RESUMO
Immunotherapy is restricted by a complex tumor immunosuppressive microenvironment (TIM) and low drug delivery efficiency. Herein, a multifunctional adjuvant micelle nanosystem (PPD/MPC) integrated with broken barriers and re-education of three classes of immune-tolerant cells is constructed for cancer immunotherapy. The nanosystem significantly conquers the penetration barrier via the weakly acidic tumor microenvironment-responsive size reduction and charge reversal strategy. The detached core micelle MPC could effectively be internalized by tumor-associated macrophages (TAMs), tumor-infiltrating dendritic cells (TIDCs), and myeloid-derived suppressor cells (MDSCs) via mannose-mediated targeting endocytosis and electrostatic adsorption pathways, promoting the re-education of immunosuppressive cells for allowing them to reverse from pro-tumor to antitumor phenotypes by activating TLR4/9 pathways. This process in turn leads to the remodeling of TIM. In vitro and in vivo studies collectively indicate that the adjuvant micelle-based nanosystem not only relieves the intricate immune tolerance and remodels TIM via reprogramming the three types of immunosuppressive cells and regulating the secretion of relevant cytokines/immunity factors but also strengthens immune response and evokes immune memory, consequently suppressing the tumor growth and metastasis.
Assuntos
Micelas , Neoplasias , Humanos , Imunoterapia , Imunossupressores/farmacologia , Adjuvantes Imunológicos/farmacologia , Adjuvantes Imunológicos/uso terapêutico , Neoplasias/terapia , Microambiente Tumoral , Linhagem Celular TumoralRESUMO
Chemodynamic therapy (CDT) is seriously limited by the inadequacy of exogenous catalytic ions and endogenous H2O2 in tumors. Herein, a multifunction nano-bomb integrated with calcium peroxide (CaO2) and ß-lapachone as donors of H2O2 and GSH-sensitive Fe-based coordination polymer as provider of catalytic ions was constructed for dual cascade-amplified tumor CDT. This hyaluronic acid (HA)-modified nano-bomb could be specially endocytosed by breast cancer cells through a targeting pathway, degraded and released cargoes in response to the GSH-rich cytoplasm. Furthermore, the released CaO2 and ß-lapachone could significantly self-generated sufficient H2O2, which could dual-cascade amplify CDT and induce severe oxidative to tumors via cooperating with the delivered iron ions from nano-bombs. Moreover, the unloaded iron and calcium ions could further accelerate tumor damage by overloading Ca2+ and ferroptosis, as accompanied by good magnetic resonance imaging (MRI). In vitro and in vivo studies collectively reveal that this nano-bomb not only self-initiates double cascade-amplified CDT via self-generation of H2O2, but also efficiently activates ferroptosis and initiates Ca2+ overloading, consequently significantly tumor growth suppression. This study offers a novel tumor-initiated nano-bomb for dual cascade-amplified CDT and bioimaging with activated ferroptosis and self-supplying H2O2.
Assuntos
Ferroptose , Neoplasias , Humanos , Peróxido de Hidrogênio , Ferro , Linhagem Celular TumoralRESUMO
Electrochemical cells that incorporate aluminum (Al) as the active material have become increasingly popular due to the advantages of high energy density, cost-effectiveness, and superior safety features. Despite the progress made by research groups in developing rechargeable Al//MxOy (M = Mn, V, etc.) cells using an aqueous Al trifluoromethanesulfonate-based electrolyte, the reactions occurring at the Al anode are still not fully understood. In this study, we explore the artificial solid electrolyte interphase (ASEI) on the Al anode by soaking it in AlCl3/urea ionic liquid. Surprisingly, our findings reveal that the ASEI actually promotes the corrosion of Al by providing chloride anions rather than facilitating the transport of Al3+ ions during charge/discharge cycles. Importantly, the ASEI significantly enhances the cycling stability and activity of Al cells. The primary reactions occurring at the Al anode during the charge/discharge cycle were determined to be irreversible oxidation and gas evolution. Furthermore, we demonstrate the successful realization of urea-treated Al (UTAl)//AlxMnO2 cells (discharge operating voltage of â¼1.45 V and specific capacity of 280 mAh/g), providing a platform to investigate the underlying mechanisms of these cells further. Overall, our work highlights the importance of ASEI in controlling the corrosion of Al in aqueous electrolytes, emphasizing the need for the further development of electrolytic materials that facilitate the transport of Al3+ ions in rechargeable Al batteries.
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Tumor-dependent glucose and glutamine metabolisms are essential for maintaining survival, while the accordingly metabolic suppressive therapy is limited by the compensatory metabolism and inefficient delivery efficiency. Herein, a functional metal-organic framework (MOF)-based nanosystem composed of the weakly acidic tumor microenvironment-activated detachable shell and reactive oxygen species (ROS)-responsive disassembled MOF nanoreactor core is designed to co-load glycolysis and glutamine metabolism inhibitors glucose oxidase (GOD) and bis-2-(5-phenylacetmido-1,2,4-thiadiazol-2-yl) ethyl sulfide (BPTES) for tumor dual-starvation therapy. The nanosystem excitingly improves tumor penetration and cellular uptake efficiency via integrating the pH-responsive size reduction and charge reversal and ROS-sensitive MOF disintegration and drug release strategy. Furthermore, the degradation of MOF and cargoes release can be self-amplified via additional self-generation H2 O2 mediated by GOD. Last, the released GOD and BPTES collaboratively cut off the energy supply of tumors and induce significant mitochondrial damage and cell cycle arrest via simultaneous restriction of glycolysis and compensatory glutamine metabolism pathways, consequently realizing the remarkable triple negative breast cancer killing effect in vivo with good biosafety via the dual starvation therapy.
Assuntos
Estruturas Metalorgânicas , Neoplasias , Humanos , Estruturas Metalorgânicas/farmacologia , Glutamina/metabolismo , Glutamina/uso terapêutico , Espécies Reativas de Oxigênio , Glucose , Neoplasias/tratamento farmacológico , Neoplasias/metabolismo , Nanotecnologia , Glucose Oxidase/metabolismo , Linhagem Celular Tumoral , Microambiente TumoralRESUMO
Tumor microenvironment is characterized by the high concentration of reactive oxygen species (ROS), which is an effective key used to open the Pandora's Box against cancer. Herein, a tumor-targeted nanosystem HFNP@GOX@PFC composed of ROS-cleaved Fe-based metal-organic framework, hyaluronic acid (HA), glucose oxidase (GOX) and perfluorohexane (PFC) has been developed for tumor cascade amplified starvation and chemodynamic therapy (CDT). In response to the high concentration of hydrogen peroxide (H2O2) intratumorally, HFNP@GOX@PFC endocytosed by tumor cells can specially be disassembled and release GOX, PFC and Fe2+, which can collectively starve tumor and self-produce additional H2O2 via competitively glucose catalyzing, supply oxygen to continuous support GOX-mediated starvation therapy, initiate CDT and cascade amplify oxidative stress via Fe2+-mediated Fenton reaction, leading to the serious tumor damage with activated p53 signal pathway. Moreover, HFNP@GOX@PFC also significantly initiates antitumor immune response via re-educating tumor-associated macrophages (TAMs) by activating NF-κB and MAPK signal pathways. In vitro and in vivo results collectively demonstrate that nanosystem not only continuously initiates starvation therapy, but also pronouncedly cascade-amplify CDT and polarize TAMs, consequently efficiently inhibiting tumor growth with good biosafety. The functional nanosystem combined the cascade amplification of starvation and CDT provides a new nanoplatform for tumor therapy.
Assuntos
Inanição , Macrófagos Associados a Tumor , Humanos , Peróxido de Hidrogênio , Espécies Reativas de Oxigênio , Endocitose , Glucose , Glucose OxidaseRESUMO
The starvation therapy mediated by the lonidamine (LND) was limited by the low drug delivery efficiency, off-target effect and compensative glutamine metabolism. Herein, a hyaluronic acid (HA)-modified reduction-responsive micellar nanosystem co-loaded with glycolysis and glutamine metabolism inhibitor (LND and bis-2-(5-phenylacetmido-1,2,4-thiadiazol-2-yl)ethyl sulfide, BPTES) was constructed for tumor-targeted dual-starvation therapy. The in vitro and in vivo results collectively suggested that the fabricated nanosystem could effectively endocytosed by tumor cells via HA receptor-ligand recognition, and rapidly release starvation-inducers LND and BPTES in response to the GSH-rich intratumoral cytoplasm. Furthermore, the released LND and BPTES were capable of inducing glycolysis and glutamine metabolism suppression, and accompanied by significant mitochondrial damage, cell cycle arrest and tumor cells apoptosis, eventually devoting to the blockade of the energy and substance supply and tumor killing with high efficiency. In summary, HPPPH@L@B nanosystem significantly inhibited the compensatory glycolysis and glutamine metabolism via the dual-starvation therapy strategy, blocked the indispensable energy and substance supply of tumors, consequently leading to the desired tumor starvation and effective tumor killing with reliable biosafety.
RESUMO
The classical AlCl3/imidazole-chloride-salt ionic liquid electrolytes are expensive, corrosive, and environmentally sensitive, which limit the large-scale application of aluminum-ion batteries. Herein, a gel polymer electrolyte is prepared through a facile process using a low-cost AlCl3/Et3NHCl ionic liquid as the plasticizer and polyamide as the polymer matrix. The gel polymer electrolyte achieves a decent ionic conductivity of 3.86 × 10-3 S cm-1, a wide electrochemical stability window of 2.6 V (vs Al), and long-term interfacial stability at room temperature. The assembled Al//graphite battery delivers considerable rate capability and excellent cycling performance. Besides, the gel polymer electrolyte can alleviate both moisture sensitivity and leakage corrosion issues owing to the full encapsulation of the ionic liquid by polyamide polymeric matrix. The gel polymer electrolyte should offer great potential for aluminum-ion battery applications.
RESUMO
Graphitic multi-walled carbon nanotubes (MWCNTs) can function as high-performance cathode materials for rechargeable Al-ion batteries with well-defined discharging plateaus and reasonable charge/discharge C-rates. However, the main intercalation/deintercalation or adsorption/desorption path of AlCl4- anions into or onto G-MWCNTs has not been elucidated. Herein, we used battery cells comprised of G-MWCNTs with different aspect ratios, Al metal, and AlCl3/1-ethyl-3-methylimidazolium chloride ionic liquid as the cathode, anode, and electrolyte, respectively. The electrochemical performance of the Al||G-MWCNT cell increased as the aspect ratio of the G-MWCNT cathode increased (i. e., longer and thinner). The degree of defects of the G-MWCNTs was similar (0.15-0.22); hence, the results confirm that the main and alternate paths for the AlCl4- intercalation/de-intercalation or adsorption/desorption into/from or onto/from the G-MWCNT are the basal and edge planes, respectively. The step-like structures of defects on the basal plane provide the main reaction site for AlCl4- anions.
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Notorious lithium dendrite causes severe capacity fade and harsh safety issues of lithium metal batteries, which hinder the practical applications of lithium metal electrodes in higher energy rechargeable batteries. Here, a kind of 3D-cross-linked composite network is successfully employed as a flexible-rigid coupling protective layer on a lithium metal electrode. During the plating/stripping process, the composite protective layer would enable uniform distribution of lithium ions in the adjacent regions of the lithium electrode, resulting in a dendrite-free deposition at a current density of 2 mA cm-2 . The LiNi0.5 Mn1.5 O4 -based lithium metal battery presents an excellent cycling stability at a voltage range of 3.5-5.0 V with the induction of 3D-cross-linked composite protective layer. From an industrial field application of view, thin lithium metal electrodes (40 µm, with 4 times excess lithium) can be used in LiNi0.5 Mn1.5 O4 (with industrially significant loading of 18 mg cm-2 and 2.6 mAh cm-2 )-based lithium metal batteries, which reveals a promising opportunity for practical applicability in high energy lithium metal batteries.
RESUMO
Ischemic stroke is a leading cause of death and permanent disability worldwide. Middle cerebral artery occlusion (MCAO) of variable duration times could be anticipated to result in varying degrees of injury that evolve spatially over time. Therefore, investigations following strokes require information concerning the spatiotemporal dimensions of the ischemic core as well as of perilesional areas. In the present study, multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) was applied to image such pathophysiological events. The ischemic time-points for evaluation were set at 6, 24, 48, and 72 hours after MCAO. Our results demonstrated that MPM has the ability to not only identify the normal and ischemic brain regions, but also reveal morphological changes of the cortex and striatum at various times following permanent MCAO. These findings corresponded well with the hematoxylin and eosin (H&E) stained tissue images. With the technologic progression of miniaturized imaging devices, MPM can be developed into an effective diagnostic and monitoring tool for ischemic stroke.
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A new salt of lithium trifluoro(perfluoro-tert-butyloxyl)borate (LiTFPFB) which possesses a bulky fluoroalkoxyl functional group in the borate anion has been synthesized for high energy lithium metal batteries. The presence of the bulky fluoroalkoxyl group in the borate anion of LiTFPFB can facilitate ion dissociation and in situ generate a protective film on the Li anode. As a result, LiTFPFB possesses a dramatically improved ionic conductivity and LiFePO4/Li cells using 1.0 M LiTFPFB/PC electrolyte exhibit improved capacity retention especially upon cycling at elevated temperature (60 °C). Ex situ surface analysis reveals that a protective film is formed on the lithium metal anode, which can inhibit further decomposition of the electrolyte. Furthermore, the LiTFPFB based electrolyte also imparts an excellent cycling performance to LiCoO2/Li metal cells for 500 cycles. The outstanding performance of the LiTFPFB salt demonstrates that it is a very promising baseline salt for next generation lithium metal batteries.
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Organic electrodes are potential alternatives to current inorganic electrode materials for lithium ion and sodium ion batteries powering portable and wearable electronics, in terms of their mechanical flexibility, function tunability and low cost. However, the low capacity, poor rate performance and rapid capacity degradation impede their practical application. Here, we concentrate on the molecular design for improved conductivity and capacity, and favorable bulk ion transport. Through an in situ cross-coupling reaction of triethynylbenzene on copper foil, the carbon-rich frame hydrogen substituted graphdiyne film is fabricated. The organic film can act as free-standing flexible electrode for both lithium ion and sodium ion batteries, and large reversible capacities of 1050 mAh g-1 for lithium ion batteries and 650 mAh g-1 for sodium ion batteries are achieved. The electrode also shows a superior rate and cycle performances owing to the extended π-conjugated system, and the hierarchical pore bulk with large surface area.
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Novel sulfur cathodes hold the key to the development of metal-sulfur batteries, the promising candidate of next-generation high-energy-storage systems. Herein, a fascinating sulfur cathode based on sulfide graphdiyne (SGDY) is designed with a unique structure, which is composed of a conducting carbon skeleton with high Li+ mobility and short sulfur energy-storing unites. The SGDY cathode can essentially avoid polysulfide dissolution and be compatible with commercially available carbonate-based electrolytes and Grignard reagent-based electrolytes (all phenyl complex (APC) type electrolytes). Both the assembled Li-S and Mg-S batteries exhibit excellent electrochemical performances including large capacity, superior rate capability, high capacity retention, and high Coulombic efficiency. More importantly, this is the first implementation case of a reliable Mg-S system based on nucleophilic APC electrolytes.
RESUMO
Redox mediators (RMs) have been widely applied to reduce the charge overpotential of nonaqueous lithium-oxygen (Li-O2) batteries. Among the reported RMs, LiI is under hot debate with lots of controversial reports. However, there is a limited understanding of the charge mechanism of I- in anhydrous Li-O2 batteries. Here, we study the chemical reactivity between the oxidized state of I- and Li2O2. We confirm that the Li2O2 particles could be chemically oxidized by I2 rather than I3- species. Furthermore, our work demonstrates that the generated I- from Li2O2 oxidation would combine with I2 to give I3- species, hindering further oxidation of Li2O2 by I2. To improve the working efficiency of I- RMs, we introduce a compound LiI(3-hydroxypropionitrile)2 (LiI(HPN)2) with a high binding ability of I-. Compared with LiI, the cell that contained LiI(HPN)2 shows a significantly increased amount of I2 species during charge and enhanced Li2O2 oxidation efficiency under the same working conditions.
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The cerebellum is the prominent laminar structure of the mammalian brain that has been implicated in various psychiatric and neurological diseases. Although clinical brain imaging techniques have provided precise anatomic images of cerebellar structures, a definitive diagnosis still requires adequate resolution to identify individual layers in cerebellar cortex, the extent of tumor, even requires the histological tissue examination during surgical procedures. In this study, multiphoton microscopy (MPM), based on second harmonic generation (SHG) and two-photon excited fluorescence (TPEF), was perform on the rat cerebellar structures and pathology with the combination of image analysis methods. Results show that MPM can reveal the cerebellar vermis, hemispheres, medulla, and ventricle, as well as axon bundles, Purkinje cells, capillaries, and the pia mater of the cerebellum. Together with custom-developed image processing algorithms, MPM could further differentiate between the gray and white matter, as well as evaluate the Purkinje cell layer, identify the cerebellar tumor boundary, and distinguish between the tumor core and peritumor regions. Our results establish a direct visualization and rapid assessment approach for the cerebellar structures, as well as suggest the feasibility of inâ vivo multiphoton microendoscopes and fiberscopes as clinical tools for neuropathological diagnoses.
Assuntos
Cerebelo/citologia , Cerebelo/diagnóstico por imagem , Microscopia de Fluorescência por Excitação Multifotônica/métodos , Animais , Doenças Cerebelares/diagnóstico por imagem , Doenças Cerebelares/patologia , Neoplasias Cerebelares/diagnóstico por imagem , Neoplasias Cerebelares/patologia , Cerebelo/patologia , Substância Cinzenta/citologia , Substância Cinzenta/diagnóstico por imagem , Substância Cinzenta/patologia , Hemangioblastoma/diagnóstico por imagem , Hemangioblastoma/patologia , Humanos , Células de Purkinje/citologia , Células de Purkinje/patologia , Ratos , Ratos Sprague-Dawley , Fatores de Tempo , Substância Branca/citologia , Substância Branca/diagnóstico por imagem , Substância Branca/patologiaRESUMO
Polycyanoacrylate is a very promising matrix for polymer electrolyte, which possesses advantages of strong binding and high electrochemical stability owing to the functional nitrile groups. Herein, a facile and reliable in situ polymerization strategy of poly(ethyl cyanoacrylate) (PECA) based gel polymer electrolytes (GPE) via a high efficient anionic polymerization was introduced consisting of PECA and 4 M LiClO4 in carbonate solvents. The in situ polymerized PECA gel polymer electrolyte achieved an excellent ionic conductivity (2.7 × 10-3 S cm-1) at room temperature, and exhibited a considerable electrochemical stability window up to 4.8 V vs Li/Li+. The LiFePO4/PECA-GPE/Li and LiNi1.5Mn0.5O4/PECA-GPE/Li batteries using this in-situ-polymerized GPE delivered stable charge/discharge profiles, considerable rate capability, and excellent cycling performance. These results demonstrated this reliable in situ polymerization process is a very promising strategy to prepare high performance polymer electrolytes for flexible thin-film batteries, micropower lithium batteries, and deformable lithium batteries for special purpose.
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The elemental N emerged uniformly in graphdiyne (GDY) after heat treatment under NH3 atmosphere to form N-doping GDY. The interplanar N-GDY distance decreased slightly, which may be ascribed to the smaller atom radius of N than C. Compared with GDY, the introduction of N atoms in N-GDY created numerous heteroatomic defects and active sites, thus achieving enhanced electrochemical properties, including higher reversible capacity, improved rate performance, and superior cycling stability. In addition, N-doping might be advantageous to minimize the surface side reactions and form stable interfaces, hence improving the electrochemical cycling stability of N-GDY electrodes. These results indicate N-doping is also an efficient way for improving the electrochemical performance of GDY materials.
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Nickel disulfide-graphene nanosheets (NiS2-GNS) composites were successfully synthesized via a simple and mild hydrothermal method. It was revealed by scanning electron microscopy and transmission electron microscopy images that the spherical NiS2 nanoparticles with a diameter of 200-300 nm were uniformly dispersed on graphene nanosheets. Na(+) electrochemical storage properties including cycling performance and high-rate capability of NiS2-GNS composites were investigated, demonstrating a superior reversible capacity of 407 mAh g(-1) with the capacity retention of 77% over 200 cycles at a current density of 0.1 C. Furthermore, even at a large current density of 2 C, a high capacity of 168 mAh g(-1) can still remain, which is much higher than that of pristine NiS2 materials. The enhancement in electrochemical properties might be attributed to the synergetic effect endowed by high conductivity of graphene and novel structure of the electrode material. Combined with the advantages of low cost and environmental benignity, NiS2-GNS composite would be a potential anode material for sodium ion batteries.
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Self-supported Ni3S2 ultrathin nanosheets were in situ formed by direct sulfurization of commercially available nickel foam using thioacetamide as sulfur source under hydrothermal process. The morphology and structure of the as-obtained sample were analyzed by using XRD, XPS, SEM, and TEM, revealing that an ultrathin nanosheets Ni3S2 were grown on the surface of Ni form. The as-obtained Ni3S2/Ni composite with uniform architecture was used as cathode material for alkaline Ni/Zn battery, which delivered high capacity of 125 mAh g(-1) after 100 cycles with no obvious capacity fading, extraordinary rate capability (68 mAh g(-1) at the current density of 5.0 A g(-1)), and high operating voltage (1.75 V).
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Primary vaginal cancer is not common, representing 1-2% of all female genital malignancies. We present a case of a third-degree uterine prolapse complicated by an isolated primary vaginal cancer and its surgical treatment. The cervix was clinically normal, but on the nearby prolapsed vaginal wall, a large exogenous hard lesion had developed. A biopsy of the lesion revealed a squamous carcinoma. The patient was asymptomatic and had no recurrence during the last 4 years of follow-up after surgical treatment with radiotherapy. The surgical treatment with or without radiotherapy is the optimum treatment for uterine prolapse with early-stage vaginal squamous cell carcinoma, although the majority of vaginal malignancies are treated with radiotherapy. We recommend always performing a biopsy prior to surgery in prolapse-induced ulceration.