Reference intervals for serum amyloid A, haptoglobin, ceruloplasmin, and fibrinogen in apparently healthy neonatal lambs.

BACKGROUND: Acute-phase proteins (APPs) are considered biomarkers for diagnoses, prognoses, and treatment and health status monitoring. Reference intervals (RIs) are an integral part of diagnostic laboratory testing and clinical decision-making. The RIs for neonatal lambs are lacking. OBJECTIVE: This study aimed to determine the RIs of serum amyloid A (SAA), haptoglobin (Hp), ceruloplasmin (Cp), and fibrinogen (Fb) concentrations in neonatal cross-bred Kivircik lambs. METHODS: The study consisted of 42 neonatal cross-bred Kivircik lambs. Physical examinations were performed daily, and blood samples were collected at 0 (precolostrum), 1, 3, 7, 14, 21, and 28 days of age. The RIs of SAA, Hp, Cp, and Fb concentrations were calculated according to the recommendation of the American Society for Veterinary Clinical Pathology. Results were also evaluated for age- and sex-related effects. RESULTS: The RI for serum Hp was 0-0.4 g/L, and no apparent effect of age or sex was noted. Because of significant age- and sex-related differences, the SAA (0.34-55.12 µg/mL), Cp (2.4-26.07 mg/mL), and Fb (218.86-527.82 mg/mL) results were given as minimum and maximum values. Serum SAA on the 1st, 3rd, and 7th days of life, and Cp and Fb concentrations on the 14th and 21st days of life, were significantly higher than those on other study days. CONCLUSIONS: This study is the first to establish RIs for neonatal lambs, and the values obtained could be useful for making clinical decisions regarding cross-bred Kivircik lambs during this critical period.

Molecular Identification and Clinicopathological Findings of Hepatozoon sp. Infection in a Cat: First Report from Turkey.

Hepatozoon is a genus of protozoa belonging to the phylum Apicomplexa. Ticks are the vectors for the members of this genus. The protozoans infect a wide variety of mammals, birds, reptiles, and amphibians. The domestic and wild felids are also susceptible to Hepatozoon infection. A five-year-old female cat was presented to the Adnan Menderes University, Faculty of Veterinary Medicine Animal Hospital, with a 1-month history of inappetence and weight loss. In a physical examination, weakness, depression, anorexia, lymphadenopathy, icteric mucous membranes, abdominal distension, and fever were detected. Laboratory analysis revealed anemia, neutrophilic leukocytosis, and increased serum total bilirubin and indirect bilirubin concentrations. Hepatozoonosis was diagnosed by the observation of Hepatozoon spp. gamonts within neutrophils in Giemsa-stained blood smears and confirmed by polymerase chain reaction (PCR). This is the first report about the molecular identification of hepatozoonosis in a cat from Turkey.

Haptoglobin and serum amyloid-A concentrations and their relationship with oocyst count in neonatal lambs experimentally infected with Cryptosporidium parvum.

This study aimed to evaluate the acute phase response (APR) through haptoglobin (Hp) and serum amyloid A (SAA) concentrations in serum and to examine the correlation between these acute phase proteins (APPs) and oocyst shedding using experimental Cryptosporidium parvum (C. parvum) infection model in neonatal lambs. Twenty lambs were divided into two equal groups: group CON remained uninfected as negative control and lambs of the group EXP were inoculated orally with 1×106C. parvum oocysts. Blood and faecal samples were obtained from both groups before colostrum intake and prior to inoculation (day-1), and at 2, 6, 13, and 20days post-inoculation (dpi). The serum concentrations of SAA increased following the experimental infection of lambs with C. parvum, the difference being statistically significant from pre-inoculation levels at 2 dpi, while significant increases in serum concentration of Hp were observed at 2 and 6 dpi. At the same occasions, serum concentrations of both APPs were significantly higher in the C. parvum-infected lambs compared to the healthy control lambs. A moderate positive correlation (rho=0.67; p< 0.001) was observed between serum Hp concentration and oocyst count (OPG),whereas the serum SAA concentration didn't significantly correlate with OPG (rho=0.18; p>0.05). In conclusion, the results of the study shed some light on APR due to C. parvum infection in neonatal lambs.