RESUMO
A method using combined gas chromatography/mass spectrometry (GC/MS) for determination of the cocaine pyrolysis product anhydroecgonine methyl ester (AEME) in urine is described. Using this method, we found that human subjects who smoked cocaine under laboratory conditions excreted substantial amounts of AEME in their urine. Little, if any, AEME was excreted in the urine when the same subjects were administered cocaine by intravenous and intranasal routes. AEME may be a useful marker for cocaine (crack) smoking. The pharmacology of AEME is unknown. The possibility that AEME may play a role in the effects associated with cocaine smoking needs to be examined.
Assuntos
Cocaína/análogos & derivados , Cocaína/metabolismo , Fumar/urina , Administração Intranasal , Adulto , Cocaína/administração & dosagem , Cocaína/urina , Feminino , Cromatografia Gasosa-Espectrometria de Massas/métodos , Humanos , Injeções Intravenosas , MasculinoRESUMO
A method is described for extraction of the cocaine metabolite benzoylecgonine, conversion to the butyl ester derivative and gas chromatographic analysis using packed or capillary columns. Using a capillary column, cocaine and benzoylecgonine may be determined simultaneously. The extraction scheme has been designed to facilitate processing of large numbers of samples generated in pharmacokinetic studies. Structural analogues, m-toluylecgonine and m-toluylecgonine methyl ester, are used as internal standards. Concentrations as low as 10 ng/ml in 1-ml samples of plasma or urine are readily determined. Between-run coefficients of variation were 1.01% for cocaine and 4.18% for benzoylecgonine for concentrations of 75 and 350 ng/ml, respectively.
Assuntos
Cocaína/análogos & derivados , Cocaína/análise , Autoanálise , Cromatografia Gasosa , Cocaína/sangue , Cocaína/urina , Humanos , Indicadores e ReagentesRESUMO
A rapid and sensitive gas chromatographic method is described for the determination of cocaine in plasma. A close structural analogue of cocaine, m-toluylecgonine methyl ester, is used as an internal standard. The simplicity of the extraction scheme and the use of an automatic sampler makes the method convenient for large numbers of samples generated in pharmacokinetic studies.