Effect of different quality of light on growth and production of secondary metabolites in adventitious root cultivation of Hypericum perforatum.

Naphthodianthrone derivatives that produced in Hypericum perforatum (St. John's Wort) are valuable secondary metabolites for depression treatment and photodynamic therapy. However, the traditional cultivation of this plant does not meet both quantitatively and qualitatively the high demand of the pharmaceutical industry. So, the adventitious root culture along with elicitation has been introduced as an alternative for production of such valuable bioactive compounds. The aim of this study was to evaluate the effect of darkness and red, blue and fluorescent light on growth and production of secondary metabolites in the adventitious root cultivation of H. perforatum. Our results showed that biomass production was significantly higher in the cultures grown under dark and red light, but in terms of hypericins production, red light was the best. Despite the inhibitory effect of five weeks blue light treatment on both biomass and secondary metabolite production of adventitious roots, one-week blue light treatment of four-weeks grown roots is an effective stimulator for increasing total phenolic compounds and hypericins. Interestingly, the roots were regenerated under red light and stems and leaves were formed.

Bioreactor-based advances in plant tissue and cell culture: challenges and prospects.

Bioreactors are engineered systems capable of supporting a biologically active situation for conducting aerobic or anaerobic biochemical processes. Stability, operational ease, improved nutrient uptake capacity, time- and cost-effectiveness, and large quantities of biomass production, make bioreactors suitable alternatives to conventional plant tissue and cell culture (PTCC) methods. Bioreactors are employed in a wide range of plant research, and have evolved over time. Such technological progress, has led to remarkable achievements in the field of PTCC. Since the classification of bioreactors has been extensively reviewed in numerous reviews, the current article avoids repeating the same material. Alternatively, it aims to highlight the principal advances in the bioreactor hardware s used in PTCC rather than classical categorization. Furthermore, our review summarizes the most significant steps as well as current state-of-the-art of PTCC carried out in various types of bioreactor.

Oil palm EgCBF3 conferred stress tolerance in transgenic tomato plants through modulation of the ethylene signaling pathway.

CBF/DREB1 is a group of transcription factors that are mainly involved in abiotic stress tolerance in plants. They belong to the AP2/ERF superfamily of plant-specific transcription factors. A gene encoding a new member of this group was isolated from ripening oil palm fruit and designated as EgCBF3. The oil palm fruit demonstrates the characteristics of a climacteric fruit like tomato, in which ethylene has a major impact on the ripening process. A transgenic approach was used for functional characterization of the EgCBF3, using tomato as the model plant. The effects of ectopic expression of EgCBF3 were analyzed based on expression profiling of the ethylene biosynthesis-related genes, anti-freeze proteins (AFPs), abiotic stress tolerance and plant growth and development. The EgCBF3 tomatoes demonstrated altered phenotypes compared to the wild type tomatoes. Delayed leaf senescence and flowering, increased chlorophyll content and abnormal flowering were the consequences of overexpression of EgCBF3 in the transgenic tomatoes. The EgCBF3 tomatoes demonstrated enhanced abiotic stress tolerance under in vitro conditions. Further, transcript levels of ethylene biosynthesis-related genes, including three SlACSs and two SlACOs, were altered in the transgenic plants' leaves and roots compared to that in the wild type tomato plant. Among the eight AFPs studied in the wounded leaves of the EgCBF3 tomato plants, transcript levels of SlOSM-L, SlNP24, SlPR5L and SlTSRF1 decreased, while expression of the other four, SlCHI3, SlPR1, SlPR-P2 and SlLAP2, were up-regulated. These findings indicate the possible functions of EgCBF3 in plant growth and development as a regulator of ethylene biosynthesis-related and AFP genes, and as a stimulator of abiotic stress tolerance.