Kinetic modeling of xylonic acid production by Gluconobacter oxydans: effects of hydrodynamic conditions.

In this study, the synthesis of xylonic acid from xylose by Gluconobacter oxydans NL71 has been investigated. According to the relationship between oxygen transfer rate and oxygen uptake rate, three different kinetic models of product formation were established and the nonlinear fitting was carried out. The results showed that G. oxydans has critical dissolved oxygen under different strain concentrations, and the relationship between respiration intensity and dissolved oxygen conformed to the Monod equation [Formula: see text]. The maximum reaction rate per unit cell mass and the theoretical maximum specific productivity of G. oxydans obtained by the kinetic model are 0.042 mol/L/h and 6.97 g/gx/h, respectively. These results will assist in determining the best balance between the airflow rate and cell concentration in the reaction and improve the production efficiency of xylonic acid.

Oxygen mass transfer enhancement by activated carbon particles in xylose fermentation media.

In this work, the effect of activated carbon particles on the production of xylonic acid from xylose by Gluconobacter oxydans in a stirred tank bioreactor was investigated. The enhancement of the oxygen transfer coefficient by activated carbon particles was experimentally evaluated under different solids volume fractions, agitation and aeration rates conditions. The experimental conditions optimized by response surface methodology (agitation speed 800 rpm, aeration rate 7 L min-1, and activated carbon 0.002%) showed a maximum oxygen transfer coefficient of 520.7 h-1, 40.4% higher than the control runs without activated carbon particles. Under the maximum oxygen transfer coefficient condition, the xylonic acid titer reached 108.2 g/L with a volumetric productivity of 13.53 g L-1 h-1 and a specific productivity of 6.52 g/gx/h. In conclusion, the addition of activated carbon particles effectively enhanced the oxygen mass transfer rate. These results demonstrate that activated carbon particles enhanced cultivation for xylonic acid production an inexpensive and attractive alternative.

Preparation and Characterization of Pendimethalin Microcapsules Based on Microfluidic Technology.

Microencapsulation of pesticides is a promising attempt to reduce environmental pollution and prevent the active ingredients from the interference of external factors. In this paper, pendimethalin microcapsules were prepared by the interfacial polymerization of 4,4-methylenediphenyl diisocyanate (MDI) and ethylenediamine (EDA) based on microfluidic technology. Effects of the microchannel structure, reaction temperature, surfactant type, and fluid flow rates were investigated and evaluated. The results showed that pendimethalin microcapsules prepared under suitable conditions had a smooth surface, good monodispersity, a high encapsulation efficiency (96.7%), and excellent thermal stability. The size and morphology control of microcapsules were realized by adjusting the flow rates of the continuous phase and the hydrophilic monomer EDA aqueous solution. The release of pendimethalin had a sustained release characteristic that was closely related to the morphology of microcapsules. Compared with the pendimethalin emulsifiable concentrate, pendimethalin microcapsules exhibited outstanding herbicidal activity in the weed control experiments. Therefore, pendimethalin microcapsules with tunable properties were successfully obtained from the microfluidic device and showed great potential in agricultural applications.

Optimization of Specific Productivity for Xylonic Acid Production by Gluconobacter oxydans Using Response Surface Methodology.

Large amounts of xylose cannot be efficiently metabolized and fermented due to strain limitations in lignocellulosic biorefinery. The conversion of xylose into high value chemicals can help to reduce the cost of commercialization. Therefore, xylonic acid with potential value in the construction industry offers a valuable alternative for xylose biorefinery. However, low productivity is the main challenge for xylonic acid fermentation. This study investigated the effect of three reaction parameters (agitation, aeration, and biomass concentration) on xylose acid production and optimized the key process parameters using response surface methodology The second order polynomial model was able to fit the experimental data by using multiple regression analysis. The maximum specific productivity was achieved with a value of 6.64 ± 0.20 g gx -1 h-1 at the optimal process parameters (agitation speed 728 rpm, aeration rate 7 L min-1, and biomass concentration 1.11 g L-1). These results may help to improve the production efficiency during xylose acid biotransformation from xylose.