Molecular detection of tick-borne pathogens in captive wild felids, Zimbabwe.

BACKGROUND: The populations of wild felids in Africa, of especially lions (Panthera leo) and cheetahs (Acinonyx jubatus), are declining and the species are classified as vulnerable to extinction by the International Union for Conservation of Nature. As infections with tick-borne pathogens (TBP) can become more of a problem in wild felids, there are relatively few studies on TBP in wild felids in Africa and on how these infections might influence population numbers. METHODS: To gain further knowledge on TBP in captive wild felids in Southern Africa, we collected whole blood from captive lions, Southern African wildcats, cheetahs and servals in Zimbabwe for PCRs against the 18S rRNA gene of the piroplasmids (Babesia, Theileria, Cytauxzoon) and Hepatozoon spp., and the 16S rRNA gene of Ehrlichia and Anaplasma spp. RESULTS: Overall, 78% of the lions (67/86) and all the Southern African wildcats (6/6), cheetahs (4/4) and servals (2/2) had evidence of infection with at least one organism. The organisms most commonly detected in the lions were B. leo (59%; 51/86), B. vogeli (12%; 10/86) and H. felis (11%; 9/86) while all the Southern African wildcats and servals were positive for B. vogeli and all the cheetahs were positive for B. leo. Mixed infections were found in 22% (15/67) of the PCR positive lions, most commonly B. leo and H. felis (27%; 4/15), and in 1 (50%) of the servals (B. vogeli and A. phagocytophilum). Two lions were infected with three TBP, mainly B. leo, H. canis and T. parva, and B. leo, A. phagocytophilum and T. sinensis. Mixed infections with B. vogeli and A. phagocytophilum were seen in a serval and a Southern African wildcat. Other TBP were detected at a low prevalence (≤2%) in lions, mainly H. canis, T. sinensis, T. parva, C. manul, E. canis, and E. canis-like and B. odocoilei-like organisms. CONCLUSIONS: Infections with tick-borne agents are common in captive wild felids in Zimbabwe.

Serological survey of Brucella canis in dogs in urban Harare and selected rural communities in Zimbabwe.

A cross-sectional study was conducted in order to detect antibodies for Brucella canis (B. canis) in dogs from urban Harare and five selected rural communities in Zimbabwe. Sera from randomly selected dogs were tested for antibodies to B. canis using an enzyme-linked immunosorbent assay. Overall, 17.6% of sera samples tested (57/324, 95% CI: 13.5-21.7) were positive for B. canis antibodies. For rural dogs, seroprevalence varied from 11.7% - 37.9%. Rural dogs recorded a higher seroprevalence (20.7%, 95% CI: 15.0-26.4) compared with Harare urban dogs (12.7%, 95% CI: 6.9-18.5) but the difference was not significant (p = 0.07). Female dogs from both sectors had a higher seroprevalence compared with males, but the differences were not significant (p > 0.05). Five and two of the positive rural dogs had titres of 1:800 and 1:1600, respectively, whilst none of the positive urban dogs had a titre above 1:400. This study showed that brucellosis was present and could be considered a risk to dogs from the studied areas. Further studies are recommended in order to give insight into the epidemiology of brucellosis in dogs and its possible zoonotic consequences in Zimbabwe. Screening for other Brucella spp. (Brucella abortus, Brucella melitensis and Brucella suis) other than B. canis is also recommended.

Seroprevalence of leptospirosis in dogs in urban Harare and selected rural communities in Zimbabwe.

A cross-sectional study was conducted to investigate seroprevalence of canine leptospirosis in urban Harare and five selected rural communities in Zimbabwe and to assess public awareness of the disease. Sera from randomly selected dogs were tested for antibodies to the serovars Canicola, Grippotyphosa, Icterohaemorrhagiae and Pomona of Leptospira interrogans using an enzyme-linked immunosorbent assay. Clinical chemistry was performed on all seropositive and selected seronegative sera to screen for hepatic and renal insufficiency. A questionnaire- based survey was conducted in Harare to assess dog owners' awareness of leptospirosis and other zoonoses. Overall, 15.6% of sera samples tested (39 out of 250; 95% confidence interval [CI]: 11.0% - 20.2%) were positive for leptospiral antibodies. A significantly higher (p < 0.05) seroprevalence was recorded in urban dogs than in rural dogs (25% vs. 11.2%). No significant difference in seroprevalence was observed amongst dogs from different rural communities or between sexes of dogs. There was a significant association between seropositivity and hepatic and/or renal insufficiency (p < 0.01), with dogs having hepatic and/or renal insufficiency being approximately twice as likely to be seropositive (relative risk = 1.96; 95% CI: 1.3-3.0). Of the dog owners, 78.8% (119/151) were aware of zoonoses. Except for rabies (92.4%), awareness of leptospirosis (5.0%) and other zoonoses amongst these owners was low. This study showed that leptospirosis was present and represented a risk to dogs from urban Harare and the selected rural communities in Zimbabwe. Availing training programmes for dog owners would be beneficial in improving disease control and reducing the public health risk of pet zoonoses.