Modifications of band 3 and oxidation level of membrane proteins in senescent erythrocytes.

OBJECTIVES: To study membrane proteins modifications in Senescent Red Blood Cells (SeRBC). DESIGN ANDMETHODS: SeRBC were obtained on Percoll gradients. Membrane proteins were analyzed by SDS-PAGE, band 3 by immunoblotting, and protein oxidation by measuring the carbonyl groups. RESULTS: Densitometric analysis showed no change in SeRBC while an increase in band 3 and its degradation products was found. An increase of protein oxidation level was found in SeRBC. CONCLUSIONS: These findings provide further experimental evidence about protein modifications occurring during the RBC lifespan.