Disentangling drivers of litter decomposition in a multi-continent network of tree diversity experiments.

Litter decomposition is a key ecosystem function in forests and varies in response to a range of climatic, edaphic, and local stand characteristics. Disentangling the relative contribution of these factors is challenging, especially along large environmental gradients. In particular, knowledge of the effect of management options, such as tree planting density and species composition, on litter decomposition would be highly valuable in forestry. In this study, we made use of 15 tree diversity experiments spread over eight countries and three continents within the global TreeDivNet network. We evaluated the effects of overstory composition (tree identity, species/mixture composition and species richness), plantation conditions (density and age), and climate (temperature and precipitation) on mass loss (after 3 months and 1 year) of two standardized litters: high-quality green tea and low-quality rooibos tea. Across continents, we found that early-stage decomposition of the low-quality rooibos tea was influenced locally by overstory tree identity. Mass loss of rooibos litter was higher under young gymnosperm overstories compared to angiosperm overstories, but this trend reversed with age of the experiment. Tree species richness did not influence decomposition and explained almost no variation in our multi-continent dataset. Hence, in the young plantations of our study, overstory composition effects on decomposition were mainly driven by tree species identity on decomposer communities and forest microclimates. After 12 months of incubation, mass loss of the high-quality green tea litter was mainly influenced by temperature whereas the low-quality rooibos tea litter decomposition showed stronger relationships with overstory composition and stand age. Our findings highlight that decomposition dynamics are not only affected by climate but also by management options, via litter quality of the identity of planted trees but also by overstory composition and structure.

Climatic conditions, not above- and belowground resource availability and uptake capacity, mediate tree diversity effects on productivity and stability.

Tree species diversity promotes multiple ecosystem functions and services. However, little is known about how above- and belowground resource availability (light, nutrients, and water) and resource uptake capacity mediate tree species diversity effects on aboveground wood productivity and temporal stability of productivity in European forests and whether the effects differ between humid and arid regions. We used the data from six major European forest types along a latitudinal gradient to address those two questions. We found that neither leaf area index (a proxy for light uptake capacity), nor fine root biomass (a proxy for soil nutrient and water uptake capacity) was related to tree species richness. Leaf area index did, however, enhance productivity, but negatively affected stability. Productivity was further promoted by soil nutrient availability, while stability was enhanced by fine root biomass. We only found a positive effect of tree species richness on productivity in arid regions and a positive effect on stability in humid regions. This indicates a possible disconnection between productivity and stability regarding tree species richness effects. In other words, the mechanisms that drive the positive effects of tree species richness on productivity do not per se benefit stability simultaneously. Our findings therefore suggest that tree species richness effects are largely mediated by differences in climatic conditions rather than by differences in above- and belowground resource availability and uptake capacity at the regional scales.

Litter quality and the law of the most limiting: Opportunities for restoring nutrient cycles in acidified forest soils.

The adverse effects of soil acidification are extensive and may result in hampered ecosystem functioning. Admixture of tree species with nutrient rich litter has been proposed to restore acidified forest soils and improve forest vitality, productivity and resilience. However, it is common belief that litter effects are insufficiently functional for restoration of poorly buffered sandy soils. Therefore we examined the effect of leaf litter on the forest floor, soil chemistry and soil biota in temperate forest stands along a range of sandy soil types in Belgium, the Netherlands and Germany. Specifically, we address: i) Which tree litter properties contribute most to the mitigation of soil acidification effects and ii) Do rich litter species have the potential to improve the belowground nutrient status of poorly buffered, sandy soils? Our analysis using structural equation modelling shows that litter base cation concentration is the decisive trait for the dominating soil buffering mechanism in forests that are heavily influenced by atmospheric nitrogen (N) deposition. This is in contrast with studies in which leaf litter quality is summarized by C/N ratio. We suggest that the concept of rich litter is context dependent and should consider Liebig's law of the most limiting: if N is not limiting in the ecosystem, litter C/N becomes of low importance, while base cations (calcium, magnesium, potassium) become determining. We further find that on poorly buffered soils, tree species with rich litter induce fast nutrient cycling, sustain higher earthworm biomass and keep topsoil base saturation above a threshold of 30%. Hence, rich litter can trigger a regime shift to the exchange buffer domain in sandy soils. This highlights that admixing tree species with litter rich in base cations is a promising measure to remediate soil properties on acidified sandy soils that receive, or have received, high inputs of N via deposition.