Revalorization of cellulosic fiber extracted from the waste stem of Brassica oleracea var. botrytis L. (cauliflower) by characterizing for potential composite applications.

This study investigates a protocol for extracting and characterizing fibers obtained from cauliflower (Brassica oleracea var. botrytis L.) stem agricultural waste, exploring its suitability for composite applications. Brassica oleracea var. botrytis L. (BOVBL), commonly known as cauliflower, was comprehensively characterized for the first time, with its fiber extracted from plant waste stems. BOVBL fiber, subjected to microbial degradation, exhibited properties typical of natural fibers, with a density of 1.47 g/cm3 and a composition of 50.09 % cellulose, 19.7 % hemicellulose, and 22.3 % lignin. XPS analysis showed that the surface structure of the fiber consisted of carbon (64.37 %) and oxygen (22.36 %) due to cellulose. The crystalline index is calculated as 57.32 % indicating a highly organized molecular arrangement. SEM images depicted a rough surface with hexagonal and rectangular forms, enhancing resin penetration for improved composite adhesion. The thermal analysis demonstrated stability up to 324.38 °C, promising suitability for composite heat processing. The results of the single fiber test (tensile strength, E-modulus, and elongation at break) were assessed by using Weibull distribution analysis. This investigation provides suggestions for the potential applications of organic waste leftovers as a new, environmentally friendly material for fiber-reinforced polymer composites aligning with circular economy and sustainability through the utilization of agricultural waste in the future.

Evaluation of the interaction between proliferation, oxidant-antioxidant status, Wnt pathway, and apoptosis in zebrafish embryos exposed to silver nanoparticles used in textile industry.

Antimicrobial textile products are developing rapidly as an important part of functional textiles. Silver nanoparticles (AgNPs) are nanotechnology products with antimicrobial properties. However, exposure to nanoparticles in daily life is an important issue for public health, still being updated. Aim was to evaluate the effects of AgNPs on the development of zebrafish embryos focusing on Wnt pathway, proliferation, oxidant-antioxidant status, and apoptosis. The expressions of ccnd1 and gsk3ß were determined by RT-PCR, whereas ß-catenin and proliferative cell antigen (PCNA) expressions were determined immunohistochemically. Lipid peroxidation, superoxide dismutase, and glutathione-S-transferase activities were determined spectrophotometrically. Apoptosis was determined using acridine orange staining. Oxidant status, apoptosis, immunohistochemical PCNA, and ß catenin staining increased, whereas ccnd1 and antioxidant enzyme activities decreased in AgNPs-exposed embryos in a dose-dependent manner. Our results indicate the interaction of possible mechanisms that may be responsible for the toxic effects of AgNPs in zebrafish embryos.