Stimulation of human immunodeficiency virus expression in permanent monocytic cells by Sarcocystis gigantea extract.

As recently reported, a strong stimulation of noninfected CD4+ H9- cells by Sarcocystis gigantea (syn. S. ovifelis) extract (SGE) was observed using the lymphocyte proliferation assay. After SGE prestimulation, human immunodeficiency virus (HIV)-infected H9+ cells showed an exacerbation of viral replication. In the present study we investigated the reactivity of HIV-infected human monocytes using SGE. The highly sensitive p24 core-profile enzyme-linked immunosorbent assay (ELISA) was used to examine directly the amount of HIV produced. Experiments were performed using U937 permanent monocytic cells. Permanent incubation as well as preincubation with SGE before virus infection stimulated HIV expression in all the cells. In U937 cells the viral release per cell was 64 times higher on permanent stimulation with 320 microg SGE relative to controls and 9 times higher following prestimulation.

[Morphological studies of Babesia divergens during continuous transfer in Meriones unguiculatus]. / Morphologische Untersuchungen an Babesia divergens beim kontinuierlichen Transfer in Meriones unguiculatus.

A strain of Babesia divergens was propagated by inoculation of 1.5 x 10(7) parasitized erythrocytes in 30 passages under same conditions on altogether 150 jirds. The developmental stages of Babesia divergens revealed pleomorphism in the erythrocytes of the jird. Single parasites were observed as being pyriform, globular, budding, ring-like or amoebid. The shape of dividing parasites varied between the spherical and the pyriform. The stages in the erythrocytes were localized predominantly intermedia. The reduction of the number of dividing forms and the increase of single forms occurred with increasing numbers of passages. Length and breadth of double pyriforms increased by approximately 20%.

A simple method for the purification of Eimeria tenella sporozoites.

A rapid, simple and cheap method for the purification of Eimeria tenella sporozoites has been developed using commercially available filter paper (595 filter paper circles, Schleicher & Schuell, 3354 Dassel, Germany, order 311610). Yield and purity are of the same value as by purification with a leukopac column (Bontemps & Yvore 1974). The described method can be used for the purification of Eimeria tenella sporozoites under sterile conditions for subsequent in vitro cultivation.

[Immunoprophylactic possibilities for the control of fowl coccidiosis]. / Immunprophylaktische Möglichkeiten bei der Bekämpfung der Geflügelkokzidiose.

Coccidiosis is a poultry disease causing considerable economic damage in poultry farming. At present coccidiosis is controlled by chemoprophylaxes. The problem of drug resistance has led to a growing interest in the development of vaccines for the control of these parasites. A review of new methods and developments in the immunological control of coccidiosis in poultry is given and discussed.