Mixed-Method Precooling Enhances Self-Paced 20-km Cycling Time-Trial Performance When Apparent Temperature Is >46 °C but May Not Be a Priority in <46 °C.

PURPOSE: Precooling (PreC) may only benefit performance when thermal strain experienced by an individual is sufficiently high. We explored the effect of mixed-method PreC on 20-km cycling time-trial (CTT) performance under 3 different apparent temperatures (AT). METHODS: On separate days, 12 trained or highly trained male cyclists/triathletes completed six 20-km CTTs in 3 different ATs: hot-dry (35 °C AT), moderately hot-humid (40 °C AT), and hot-humid (46 °C AT). All trials were preceded by 30 minutes of mixed-method PreC or no PreC (control [CON]). RESULTS: Faster 2.5-km-split completion times occurred in PreC compared with CON in 46 °C AT (P = .02), but not in 40 °C AT (P = .62) or 35 °C AT (P = .57). PreC did not affect rectal and body temperature during the 20-km CTT. Skin temperature was lower throughout the CTT in PreC compared with CON in 46 °C AT (P = .01), but not in 40 °C AT (P = 1.00) and 35 °C AT (P = 1.00). Heart rate had a greater rate of increase during the CTT for PreC compared with CON in 46 °C AT (P = .01), but not in 40 °C AT (P = .57) and 35 °C AT (P = 1.00). Ratings of perceived exertion (P < .001) and thermal comfort (P = .04) were lower for PreC compared with CON in 46 °C AT only, while thermal sensation was not different between PreC and CON. CONCLUSION: Mixed-method PreC should be applied prior to 20-km CTTs conducted in hot-humid conditions (≥46 °C AT). Alternatively, mixed-method PreC may be a priority in moderately hot-humid (∼40 °C AT) conditions but should not be in hot-dry (∼35 °C AT) conditions for 20-km CTT.

Intraindividual variability in sleep among athletes: A systematic review of definitions, operationalizations, and key correlates.

Via systematic review with narrative synthesis of findings, we aimed to document the ways by which researchers have defined, operationalized, and examined sleep variability among athletes. We identified studies in which scholars examined intraperson variability in sleep among athletes via a search of six databases (Web of Science, Embase, Medline, PsycINFO, CINHAL Plus, and ProQuest Dissertations and Theses Global) using a protocol that included keywords for the target outcome (sleep*), population (athlet* OR sport*), and outcome operationalization (variability OR variation OR "standard deviation" OR fluctuate OR fluctuation OR stability OR instability OR reactivity OR IIV OR intraindividual). We complemented this primary search with citation searching of eligible articles. Assessments of study quality captured eight core elements, namely aims/hypotheses, sample size justification, sample representativeness, number of days sleep assessed, measures of sleep and its correlates, missing data, and inferences and conclusions. From a total of 1209 potentially relevant papers, we identified 16 studies as meeting our eligibility criteria. Concept definitions of variability were notably absent from this work and where available were vague. Quantitative deviations from one's typical level of target sleep metrics reflected the essence by which all but one of the research teams operationalized sleep variability. We assessed the overall quality of empirical work as moderate in nature. We propose a working definition of sleep variability that can inform knowledge generation on the temporal, day-to-day dynamics of sleep functioning that is required for personalized interventions for optimizing sleep health.

Acute exercise affects dual-energy X-ray absorptiometry body composition estimates but not standardised ultrasound measurements of subcutaneous adipose tissue.

Ultrasound has been demonstrated to be a highly accurate and reliable tool for measuring subcutaneous adipose tissue thickness and is robust against changes in hydration status or acute food or fluid intake. However, the effect of prior acute exercise is unexamined. This study examined the impact of an acute endurance exercise and resistance exercise session on standardised brightness-mode ultrasound measurements of subcutaneous adipose tissue thickness compared to skinfolds and dual-energy X-ray absorptiometry body composition estimates. In a randomised cross-over design, 30 active adults (24.2 ± 4.9 years) undertook physique assessment via standardised brightness-mode ultrasound, skinfolds and dual-energy X-ray absorptiometry before, immediately and 45 min after an acute endurance or resistance exercise session. The mean sum of eight subcutaneous adipose tissue thickness measured via standardised brightness-mode ultrasound increased (0.6 mm, p = 0.04) immediately postendurance exercise but was not meaningful when evaluated against the technical error of measurement of the investigator. A significant (p = 0.01) but not meaningful decrease in the sum of eight skinfolds occurred immediately (-1.1 ± 0.4 mm) and 45 min (-1.3 ± 0.4 mm) postresistance exercise. Comparatively, endurance exercise elicited a meaningful decrease of total mass (460 ± 30 g) and trunk lean mass (680 ± 90 g) dual-energy X-ray absorptiometry estimates. Findings from this study indicate standardised client presentation may be unnecessary when employing either standardised brightness-mode ultrasound or skinfolds for body composition assessment unlike dual-energy X-ray absorptiometry.

Food and fluid intake and hydration status does not affect ultrasound measurements of subcutaneous adipose tissue in active adults.

OBJECTIVES: To investigate the impact of acute food and fluid intake or hydration status on the standardised brightness-mode ultrasound measurement of subcutaneous adipose tissue thickness. DESIGN: Thirty active adults (female n = 10) participated in a randomised cross over study. METHODS: Participants completed three body composition assessment sessions via standardised brightness-mode ultrasound and Dual-energy X-ray absorptiometry. Participants were assessed under standardised presentation during 'food only' and 'food plus water' sessions at baseline and reassessed after their allotted intake. 'Hypohydration plus water' was undertaken in a hypohydrated state at baseline and reassessed after water intake. RESULTS: The sum of eight subcutaneous adipose tissue thickness was lower when measured after 'food only' or 'food plus water' compared to baseline (-0.1 to -0.9 mm; p < 0.01). However, these changes were less than the 95% confidence interval of the technical error of measurement of the investigator. Body mass, dual-energy x-ray absorptiometry total and trunk mass, lean mass and trunk lean mass estimates increased (p < 0.01) following 'food only' or 'food plus water', and decreased with hypohydration (p < 0.01). Total and regional fat mass estimates were not impacted. CONCLUSIONS: The sum of eight subcutaneous adipose tissue thickness measured via standardised brightness-mode ultrasound was unaffected by acute food and fluid consumption or hydration status changes. Comparatively, these interventions altered dual-energy x-ray absorptiometry body composition estimates, especially that of lean mass components. Standardised brightness-mode ultrasound can therefore be used to monitor changes in fat patterning when standardised client presentation is not practically achievable.

Measures of body composition via Dual-energy X-ray absorptiometry, ultrasound and skinfolds are not impacted by the menstrual cycle in active eumenorrheic females.

OBJECTIVES: (1) Compare changes in body composition estimates over the menstrual cycle in active females using Dual-energy X-ray absorptiometry, standardised brightness-mode ultrasound and skinfolds (2) Compare the predictability of Dual-energy X-ray absorptiometry fat mass estimate via standardised brightness-mode ultrasound versus skinfolds measurements. DESIGN: Thirty active females (27 ±â€¯5 y) with regularly occurring menstrual cycles participated in a cross sectional study. METHODS: Participants completed four assessment sessions scheduled according to each individual's menstrual cycle. These sessions took place during their (1) early follicular, (2) mid-to-late follicular, (3) mid-luteal and (4) second early follicular phases. Body composition estimates were acquired using Dual-energy X-ray absorptiometry, subcutaneous adipose tissue thickness was measured at eight sites using standardised brightness-mode ultrasound and skinfolds. RESULTS: The sum of eight subcutaneous adipose tissue thickness measured using standardised brightness-mode ultrasound and skinfolds were not different between the cycle phases (p > 0.05). Body mass and Dual-energy X-ray absorptiometry total mass estimate as well as Dual-energy X-ray absorptiometry estimates of total and regional lean and fat mass were also not different between cycle phases (p > 0.05) and any changes were within the 95% confidence intervals of their respective least significant change values. CONCLUSIONS: There were no true and meaningful changes in the sum of eight subcutaneous adipose tissue thickness measured via standardised brightness-mode ultrasound and skinfolds or Dual-energy X-ray absorptiometry total and regional tissue mass estimates across the menstrual cycle in active eumenorrheic females. Body composition may thus be assessed via these methods in this population at any cycle phase with standardised participant presentation.

Stress, physical activity, sedentary behavior, and resilience-The effects of naturalistic periods of elevated stress: A measurement-burst study.

Stress is an important consideration for understanding why individuals take part in limited or no physical activity. The negative effects of stress on physical activity do not hold for everyone, so examinations of possible resilience resources that might protect individuals from the harmful effects of stress are required. Accordingly, we conducted a measurement-burst study with 53 university students over a 6-month period to examine the dynamics among stress, physical activity, sedentary behavior, and resilience resources. Participants completed three bursts of 6 days, with each burst separated by an 8-week gap. Expectations regarding the moderating effects of resilience resources were unsupported. Daily reports of academic and general stress were positively associated with sedentary behavior and negatively associated with light and moderate intensity physical activity. Hair cortisol concentration significantly moderated the association between academic stress and sedentary behavior, such that in bursts where cortisol was lower the daily positive association between stress and sedentary behavior was weaker. The finding that academic and general stress are dynamically associated with lower levels of light and moderate intensity physical activity and higher levels of sedentary behavior is an important extension to previous research, which has relied mainly on cross-sectional designs and self-report methods. Future research might examine resilience resources that are specific to the outcomes of interest rather than rely on generic resources.

Vitamin D Supplementation Does Not Impact Resting Metabolic Rate, Body Composition and Strength in Vitamin D Sufficient Physically Active Adults.

Supplementation with the most efficient form of Vitamin D (VitD3) results in improvements in energy metabolism, muscle mass and strength in VitD deficient individuals. Whether similar outcomes occur in VitD sufficient individuals' remains to be elucidated. The aim of this study is to determine the effect of VitD3 supplementation on resting metabolic rate (RMR), body composition and strength in VitD sufficient physically active young adults. Participants completed pre-supplementation testing before being matched for sunlight exposure and randomly allocated in a counterbalanced manner to the VitD3 or placebo group. Following 12 weeks of 50 IU/kg body-mass VitD3 supplementation, participants repeated the pre-supplementation testing. Thirty-one adults completed the study (19 females and 12 males; mean ± standard deviation (SD); age = 26.6 ± 4.9 years; BMI = 24.2 ± 4.1 kg·m2). The VitD group increased serum total 25(OH)D by 30 nmol/L while the placebo group decreased total serum concentration by 21 nmol/L, reaching 123 (51) and 53 (42.2) nmol/L, respectively. There were no significant changes in muscle strength or power, resting metabolic rate and body composition over the 12-week period. Physically active young adults that are VitD sufficient have demonstrated that no additional physiological effects of achieving supraphysiological serum total 25(OH)D concentrations after VitD3 supplementation.

Cross-sectional and longitudinal comparisons of self-reported and device-assessed physical activity and sedentary behaviour.

OBJECTIVES: To examine the longitudinal associations and differences between self-reported and device-assessed physical activity (PA) and sedentary behaviour (SB), using a multifaceted statistical approach. DESIGN: Longitudinal measurement burst. METHODS: In total, 52 university students (78% female) aged 18-38 years (mean=21.94±4.57 years) participated. The study consisted of three blocks of six days of measurement, during which participants wore an accelerometer on their wrist for the entire block, and self-reported their PA over the 6 days at the end of each block. RESULTS: Meaningful latent differences between methods were observed for moderate PA and SB across all three assessment periods, such that participants underreported the time spent in each activity. Bland-Altman plots revealed a positive mean difference for vigorous PA, with over-reporting increasing as mean levels increased. Negative mean differences were observed for all other intensities. Underreporting of moderate PA increased as the mean level increased, whereas for light PA and SB, underreporting decreased at high levels. Repeated measures correlations revealed a meaningful association for vigorous PA only, suggesting that as self-reported minutes increase so too do device-measured minutes. CONCLUSIONS: We found evidence of cross-sectional and longitudinal differences and weak associations between self-reported and device-assessed PA and SB. Future work is needed to enhance the quality of self-reported methods to assess PA and SB (e.g., face and content validity), and consider improvements to the processing of device-based data.

Validity and reliability evidence of a point of care assessment of salivary cortisol and α-amylase: a pre-registered study.

PURPOSE: The iPro Cube is a small portable point-of-care device designed to analyse salivary markers of stress in a user-friendly manner (e.g., fast, convenient). Our aim was to test the reliability and validity of the iPro Cube to measure salivary cortisol and α-amylase as compared to the common laboratory standard method (ELISA immunoassay) prior to and after moderate intensity exercise. METHODS: The study was a repeated measures, pre-registered design, and statistical framework that incorporated prior knowledge directly into the estimation process. Twenty-nine individuals (age = 27.4 ± 6.6 y; body-mass = 70.8 ± 11.3 kg; height = 1.74 ± 0.92 m; 18 males) completed a single PWC75%HRmax, with repeated measures of salivary cortisol and -amylase pre, immediately post, and 30 min post-exercise. RESULTS: Correlation between the iPro Cube and laboratory-based assessments of salivary cortisol was moderate-to-large (0.53 > r < 0.81) across all three testing points. In contrast, correlation between the iPro Cube and laboratory-based assessments of -amylase was small-to-moderate (0.25 > r < 0.46). We found a large correlation between duplicate samples of iPro Cube cortisol assessment (0.75 > r < 0.82), and a moderate-to-large correlation for -amylase (0.51> r < 0.77). CONCLUSIONS: The iPro Cube is capable of taking measures of salivary cortisol that are moderately correlated to values obtained via ELISA immunoassay, however the unit underestimates salivary cortisol and overestimates salivary -amylase at rest and post-moderate intensity exercise. It is recommended that researchers continue using standard laboratory techniques to assess these salivary stress markers.

Profiles of adversity and resilience resources: A latent class analysis of two samples.

Adversities refer to events that are characterized by perceived or actual threat to human functioning. Often considered deleterious for health and well-being, recent work supports an alternative picture of the effects of adversity on human functioning, such that a moderate amount of adversity - when compared with none or high levels - can be beneficial. We extend this body of work in the current study by considering the breadth or type of adversities experienced simultaneously (referred to as polyadversity), with a focus on individual profiles of lifetime adversities. Latent class analysis was employed to explore different configurations of lifetime adversity experiences in two independent samples and examine how these latent classes differed with regard to resilience resources (i.e., optimism, hope, self-efficacy, and bounce-back ability). University students (N = 348) and members from the broader community (N = 1,506) completed measures of lifetime adversity exposure and resilience resources. Three polyadversity classes were revealed in each sample, with both producing a high and a low polyadversity class. The third class differed between samples; in the student sample, this class represented experiences of vicarious adversity, whereas in the community sample, it represented moderate levels of exposure to adversity. Support for the adaptive nature of a moderate amount of adversity exposure was found in the community sample but not in the student sample. This study produces initial evidence of how lifetime adversity experiences group together and how class membership is related to resilience resources.

Supplement Use and Behaviors of Athletes Affiliated With an Australian State-Based Sports Institute.

The authors aimed to update knowledge of the use of supplements among Australian athletes at a state-based sports institute. The authors conducted a cross-sectional survey using an online questionnaire to assess the influence of age, sports category, and scholarship category on supplement use. Of 94 completed questionnaires, 82 (87%) indicated supplements in the previous 12 months (mean = 4.9 ± 3.3). No significant difference in supplement usage rate was identified when considering age, scholarship category, or sport category. The most frequently used supplements were sports drinks (70%), caffeine (48%), protein powder (42%), and sports bars (42%). Recovery (63%), health maintenance (59%), and improved energy (50%) were the most frequently reported rationale to use supplements. Allied health professionals and credible online resources were the predominant sources of influence regarding use. However, athletes from lower scholarship categories were more likely to have social media, parents, and siblings influence usage, and age was inversely related to increased influence from parents, social media, physicians not associated with the institute, the Internet, and siblings. Older athletes and those on higher scholarships were more likely to source supplements from training facilities and sports nutrition staff outside of the institute or direct from a supplier, whereas those on lower scholarships tended to rely more on family and friends for their supplements. Findings from this study show a high prevalence of supplement use and are the first to show an influence of social media, particularly in younger athletes. Opportunities exist to optimize how athletes are informed regarding supplement use and organizational and supplement policy.

Mental toughness and behavioural perseverance: A conceptual replication and extension.

OBJECTIVES: The purpose of this study was to conduct a conceptual replication of the proposition that mental toughness is associated positively with behavioural perseverance. DESIGN: Repeated-measures design. METHODS: In total, 38 male Australian rules footballers took part in this study (age, 21±3 y; mass, 82.7±11.0kg; height, 1.84±.07m; football experience, 13±4 y). Participants self-reported mental toughness approximately one week prior to their first testing session where we assessed their aerobic capacity via the measurement of peak oxygen consumption (V˙O2peak). Approximately one week later, participants completed a 20m shuttle run test (MST). The final testing session took place approximately one week later, where participants completed a simulated team game circuit (STGC; 60min) to simulate game-relevant level of fatigue, which was followed immediately by a 20m MST. RESULTS: Mental toughness was a salient determinant of the variation in behavioural perseverance under typical circumstances, when prior knowledge from past research was incorporated directly into the estimation process. However, the positive association between mental toughness and behavioural perseverance did not generalise to a performance context in which participants were fatigued. CONCLUSIONS: The results of the current study suggest that mental toughness represents a salient psychological correlate of behavioural perseverance in a discrete physical task that taxes the aerobic energy system in some but not all situations. When fatigued, the effect of mental toughness is outweighed by greater underlying fitness.

When the going gets tough: Mental toughness and its relationship with behavioural perseverance.

OBJECTIVES: This study examined the association between self-reported mental toughness and behavioural perseverance among a sample of male Australian footballers in a naturalistic context. DESIGN: Cross-sectional field study, with the multistage 20m shuttle run test (MST) employed as a proxy for behavioural perseverance. METHODS: 330 male Australian footballers aged between 15 and 18 years (M=16.86; SD=.71) with between 2 and 14 years playing experience (M=9.32; SD=2.51) participated. Initially, footballers completed a mental toughness questionnaire, before having their height and body mass measurements taken. Subsequently, a performance testing session was completed, which included the 20m sprint, Australian football-specific agility run, vertical jump, and the MST. Bayesian estimation was employed to allow for the simultaneous examination of existing findings with our new data in a way that provides an automatic meta-analysis of evidence in this area. RESULTS: The analysis indicated a 95% probability that the association between mental toughness and behavioural perseverance lies between .14 and .34, even when controlling for other factors known to influence MST performance, including age, height, body mass, and years playing experience. CONCLUSIONS: Taken together with previous research, these findings support the theoretical proposition that persistence, effort or perseverance represents a behavioural signature of mental toughness.

Effect of beta-alanine supplementation on 800-m running performance.

UNLABELLED: Beta-alanine supplementation has been shown to improve exercise performance in short-term, high-intensity efforts. PURPOSE: The aim of this study was to assess if beta-alanine supplementation could improve 800 m track running performance in male recreational club runners (n = 18). METHODS: Participants completed duplicate trials (2 presupplementation, 2 postsupplementation) of an 800 m race, separated by 28 days of either beta-alanine (n = 9; 80 mg·kg-1BM·day-1) or placebo (n = 9) supplementation. RESULTS: Using ANCOVA (presupplementation times as covariate), postsupplementation race times were significantly faster following beta-alanine (p = .02), with post- versus presupplementation race times being faster after beta-alanine (-3.64 ± 2.70 s, -2.46 ± 1.80%) but not placebo (-0.59 ± 2.54 s, -0.37 ± 1.62%). These improvements were supported by a moderate effect size (d = 0.70) and a very likely (99%) benefit in the beta-alanine group after supplementation. Split times (ANCOVA) at 400 m were significantly faster (p = .02) postsupplementation in the beta-alanine group, compared with placebo. This was supported by large effect sizes (d = 1.05-1.19) and a very likely (99%) benefit at the 400 and 800 m splits when comparing pre- to postsupplementation with beta-alanine. In addition, the first and second halves of the race were faster post- compared with presupplementation following beta-alanine (1st half -1.22 ± 1.81 s, likely 78% chance of benefit; 2nd half -2.38 ± 2.31 s, d = 0.83, very likely 98% chance of benefit). No significant differences between groups or pre- and postsupplementation were observed for postrace blood lactate and pH. CONCLUSION: Overall, 28 days of beta-alanine supplementation (80 mg·kg-1BM·day-1) improved 800 m track performance in recreational club runners.

Effect of Beta alanine and sodium bicarbonate supplementation on repeated-sprint performance.

This study aimed to investigate if combining beta alanine (BA) and sodium bicarbonate (NaHCO3) supplementation could lead to enhanced repeated-sprint performance in team-sport athletes, beyond what is possible with either supplement alone. Participants (n = 24) completed duplicate trials of a repeated-sprint test (3 sets; 6 × 20 m departing every 25 seconds, 4 minutes active recovery between sets) and were then allocated into 4 groups as follows: BA only (n = 6; 28 days BA, acute sodium chloride placebo); NaHCO3 only (n = 6; 28 days glucose placebo, acute NaHCO3); BA/NaHCO3 (n = 6; 28 days BA, acute NaHCO3); placebo only (n = 6; 28 days glucose placebo, acute sodium chloride placebo), then completed duplicate trials postsupplementation. Sodium bicarbonate alone resulted in moderate effect size (d = 0.40-0.71) and "likely" and "very likely" benefit for overall total sprint times (TST) and for each individual set and for first sprint (sets 2 and 3) and best sprint time (sets 2 and 3). Combining BA and NaHCO3 resulted in "possible" to "likely" benefits for overall TST and for sets 2 and 3. First sprint (set 3) and best sprint time (sets 2 and 3) also showed "likely" benefit after this trial. The BA and placebo groups showed no differences in performance after supplementation. In conclusion, these results indicate that supplementation with acute NaHCO3 improved repeated-sprint performance more than either a combination of NaHCO3 and BA or BA alone.

Effect of beta-alanine supplementation on 2000-m rowing-ergometer performance.

UNLABELLED: Beta-alanine supplementation has been shown to improve exercise performance in short-term high-intensity efforts. However, whether supplementation with beta-alanine is ergogenic to actual sporting events remains unclear and should be investigated in field testing or race simulations. PURPOSE: The aim of this study was to assess if beta-alanine supplementation could improve 2,000-m rowing-ergometer performance in well-trained male rowers. METHODS: Participants (N = 16) completed duplicate trials (2 × before supplementation and 2 × after supplementation) of a 2,000-m rowing-ergometer race separated by 28 days of either beta-alanine (n = 7; 80 mg · kg-1 BM · d-1) or placebo (n = 9; glucose) supplementation. RESULTS: Beta-alanine group (pooled) race times improved by 2.9 ± 4.1 s and placebo group slowed by 1.2 ± 2.9 s, but these results were inconclusive for performance enhancement (p = .055, ES = 0.20, smallest worthwhile change = 49% beneficial). Race split times and average power outputs only significantly improved with beta-alanine at the 750-m (time -0.7 s, p = .01, power +3.6%, p = .03) and 1,000-m (time -0.5 s, p = .01, power +2.9%, p = .02) distances. Blood La- and pH postrace values were not different between groups before or after supplementation. CONCLUSIONS: Overall, 28 d of beta-alanine supplementation with 80 mg · kg-1 BM · d-1 (~7 g/d) did not conclusively improve 2,000-m rowing-ergometer performance in well-trained rowers.