RESUMO
Macrophages are key contributors to vascularization, but the mechanisms behind their actions are not understood. Here, we show that diverse macrophage phenotypes have distinct effects on endothelial cell behavior, with resulting effects on vascularization of engineered tissues. In Transwell coculture, proinflammatory M1 macrophages caused endothelial cells to up-regulate genes associated with sprouting angiogenesis, whereas prohealing (M2a), proremodeling (M2c), and anti-inflammatory (M2f) macrophages promoted up-regulation of genes associated with pericyte cell differentiation. In 3D tissue-engineered human blood vessel networks in vitro, short-term exposure (1 day) to M1 macrophages increased vessel formation, while long-term exposure (3 days) caused regression. When human tissue-engineered blood vessel networks were implanted into athymic mice, macrophages expressing markers of both M1 and M2 phenotypes wrapped around and bridged adjacent vessels and formed vessel-like structures themselves. Last, depletion of host macrophages inhibited remodeling of engineered vessels, infiltration of host vessels, and anastomosis with host vessels.
Assuntos
Células Endoteliais , Engenharia Tecidual , Animais , Diferenciação Celular , Macrófagos , Camundongos , FenótipoRESUMO
Metastatic papillary adenocarcinomas of the ovary are rare compared to primary ovarian papillary serous carcinomas. We report a case of pulmonary papillary adenocarcinoma metastatic to the ovary and show how this tumor can be differentiated immunohistochemically from an ovarian primary. Paraffin blocks of the ovarian tumor were analyzed for carcinoembryonic antigen, CA 125, surfactant, E-cadherin, N-cadherin, and vimentin. These markers are useful in differentiating epithelial tumors of lung versus ovarian origin. The papillary tumor showed expression of carcinoembryonic antigen, surfactant, and E-cadherin, but was negative for CA 125, N-cadherin, and vimentin. These findings support a lung carcinoma metastatic to the ovary.
Assuntos
Adenocarcinoma Papilar/secundário , Neoplasias Pulmonares/patologia , Neoplasias Ovarianas/secundário , Adenocarcinoma Papilar/química , Adenocarcinoma Papilar/tratamento farmacológico , Adulto , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Biomarcadores Tumorais/análise , Antígeno Ca-125/análise , Caderinas/análise , Antígeno Carcinoembrionário/análise , Feminino , Humanos , Imuno-Histoquímica , Neoplasias Pulmonares/química , Neoplasias Pulmonares/tratamento farmacológico , Proteínas de Neoplasias/análise , Neoplasias Ovarianas/química , Neoplasias Ovarianas/cirurgia , Surfactantes Pulmonares/análise , Vimentina/análiseAssuntos
Alanina/análise , Proteínas/análise , Ácidos Sulfínicos/análise , Animais , Oxirredução , LãRESUMO
Dans la partie continentale des États-Unis d'Amérique il y a trois foyers de lèpre: l'un, sur la Côte du Pacifique ou la majorité des maladie est fournie par la population asiatique; le deuxième dans le Minnesota, dans la région des Grands Lacs ou les personnes attaqueés sont de la race scandinave, et le troisième le long de la côte du Golfe du Mexique, spécialement en Louisiane, en Floride et au Texas