RESUMO
Bombyx mori cypovirus (BmCPV) is one of the most important pathogens causing severe disease to silkworm. Emerging evidence indicates that long noncoding RNAs (lncRNAs) play importantly regulatory roles in virus infection and host immune response. To better understand the interaction between silkworm, Bombyx mori and BmCPV, we performed a comparative transcriptome analysis on lncRNAs and mRNAs between the virus-infected and noninfected silkworm larvae midgut at two time points postinoculation. A total of 16,753 genes and 1845 candidate lncRNAs were identified, among which 356 messenger RNA (mRNAs) and 41 lncRNAs were differentially expressed (DE). Target gene prediction revealed that most of DEmRNAs (123) were coexpressed with 28 DElncRNAs, suggesting that the expression of mRNA is mainly affected through trans- regulation by BmCPV-induced lncRNAs, and a regulatory network of DElncRNAs and DEmRNAs was then constructed. According to the network, many genes involved in apoptosis, autophagy, and antiviral response, such as ATG3, PDCD6, IBP2, and MFB1, could be targeted by different DElncRNAs, implying the essential roles of these genes and lncRNAs in BmCPV infection. In all, our studies revealed for the first time the alteration of lncRNA expression in BmCPV-infected larvae and its potential influence on BmCPV replication, providing a new perspective for host-cypovirus interaction studies.
Assuntos
Bombyx , RNA Longo não Codificante , Viroses , Animais , Bombyx/genética , Bombyx/imunologia , Bombyx/metabolismo , Bombyx/virologia , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Redes Reguladoras de Genes , Genes de Insetos , Interações entre Hospedeiro e Microrganismos , Imunidade , Larva/genética , Larva/imunologia , Larva/metabolismo , RNA Longo não Codificante/isolamento & purificação , RNA Longo não Codificante/metabolismo , RNA Mensageiro/metabolismo , Reoviridae , Viroses/imunologia , Viroses/metabolismoRESUMO
Based on the requirement of scientifically managing landscape ecological data and dynamically monitoring regional landscape pattern, and with the Zhifanggou region in North Shaanxi Province as an example, this paper developed a regional landscape pattern monitoring information system (RLPMIS) by the combination of Delphi and MapX. The planning route and establishing method of the system were simply explained, and its basic structure and function were explicated. This system was mainly divided into four parts, i.e., data management module, query management module, landscape index analysis module, and landscape forecasting module. In this system, the graphics and the attributes of the landscape data were integrated entirely and managed effectively, and thus, the spatial information could be displayed, located and inquired in the RLPMIS. The landscape pattern could be comprehensively analyzed based on the various landscape indexes of landscape elements or whole landscape being calculated, and the landscape developing trend could be forecasted and simulated. At the same time, the statistical graph and table, which visually reflected the landscape pattern, developed and changed disciplinarian in different periods, could be dynamically generated.