Methane output across life stages in sheep, how it differs from lambs to adult ewes using portable accumulation chambers.

Methane (CH4) produced from enteric fermentation is a potent greenhouse gas produced by ruminant animals. Multiple measurements are required across life stages to develop an understanding of how CH4 output changes throughout the animal's lifetime. The objectives of the current study were to estimate CH4 output across life stages in sheep and to investigate the relationship between CH4 output and dry matter (DM) intake (DMI). Data were generated on a total of 266 female Suffolk and Texel animals. Methane and carbon dioxide (CO2) output, estimated using portable accumulation chambers, and DMI, estimated using the n-alkane technique outdoors and using individual penning indoors, were quantified across the animal's life stage; as lambs (<12 mo), nulliparous hoggets (12 to 24 mo) and ewes (primiparous or greater; > 24 mo). Ewes were further classified as pregnant, lactating, and dry (non-pregnant and non-lactating). Multiple measurements were taken within and across the life stages of the same animals. A linear mixed model was used to determine if CH4 and CO2 output differed across life stages and using a separate linear mixed model the factors associated with CH4 output within each life stage were also investigated. Methane, CO2 output, and DMI differed by life stage (P < 0.05), with lactating ewes producing the greatest amount of CH4 (25.99 g CH4/d) and CO2 (1711.6 g CO2/d), while also having the highest DMI (2.18 kg DM/d). Methane output differed by live-weight of the animals across all life stages (P < 0.001). As ewe body condition score increased CH4 output declined (P < 0.05). Correlations between CH4 output measured across life stages ranged from 0.26 (SE 0.08; lambs and lactating ewes) to 0.59 (SE 0.06; hoggets and pregnant ewes), while correlations between CO2 output measured across life stages ranged from 0.12 (SE 0.06; lambs and hoggets) to 0.65 (SE 0.06; hoggets and lactating ewes). DMI was moderately correlated with CH4 (0.44; SE 0.04) and CO2 output (0.59; SE 0.03). Results from this study provide estimates of CH4 output across life stages in a pasture-based sheep production system and offer valuable information for the national inventory and the marginal abatement cost curve on the optimum time to target mitigation strategies.

Obtaining accurate estimates of methane (CH4) output across life stages is important to assess how CH4 output changes throughout the production cycle in pasture-based sheep production systems. This study investigated the factors associated with CH4 output at each life stage (lambs, hoggets, pregnant, lactating, and dry ewes), the relationship between CH4 output measured across life stages and the relationship between CH4 output and dry matter intake (DMI) in an Irish lowland sheep production system. Methane and carbon dioxide (CO2) output and DMI were measured on 266 purebred Suffolk and Texel females across their lifetime. Lactating ewes produced the highest CH4 and CO2 output, along with having the highest DMI. Across all life stages, CH4 output increased with increasing live weight while CH4 output decreased as body condition score increased. Weak to moderate relationships were found between CH4 output measured across life stages, with the strength of the relationship decreasing as the time between life stages increased. A positive relationship was found between DMI and CH4 output. Results from this study lead to the development of a profile of CH4 output across the production cycle of a pasture-based sheep system.

Repeatability of gaseous measurements across consecutive days in sheep using portable accumulation chambers.

Portable accumulation chambers (PACs) enable gaseous emissions from small ruminants to be measured over a 50-min period; to date, however, the repeatability of consecutive days of measurement in the PAC has not been investigated. The objectives of this study were 1) to investigate the repeatability of consecutive days of gaseous measurements in the PAC, 2) to determine the number of days required to achieve precise gaseous measurements, and 3) to develop a prediction equation for gaseous emissions in sheep. A total of 48 ewe lambs (c. 10 to 11 mo of age) were randomly divided into four measurement groups each day, for 17 consecutive days. Gaseous measurements were conducted between 0800 and 1200 hours daily. Animals were removed from perennial ryegrass silage for at least 1 h before measurements in the PAC, and animals were assigned randomly to each of the 12 chambers. Methane (CH4; ppm) concentration, oxygen (O2; %), and carbon dioxide (CO2; %) were measured at three time points (0, 25, and 50 min after entry of the first animal into the first chamber). To quantify the effect of animal and day variation on gaseous emissions, between-animal, between-day, and error variances were calculated for each gaseous measurement using a linear mixed model. The number of days required to gain a certain precision (defined as the 95% confidence interval range) for each gaseous measurement was also calculated. For all three gases, the between-day variance (39% to 40%) accounted for a larger proportion of total variance compared with between-animal variance, while the repeatability of 17 consecutive days of measurement was 0.36, 0.31, and 0.23 for CH4, CO2, and O2, respectively. Correlations between consecutive days of measurement were strong for all three gases; the strongest correlation between day 1 and the remaining days for CH4, CO2, and O2 was 0.71 (days 1 and 6), 0.77 (days 1 and 2), and 0.83 (days 1 and 5), respectively. A high level of precision was achieved when gaseous measurements from PAC were taken over three consecutive days. The prediction equation overestimated gaseous production for all three gases: the correlations between actual and predicted gaseous output ranged from 0.67 to 0.71, with the r2 ranging from 0.45 to 0.71. The results from this study will aid the refinement of the protocol for the measurement of gaseous emissions in sheep using the PAC.

Investigation of intra-day variability of gaseous measurements in sheep using portable accumulation chambers.

Portable accumulation chambers (PAC) enable short-term spot measurements of gaseous emissions including methane (CH4), carbon dioxide (CO2), and oxygen (O2) consumption from small ruminants. To date the differences in morning and evening gaseous measurements in the PAC have not been investigated. The objectives of this study were to investigate: 1) the optimal measurement time in the PAC, 2) the appropriate method of accounting for the animal's size when calculating the animal's gaseous output, and 3) the intra-day variability of gaseous measurements. A total of 12 ewe lambs (c. 10 to 11 months of age) were randomly selected each day from a cohort of 48 animals over nine consecutive days. Methane emissions from the 12 lambs were measured in 12 PAC during two measurement runs daily, AM (8 to 10 h) and PM (14 to 16 h). Animals were removed from Perennial ryegrass silage for at least 1 h prior to measurements in the PAC and animals were assigned randomly to each of the 12 chambers. Methane (ppm) concentration, O2 and CO2 percentage were measured at 5 time points (T1 = 0.0 min, T2 = 12.5 min, T3 = 25.0 min, T4 = 37.5 min, and T5 = 50.0 min from entry of the first animal into the first chamber) using an Eagle 2 monitor. The correlation between time points T5-T1 (i.e., 50 min minus 0 min after entry of the animal to the chamber) and T4-T1 was 0.95, 0.92, and 0.77 for CH4, O2, and CO2, respectively (P < 0.01). The correlation between CH4 and CO2 output and O2 consumption, calculated with live-weight and with body volume was 0.99 (P < 0.001). The correlation between the PAC measurement recorded on the same animal in the AM and PM measurement runs was 0.73. Factors associated with CH4 production included: day and time of measurement, the live-weight of the animal and the hourly relative humidity. Results from this study suggest that the optimal time for measuring an animal's gaseous output in the PAC is 50 min, that live-weight should be used in the calculation of gaseous output from an animal and that the measurement of an animal's gaseous emissions in either the AM or PM does not impact on the ranking of animals when gaseous emissions are measured using the feeding and measurement protocol outlined in the present study.