RESUMO
Spectral library search can enable more sensitive peptide identification in tandem mass spectrometry experiments. However, its drawbacks are the limited availability of high-quality libraries and the added difficulty of creating decoy spectra for result validation. We describe MS Ana, a new spectral library search engine that enables high sensitivity peptide identification using either curated or predicted spectral libraries as well as robust false discovery control through its own decoy library generation algorithm. MS Ana identifies on average 36% more spectrum matches and 4% more proteins than database search in a benchmark test on single-shot human cell-line data. Further, we demonstrate the quality of the result validation with tests on synthetic peptide pools and show the importance of library selection through a comparison of library search performance with different configurations of publicly available human spectral libraries.
Assuntos
Biblioteca de Peptídeos , Software , Humanos , Peptídeos/análise , Proteínas/química , Algoritmos , Bases de Dados de ProteínasRESUMO
The investigation of microbial proteins by mass spectrometry (metaproteomics) is a key technology for simultaneously assessing the taxonomic composition and the functionality of microbial communities in medical, environmental, and biotechnological applications. We present an improved metaproteomics workflow using an updated sample preparation and a new version of the MetaProteomeAnalyzer software for data analysis. High resolution by multidimensional separation (GeLC, MudPIT) was sacrificed to aim at fast analysis of a broad range of different samples in less than 24 h. The improved workflow generated at least two times as many protein identifications than our previous workflow, and a drastic increase of taxonomic and functional annotations. Improvements of all aspects of the workflow, particularly the speed, are first steps toward potential routine clinical diagnostics (i.e., fecal samples) and analysis of technical and environmental samples. The MetaProteomeAnalyzer is provided to the scientific community as a central remote server solution at www.mpa.ovgu.de.
RESUMO
Standard proteomics workflows use tandem mass spectrometry followed by sequence database search to analyze complex biological samples. The identification of proteins carrying post-translational modifications, for example, phosphorylation, is typically addressed by allowing variable modifications in the searched sequences. Accounting for these variations exponentially increases the combinatorial space in the database, which leads to increased processing times and more false positive identifications. The here-presented tool PhoStar identifies spectra that originate from phosphorylated peptides before database search using a supervised machine learning approach. The model for the prediction of phosphorylation was trained and validated with an accuracy of 97.6% on a large set of high-confidence spectra collected from publicly available experimental data. Its power was further validated by predicting phosphorylation in the complete NIST human and mouse high collision-dissociation spectral libraries, achieving an accuracy of 98.2 and 97.9%, respectively. We demonstrate the application of PhoStar by using it for spectra filtering before database search. In database search of HeLa samples the peptide search space was reduced by 27-66% while finding at least 97% of total peptide identifications (at 1% FDR) compared with a standard workflow.
Assuntos
Fosfopeptídeos/análise , Espectrometria de Massas em Tandem/métodos , Animais , Bases de Dados de Proteínas , Células HeLa , Humanos , Camundongos , Fosforilação , Processamento de Proteína Pós-Traducional , Aprendizado de Máquina SupervisionadoRESUMO
Biochemical reactions are subject to stochastic fluctuations that can give rise to cell-to-cell variability. Yet, how this variability affects viral infections, which themselves involve noisy reactions, remains largely elusive. Here we present single-cell experiments and stochastic simulations that reveal a large heterogeneity between influenza A virus (IAV)-infected cells. In particular, experimental data show that progeny virus titres range from 1 to 970 plaque-forming units and intracellular viral RNA (vRNA) levels span three orders of magnitude. Moreover, the segmentation of IAV genomes seems to increase the susceptibility of their replication to noise, since the level of different genome segments can vary substantially within a cell. In addition, simulations suggest that the abortion of virus entry and random degradation of vRNAs can result in a large fraction of non-productive cells after single-hit infection. These results challenge current beliefs that cell population measurements and deterministic simulations are an accurate representation of viral infections.