RESUMO
We have determined the concentration of Lp(a) in an Ivory Coast population (n=102) using two immunochemical methods: Laurell's monodimensional electro-immunodiffusion (EID) and immunonephelometry (IN). Within-run and between-run precision was respectively 3.07% and 3.97% by IN and 1.52% and 4.48% by EID method. As regard the exactitude, the bias goals in two methods were 3.5% and 3.0% respectively with IN and EID. The two methods were correlated (r=0.84; p=0.006). Mean values of Lp(a) were significantly (p=0.0007) higher by IN than EID: 0.48+/-0.34 g/L versus 0.32+/-0.19 g/L. The Lp(a) distributions were non-Gaussian, skewed towards high values, with median value of 0.47 g/L and 0.32 g/L respectively for IN and EID methods. Therefore, we conclude that although both methods showed a satisfactory precision, and results were correlated, Lp(a) values were higher by INP. Furthermore, mean values of Lp(a) in presumed healthy Ivorian is higher than in Caucasians.
Assuntos
Imunoensaio/métodos , Lipoproteína(a)/sangue , Adolescente , Adulto , Côte d'Ivoire , Humanos , Imunodifusão , Pessoa de Meia-Idade , Nefelometria e Turbidimetria , Valores de Referência , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
The purpose of this case-control study was to determine the variations of lipoproteins during stroke in adults from Ivory Coast. The survey included 72 subjects presenting with hemorrhagic stroke and 58 of ischemic stroke, aged of 25 at 93 years. Lipids parameters have been measured by a colorimetric enzymatic method. Apolipoproteins have been determined by immunoturbidimetry. Results showed significant decrease of HDL-cholesterol and apoprotein A1 in patients by comparison with control subjects. More over, an increase of the atherogenicity index expressed as total/HDL-cholesterol or apolipoprotein B /apolipoprotein A1, of the triglycerides and apolipoprotein B has been observed.