Soil to earthworm bioaccumulation of polyhalogenated carbazoles and related compounds: Lab and field tests.

Polyhalogenated carbazoles (PHCZs) are an emerging group of organic contaminants that have attracted attention because of their ubiquity, resistance to biodegradation, and toxicities. However, studies on the bioaccumulation of PHCZs in terrestrial organisms are limited. In the present study, bioaccumulation of 11 PHCZs and two related compounds, carbazole (CZ) and benzocarbazole (BZCZ), from soil to earthworms was investigated by paired soil-earthworm samples from Hangzhou, China and a laboratory bioaccumulation test. The sum of the concentrations of the 11 polyhalogenated carbazoles (Σ11PHCZs), CZ and BZCZ in soils from Hangzhou were 1.78-67.27 ng/g dry weight, 1.11-57.90 ng/g dry weight, and 22.87-171.98 ng/g dry weight, respectively, while those in the earthworms were 179.49-892.90 ng/g lipid weight, 42.90-2140.42 ng/g lipid weight, and not detectable-2514.76 ng/g lipid weight, respectively. The average in situ biota-to-soil accumulation factors (BSAFs) ranged from 0.38 to 13.23, comparable to those in some reports for polychlorinated biphenyls and polybrominated diphenlethers. Site-independence of BSAFs and no correlation between log Cworm and log Csoil together support the hypothesis that distribution of PHCZs between soil and worms in Hangzhou didn't reach equilibrium. In the laboratory test, the accumulation trends of CZ, BZCZ, 3-bromocarbazole, 3,6-dichlorocarbazole, and 2,7-dibromocarbazole well fit to the first-order kinetics, with r2 ranging from 0.796 to 0.997. The BSAFs under two exposure concentration groups at steady-state conditions were 38.8-56.0 and 2.1-4.4, respectively, suggesting the capacity of bioaccumulation for these compounds. Enhancement of concentrations and resident time of the chemicals in soil would reduce the BSAF values, which may be related to the change of uptake process of the compound or redistribution of compound between soil and earthworm. A comparison of the theoretical steady-state concentrations with the nonlinear regression-based concentrations indicates that increasing the exposure time beyond 28 days is beneficial for studying the bioaccumulation of PHCZs.

Spatial distribution and implications to sources of halogenated flame retardants in riverine sediments of Taizhou, an intense e-waste recycling area in eastern China.

Concentrations and spatial distribution pattern of organohalogen flame retardants were investigated in the riverine surface sediments from Taizhou, an intensive e-waste recycling region in China. The analytes were syn- and anti- Dechlorane Plus (DP), Dechloranes 602, 603, and 604, a DP monoadduct, two dechlorinated DPs and 8 congeners of polybrominated diphenyl ethers (PBDEs). The concentrations of Σ8PBDEs, ΣDP, ΣDec600s, and ΣDP-degradates ranged from <100 to 172,000, 100 to 55,000, not detectable (nd) to 1600, and nd to 2800 pg/g dry weight, respectively. BDE-209 and DP, both have been manufactured in China, had similar spatial distribution patterns in the study area, featured by distinctly recognizable hotspots some of which are in proximity to known e-waste dumping or metal recycling facilities. Such patterns were largely shared by Dec602 and dechlorinated DP, although their concentration levels were much lower. These major flame retardants significantly correlate with each other, and cluster together in the loading plot of principle component analysis. In contrast, most non-deca PBDE congeners do not correlate with DPs. Dec604 stood out having distinctly different spatial distribution pattern, which could be linked to historical use of mirex. Organic matter content of the sediment was not the dominant factor in determining the spatial pattern of pollution by halogenated flame retardants in the rivers of this study.