Nursing education strategy for caregivers of children and adolescents with epilepsy.

Epilepsy is a disease that commonly manifests in childhood and can significantly affect the quality of life for both sufferers and their families. As such, it necessitates comprehensive and high-quality healthcare. OBJECTIVE: To assess the impact of an educational intervention targeted to caregivers of children and adolescents with epilepsy. MATERIALS AND METHODS: A cross-sectional study conducted between February and November 2022, involving 140 patients and their primary caregivers who intentionally sought medical consultation at a pediatric hospital in Caldas, Colombia. Initial nursing consultations were conducted to identify clinical and demographic characteristics and to assess knowledge about the disease, its management, and care. Subsequently, a comprehensive educational process was implemented using various educational aids. After 60 days, feedback was provided to evaluate the caregivers' learning. RESULTS: Focal epilepsy, generalized epilepsy and idiopathic focal epilepsy predominated in the medical diagnoses. The etiology highlighted genetic cause and structural 70% of caregivers were female. Caregivers' schooling: 10% of caregivers did not have schooling, 28% had primary education, 46% had secondary education, 16% had postgraduate education. Caregivers demonstrated knowledge about epilepsy and its management, with the majority scoring above 50%. The posttest indicated improvement in learning processes, and caregivers expressed that it benefited them in learning first aid. CONCLUSION: Establishing educational programs tailored to the needs of individuals and promoting care plans are essential for improving the living conditions of patients with epilepsy and their caregivers.

Alternative splicing as a driver of natural variation in abscisic acid response.

Abscisic acid (ABA) is a crucial player in plant responses to the environment. It accumulates under stress, activating downstream signaling to implement molecular responses that restore homeostasis. Natural variance in ABA sensitivity remains barely understood, and the ABA pathway has been mainly studied at the transcriptional level, despite evidence that posttranscriptional regulation, namely, via alternative splicing, contributes to plant stress tolerance. Here, we identified the Arabidopsis accession Kn-0 as less sensitive to ABA than the reference Col-0, as shown by reduced effects of the hormone on seedling establishment, root branching, and stomatal closure, as well as by decreased induction of ABA marker genes. An in-depth comparative transcriptome analysis of the ABA response in the two variants revealed lower expression changes and fewer genes affected for the least ABA-sensitive ecotype. Notably, Kn-0 exhibited reduced levels of the ABA-signaling SnRK2 protein kinases and lower basal expression of ABA-reactivation genes, consistent with our finding that Kn-0 contains less endogenous ABA than Col-0. ABA also markedly affected alternative splicing, primarily intron retention, with Kn-0 being less responsive regarding both the number and magnitude of alternative splicing events, particularly exon skipping. We find that alternative splicing introduces a more ecotype-specific layer of ABA regulation and identify ABA-responsive splicing changes in key ABA pathway regulators that provide a functional and mechanistic link to the differential sensitivity of the two ecotypes. Our results offer new insight into the natural variation of ABA responses and corroborate a key role for alternative splicing in implementing ABA-mediated stress responses.

Marie Poussepin's Influence on Nursing from Her Vocation of Service and Charity / Influencia de Marie Poussepin a la enfermería desde su vocación de servicio y caridad / Influência de Marie Poussepin na enfermagem a partir de sua vocação de serviço e caridade

Objective. This work sought to describe the influence of Marie Poussepin on Nursing from her vocation of service and charity. Method. Historical-hermeneutic study with participation by 15 Dominican Sisters of Charity in the Presentation of the Blessed Virgin from the city of Manizales and Bogotá, Colombia, who answered semi-structured interviews. The information was gathered and recontextualized via the open and axial coding system through ATLAS.ti9 software. During the interpretation procedure, copying, intensive reading, note taking, analysis, first epigraph of the report, coding, grouping, and determination of categories was made, conducting information triangulation with existing evidence. Results.Three categories emerged: Responding to the call of Jesus through service to the community; Under the legacy of charity, respect for life and the dignity of human beings, and Caring for life as a foundation of nursing. The second category formulated the description that integrates the course of life and objectives of the institutions where the nuns interviewed work. Conclusion.The legacy byMarie Poussepin to nursing care has been manifested since the foundation of the work, influencing the disciplinary work through the vocational commitment of those who make up the congregation, imprinting a character of service and respect for others, in response to the love of God.

Objetivo. Describir la influencia de Marie Poussepin para la enfermería, desde su vocación de servicio y caridad. Método. Estudio histórico-hermenéutico. Participaron quince Hermanas de la Caridad Dominicas de la Presentación de la Santísima Virgen de la ciudad de Manizales y Bogotá, Colombia, quienes respondieron a entrevistas semiestructuradas. Se recogió y recontextualizó la información mediante el sistema de codificación abierta y axial del software ATLAS.ti9. Durante el procedimiento de interpretación se hizo copia, lectura intensiva, toma de notas, análisis, primer epígrafe del informe, codificación, agrupación y determinación de categorías, y se llevó a cabo la triangulación de la información con la evidencia existente. Resultados. Emergieron tres categorías: Dando respuesta al llamado de Jesús mediante el servicio a la comunidad; Bajo el legado de caridad, respeto por la vida y la dignidad del ser humano y Cuidado de la vida como fundamento de enfermería. En la segunda categoría se realizó la descripción que integra el curso de vida y objetivo de las instituciones donde laboran las religiosas entrevistadas. Conclusión.El legado de Marie Poussepin, al cuidado enfermero, se ha manifestado desde la fundación de la obra y ha influido en la labor disciplinar mediante el compromiso vocacional de quienes integran la congregación, e imprime un carácter de servicio y respeto por el otro en respuesta al amor a Dios.

Objetivo. Descrever a influência de Marie Poussepin para a enfermagem, a partir de sua vocação de serviço e caridade. Método. Estudo histórico-hermenêutico. Participaram quinze irmãs da Caridade Dominicanas da Apresentação da Santíssima Virgem da cidade de Manizales e Bogotá, Colômbia, respondendo a entrevistas semiestruturadas. As informações foram coletadas e recontextualizadas por meio do sistema de codificação aberta e axial por meio do software ATLAS.ti9. Durante o procedimento de interpretação, foram feitas cópia, leitura intensiva, anotações, análise, primeira epígrafe do relato, codificação, agrupamento e determinação de categorias, realizando-se a triangulação das informações com as evidências existentes. Resultados. Emergiram três categorias: Respondendo ao chamado de Jesus através do serviço à comunidade; Sob o legado da caridade, o respeito à vida e à dignidade da pessoa humana e o cuidado à vida como fundamento da enfermagem. Na segunda categoria, foi feita a descrição que integra o percurso de vida e objetivo das instituições onde trabalham as freiras entrevistadas. Conclusão. O legado de Marie Poussepin para o cuidado de enfermagem se manifesta desde a fundação da obra, influenciando o trabalho disciplinar através do compromisso vocacional daqueles que compõem a congregação, imprimindo um caráter de serviço e respeito ao próximo, em resposta ao amor a Deus.

Effects of Socio-educational Interventions on the Quality of Life of People with a Digestive Ostomy.

Introduction: The creation of digestive stomata is associated with a change in people's lives. Causing a strong impact that influences all dimensions of life. This paper reports on the effects of socio-educational interventions on the quality of life (QOL) of people with a digestive ostomy. Objective: To describe the effect of a nursing intervention on the QOL of people with a digestive ostomy. Methods: A quasiexperimental study was conducted whose sample consisted of 12 people who were ostomized in a public hospital in Colombia. Participants were selected through convenience sampling and randomly assigned to the intervention group and the control group. For the control group, an educational process was carried out through a theoretical session with virtual mediation (educational video). The QOL was evaluated before and after the interventions through the Montreux questionnaire. Results: The average age was 57(+7) years. No statistical differences were found between the groups in the QOL index or for any of the dimensions that make up QOL. Pretest and post-test analysis for each intervention separately showed improvement in two dimensions of QOL for each group; in the intervention group body image dimension (p = .017) and the positive coping dimension (p = .027). In the control group, the physical well-being dimension (p = .037) and social concerns dimension (p = .034). Conclusions: The personalized educational intervention or carried out through virtual pedagogical mediation, generated a clinically significant increase in the dimensions of QOL, without statistical differences. The study adds knowledge about the impact that digestive stomata have on the QOL, which is why it is necessary to establish specialized interdisciplinary teams to care for the person's new condition at home during the following months.

The SCL30a SR protein regulates ABA-dependent seed traits and germination under stress.

SR proteins are conserved RNA-binding proteins best known as splicing regulators that have also been implicated in other steps of gene expression. Despite mounting evidence for a role in plant development and stress responses, the molecular pathways underlying SR protein regulation of these processes remain poorly understood. Here we show that the plant-specific SCL30a SR protein negatively regulates ABA signaling to control seed traits and stress responses during germination in Arabidopsis. Transcriptome-wide analyses revealed that loss of SCL30a function barely affects splicing, but largely induces ABA-responsive gene expression and genes repressed during germination. Accordingly, scl30a mutant seeds display delayed germination and hypersensitivity to ABA and high salinity, while transgenic plants overexpressing SCL30a exhibit reduced ABA and salt stress sensitivity. An ABA biosynthesis inhibitor rescues the enhanced mutant seed stress sensitivity, and epistatic analyses confirm that this hypersensitivity requires a functional ABA pathway. Finally, seed ABA levels are unchanged by altered SCL30a expression, indicating that the gene promotes seed germination under stress by reducing sensitivity to the phytohormone. Our results reveal a new player in ABA-mediated control of early development and stress response.

ABA signaling prevents phosphodegradation of the SR45 splicing factor to alleviate inhibition of early seedling development in Arabidopsis.

Serine/arginine-rich (SR) proteins are conserved splicing regulators that play important roles in plant stress responses, namely those mediated by the abscisic acid (ABA) hormone. The Arabidopsis thaliana SR-like protein SR45 is a described negative regulator of the ABA pathway during early seedling development. How the inhibition of growth by ABA signaling is counteracted to maintain plant development under stress conditions remains largely unknown. Here, we show that SR45 overexpression reduces Arabidopsis sensitivity to ABA during early seedling development. Biochemical and confocal microscopy analyses of transgenic plants expressing fluorescently tagged SR45 revealed that exposure to ABA dephosphorylates the protein at multiple amino acid residues and leads to its accumulation, due to SR45 stabilization via reduced ubiquitination and proteasomal degradation. Using phosphomutant and phosphomimetic transgenic Arabidopsis lines, we demonstrate the functional relevance of ABA-mediated dephosphorylation of a single SR45 residue, T264, in antagonizing SR45 ubiquitination and degradation to promote its function as a repressor of seedling ABA sensitivity. Our results reveal a mechanism that negatively autoregulates ABA signaling and allows early plant growth under stress via posttranslational control of the SR45 splicing factor.

Marie Poussepin's Influence on Nursing from Her Vocation of Service and Charity.

Objective: This work sought to describe the influence of Marie Poussepin on Nursing from her vocation of service and charity. Methods: Historical-hermeneutic study with participation by 15 Dominican Sisters of Charity in the Presentation of the Blessed Virgin from the city of Manizales and Bogotá, Colombia, who answered semi-structured interviews. The information was gathered and recontextualized via the open and axial coding system through ATLAS.ti9 software. During the interpretation procedure, copying, intensive reading, note taking, analysis, first epigraph of the report, coding, grouping, and determination of categories was made, conducting information triangulation with existing evidence. Results: Three categories emerged: Responding to the call of Jesus through service to the community; Under the legacy of charity, respect for life and the dignity of human beings, and Caring for life as a foundation of nursing. The second category formulated the description that integrates the course of life and objectives of the institutions where the nuns interviewed work. Conclusion: The legacy by Marie Poussepin to nursing care has been manifested since the foundation of the work, influencing the disciplinary work through the vocational commitment of those who make up the congregation, imprinting a character of service and respect for others, in response to the love of God.

Síntomas espirituales de adultos mayores en tiempos de pandemia por COVID-19 / Spiritual symptoms of aged in times of a COVID-19 pandemic

Resumen: Objetivo: Describir y correlacionar los síntomas espirituales que presentan los adultos mayores del municipio de Caldas, Colombia en tiempos de pandemia COVID-19. Materiales y Método: Estudio cuantitativo, descriptivo, observacional y correlacional, con 300 personas mayores, se aplicó un instrumento validado sobre síntomas espirituales, analizando con estadística descriptiva y utilizando la prueba de Pearson. Resultados: Los síntomas de espiritualidad se evidenciaron moderados en cuanto al dolor espiritual con 34%, autocastigo 35%, búsqueda espiritual proyectada se detectó que en un 36% estaban en severo, al igual que angustia espiritual 42%, incredulidad 45%, desesperanza y desamor 48%, se encontró significancia estadística correlacionando signos espirituales con edad, sexo, ocupación, estado civil, enfermedad, presencia de hijos y necesidad de ayuda para las actividades diarias. Conclusiones: Se concluye que los resultados permiten describir los síntomas espirituales, así como hacer correlaciones entre los síntomas y las diversas características de los participantes. Más aún, estos resultados permiten reflexionar que el cuidado a los adultos mayores, debe considerar además de la cuestión biológica, la situación espiritual, demostrando que hay que cuidarlas de manera integral para mejorar el estado de salud y la calidad de vida de los adultos mayores, por lo que se deben considerar los problemas del proceso de envejecimiento en sus distintos contextos, proponer modelos multidisciplinares y políticas de salud que mejoren la calidad y condiciones de vida de este sector de la población y de sus cuidadores.

Abstract: Objective: To describe and to correlate the spiritual symptoms presented by the elderly in the municipality of Caldas, Colombia in times of the COVID-19 pandemic. Materials and Method: Quantitative, descriptive, observational study, with 300 elderly people, a validated instrument on spiritual symptoms was applied, with descriptive statistics using Pearson test Results: spirituality symptoms were moderate in terms of spiritual pain with 34%, self-punishment 35%, projected spiritual search was found to be severe in 36% as well as spiritual anguish 42%, disbelief 45%, hopelessness and heartbreak 48%, statistical significance was found correlating spiritual signs with age, sex, occupation, marital status, illness, presence of children and need for help with daily activities. Conclusions: It is concluded that the results allow describing the spiritual symptoms, as well as making correlations between the symptoms and the various characteristics of the participants. Furthermore, these results allow us to reflect that the care of the elderly must consider, in addition to the biological dimension, the spiritual dimension, showing that care must be taken in a holistic way to improve the health status and quality of life of the elderly Therefore, the problems of the aging process should be considered in its different contexts, and multidisciplinary models and health policies should be proposed that improve the quality and living conditions of this sector of the population and its caregivers.

Etiolated Hypocotyls: A New System to Study the Impact of Abiotic Stress on Cell Expansion.

Abiotic stress impacts a wide range of plant developmental processes. Among them, cell expansion is particularly important given its contribution to plant growth and morphogenesis. Here, we describe a new phenotypic system to quantify accurately the impact of different sources of abiotic stress on the cell's capacity to expand. This approach monitors hypocotyl growth in Arabidopsis thaliana etiolated seedlings, as in the dark this embryonic organ is known to grow solely by expanding its cells, without cell division.

Assessing Abscisic Acid-Mediated Changes in Stomatal Aperture Through High-Quality Leaf Impressions.

Plants live in highly dynamic surroundings and need to cope with constant environmental challenges. In order to do so, they developed quick reactions to stress that allow them to gain time while mounting a major response. This first line of defense includes the stomata, leaf epidermal pores in charge of regulating water loss and photosynthesis. Stomatal movements are controlled by the stress phytohormone abscisic acid (ABA), which induces fast closure of the stomata upon perception of stress conditions. By modulating plasma membrane ion channels, ABA leads to loss of water from the guard cells surrounding the stomatal pore and a consequent reduction of its aperture. Here, we provide a microscopy-based method to assess the plant's response to ABA through measurements of the stomatal aperture. This protocol describes a simple, quick, and unexpensive method to prepare high-quality impressions of leaves from Arabidopsis thaliana seedlings from long-lasting silicone-based casts, allowing detailed imaging and accurate determination of the aperture of stomatal pores.