Regeneratable bioinspired nanochannels for highly sensitive electrochemical detection of glycated albumin.

Glycated albumin (GA) has been proposed as a reliable diabetes mellitus marker particularly useful in assessing intermediate glycemic control. Herein, we designed a bioinspired nanochannels for biochemical detection based on the host-guest interaction between ß-cyclodextrin and azobenzene. Cyclodextrin was grafted on the inner surface of nanochannels of a nanoporous membrane and azobenzene was tagged to the terminal of GA aptamer, thereby facilitating the orientation of GA aptamer in the nanochannels. The presence of GA was monitored by recording the voltammetric signal of ferricyanide that transported across the nanochannel array. The peak current exhibited a linearity relationship with the GA concentration across a broad range of 1.0 ng mL-1 to 100 µg mL-1, along with a detection limit of 0.18 ng mL-1. Notably, the aptamer could be offloaded under ultraviolet light, regenerating the cyclodextrin functionalized nanochannels for subsequent re-immobilization of the fresh aptamer. The relative standard deviation for seven cycles of regeneration treatment was no more than 1.8 %. The remarkable reusability of the nanochannels offered a cost-effective, sensitive and reproducible aptasensing platform.

Nationwide meta-analysis of microplastic distribution and risk assessment in China's aquatic ecosystems, soils, and sediments.

Microplastic (MP) accumulation has recently become a pressing global environmental challenge. As a major producer and consumer of plastic products, China's MP pollution has garnered significant attention from researchers. However, accurate and comprehensive investigations of national-level MP pollution are still lacking. In this study, we systematically collated a national MP pollution dataset consisting of 7766 water, soil, and sediment sampling sites from 544 publicly published studies, revealing the spatiotemporal distribution and potential risks of MP pollution in China. The results indicate that MP distribution is influenced by various regional factors, including economic development level, population distribution, and geographical environment, exhibiting considerable range and complexity. MP concentrations are generally higher in economically prosperous areas, but the degree of pollution varies significantly across different environmental media. Given the uncertainty and lack of standardized data in traditional microplastic risk assessment methods, this article highlights the urgency of developing a comprehensive big data and artificial intelligence (AI)-based regulatory framework. This work provides a substantial amount of accurate MP pollution data and offers a fresh perspective on leveraging AI for microplastic pollution regulation.

A novel method for multi-pollutant monitoring in water supply systems using chemical machine vision.

Drinking water is vital for human health and life, but detecting multiple contaminants in it is challenging. Traditional testing methods are both time-consuming and labor-intensive, lacking the ability to capture abrupt changes in water quality over brief intervals. This paper proposes a direct analysis and rapid detection method of three indicators of arsenic, cadmium, and selenium in complex drinking water systems by combining a novel long-path spectral imager with machine learning models. Our technique can obtain multiple parameters in about 1 s. The experiment involved setting up samples from various drinking water backgrounds and mixed groups, totaling 9360 injections. A raw visible light source ranging from 380 to 780 nm was utilized, uniformly dispersing light into the sample cell through a filter. The residual beam was captured by a high-definition camera, forming a distinctive spectrum. Three deep learning models-ResNet-50, SqueezeNet V1.1, and GoogLeNet Inception V1-were employed. Datasets were divided into training, validation, and test sets in a 6:2:2 ratio, and prediction performance across different datasets was assessed using the coefficient of determination and root mean square error. The experimental results show that a well-trained machine learning model can extract a lot of feature image information and quickly predict multi-dimensional drinking water indicators with almost no preprocessing. The model's prediction performance is stable under different background drinking water systems. The method is accurate, efficient, and real-time and can be widely used in actual water supply systems. This study can improve the efficiency of water quality monitoring and treatment in water supply systems, and the method's potential for environmental monitoring, food safety, industrial testing, and other fields can be further explored in the future.

Self-Assembly of Heterogeneous Ferritin Nanocages for Tumor Uptake and Penetration.

Well-defined nanostructures are crucial for precisely understanding nano-bio interactions. However, nanoparticles (NPs) fabricated through conventional synthesis approaches often lack poor controllability and reproducibility. Herein, a synthetic biology-based strategy is introduced to fabricate uniformly reproducible protein-based NPs, achieving precise control over heterogeneous components of the NPs. Specifically, a ferritin assembly toolbox system is developed that enables intracellular assembly of ferritin subunits/variants in Escherichia coli. Using this strategy, a proof-of-concept study is provided to explore the interplay between ligand density of NPs and their tumor targets/penetration. Various ferritin hybrid nanocages (FHn) containing human ferritin heavy chains (FH) and light chains are accurately assembled, leveraging their intrinsic binding with tumor cells and prolonged circulation time in blood, respectively. Further studies reveal that tumor cell uptake is FH density-dependent through active binding with transferrin receptor 1, whereas in vivo tumor accumulation and tissue penetration are found to be correlated to heterogeneous assembly of FHn and vascular permeability of tumors. Densities of 3.7 FH/100 nm2 on the nanoparticle surface exhibit the highest degree of tumor accumulation and penetration, particularly in tumors with high permeability compared to those with low permeability. This study underscores the significance of nanoparticle heterogeneity in determining particle fate in biological systems.

Freshwater crustacean exposed to active pharmaceutical ingredients: ecotoxicological effects and mechanisms.

Concerns over the ecotoxicological effects of active pharmaceutical ingredients (APIs) on aquatic invertebrates have been raised in the last decade. While numerous studies have reported the toxicity of APIs in invertebrates, no attempt has been made to synthesize and interpret this dataset in terms of different exposure scenarios (acute, chronic, multigenerational), multiple crustacean species, and the toxic mechanisms. In this study, a thorough literature review was performed to summarize the ecotoxicological data of APIs tested on a range of invertebrates. Therapeutic classes including antidepressants, anti-infectives, antineoplastic agents, hormonal contraceptives, immunosuppressants, and neuro-active drugs exhibited higher toxicity to crustaceans than other API groups. The species sensitivity towards APIs exposure is compared in D. magna and other crustacean species. In the case of acute and chronic bioassays, ecotoxicological studies mainly focus on the apical endpoints including growth and reproduction, whereas sex ratio and molting frequency are commonly used for evaluating the substances with endocrine-disrupting properties. The multigenerational and "Omics" studies, primarily transcriptomics and metabolomics, were confined to a few API groups including beta-blocking agents, blood lipid-lowing agents, neuroactive agents, anticancer drugs, and synthetic hormones. We emphasize that in-depth studies on the multigenerational effects and the toxic mechanisms of APIs on the endocrine systems of freshwater crustacean are warranted.

A spectral learning path for simultaneous multi-parameter detection of water quality.

Water quality parameters (WQP) are the most intuitive indicators of the environmental quality of water body. Due to the complexity and variability of the chemical environment of water body, simple and rapid detection of multiple parameters of water quality becomes a difficult task. In this paper, spectral images (named SPIs) and deep learning (DL) techniques were combined to construct an intelligent method for WQP detection. A novel spectroscopic instrument was used to obtain SPIs, which were converted into feature images of water chemistry and then combined with deep convolutional neural networks (CNNs) to train models and predict WQP. The results showed that the method of combining SPIs and DL has high accuracy and stability, and good prediction results with average relative error of each parameter (anions and cations, TOC, TP, TN, NO3--N, NH3-N) at 1.3%, coefficient of determination (R2) of 0.996, root mean square error (RMSE) of 0.1, residual prediction deviation (RPD) of 16.2, and mean absolute error (MAE) of 0.067. The method can achieve rapid and accurate detection of high-dimensional water quality multi-parameters, and has the advantages of simple pre-processing and low cost. It can be applied not only to the intelligent detection of environmental waters, but also has the potential to be applied in chemical, biological and medical fields.

The APC/CTAD1-WIDE LEAF 1-NARROW LEAF 1 pathway controls leaf width in rice.

Leaf morphology is one of the most important features of the ideal plant architecture. However, the genetic and molecular mechanisms controlling this feature in crops remain largely unknown. Here, we characterized the rice (Oryza sativa) wide leaf 1 (wl1) mutant, which has wider leaves than the wild-type due to more vascular bundles and greater distance between small vascular bundles. WL1 encodes a Cys-2/His-2-type zinc finger protein that interacts with Tillering and Dwarf 1 (TAD1), a co-activator of the anaphase-promoting complex/cyclosome (APC/C) (a multi-subunit E3 ligase). The APC/CTAD1 complex degrades WL1 via the ubiquitin-26S proteasome degradation pathway. Loss-of-function of TAD1 resulted in plants with narrow leaves due to reduced vascular bundle numbers and distance between the small vascular bundles. Interestingly, we found that WL1 negatively regulated the expression of a narrow leaf gene, NARROW LEAF 1 (NAL1), by recruiting the co-repressor TOPLESS-RELATED PROTEIN and directly binding to the NAL1 regulatory region to inhibit its expression by reducing the chromatin histone acetylation. Furthermore, biochemical and genetic analyses revealed that TAD1, WL1, and NAL1 operated in a common pathway to control the leaf width. Our study establishes an important framework for understanding the APC/CTAD1-WL1-NAL1 pathway-mediated control of leaf width in rice, and provides insights for improving crop plant architecture.

Image learning to accurately identify complex mixture components.

The study of complex mixtures is very important for exploring the evolution of natural phenomena, but the complexity of the mixtures greatly increases the difficulty of material information extraction. Image perception-based machine-learning techniques have the ability to cope with this problem in a data-driven way. Herein, we report a 2D-spectral imaging method to collect matter information from mixture components, and the obtained feature images can be easily provided to deep convolutional neural networks (CNNs) for establishing a spectral network. The results demonstrated that a single CNN trained end-to-end from the proposed images can directly accomplish synchronous measurement of multi-component samples using only raw pixels as inputs. Our strategy has some innate advantages, such as fast data acquisition, low cost, and simple chemical treatment, suggesting that it can be extensively applied in many fields, including environmental science, biology, medicine, and chemistry.

LF1 regulates the lateral organs polarity development in rice.

The patterning of adaxial-abaxial tissues plays a vital role in the morphology of lateral organs, which is maintained by antagonism between the genes that specify adaxial and abaxial tissue identity. The homeo-domain leucine zipper class III (HD-ZIP III) family genes regulate adaxial identity; however, little information is known about the physical interactions or transcriptionally regulated downstream genes of HD-ZIP III. In this study, we identified a dominant rice mutant, lateral floret 1 (lf1), which has defects in lateral organ polarity. LF1 encodes the HD-ZIP III transcription factor, which expressed in the adaxial area of lateral organs. LF1 can activate directly the expression of LITTLE ZIPPER family gene OsZPR4 and HD-ZIP II family gene OsHOX1, and OsZPR4 and OsHOX1 respectively interact with LF1 to form a heterodimer to repress the transcriptional activity of LF1. LF1 influences indole-3-acetic acid (IAA) content by directly regulating the expression of OsYUCCA6. Therefore, LF1 forms negative feedback loops between OsZPR4 and OsHOX1 to affect IAA content, leading to the regulation of lateral organs polarity development. These results reveal the cross-talk among HD-ZIP III, LITTLE ZIPPER, and HD-ZIP II proteins and provide new insights into the molecular mechanisms underlying the polarity development of lateral organs.

Machine learning based on holographic scattering spectrum for mixed pollutants analysis.

Determination of complex pollutants often involves many high-cost and laborious operations. Today's pop machine-learning (ML) technology has exhibited their amazing successes in image recognition, drug designing, disease detection, natural language understanding, etc. ML-driven samples testing will inevitably promote the development of related subjects and fields, but the biggest challenge ahead for this process is how to provide some intelligible and sufficient data for various algorithms. In this work, we present a full strategy for rapid detecting mixed pollutants through the synergistic application of holographic spectrum and convolutional neural network (CNN). The results have shown that a well-trained CNN model could realize quantitative analysis of the mixed pollutants by extracting spectral information of matters, suggesting the strategy's value in facilitating the study of complex chemical systems.

Machine learning for total organic carbon analysis of environmental water samples using high-throughput colorimetric sensors.

Due to the complexity of nonlinear reactions, the analysis of environmental samples often relies on expensive equipment as well as tedious and time-consuming experimental procedures. Currently, the efficient machine learning (ML) strategy based on big data offers some new insights for the analysis of complex components in the environmental field. In this study, ML was applied for the analysis of total organic carbon (TOC). We prepared a special colorimetric sensor (c-sensor) by inkjet printing. The sensor reacted with water samples in a high-throughput process, producing characteristic patterns to map TOC information in water samples. To quickly acquire TOC information on c-sensors, a ML model was proposed to describe the relationship between the c-sensor and TOC value. According to this study, the c-sensor and ML can be effectively applied to TOC information analysis of environmental water samples, which provides convenience for environmental research. It is foreseeable that ML has a broad prospect of application in environmental research.

Machine learning for mixture toxicity analysis based on high-throughput printing technology.

Analysis on mixture toxicity (Mix-tox) of the multi-chemical space is constantly followed with interest for many researchers. Conventional toxicity tests with time-consuming and costly operations make researchers can only establish some toxicity prediction models aiming to a limited sampling dimension. The rapid development of machine learning (ML) algorithm will accelerate the exploration of many fields involving toxicity analysis. Rather than the model calculation capacity, the challenge of this process mainly comes from the lack of toxicology big-data to perform toxicity perception through the ML model. In this paper, a full strategy based a standardized high-throughput experiment was developed for Mix-tox analysis throughout the whole routine, from big-sample dataset design, model building, and training, to the toxicity prediction. Using the concentration variates as input and bio-luminescent inhibition rate as output, it turned out that a well-trained random forest algorithm was successfully applied to assess the mixtures' toxicity effect, suggesting its value in facilitating adoption of Mix-tox analysis.

A color-spectral machine learning path for analysis of five mixed amino acids.

A machine learning (ML) strategy based on color-spectral images for mixed amino acid (AA) analysis is presented. The results showed that a well-trained ML model could accurately predict multiple AAs at the same time, suggesting its value for facilitating quantitative analysis of mixed AA systems.

A review of soil heavy metal pollution from industrial and agricultural regions in China: Pollution and risk assessment.

Soil heavy metal pollution has been becoming serious and widespread in China. To date, there are few studies assessing the nationwide soil heavy metal pollution induced by industrial and agricultural activities in China. This review obtained heavy metal concentrations in soils of 402 industrial sites and 1041 agricultural sites in China throughout the document retrieval. Based on the database, this review assessed soil heavy metal concentration and estimated the ecological and health risks on a national scale. The results revealed that heavy metal pollution and associated risks posed by cadmium (Cd), lead (Pb) and arsenic (As) are more serious. Besides, heavy metal pollution and associated risks in industrial regions are severer than those in agricultural regions, meanwhile, those in southeast China are severer than those in northwest China. It is worth noting that children are more likely to be affected by heavy metal pollution than adults. Based on the assessment results, Cd, Pb and As are determined as the priority control heavy metals; mining areas are the priority control areas compared to other areas in industrial regions; food crop plantations are the priority control areas in agricultural regions; and children are determined as the priority protection population group. This paper provides a comprehensive ecological and health risk assessment on the heavy metals in soils in Chinese industrial and agricultural regions and thus provides insights for the policymakers regarding exposure reduction and management.

Preparation and catalytic performance of copper-containing magnetic catalysts for degradation of azo dye (direct violet).

A novel magnetically separable magnetic activated carbon supporting-copper (MCAC) catalyst for catalytic wet peroxide oxidation (CWPO) was prepared by chemical impregnation. The prepared samples were characterized by X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) method, and scanning electron microscopy (SEM) equipped with energy dispersive spectrometry (EDS). The catalytic performance of the catalysts was evaluated by direct violet (D-BL) degradation in CWPO experiments. The influence of preparative and operational parameters (dipping conditions, calcination temperature, catalyst loading H2O2 dosage, pH, reaction temperature, additive salt ions and initial D-BL concentration) on degradation performance of CWPO process was investigated. The resulting MCAC catalyst showed higher reusability in direct violet oxidation than the magnetic activated carbon (MAC). Besides, dynamic tests also showed the maximal degradation rate reached 90.16% and its general decoloring ability of MCAC was 34 mg g-1 for aqueous D-BL.

Distribution of Heavy Metal Pollution in Surface Soil Samples in China: A Graphical Review.

Soil pollution in China is one of most wide and severe in the world. Although environmental researchers are well aware of the acuteness of soil pollution in China, a precise and comprehensive mapping system of soil pollution has never been released. By compiling, integrating and processing nearly a decade of soil pollution data, we have created cornerstone maps that illustrate the distribution and concentration of cadmium, lead, zinc, arsenic, copper and chromium in surficial soil across the nation. These summarized maps and the integrated data provide precise geographic coordinates and heavy metal concentrations; they are also the first ones to provide such thorough and comprehensive details about heavy metal soil pollution in China. In this study, we focus on some of the most polluted areas to illustrate the severity of this pressing environmental problem and demonstrate that most developed and populous areas have been subjected to heavy metal pollution.

Fluorescence imaging technology (FI) for high-throughput screening of selenide-modified nano-TiO2 catalysts.

A high-throughput screening (HTS) method based on fluorescence imaging (FI) was implemented to evaluate the catalytic performance of selenide-modified nano-TiO2. Chemical ink-jet printing (IJP) technology was reformed to fabricate a catalyst library comprising 1405 (Ni(a)Cu(b)Cd(c)Ce(d)In(e)Y(f))Se(x)/TiO2 (M6Se/Ti) composite photocatalysts. Nineteen M6Se/Tis were screened out from the 1405 candidates efficiently.