RESUMO
In the current study, we synthesized a new SiPc derivative conjugated with arginine at the axial positions, for a novel phthalocyanine-based photosensitizer for photodynamic therapy (PDT) applications in cancer cells. Axially-di-arginine substituted new silicon(IV) phthalocyanine photosensitizer (PS-5a) has been thoroughly researched for its anti-cancer properties. Various spectroscopic techniques were used to characterize this conjugate, including 1H NMR, 13C NMR, FT-IR, UV-vis, and MS spectral data. The in vitro PDT activities of the conjugate on cancer cells were tested through its cytotoxic, clonogenic, apoptotic effects on, and its capacity to induce DNA damage, and the disruption of mitochondrial membrane potential in cancer cell lines (liver; HuH-7, cervix; HeLa and breast; MCF7). Cancer cells exposed to the light illumination following uptake of the PS-5a as a photosensitizer revealed DNA breakage and collapsed mitochondrial membrane potential. The results of the present investigation demonstrate that PS-5a has a significant photo-cytotoxic effect on cancer cells. So, axially-di-arginine substituted silicon(IV) phthalocyanine could be an effective PDT agent for PDT treatment.
Assuntos
Antineoplásicos , Fotoquimioterapia , Feminino , Humanos , Fármacos Fotossensibilizantes/uso terapêutico , Fotoquimioterapia/métodos , Espectroscopia de Infravermelho com Transformada de Fourier , Células HeLaRESUMO
Mesoporous organosilica nanoparticles (PHT-PMO) have been prepared from an octa-triethoxysilylated Zn phthalocyanine precursor. These PHT-PMO nanoparticles had no dark toxicity but high phototoxicity when irradiated at 650 nm, and remarkable near-infrared phototoxicity when excited at 760 and 810 nm. The PHT-PMO were then aminated to promote electrostatic complexation with siRNA. Transfection experiments were performed upon NIR irradiation and photochemical internalization was very efficient, leading to 65% luciferase extinction in MCF-7 cancer cells expressing stable luciferase.
Assuntos
Indóis/química , Nanopartículas/química , Compostos Organometálicos/química , Fotoquimioterapia/métodos , RNA Interferente Pequeno/química , Silanos/química , Sobrevivência Celular , Cetrimônio/química , Humanos , Raios Infravermelhos , Isoindóis , Luciferases/genética , Células MCF-7 , Processos Fotoquímicos , Porosidade , RNA Interferente Pequeno/metabolismo , Eletricidade Estática , Propriedades de Superfície , Compostos de ZincoRESUMO
Photosensitizing nanogels were obtained through a surfactant-free single-step protocol by using a porphyrin-based cross-linker for stabilizing self-assembled nanosized aggregates of thermoresponsive copolymers. Nanogels with varying amounts of porphyrin retained the singlet oxygen generation ability of the porphyrin core and were also capable of inducing temperature increase upon irradiation at 635 nm. Photoinduced killing efficiency was tested against three cell lines: human breast adenocarcinoma (MDA-MB-231 and MCF7) and pancreatic adenocarcinoma (AsPC-1) cells, and a predominant photodynamic mechanism at 450 nm and a mixed photodynamic and photothermal effect at 635 nm was observed. This innovative access to photosensitizing nanogels is a proof of concept, and opens new perspectives toward the preparation of optimized nanophotosensitizers.