RESUMO
Aging is the biological process of declining physiologic function associated with increasing mortality rate during advancing age. Humans and higher nonhuman primates exhibit unusually longer average life spans as compared with mammals of similar body mass. Furthermore, the population of humans worldwide is growing older as a result of improvements in public health, social services, and health care systems. Comparative studies among a wide range of organisms that include nonhuman primates contribute greatly to our understanding about the basic mechanisms of aging. Based on their genetic and physiologic relatedness to humans, nonhuman primates are especially important for better understanding processes of aging unique to primates, as well as for testing intervention strategies to improve healthy aging and to treat diseases and disabilities in older people. Rhesus and cynomolgus macaques are the predominant monkeys used in studies on aging, but research with lower nonhuman primate species is increasing. One of the priority topics of research about aging in nonhuman primates involves neurologic changes associated with cognitive decline and neurodegenerative diseases. Additional areas of research include osteoporosis, reproductive decline, caloric restriction, and their mimetics, as well as immune senescence and chronic inflammation that affect vaccine efficacy and resistance to infections and cancer. The purpose of this review is to highlight the findings from nonhuman primate research that contribute to our understanding about aging and health span in humans.
Assuntos
Envelhecimento/patologia , Primatas/fisiologia , Pesquisa , Envelhecimento/genética , Animais , Humanos , Macaca fascicularis , Macaca mulatta , Modelos Animais , Primatas/genéticaRESUMO
A European goldfinch (Carduelis carduelis), a canary (Serinus canaria), and a lovebird (Agapornis roseicollis) captive-bred at three different private aviaries in Spain were submitted for necropsy with a history of weakness and ruffled feathers, weight loss associated with glossitis, and respiratory disease, respectively. Microscopically, enterocytes in the jejunum and ileum contained colonies of gram- and Stamp-positive, oval to elliptical microorganisms within parasitophorous vacuoles in the apical cytoplasm. Nested PCR using MSP primers that target microsporidian RNA genes produced amplicons of expected size for Encephalitozoon species, and analysis of forward and reverse DNA sequences confirmed the presence of Encephalitozoon hellem in all cases. The main cause of death of all three birds consisted of concurrent infections. However, intestinal encephalitozoonosis may have contributed to exacerbated catabolism. Encephalitozoonosis (or microsporidiosis) has been rarely described in passerine birds.
Assuntos
Doenças das Aves/parasitologia , Encefalitozoonose/veterinária , Agapornis/parasitologia , Animais , Doenças das Aves/diagnóstico , Doenças das Aves/patologia , Canários/parasitologia , Encephalitozoon/fisiologia , Encefalitozoonose/diagnóstico , Encefalitozoonose/parasitologia , Encefalitozoonose/patologia , Feminino , Tentilhões/parasitologia , Intestinos/parasitologia , Intestinos/patologia , Masculino , EspanhaRESUMO
Intensification of food production has the potential to drive increased disease prevalence in food plants and animals. Microsporidia are diversely distributed, opportunistic, and density-dependent parasites infecting hosts from almost all known animal taxa. They are frequent in highly managed aquatic and terrestrial hosts, many of which are vulnerable to epizootics, and all of which are crucial for the stability of the animal-human food chain. Mass rearing and changes in global climate may exacerbate disease and more efficient transmission of parasites in stressed or immune-deficient hosts. Further, human microsporidiosis appears to be adventitious and primarily associated with an increasing community of immune-deficient individuals. Taken together, strong evidence exists for an increasing prevalence of microsporidiosis in animals and humans, and for sharing of pathogens across hosts and biomes.
Assuntos
Doenças Transmissíveis Emergentes/transmissão , Cadeia Alimentar , Parasitologia de Alimentos/tendências , Microsporídios/fisiologia , Microsporidiose/transmissão , Animais , Doenças Transmissíveis Emergentes/epidemiologia , Doenças Transmissíveis Emergentes/parasitologia , Humanos , Microsporidiose/epidemiologia , Microsporidiose/parasitologiaRESUMO
INTRODUCTION: Quantification of plasma viral load (PVL) is used to monitor disease progression in SIV-infected macaques. This study was aimed at optimizing of performance characteristics of the quantitative PCR (qPCR) PVL assay. METHODS: The PVL quantification procedure was optimized by inclusion of an exogenous control hepatitis C virus armored RNA (aRNA), a plasma concentration step, extended digestion with proteinase K, and a second RNA elution step. Efficiency of viral RNA (vRNA) extraction was compared using several commercial vRNA extraction kits. Various parameters of qPCR targeting the gag region of SIVmac239, SIVsmE660, and the LTR region of SIVagmSAB were also optimized. RESULTS: Modifications of the SIV PVL qPCR procedure increased vRNA recovery, reduced inhibition and improved analytical sensitivity. The PVL values determined by this SIV PVL qPCR correlated with quantification results of SIV RNA in the same samples using the 'industry standard' method of branched-DNA (bDNA) signal amplification. CONCLUSIONS: Quantification of SIV genomic RNA in plasma of rhesus macaques using this optimized SIV PVL qPCR is equivalent to the bDNA signal amplification method, less costly and more versatile. Use of heterologous aRNA as an internal control is useful for optimizing performance characteristics of PVL qPCRs.
Assuntos
Macaca mulatta , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos , Síndrome de Imunodeficiência Adquirida dos Símios/sangue , Vírus da Imunodeficiência Símia/genética , Animais , Genoma Viral , RNA Viral/sangue , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Síndrome de Imunodeficiência Adquirida dos Símios/virologia , Carga ViralRESUMO
Disseminated encephalitozoonosis was diagnosed in 2 sibling, juvenile, cotton-top tamarins (Saguinus oedipus) and 3 sibling, neonatal, emperor tamarins (S. imperator) by use of histologic examination, histochemical analysis, electron microscopy, and polymerase chain reaction (PCR) analysis with nucleotide sequencing. All tamarins were captive born at zoos in North America and died with no premonitory signs of disease. The main pathologic findings were myocarditis (4/5), hepatitis (3/5), interstitial pneumonia (3/5), skeletal myositis (3/5), meningoencephalitis (2/5), adrenalitis (2/5), tubulointerstitial nephritis (1/5), myelitis (1/5), sympathetic ganglioneuritis (1/5), and retinitis (1/5). Central nervous system lesions were the most prominent findings in cotton-top tamarins. The inflammation was predominantly lymphocytic and suppurative in cotton-top tamarins, whereas emperor tamarins had granulomatous or lymphoplasmacytic lesions. Intralesional periodic acid-Schiff-, gram-, or acid-fast (or all 3)-positive, oval-to-elliptical shaped organisms were found in 1 cotton-top and the 3 emperor tamarins. By electron microscopy, these organisms were consistent with microsporidia of the genus Encephalitozoon. E. cuniculi genotype III was detected by PCR analysis and sequencing in paraffin-embedded brain, lung, and bone marrow specimens from the cotton-top tamarins. Although PCR results were negative for one of the emperor tamarins, their dam was seropositive for E. cuniculi by ELISA and Western blot immunodetection. These findings and recent reports of encephalitozoonosis in tamarins in Europe suggest that E. cuniculi infection may be an emerging disease in callitrichids, causing high neonatal and juvenile mortality in some colonies. The death of 2 less than 1-day-old emperor tamarins from a seropositive dam supports the likelihood of vertical transmission in some of the cases reported here.
Assuntos
Encephalitozoon cuniculi/crescimento & desenvolvimento , Encefalitozoonose/veterinária , Doenças dos Macacos/parasitologia , Saguinus , Glândulas Suprarrenais/parasitologia , Glândulas Suprarrenais/patologia , Animais , Animais Recém-Nascidos , Animais de Zoológico , Anticorpos Antiprotozoários/sangue , Western Blotting/veterinária , Encéfalo/parasitologia , Encéfalo/patologia , DNA de Protozoário/química , DNA de Protozoário/genética , Encephalitozoon cuniculi/genética , Encefalitozoonose/parasitologia , Encefalitozoonose/patologia , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Fígado/parasitologia , Fígado/patologia , Masculino , Microscopia Eletrônica de Transmissão/veterinária , Doenças dos Macacos/patologia , América do Norte/epidemiologia , Reação em Cadeia da Polimerase/veterinária , Análise de Sequência de DNARESUMO
Disseminated microsporidiosis was diagnosed in an adult female Egyptian fruit bat that died unexpectedly in a zoo. Gross findings, which were minimal, included poor body condition, bilateral renomegaly, and mottling of the liver. Histopathological lesions, which were particularly pronounced in the urogenital tract and liver, consisted primarily of inflammation associated with intracytoplasmic microsporidian spores. Polymerase chain reaction -based methods were used to establish the identity of the microsporidian as Encephalitozoon hellem. E. hellem is an emerging cause of human and avian disease, manifested mainly as opportunistic infection in immunosuppressed patients. This report describes the first documented case of E. hellem in a non-human mammalian species.
Assuntos
Quirópteros , Encephalitozoon/isolamento & purificação , Encefalitozoonose/veterinária , Microsporidiose/veterinária , Doenças Parasitárias em Animais/patologia , Animais , Encephalitozoon/fisiologia , Encefalitozoonose/patologia , Evolução Fatal , Feminino , Fígado/parasitologia , Fígado/patologia , Microsporidiose/patologia , Sistema Urogenital/parasitologia , Sistema Urogenital/patologiaRESUMO
Therapies for microsporidiosis in humans are limited, and fumagillin, which appears to be the most broadly effective antimicrosporidial drug, is considered to be moderately toxic. The purpose of this study was to apply an in vitro drug screening assay for Encephalitozoon intestinalis and Vittaforma corneae and an in vivo athymic mouse model of V. corneae infection to assess the efficacy of TNP-470 (a semisynthetic analogue of fumagillin), ovalicin, and eight ovalicin derivatives. TNP-470, ovalicin, and three of the ovalicin derivatives inhibited both E. intestinalis and V. corneae replication by more than 70% in vitro. Another three of the ovalicin derivatives inhibited one of the two microsporidian species by more than 70%. None of the treated athymic mice survived the V. corneae infection, but they did survive statistically significantly longer than the untreated controls after daily treatment with fumagillin administered at 5, 10, and 20 mg/kg of body weight subcutaneously (s.c.), TNP-470 administered at 20 mg/kg intraperitoneally (i.p.), or ovalicin administered at 5 mg/kg s.c. Of two ovalicin derivatives that were assessed in vivo, NSC 9665 given at 10 mg/kg i.p. daily also statistically significantly prolonged survival of the mice. No lesions associated with drug toxicity were observed in the kidneys or livers of uninfected mice treated with these drugs at the highest dose of 20 mg/kg daily. These results thus support continued studies to identify more effective fumagillin-related drugs for treating microsporidiosis.
Assuntos
Ácidos Graxos Insaturados/farmacologia , Microsporídios/efeitos dos fármacos , Microsporidiose/tratamento farmacológico , Sesquiterpenos/farmacologia , Animais , Cicloexanos , Avaliação Pré-Clínica de Medicamentos , Encephalitozoon/efeitos dos fármacos , Encephalitozoon/crescimento & desenvolvimento , Técnicas In Vitro , Masculino , Camundongos , Camundongos Nus , O-(Cloroacetilcarbamoil)fumagilol , Fatores de Tempo , Vittaforma/efeitos dos fármacos , Vittaforma/crescimento & desenvolvimentoRESUMO
Microsporidia are ubiquitous obligate eukaryotic intracellular parasites that are now felt to be more akin to degenerate fungi than to protozoa. Microsporidia can be highly pathogenic, causing a broad range of symptoms in humans, especially individuals who are immunocompromised. The vast majority of human cases of microsporidiosis have been reported during the past 20 years, in patients with HIV/AIDS, while only relatively rare cases have been described in immunocompetent individuals. However, microsporidia infections are being increasingly reported in patients following solid-organ transplanation, where the main symptom has been diarrhea. The authors report the first case of pulmonary microsporidial infection in an allogeneic bone marrow transplant recipient in the United States and only the second case in the world. The patient, with a history of Hodgkin disease followed by acute myelogenous leukemia received a T-cell-depleted graft, but succumbed to respiratory failure 63 days post transplantation. An open lung biopsy, taken just before death, was originally thought to show toxoplasmosis. The correct diagnosis of microsporidiosis was made postmortem by light and electron microscopy. DNA polymerase chain reaction analysis confirmed the diagnosis and furthermore revealed it to be the dog strain of the microsporidia species Encephalitozoon cuniculi. Although to date rarely diagnosed, microsporidial infection should also be considered in the differential diagnosis of, e.g., unexplained pulmonary infection in bone marrow transplant patients.
Assuntos
Transplante de Medula Óssea , Encephalitozoon cuniculi/ultraestrutura , Encefalitozoonose/patologia , Leucemia Mieloide/terapia , Pneumopatias Parasitárias/patologia , Doença Aguda , Adulto , Animais , DNA de Protozoário/análise , DNA de Protozoário/genética , Encephalitozoon cuniculi/genética , Encefalitozoonose/parasitologia , Evolução Fatal , Feminino , Humanos , Pneumopatias Parasitárias/parasitologia , Microscopia Eletrônica de Transmissão , Reação em Cadeia da Polimerase , Transplante HomólogoRESUMO
Microsporidia are single-celled, obligate intracellular parasites that were recently reclassified from protozoa to fungi. Microsporidia are considered a cause of emerging and opportunistic infections in humans, and species infecting humans also infect a wide range of animals, raising the concern for zoonotic transmission. Persistent or self-limiting diarrhea are the most common symptoms associated with microsporidiosis in immune-deficient or immune-competent individuals, respectively. Microsporidian spores appear to be relatively resistant under environmental conditions, and species of microsporidia infecting humans and animals have been identified in water sources, raising concern about water-borne transmission. Sensitive and specific immunomagnetic bead separation and PCR-based methods are being developed and applied for detecting microsporidia in infected hosts and water sources for generating more reliable prevalence data. The most effective drugs for treating microsporidiosis in humans currently include albendazole, which is effective against the Encephalitozoon species but not against Enterocytozoon bieneusi, and fumagillin, which has broader anti-microsporidia activity but is toxic in mammals, suggesting a need to identify better drugs. Strategies to capture and disinfect microsporidia in water are being developed and include filtration, coagulation, chlorination, gamma-irradiation, and ozonation.
Assuntos
Microsporídios/fisiologia , Microsporidiose/transmissão , Água/parasitologia , Zoonoses/parasitologia , Zoonoses/transmissão , Animais , Antiprotozoários/uso terapêutico , Parasitologia de Alimentos , Genoma de Protozoário , Humanos , Insetos Vetores , Microsporídios/classificação , Microsporídios/genética , Microsporídios/crescimento & desenvolvimento , Microsporidiose/tratamento farmacológico , Microsporidiose/epidemiologia , Prevalência , Abastecimento de Água , Zoonoses/epidemiologiaRESUMO
Microsporidia are obligate, intracellular protozoal parasites that can be pathogenic in immunocompromised individuals. The majority of cases of microsporidiosis have been documented in patients with HIV, and only a few case reports exist of infection in solid organ transplant patients. We report the first case of pulmonary microsporidial infection in an allogeneic bone marrow transplant recipient in the US. The patient was a recipient of a T-cell-depleted graft who succumbed to complications from respiratory failure 63 days post transplant. The diagnosis was made post mortem by electron microscopy and confirmed with PCR. Although rare, microsporidial infection should be considered in the differential diagnosis of unexplained pulmonary infection in bone marrow transplant patients.
Assuntos
Transplante de Medula Óssea/efeitos adversos , Pneumopatias Parasitárias/etiologia , Microsporidiose/etiologia , Adulto , Evolução Fatal , Feminino , Humanos , Pneumopatias Parasitárias/diagnóstico , Depleção Linfocítica , Microscopia Eletrônica , Microsporidiose/diagnóstico , Infecções Oportunistas/diagnóstico , Infecções Oportunistas/etiologia , Transplante HomólogoRESUMO
We describe the application of single-strand conformation polymorphism (SSCP) analysis to the speciation of human microsporidia after polymerase chain reaction (PCR) amplification with the panmicrosporidian primers PMP1 and PMP2. We compared the DNA extracted and amplified from different genotypes or isolates of Enterocytozoon bieneusi, Encephalitozoon cuniculi, E. hellem, and E. intestinalis plus an isolate of Vittaforma corneae. The PCR-SSCP, when performed at 20 degrees C, generated 2 bands in distinctive, reproducible patterns in polyacrylamide gels for each species of microsporidia tested, regardless of genotype or isolate. We found PCR-SSCP to be an easy and reproducible method for speciation of human microsporidia when the primer pair PMP1 and PMP2 is used.
Assuntos
DNA de Protozoário/análise , Proteínas de Membrana , Microsporídios/classificação , Microsporídios/genética , Microsporidiose/parasitologia , Proteínas do Tecido Nervoso , Animais , Primers do DNA , Eletroforese em Gel de Ágar/métodos , Fezes/parasitologia , Amplificação de Genes , Genótipo , Humanos , Proteínas Proteolipídicas Associadas a Linfócitos e Mielina , Reação em Cadeia da Polimerase , Polimorfismo Conformacional de Fita Simples , Proteolipídeos , Reprodutibilidade dos TestesRESUMO
To develop an alternative genotyping tool, the genetic diversity of Encephalitozoon hellem was examined at the polar tube protein (PTP) locus. Nucleotide sequence analysis of the PTP gene divided 24 E. hellem isolates into four genotypes, compared to two genotypes identified by analysis of the internal transcribed spacer of the rRNA gene. The four PTP genotypes differed from each other by the copy number of the 60-bp central repeat as well as by point mutations. A simple PCR test was developed to differentiate E. hellem genotypes based on the difference in the size of PTP PCR products, which should facilitate the genotyping of E. hellem in clinical samples.
Assuntos
Encephalitozoon/classificação , Encephalitozoon/genética , Encefalitozoonose/parasitologia , Proteínas de Protozoários/genética , Animais , Sequência de Bases , DNA Espaçador Ribossômico/genética , Proteínas Fúngicas , Genes de Protozoários , Genótipo , Humanos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , RNA Ribossômico/genética , Análise de Sequência de DNARESUMO
Encephalitozoon cuniculi infects a wide range of mammalian hosts. Three genotypes based on the number of GTTT repeats in the internal transcribed spacer (ITS) of the rRNA have been described, of which genotypes I and III have been identified in humans. In this study, the genetic diversity of E. cuniculi was examined at the polar tube protein (PTP) and spore wall protein I (SWP-1) loci. Nucleotide sequence analysis of the PTP gene divided 11 E. cuniculi isolates into three genotypes in congruence with the result of analysis of the ITS of the rRNA gene. The three PTP genotypes differed from one another by the copy number of the 78-bp central repeat as well as point mutations. These E. cuniculi isolates also differed from one another in the number of 15- and 36-bp repeats in the SWP-1 gene. In addition, some E. cuniculi isolates had heterogeneous copies of the SWP-1 gene with various numbers of repeats. Intragenotypic variation was also observed at the SWP-1 locus. Based on the length polymorphism and sequence diversities of the PTP and SWP-1 genes, two simple PCR tests were developed to differentiate E. cuniculi in clinical samples.
Assuntos
Encephalitozoon cuniculi/classificação , Encephalitozoon cuniculi/genética , Proteínas Fúngicas , Proteínas de Protozoários/genética , Sequências Repetitivas de Ácido Nucleico/genética , Animais , Sequência de Bases , Encefalitozoonose/parasitologia , Genes de Protozoários , Genótipo , Humanos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Coelhos , Análise de Sequência de DNAAssuntos
DNA Espaçador Ribossômico/genética , Encephalitozoon/classificação , Proteínas Fúngicas , Proteínas de Protozoários/genética , Sequências Repetitivas de Ácido Nucleico/genética , Animais , Sequência de Bases , DNA de Protozoário/análise , Cães , Encephalitozoon/genética , Genótipo , Humanos , Dados de Sequência Molecular , Coelhos , Análise de Sequência de DNAAssuntos
Encephalitozoon cuniculi/patogenicidade , Encefalitozoonose/imunologia , Doença Granulomatosa Crônica/complicações , Animais , Anticorpos Antiprotozoários/sangue , Encefalitozoonose/parasitologia , Feminino , Doença Granulomatosa Crônica/parasitologia , Imunoglobulina G/sangue , Macrófagos Peritoneais/parasitologia , CamundongosAssuntos
Aminopeptidases/antagonistas & inibidores , Encephalitozoon cuniculi/efeitos dos fármacos , Encefalitozoonose/tratamento farmacológico , Encefalitozoonose/mortalidade , Inibidores Enzimáticos/farmacologia , Inibidores Enzimáticos/uso terapêutico , Leucina/análogos & derivados , Peptídeos , Animais , Antibacterianos/farmacologia , Cicloexanos , Modelos Animais de Doenças , Encephalitozoon cuniculi/crescimento & desenvolvimento , Encefalitozoonose/parasitologia , Ácidos Graxos Insaturados/farmacologia , Feminino , Leucina/farmacologia , Leucina/uso terapêutico , Camundongos , Camundongos Nus , O-(Cloroacetilcarbamoil)fumagilol , Testes de Sensibilidade Parasitária/métodos , Sesquiterpenos/farmacologia , Sesquiterpenos/uso terapêuticoRESUMO
Microsporidia are obligate intracellular parasites, recognized as causing chronic diarrhea and systemic disease in AIDS patients, organ transplant recipients, travelers, and malnourished children. Species of microsporidia that infect humans have been detected in drinking-water sources, and methods are needed to ascertain if these microsporidia are viable and capable of causing infections. In this study, Calcofluor White M2R and Sytox Green stains were used in combination to differentiate between live (freshly harvested) and dead (boiled) Encephalitozoon cuniculi spores. Calcofluor White M2R binds to chitin in the microsporidian spore wall. Dual-stained live spores appeared as turquoise-blue ovals, while dead spores appeared as white-yellow ovals at an excitation wavelength of 395 to 415 nm used for viewing the Calcofluor stain. Sytox Green, a nuclear stain, is excluded by live spores but penetrates compromised spore membranes. Dual-stained dead spores fluoresced bright yellow-green when viewed at an excitation wavelength of 470 to 490 nm, whereas live spores failed to stain with Sytox Green. After live and dead spores were mixed at various ratios, the number of viably stained spores detected in the dual-staining procedure correlated (P = 0.0025) with the expected numbers of viable spores. Spore mixtures were also assayed for infectivity in a focus-forming assay, and a correlation (P = 0.0002) was measured between the percentage of focus-forming microsporidia and the percentage of expected infectious spores in each mixture. By analysis of variance, no statistically significant differences were measured between the percentage of viably stained microsporidia and the percentage of infectious microsporidia (P = 0.964) in each mixture. These results suggest that Calcofluor White M2R and Sytox Green stains, when used together, may facilitate studies to identify viable microsporidia.
Assuntos
Encephalitozoon cuniculi/citologia , Análise de Variância , Animais , Benzenossulfonatos , Linhagem Celular , Criança , Quitina/análise , Encephalitozoon cuniculi/patogenicidade , Encephalitozoon cuniculi/fisiologia , Corantes Fluorescentes , Humanos , Compostos Orgânicos , Esporos/citologiaRESUMO
Microsporidia are small, single-celled, obligately intracellular parasites that have caused significant agricultural losses and interference with biomedical research. Interest in the microsporidia is growing, as these organisms are recognized as agents of opportunistic infections in persons with AIDS and in organ transplant recipients. Microsporidiosis is also being recognized in children and travelers, and furthermore, concern exists about the potential of zoonotic and waterborne transmission of microsporidia to humans. This article reviews the basic biology and epidemiology of microsporidiosis in mammals.