Ovarian activity, hormone profile, pro-inflammatory cytokines and reproductive performance of buffalo cows fed diets with different estrogenicity.

Buffalo cows play a vital role in milk and meat production; however, they are characterised by several reproductive disorders. Feeding diets with high oestrogenic activity may be a disrupting factor. This study aimed to evaluate the effects of feeding roughages with different oestrogenic activity on the reproductive performance of early postpartum buffalo cows. A total of 30 buffalo cows were equally stratified into two experimental groups and fed either Trifolium alexandrinum (Berseem clover, phytoestrogenic roughage) or corn silage (nonoestrogenic roughage) for 90 consecutive days. After 35 days from the beginning of the feeding treatments, buffalo cows in both groups were synchronized for oestrus using a double i.m. injection of 2 mL prostaglandin F2α , 11 days apart, subsequently, overt signs of oestrus were observed and recorded. Moreover, ovarian structures, numbers and sizes of follicles and corpora lutea, were ultrasonography examined at day-12 (represents Day 35 of feeding treatment), Day 0 (day of oestrus) and Day 11 after oestrous synchronization (mid-luteal phase). Pregnancy was diagnosed 35 days postinsemination. Blood serum samples were analysed for progesterone (P4 ), estradiol (E2 ), tumor necrosis factor (TNF-α), interlukein-1ß (IL-1ß) and nitric oxide (NO). The high performance liquid chromatography-analysis of roughages showed the abundance of isoflavones in Berseem clover, with about 58 times higher concentration than that in corn silage group. During the experimental period, the numbers of ovarian follicles of all size categories were higher in the Berseem clover group than that in the corn silage group. No significant difference in the numbers of corpora lutea was observed between both experimental groups, but lower (p < 0.05) diameter of corpus luteum was observed in the Berseem clover group than that in the corn silage group. The Berseem clover group had higher (p < 0.05) overall concentrations of blood serum E2 , IL-1ß and TNF-α, but lower (p < 0.05) overall concentrations of blood serum P4 than those recorded in the corn silage group. Oestrous rate, onset of oestrus time and oestrous duration were not significantly affected by the treatment. The conception rate was significantly (p < 0.05) reduced in the Berseem clover group compared with that in the corn silage group. In conclusion, feeding roughage with a high oestrogenic activity such as Berseem clover can negatively affect the conception rate of buffalo cows. This reproductive loss seems to be associated with inadequate luteal function and P4 concentration during early pregnancy.

The synergistic impact of Spirulina and selenium nanoparticles mitigates the adverse effects of heat stress on the physiology of rabbits bucks.

Heat stress has a detrimental effect on animal fertility, particularly testicular functions, including reduced sperm output and quality, which causes an economic loss in the production of rabbits. The present trial investigated the efficacy of dietary Spirulina (SP) (Arthrospira platensis), selenium nanoparticles (SeNPs), and their combination (SP-SeNPs) on semen quality, haemato-biochemical, oxidative stress, immunity, and sperm quality of heat-stressed (HS) rabbit bucks. Sixty mature bucks (APRI line) were distributed into 6 groups of ten replicates under controlled conditions. Bucks in the 1st group (control-NC) were kept under normal conditions (11-22°C; 40-45% RH% = relative humidity), while the 2nd group (control-HS) was kept under heat stress conditions (32±0.50°C; 60-66% RH %). The control groups were fed a commercial pelleted diet and the other four heat-stressed groups were fed a commercial pelleted diet with 1 g SP, 25 mg SeNPs, 1 g SP+25 mg SeNPs, and 1 g SP+50 mg SeNPs per kg diet, respectively. The dietary inclusion of SP, SeNPs, and their combinations significantly increased hemoglobin, platelets, total serum protein, high-density lipoproteins, glutathione, glutathione peroxidase, superoxide dismutase, and seminal plasma testosterone while decreased triglycerides, total cholesterol, urea, creatinine, and malondialdehyde compared with the control-HS. Red blood cells, packed cell volume, serum albumin, and testosterone significantly increased, while SeNPs, SP+SeNPs25, and SP+SeNPs50 significantly decreased low-density lipoproteins, aspartate, and alanine amino transferees. Total antioxidant capacity substantially increased in serum and seminal plasma, while seminal plasma malondialdehyde decreased in 25 or 50 mg of SeNPs+SP/kg groups. All supplements significantly improved libido, sperm livability, concentration, intact acrosome, membrane integrity, total output in fresh semen, and sperm quality in cryopreserved semen. SP-SeNPs50 had higher synergistic effect than SP-SeNPs25 on most different variables studied. In conclusion, the dietary inclusion of SP plus SeNPs50 has a synergistic effect and is considered a suitable dietary supplement for improving reproductive efficiency, health, oxidative stress, and immunity of bucks in the breeding strategy under hot climates.

Dietary supplementation of Nile tilapia (Oreochromis niloticus) with ß-glucan and/or Bacillus coagulans: Synergistic impacts on performance, immune responses, redox status and expression of some related genes.

A 14-week feeding study was conducted to assess the effects of feed supplementation with prebiotics ß-glucan (BG group) and/or probiotics Bacillus coagulans (BC group) on O. niloticus growth performance, body analysis, intestinal structure, immunological response, and antioxidant status. The fish were equally divided into six groups, as follows: the fish group fed an un-supplemented diet served as a control group; the other fish groups were fed supplemented diets with 0.1 g ß-glucan kg-1; 1 g Bacillus coagulans kg-1; 2 g B. coagulans kg-1; 0.1 g ß-glucan combined with 1 g B. coagulans kg-1; 0.1 g ß-glucan combined with 2 g B. coagulans kg-1. The findings revealed that supplementing B. coagulans and the ß-glucan mixture improved growth performance and feed efficiency parameters (RGR and SGR) more than the other groups. The fish flesh analysis revealed increased crude protein and dry matter content and lower lipid and ash levels in the BG and BC supplemented groups than in the Control group. On the other hand, ß-glucan and B. coagulans supplementation significantly boosted antioxidant activity and immunological responses in serum as determined by CAT, MDA, lysozyme, and phagocytic activity. Dietary ß-glucan and B. coagulans supplementation remarkedly enhanced anterior intestine villus histomorphometry characteristics. Furthermore, B. coagulans, alone or in combination with ß-glucan, could reduce HSP70 and IL-1ß gene expression while increasing IL-8 and GH gene expression. According to the findings, B. coagulans and/or BG increased growth performance by increasing gut health and morphology. Furthermore, ß-glucan and B. coagulans supplementation enhanced Tilapia's body composition, immunological responses, and antioxidant status.