Non-invasive assessment of soluble skin surface biomarkers in atopic dermatitis patients-Effect of treatment.

BACKGROUND: Skin biomarkers are important tools for characterizing specific disease processes in atopic dermatitis (AD) patients and can be used for monitoring and potentially predicting treatment response. Recent developments of minimally invasive skin sampling methods have made sampling easier and less inconvenient for patients. The objective of this study was to evaluate the non-invasive patch technique developed by FibroTx for skin biomarker analysis. MATERIALS AND METHODS: Ten adult patients with AD were included in the study and treated with topical corticosteroid (diprosone 0.05%) for 2 weeks. Skin surface biomarkers were assessed in three lesional and non-lesional sites before and during treatment using the FibroTx Patch method. Skin tape strips were also collected from the subjects for comparison. RESULTS: The results showed expression of IL-1 cytokine family members, chemokines, and defensins on lesional and non-lesional skin. Several of these markers were strongly reduced by topical treatment. The biomarker expression in skin surface eluates correlated strongly with those seen in skin tape strips from the same subjects. CONCLUSION: These data further support the usefulness of non-invasive sampling methods for assessing inflammatory processes in AD skin and demonstrate that the patch sampling method is a good alternative to skin tape strips.

Allergic contact dermatitis is accompanied by severe abnormal changes in antioxidativity of blood.

We investigated whether the oxidative stress (OS) caused by skin inflammation could reflect in the blood, in a 21-year-old female student sensitized to nickel, colophony and abitole with often relapsing allergic contact dermatitis (ACD). As glutathione redox ratio was increased in the blood not only during the relapse but also in the beginning of remission phase, we prescribed natural medical preparations of d-alpha-tocopherol (in the first week 100 mg three times a day followed by 100 mg/day) and ascorbic acid (200 mg/day) for 25 days to her. After using antioxidants in the remission period, one of the principal OS markers-the glutathione redox ratio reached the normal physiological level. In this report, we showed that during acute extensive ACD OS is expressed in the blood and simultaneous supplementation of d-alpha-tocopherol and ascorbic acid might reduce systemic OS.

Nickel sulphate and epoxy resin: differences in iron status and glutathione redox ration at the time of patch testing.

The reactive patch test reaction is a useful model to characterize oxidative stress in acute allergic contact dermatitis. This model was used to study oxidative stress in the skin of individuals allergic to nickel and epoxy resin. The study included six and five patients, respectively, whose skin was simultaneously biopsied from a positive patch test site and from an apparently healthy area. The biopsies were homogenized and used for determination of iron content, unbound iron binding capacity, diene conjugate levels, and glutathione redox ratio. A positive test reaction to 5% nickel sulphate was accompanied by 2.5-fold increase in iron level as compared to apparently healthy skin (P<0.1). The percentage saturation of iron-binding capacity and the glutathione redox ratio were significantly increased (P<0.01 and P<0.05, respectively). Reactive patch test responses to 1% epoxy resin were not accompanied by clear alterations in iron status or glutathione redox ratio. Our investigation showed that apart from oxidative burst caused by accumulation of inflammatory cells, hapten properties might also influence the oxidative stress status of the skin. The high incidence of nickel allergy may be attributed, at least in part, to the influence of nickel ions on the glutathione redox ratio and iron status of the skin.