viability of sperm within 10 minutes at a concentration of 750 µg/mL. A similar effect was observed within 30 minutes at 750 µg/mL for Herniaria glabra extract and within 90 minutes at 500 µg/ml for Glycyrrhizia glabra extract. CONCLUSION: The results of our study showed that the saponin extracts of our plants roots possess potent in vitro dose and time dependant spermicidal effect. These natural products could therefore represent a safer and better tolerated alternative to chemical spermicides.
RESUMO
Scolymus hispanicus or the Golden Thistle, locally known as 'Guernina' or 'Taghediwt', is one of the most appreciated wild vegetables in Morocco. This study aims to characterise the functional chemical and pharmacological variability of Scolymus hispanicus parts (roots, stems, leaves and flowers). The chemical analysis revealed higher content of α-tocopherol in the flowers (2.79 ± 0.07 mg/100 g) and lead to the identification of 3 flavonoids and 13 phenolic acids, with high content of gallic acid in leaves (187.01 ± 10.19 mg/kg); chlorogenic (936.18 ± 92.66 mg/kg) and caffeic (4400.14 ± 191.43 mg/kg) acids in flowers, roots were much more higher in sinapic acid (0.25 ± 0.03 mg/kg) compared to the other parts. Moreover, Scolymus hispanicus ethanolic extracts exhibited interesting antioxidant and antimicrobial properties, promising anti-amylase and anti-glucosidase activities and relevant diuretic effect that confirms its traditional uses.
