RESUMO
We studied the effect of early accumulation of N-3-oxo-dodecanoyl-homoserine lactone on the suppression of Pseudomonas aeruginosa reproduction, biofilm formation, and elastase activity. N-3-oxo-dodecanoyl-homoserine lactone in various concentrations was added to the P. aeruginosa culture, and changes in the concentration of bacteria and the formation of biofilms were studied in dynamics. N-3-oxo-dodecanoyl-homoserine lactone in a concentration of 25 µM, decelerated proliferation of bacterial cells during the first 6 h of culturing (p<0.05) and stimulated biofilm formation after 18 h of culturing. Elastase activity of P. aeruginosa increased significantly after addition of N-3-oxo-dodecanoyl-homoserine lactone in a concentration of 0.75 µM.
Assuntos
4-Butirolactona/análogos & derivados , Proteínas de Bactérias/metabolismo , Biofilmes/efeitos dos fármacos , Homosserina/análogos & derivados , Elastase Pancreática/metabolismo , Pseudomonas aeruginosa/efeitos dos fármacos , 4-Butirolactona/biossíntese , 4-Butirolactona/farmacologia , Carga Bacteriana , Biofilmes/crescimento & desenvolvimento , Meios de Cultura/química , Meios de Cultura/farmacologia , Relação Dose-Resposta a Droga , Homosserina/biossíntese , Homosserina/farmacologia , Pseudomonas aeruginosa/enzimologia , Pseudomonas aeruginosa/crescimento & desenvolvimento , Percepção de Quorum/fisiologiaRESUMO
The results of development of a method for detection and genotyping of the bacteria Pasteurella multocida capsular five groups and Mannheimia haemolytica Al based on the multiplex polymerase chain reaction (PCR) with electrophoretic detection are submitted. Diagnostic sensitivity of the developed method was 103 CFU/ml in the study of the pure cultures and 105 CFU/g in the study of biological material. A study of 260 samples of biological material from infected animals revealed Pasteurella multocida in 50.0%, and Mannheimia haemolytica in 11.2% of the investigated samples. Circulation among the tested livestock of capsular groups B and E of Pasteurella multocida was not revealed. The majority of the tested samples contained group A, in some cases, group D, and, in one case, group F. On the basis of the phylogenetic analysis circulation of two different genetic types of Pasteurella multocida of the capsular group A was revealed.
Assuntos
Técnicas de Tipagem Bacteriana , Técnicas de Genotipagem , Mannheimia haemolytica/genética , Pasteurella multocida/genética , Filogenia , Reação em Cadeia da Polimerase , Animais , Bovinos , Mannheimia haemolytica/classificação , Mannheimia haemolytica/isolamento & purificação , Pasteurella multocida/classificação , Pasteurella multocida/isolamento & purificaçãoRESUMO
Bovine viral diarrhea (BVD) is one of the greatest challenges for breeding and commercial livestock. It is characterized by lesions of the respiratory and gastrointestinal tract, abortion, infertility, immune deficiency, and persistence of the pathogen. In this work, a set of measures for the rehabilitation and prevention of BVD in cattle is described. It includes the data of the literature, guidance documents for the diagnosis and control of BVD adopted by OIE, EU countries, USA, as well as the results of this research.
Assuntos
Anticorpos Antivirais/sangue , Doença das Mucosas por Vírus da Diarreia Viral Bovina/prevenção & controle , Doenças dos Bovinos/prevenção & controle , Vírus da Diarreia Viral Bovina/imunologia , Monitoramento Epidemiológico/veterinária , Animais , Anticorpos Antivirais/imunologia , Doença das Mucosas por Vírus da Diarreia Viral Bovina/epidemiologia , Doença das Mucosas por Vírus da Diarreia Viral Bovina/imunologia , Doença das Mucosas por Vírus da Diarreia Viral Bovina/virologia , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/virologia , Vírus da Diarreia Viral Bovina/isolamento & purificação , Feminino , Genótipo , Tolerância Imunológica , Masculino , Tipagem Molecular , Gravidez , Federação Russa/epidemiologia , Vacinas Virais/administração & dosagemRESUMO
The protective properties of artificial mycobacterial particles versus BCG vaccine were studied in laboratory animals with experimental tuberculosis. The findings of the decreased rate of a tuberculous process and on the increased mean life span in animals inoculated with M. bovis suggest that immunization of guinea-pigs with mycobacterial particles promotes the enhanced development of antituberculous immunity in the animals. The paper proposes a promising method for designing artificial immunogens, the high-polymer antigenic structures that imitates mycobacterial particles.