RESUMO
The developing innervation of the chick eye has been studied using catecholamine histofluorescence. The innervation of the pupillary dilator by the superior cervical ganglion begins on day 13 of incubation when fluorescent axons can be seen in the ciliary zone circumscribing the dilator. On day 14 a few processes are seen to branch from this band into the dilator. The number of processes in the dilator increases on days 15 and 16. After day 16 there is a reorganization of the fibers radially accompanied by a moderate increase in the number of processes. In addition, a group of fluorescent cells can be seen in the choroid adjacent to the ciliary body. These cells are bipolar at day 9 and become multipolar by 12 days of incubation. These cells contribute to a fluorescent plexus of processes in the choroid which stops abruptly at the border of the choroid and ciliary zone. It is thought that they represent a terminal sympathetic ganglion receiving preganglionic input from the carotid nerve.
Assuntos
Embrião de Galinha/crescimento & desenvolvimento , Corioide/embriologia , Iris/embriologia , Sistema Nervoso Simpático/embriologia , Animais , Corioide/inervação , Gânglios Autônomos/embriologia , Iris/inervação , Microscopia de Fluorescência , Epitélio Pigmentado Ocular/citologiaRESUMO
The localization of 3H-opiates in the myenteric plexus of the guinea pig ileum is subject to systematic artifact when stretch preparations of the myenteric plexus are dipped into liquid Kodak NTB-3 emulsion for autoradiography. The cause of the artifact was determined to be a discontinuous distribution, or retraction, of emulsion over plexuses. The apposition of frozen freeze-dried ileal sections to dried photographic emulsion avoids this source of error.
Assuntos
Autorradiografia , Animais , Emulsões , Íleo/análise , Entorpecentes/análise , FotografaçãoRESUMO
Preferential binding of 3H-labeled morphine to satellite cells, but not to large neurons in the myenteric plexus, is demonstrated autoradiographically. Microfluorometric spectra of the plexus show nerve fibers that contain norepinephrine and impinge on satellite cells. Cells containing serotonin occur occasionally on longitudinal muscle outside the myenteric plexus.
Assuntos
Morfina/metabolismo , Plexo Mientérico/metabolismo , Receptores de Droga , Animais , Dopamina/metabolismo , Feminino , Cobaias , Plexo Mientérico/citologia , Norepinefrina/metabolismo , Serotonina/metabolismoAssuntos
Autorradiografia/métodos , Histocitoquímica/métodos , Microscopia de Fluorescência/métodos , Animais , Aminas Biogênicas/análise , Células Enterocromafins/análise , Imunofluorescência , Secções Congeladas , Técnicas Histológicas , Intestino Delgado/análise , Intestino Delgado/citologia , Masculino , Camundongos , Espectrometria de FluorescênciaAssuntos
Autorradiografia , Osso e Ossos/citologia , Química Encefálica , Encéfalo/citologia , Técnicas Histológicas , Intestinos/citologia , Dietilamida do Ácido Lisérgico/análise , Timidina/análise , Animais , Células da Medula Óssea , Osso e Ossos/análise , Cartilagem/citologia , Núcleo Celular/metabolismo , DNA/biossíntese , Estudos de Avaliação como Assunto , Liofilização , Histocitoquímica , Mucosa Intestinal/metabolismo , Intestinos/análise , Camundongos , Microtomia , Protetores contra Radiação/análise , Ratos , Timidina/metabolismo , TrítioRESUMO
Intravenous administration of [(3)H]lysergic acid diethylamide(LSD) to rats resulted in accumulation of the drug in the brain within 15 minutes. Autoradiographic methods were used to differentiate free and bound [(3)H]LSD in brain tissue. Free [(3)H]LSD was generally distributed in the pituitary and pineal glands, cerebellum, hippocampus,and choroid plexus. Bound [(3)H]LSD was localized in neurons of the cortex, caudate nucleus, midbrain, and medulla,as well as in choroid plexus epithelium.