Influence of Fabrication Technique on Adhesion and Biofilm Formation of Candida albicans to Conventional, Milled, and 3D-Printed Denture Base Resin Materials: A Comparative In Vitro Study.

The aim of this study was to evaluate the adhesion and biofilm formation of Candida albicans (C. albicans) on conventionally fabricated, milled, and 3D-printed denture base resin materials in order to determine the susceptibility of denture contamination during clinical use. Specimens were incubated with C. albicans (ATCC 10231) for 1 and 24 h. Adhesion and biofilm formation of C. albicans were assessed using the field emission scanning electron microscopy (FESEM). The XTT (2,3-(2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) assay was used for the quantification of fungal adhesion and biofilm formation. The data were analyzed using GraphPad Prism 8.02 for windows. One-way ANOVA with Tukey's post hoc testing were performed with a statistical significance level set at α = 0.05. The quantitative XTT biofilm assay revealed significant differences in the biofilm formation of C. albicans between the three groups in the 24 h incubation period. The highest proportion of biofilm formation was observed in the 3D-printed group, followed by the conventional group, while the lowest candida biofilm formation was observed in the milled group. The difference in biofilm formation among the three tested dentures was statistically significant (p < 0.001). The manufacturing technique has an influence on the surface topography and microbiological properties of the fabricated denture base resin material. Additive 3D-printing technology results in increased candida adhesion and the roughest surface topography of maxillary resin denture base as compared to conventional flask compression and CAD/CAM milling techniques. In a clinical setting, patients wearing additively manufactured maxillary complete dentures are thus more susceptible to the development of candida-associated denture stomatitis and accordingly, strict oral hygiene measures and maintenance programs should be emphasized to patients.

Relationship between sense of coherence, OHRQoL, and dental caries among nursing students in South India.

Background: Dental caries is one of the most common dental diseases that affect all population and is associated with the avoidance of care. Research has reported that sense of coherence (SOC) is related to many aspects of health including oral health. SOC determines the quality of health and might have a direct association with the development of subjective assessments of oral health. Objectives: To find the association between SOC, Oral Health-Related Quality of Life (OHRQoL) and caries status among nursing college students in southern state of India. Design: Cross-sectional design using questionnaire and assessment of caries status. Participants: Nursing students from south India. Methods: Convenience sampling method was followed and students who were present on the day of the study and consented to participate were included in the study. The total study sample consisted of 494 nursing students. SOC and OHRQoL were measured by a self-administered questionnaire; caries status was assessed using Decayed, Missing and Filled Tooth (DMFT) index. Results: Association between SOC and Oral Health Impact Profile (OHIP) and caries status and OHIP was found to be statistically significant. Correlation between dental caries and OHIP was found to be statistically significant, with R-value -0.251 shows that OHIP is negatively correlated with caries status. Conclusion: SOC as a psychosocial resource is capable of facilitating the motivation for positive oral health behaviours. These resources along with socio-economic and demographic factors can create an environment that is partially responsible for the individuals' cognitive and physical functions that can express themselves as the individuals' well-being and positive health behaviours.

Pediatric dental emergency management and parental treatment preferences during COVID-19 pandemic as compared to 2019.

Pediatric dental emergency management were temporarily suspended during the COVID-19 pandemic, which worsened urgent dental needs. This retrospective study investigated the management of pediatric emergencies during COVID-19 lockdown and the trends in parental preferences from March to July in 2019 and 2020. Pediatric dental emergencies managed during pandemic was collated, procedures were categorized (emergency, restorative, preventive, elective) and trends in parental treatment preference was compared from March-July 2019/2020. Bivariate analysis was performed using fisher-exact test and statistical significance was set at 5%. Total 1081 children were treated during COVID-19 lockdown, and 1509 procedures were performed, of which 20.8% were emergency, 42% restorative, 24.4% preventive, 12.6% elective. In 2019, 7462 children were treated; and except for emergency (10.6%), other procedures were comparable to 2020. Extractions (267) predominated in 2020 followed by sealants (195); but in 2019, pulectomy (1268), scaling (1251) were predominant. None of the residents who performed aerosol procedures got infected with COVID-19 during the lockdown. Emergency dental needs among pediatric patients were very high during the COVID-19 pandemic in South India, and there was not much change in the trend in parental treatment preference in 2019 and 2020. Further, aerosol procedures did not increase the risk of COVID-19 during the pandemic provided proper universal precautions were followed.

All-ceramic restorations: A review of the literature.

OBJECTIVES: Ceramics can simulate the visual character of the tooth substance successfully and are biocompatible materials. However, a wide range of ceramic materials and systems on the market are available for use in dentistry. Therefore, it is the aim of this article to provide an overview of dental ceramics, their classifications, methods of construction, and clinically relevant aspects that enable the reader to select the most appropriate ceramic for a particular clinical situation. MATERIAL AND METHODS: The PubMed (MEDLINE) search engine was used to gather the most recent information on dental ceramics. The search was restricted to a ten-year period (January 1, 2010-December 31, 2019) and only English-language studies. A Boolean search of the PubMed data set was implemented to combine a range of keywords: (ceramics OR all-ceramics OR dental porcelain OR polycrystalline OR porcelain fused to metal OR ceramometal OR procera OR e max OR zirconia OR In-ceram OR Inlays OR Onlays OR Overlays OR Endocrown) AND (survival rate OR success rate OR clinical outcomes OR classification) AND (humans). Studies were also obtained by manual searches and from Google Scholar. RESULTS: By using this process, 2173 articles and studies were obtained. More studies were also obtained by manual searches and from Google Scholar. The most relevant published studies were chosen and used in the current review. CONCLUSION: All-ceramic restoration use has increased in recent years. This increase has been attributed to patients' demand for good aesthetics and an improvement in the materials' mechanical and aesthetic properties as well as to required minimally invasive tooth preparation and the methods of fabrication. The success of ceramic restorations depends on several factors, such as selection of material, restoration design, occlusion, and cementation media.