RESUMO
Nasopharyngeal carcinoma (NPC) is a malignancy with a high metastatic ability. Recent studies have implicated the role of chromodomain helicase/ATPase DNA binding protein 1-like (CHD1L) gene as a novel oncogene; however, the functional role of CHD1L in NPC remains unknown. The aim of this study was to evaluate the clinical significance of CHD1L positivity in NPC. CHD1L protein expression was examined by performing western blot analysis of 30 fresh NPC tissues and conducting immunohistochemistry tests of 133 NPC samples between December 1, 2005 and December 1, 2009. The correlations of CHD1L expression status with clinicopathological features and prognosis were investigated. Immunohistochemical analysis showed that 88 of 133 (66.2%) paraffin-embedded NPC biopsies exhibited positive expression of CHD1L, but all non-cancerous nasopharyngeal specimens were negative for CHD1L expression. In addition, positive CHD1L expression was strongly associated with an advanced clinical stage (P=0.016), recurrence (P=0.002) and the metastasis status (P=0.031) of NPC. Kaplan-Meier survival analysis demonstrated that patients with CHD1L-positive NPC had significantly shorter overall survival (P<0.001). Furthermore, the multivariate analysis indicated that CHD1L protein expression was an independent prognostic factor for overall survival (hazard ratio, 7.916; 95% confidence interval, 2.067-16.034; P=0.003) in patients with NPC. These results indicate that CHD1L is a prognostic marker for NPC.
RESUMO
OBJECTIVE: To optimize the water processing (shui-fei) condition of Realgar. METHODS: The processing conditions were optimized by L9 (3)4 orthogonal design with three factors as water dose, process times and dryness temperature. The content of dissolved arsenic As(III) in Realgar was tested by High Performance Liquid Chromatography-hydride generation on-line coupled with atomic fluorescence spectrometry (HPLC-HG-AFS). RESULTS: The optimized conditions were 15 times quantity of water, 8 times for processing and dryness temperature of 40 degrees C. CONCLUSION: The method is accurate and effective,which can be used to evaluate the quality of processed products of Realgar.