RESUMO
Phenylbutazone (PBZ) is permitted to be used for the treatment of musculoskeletal pain and inflammation in race horses but it is not approved for use in horses destined for human consumption. In a recent study initiated in our laboratory to study the disposition of PBZ and its oxyphenbutazone (OXPBZ) metabolite in equine tissues, we compared the effect of an additional enzymatic hydrolysis step with ß-glucuronidase on the results of the analysis for PBZ without enzymatic hydrolysis. Incurred tissue samples obtained from a female horse dosed with PBZ at 8.8 mg/kg for 3 days and sacrificed 6 days following the last administration were used for this study. Liver, kidney, and muscle tissues were collected, extracted, cleaned up on a silica-based solid-phase extraction (SPE) preceded by a weak-anion exchange SPE and analyzed with our in-house validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for PBZ and OXPBZ. Addition of the hydrolysis step resulted in a significant increase in recovery of both PBZ and OXPBZ residues. © 2016 Her Majesty the Queen in Right of Canada. Drug Testing and Analysis © 2016 John Wiley & Sons, Ltd.
Assuntos
Anti-Inflamatórios não Esteroides/análise , Resíduos de Drogas/análise , Cavalos/metabolismo , Oxifenilbutazona/análise , Fenilbutazona/análise , Espectrometria de Massas em Tandem/métodos , Animais , Anti-Inflamatórios não Esteroides/metabolismo , Anti-Inflamatórios não Esteroides/farmacocinética , Cromatografia Líquida/métodos , Resíduos de Drogas/metabolismo , Resíduos de Drogas/farmacocinética , Feminino , Contaminação de Alimentos/análise , Análise de Perigos e Pontos Críticos de Controle/métodos , Humanos , Hidrólise , Rim/química , Rim/metabolismo , Fígado/química , Fígado/metabolismo , Músculos/química , Músculos/metabolismo , Oxifenilbutazona/metabolismo , Oxifenilbutazona/farmacocinética , Fenilbutazona/metabolismo , Fenilbutazona/farmacocinética , Extração em Fase Sólida/métodos , Distribuição TecidualRESUMO
The local delivery of antimicrobials is a valuable therapeutic tool with a low morbidity, is practical to use, and is well tolerated by horses. Clinically, its use has allowed equine practitioners to achieve better results when treating musculoskeletal infections, and it represents an extremely useful tool in the practitioner's armamentarium against these types of infections. The technique is indicated to combat orthopedic infections involving bones, joints, physes, tendon sheaths, and foot tissues. Optimal treatment must include other approaches, such as systemic antimicrobial therapy and surgical debridement and lavage, and monitoring of the clinical progression of the patient can help to determine the ideal protocol for each patient.