Three-Dimensional Sulfated Bacterial Cellulose/Gelatin Composite Scaffolds for Culturing Hepatocytes.

The liver is the hub of human metabolism and involves many diseases. To better work on the mechanism and treatment of liver diseases, it is of particular interest to design 3-dimensional scaffolds suitable for culturing hepatocytes in vitro to simulate their metabolic and regenerative abilities. In this study, sulfated bacterial cellulose (SBC) was prepared as the building block of cell scaffolds, motivated by the anionic nature and 3-dimensional structure of hepatic extracellular matrix, and its reaction condition for sulfate esterification was optimized by changing the reaction time. The analysis and study of the microscopic morphology, structure, and cytocompatibility of SBCs showed that they possess good biocompatibility and meet the requirements for tissue engineering. Next, SBC was mixed with gelatin for composite scaffolds (SBC/Gel) for culturing hepatocytes by homogenization and freeze-drying methods, whose physical properties such as pore size, porosity, and compression properties were compared with gelatin (Gel) scaffolds as the control group, and the cytological activity and hemocompatibility of the composite scaffolds were investigated. The results showed that the SBC/Gel composite has better porosity and compression properties, as well as good cytocompatibility and hemocompatibility, and could be applied to 3-dimensional culture of hepatocytes for drug screening or liver tissue engineering.

Bacterial cellulose-based biomaterials: From fabrication to application.

Driven by its excellent physical and chemical properties, BC (bacterial cellulose) has achieved significant progress in the last decade, rendering with many novel applications. Due to its resemblance to the structure of extracellular matrix, BC-based biomaterials have been widely explored for biomedical applications such as tissue engineering and drug delivery. The recent advances in nanotechnology endow further modifications on BC and generate BC-based composites for different applications. This article presents a review on the research advancement on BC-based biomaterials from fabrication methods to biomedical applications, including wound dressing, artificial skin, vascular tissue engineering, bone tissue regeneration, drug delivery, and other applications. The preparation of these materials and their potential applications are reviewed and summarized. Important factors for the applications of BC in biomedical applications including degradation and pore structure characteristic are discussed in detail. Finally, the challenges in future development and potential advances of these materials are also discussed.