RESUMO
Porcine proliferative enteropathy caused by Lawsonia intracellularis is an important enteric disease in swine throughout the world. Information regarding the distribution of this pathogen in Canadian swine herds would be beneficial for the creation of control protocols. Pigs from Ontario, Quebec, and Alberta were tested by using an indirect immunofluorescence assay for antibodies to L. intracellularis. Pig seroprevalence was calculated as the proportion of pigs positive from total pigs tested in the targeted population. Seroprevalence (+/- standard error [s(x)]) in market hogs in Ontario from farrow-finish (FF) farms and finishing (FIN) farms were significantly different at 77% (s(x) = 7%) and 29% (s(x) = 15%), respectively. Seroprevalence for sows and gilts in FF and farrowing and nursery (FAR + NUR) farms in Ontario were 90% (s(x) = 3%) and 93% (s(x) = 6%), respectively. Seroprevalence in breeding females in Quebec from FF and FAR farms was 82% (s(x) = 5%) and 87% (s(x) = 3%), respectively. Seroprevalence (57%, s(x) = 8%) in finishing pigs in Alberta from FF farms was significantly different from that of multisite (MS) farms and FIN farms, 6% (s(x) = 6%) and 9% (s(x) = 5%), respectively. Lawsonia intracellularis appears to be widespread in Canada and the seroprevalence on FF farms is higher than that on FIN and MS farms, possibly due to the presence of breeding females or management differences.
Assuntos
Anticorpos Antibacterianos/sangue , Infecções por Desulfovibrionaceae/veterinária , Lawsonia (Bactéria)/imunologia , Doenças dos Suínos/epidemiologia , Alberta/epidemiologia , Criação de Animais Domésticos/métodos , Animais , Infecções por Desulfovibrionaceae/epidemiologia , Feminino , Técnica Indireta de Fluorescência para Anticorpo/métodos , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Masculino , Ontário/epidemiologia , Quebeque/epidemiologia , Estudos Soroepidemiológicos , SuínosRESUMO
Milk residues and performance were evaluated in lactating cows that were fed up to 10 times the recommended dose of monensin. Following an acclimatization period of 14 d, during which cows were fed a standard lactating cow total mixed ration containing 24 ppm monensin, 18 lactating Holstein dairy cows were grouped according to the level of feed intake and then randomly assigned within each group to 1 of 3 challenge rations delivering 72, 144, and 240 ppm monensin. Outcome measurements included individual cow daily feed intakes, daily milk production, body weights, and monensin residues in composite milk samples from each cow. There were no detectable monensin residues (< 0.005 microg/mL) in any of the milk samples collected. Lactating cows receiving a dose of 72 ppm monensin exhibited up to a 20% reduction in dry matter intake, and a 5% to 15% drop in milk production from the pre-challenge period. Cows receiving doses of 144 and 240 ppm monensin exhibited rapid decreases in feed intake of up to 50% by the 2nd d and milk production losses of up to 20% and 30%, respectively, within 4 d. Lactating cows receiving up to 4865 mg monensin per day had no detectable monensin residues (< 0.005 microg/mL) in any of the milk samples collected. Results of this study confirm that food products derived from lactating dairy cattle receiving monensin at recommended levels are safe for human consumption.
Assuntos
Bovinos/fisiologia , Resíduos de Drogas/análise , Ionóforos/farmacocinética , Lactação/efeitos dos fármacos , Leite/química , Monensin/farmacocinética , Animais , Bovinos/metabolismo , Qualidade de Produtos para o Consumidor , Relação Dose-Resposta a Droga , Ingestão de Energia/efeitos dos fármacos , Feminino , Ionóforos/administração & dosagem , Lactação/fisiologia , Leite/metabolismo , Monensin/administração & dosagem , Distribuição AleatóriaRESUMO
This study was designed to assess the impact of a controlled release capsule (CRC) of monensin, administered prior to calving, on postcalving haptoglobin levels. The role of disease on haptoglobin levels was also studied. The study population consisted of 1010 cows from 25 Holstein dairy herds near Guelph, Ontario. Monensin CRC or placebo capsules were randomly assigned within each herd 3 wk prior to the expected calving date. Serum from week 1 and week 6 postcalving was submitted for quantification of haptoglobin concentrations. Haptoglobin results were analyzed for associations with treatment, health data, and individual cow factors up to 95 d in milk. Haptoglobin concentrations were higher in week 1 than week 6 (P < 0.05). In univariate analysis, several diseases were significantly associated with haptoglobin concentrations. However, occurrence of disease appeared to be a confounding factor in the data interpretation. Thus, the analysis was stratified by the presence or absence of disease. There appeared to be associations between factors other than clinical disease contributing to increased haptoglobin levels in both clinically healthy and unhealthy cattle. Haptoglobin served as a good indicator of inflammatory disease. Monensin CRC treatment was associated with increased haptoglobin concentrations in clinically unhealthy cattle, perhaps reflecting a better ability to respond to disease challenge. The lower haptoglobin concentrations in monensin CRC treated cattle that were clinically normal may be a reflection of reduced subclinical disease.
Assuntos
Doenças dos Bovinos/sangue , Haptoglobinas/efeitos dos fármacos , Ionóforos/farmacologia , Cetose/veterinária , Monensin/farmacologia , Período Pós-Parto/sangue , Animais , Bovinos , Doenças dos Bovinos/imunologia , Preparações de Ação Retardada , Feminino , Ionóforos/administração & dosagem , Cetose/sangue , Cetose/imunologia , Monensin/administração & dosagem , Paridade , Placebos , GravidezRESUMO
A field isolate of Actinobacillus pleuropneumoniae, the causative agent of porcine fibrinohemorrhagic necrotizing pleuropneumonia, was sent to the diagnostic laboratory for serotyping. The isolate presented a clear reaction, with both polyclonal antibodies against serotype 1 and monoclonal antibodies against the capsular polysaccharide of serotype 1. It also exhibited a PCR profile of Apx toxins expected for serotype 1. The isolate, however, failed to react with monoclonal antibodies against the O-antigen of serotype 1 lipopolysaccharide (LPS), suggesting a rough phenotype. The lipid A-core region of the isolate migrated faster than the corresponding region of the serotype 1 reference strain S4074 by Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis, suggesting the presence of a truncated core. Sugar analysis and mass spectrometry analysis of the O-deacylated LPS from the field isolate were consistent with the absence of O-antigen and truncation of the outer core compared to the wild-type reference strain. Experimental infection of pigs confirmed the virulence of the isolate. This is the first report of an isolate of A. pleuropneumoniae serotype 1 with a truncated outer core and a rough LPS phenotype. Veterinary diagnostic laboratories should be vigilant, since infections caused by such an isolate will not be detected by serological tests based on LPS O-antigen.
Assuntos
Infecções por Actinobacillus/veterinária , Actinobacillus pleuropneumoniae/classificação , Actinobacillus pleuropneumoniae/isolamento & purificação , Lipopolissacarídeos/metabolismo , Antígenos O , Doenças dos Suínos/microbiologia , Infecções por Actinobacillus/microbiologia , Actinobacillus pleuropneumoniae/genética , Actinobacillus pleuropneumoniae/patogenicidade , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Genótipo , Lipopolissacarídeos/química , Fenótipo , Sorotipagem , Suínos , VirulênciaRESUMO
The efficacy of tilmicosin administered in the feed to control Actinobacillus pleuropneumoniae infections in pigs was evaluated through a multisite, multitrial study. For each of 6 trials, 48 pigs (stratified by weight and sex) were randomly assigned to 6 to 8 pens. Medicated feed containing tilmicosin (200 g/t) and unmedicated feed were randomly assigned at the pen level and were provided ad libitum from day -7 to trial termination (day 14). Seeder pigs (inoculated intranasally with A. pleuropneumoniae serotype 1 and showing signs of clinical disease) were introduced to each pen on day 0. Rates of death, gross lesions, and culture of A. pleuropneumoniae at necropsy, clinical scores, average daily gain in weight, and average body temperature were compared between the medicated and unmedicated pigs. Compared with the unmedicated pigs, significantly fewer (P < 0.05) pigs given tilmicosin had lesions typical of A. pleuropneumoniae or had A. pleuropneumoniae isolated from their tissues at necropsy. Together with a significant reduction (P < 0.05) in the average percentage of pneumonic lung involvement (both visually and by weight), there were reductions in the numbers of pigs with moderate and severe pneumonic lung lesions and with A. pleuropneumoniae associated mortality. With tilmicosin treatment, the average daily weight gain, daily temperature, abdominal appearance, attitude, and respiration were also significantly better (P < 0.05). The results of this study demonstrate the in vivo effectiveness of tilmicosin (200 g/t) in controlling pleuropneumonia among swine experimentally infected with A. pleuropneumoniae.
Assuntos
Infecções por Actinobacillus/veterinária , Actinobacillus pleuropneumoniae , Macrolídeos/uso terapêutico , Doenças dos Suínos/tratamento farmacológico , Tilosina/análogos & derivados , Tilosina/uso terapêutico , Infecções por Actinobacillus/tratamento farmacológico , Administração Oral , Ração Animal , Animais , Feminino , Macrolídeos/administração & dosagem , Masculino , Quebeque , Suínos , Doenças dos Suínos/patologia , Resultado do Tratamento , Tilosina/administração & dosagemRESUMO
Data from the Food Safety Division, Alberta Agriculture, Food and Rural Development were analyzed to determine the frequency of diagnosis of porcine proliferative enteropathy (PPE) relative to the diagnosis of other porcine enteric infections between 1993 and 1997. Next to colibacillosis, PPE was the most commonly diagnosed enteric disease among those reported.