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1.
Exp Appl Acarol ; 92(2): 241-252, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38321309

RESUMO

Tick-borne relapsing fever spirochetes of genus Borrelia thrive in enzootic cycles involving Ornithodoros spp. (Argasidae) mainly, and rodents. The isolation of these spirochetes usually involves a murine model in which ticks are fed and the spirochetes detected in blood several days later. Such an experiment also demonstrates that a given species of tick is competent in the transmission of the bacteria. Here, soft ticks Ornithodoros octodontus were collected in Northern Chile with the objective to experimentally determine its capacity to transmit a Borrelia sp. detected in a previous study. Two Guinea pigs (Cavia porcellus) were used to feed nymphs and adults of O. octodontus and the spirochetes in blood were inspected by dark-field microscopy and nested PCR. Although spirochetes were not seen in blood, DNA was detected in only one animal 11 days after the ticks were fed. Genetic sequences of Borrelia flaB, clpX, pepX, recG, rplB, and uvrA genes retrieved from DNA extraction of positive blood were employed to construct two phylogenetic analyses. On the one hand, the flaB tree showed the Borrelia sp. transmitted by O. octodontus clustering with Borrelia sp. Alcohuaz, which was previously detected in that same tick species. On the other hand, concatenated clpX-pepX-recG-rplB-uvrA demonstrated that the characterized spirochete branches together with "Candidatus Borrelia caatinga", a recently discovered species from Brazil. Based on the genetic profile presented in this study, the name "Candidatus Borrelia octodonta" is proposed for the species transmitted by O. octodontus. The fact that spirochetes were not observed in blood of guinea pigs, may reflect the occurrence of low spirochetemia, which could be explained because the susceptibility of infection varies depending on the rodent species that is used in experimental models. Although the vertebrate reservoir of "Ca. Borrelia octodonta" is still unknown, Octodon degus, a rodent species that is commonly parasitized by O. octodontus, should be a future target to elucidate this issue.


Assuntos
Argasidae , Borrelia , Besouros , Ornithodoros , Febre Recorrente , Doenças dos Roedores , Animais , Cobaias , Camundongos , Ornithodoros/genética , Febre Recorrente/veterinária , Febre Recorrente/epidemiologia , Febre Recorrente/microbiologia , Chile , Filogenia , Roedores , DNA
2.
Rev Med Chil ; 150(4): 415-423, 2022 Apr.
Artigo em Espanhol | MEDLINE | ID: mdl-36155750

RESUMO

BACKGROUND: The COVID-19 pandemic increased the incidence of neuropsychiatric diseases. Proactive models of consultation-liaison psychiatry (CLP-p) could play a key role in the prevention and management of these diseases in a general hospital. AIM: To develop a protocol for implementing screening tools for neuropsychiatric symptoms in routine clinical practice. MATERIAL AND METHODS: Elements of the Plan-Do-Study-Act (PDSA) model were used to modify the Neuropsychiatric Surveillance protocol implemented at a clinical hospital during the COVID-19 pandemic by members of the hospital's CLP team. RESULTS: A flowchart for active follow-up of neuropsychiatric symptoms during hospitalization is presented, with sequential management and referral flows, accompanied by suggestions for pre-discharge evaluation to define continuity of care actions. The COSMOS tool is also presented, designed for the detection of risk factors and actions for the prevention of neuropsychiatric diseases in general hospitals. CONCLUSIONS: The neuropsychiatric surveillance protocol facilitates early and timely interventions and establishes criteria for the continuity of post-discharge care. These changes could improve the quality of care in general hospitals and reduce the gap between mental and physical health.


Assuntos
COVID-19 , Hospitais Gerais , Assistência ao Convalescente , Humanos , Pandemias/prevenção & controle , Alta do Paciente , Encaminhamento e Consulta
3.
Rev. méd. Chile ; 150(4): 415-423, abr. 2022. ilus, tab
Artigo em Espanhol | LILACS | ID: biblio-1409820

RESUMO

BACKGROUND: The COVID-19 pandemic increased the incidence of neuropsychiatric diseases. Proactive models of consultation-liaison psychiatry (CLP-p) could play a key role in the prevention and management of these diseases in a general hospital. AIM: To develop a protocol for implementing screening tools for neuropsychiatric symptoms in routine clinical practice. MATERIAL AND METHODS: Elements of the Plan-Do-Study-Act (PDSA) model were used to modify the Neuropsychiatric Surveillance protocol implemented at a clinical hospital during the COVID-19 pandemic by members of the hospital's CLP team. RESULTS: A flowchart for active follow-up of neuropsychiatric symptoms during hospitalization is presented, with sequential management and referral flows, accompanied by suggestions for pre-discharge evaluation to define continuity of care actions. The COSMOS tool is also presented, designed for the detection of risk factors and actions for the prevention of neuropsychiatric diseases in general hospitals. CONCLUSIONS: The neuropsychiatric surveillance protocol facilitates early and timely interventions and establishes criteria for the continuity of post-discharge care. These changes could improve the quality of care in general hospitals and reduce the gap between mental and physical health.


Assuntos
Humanos , COVID-19 , Hospitais Gerais , Alta do Paciente , Encaminhamento e Consulta , Assistência ao Convalescente , Pandemias/prevenção & controle
4.
Pharmaceutics ; 13(12)2021 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-34959381

RESUMO

Chronic and non-healing wounds demand personalized and more effective therapies for treating complications and improving patient compliance. Concerning that, this work aims to develop a suitable chitosan-based thermo-responsive scaffold to provide 24 h controlled release of Dexketoprofen trometamol (DKT). Three formulation prototypes were developed using chitosan (F1), 2:1 chitosan: PVA (F2), and 1:1 chitosan:gelatin (F3). Compatibility tests were done by DSC, TG, and FT-IR. SEM was employed to examine the morphology of the surface and inner layers from the scaffolds. In vitro release studies were performed at 32 °C and 38 °C, and the profiles were later adjusted to different kinetic models for the best formulation. F3 showed the most controlled release of DKT at 32 °C for 24 h (77.75 ± 2.72%) and reduced the burst release in the initial 6 h (40.18 ± 1.00%). The formulation exhibited a lower critical solution temperature (LCST) at 34.96 °C, and due to this phase transition, an increased release was observed at 38 °C (88.52 ± 2.07% at 12 h). The release profile for this formulation fits with Hixson-Crowell and Korsmeyer-Peppas kinetic models at both temperatures. Therefore, the developed scaffold for DKT delivery performs adequate controlled release, thereby; it can potentially overcome adherence issues and complications in wound healing applications.

5.
PeerJ ; 8: e10172, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33240600

RESUMO

Jatropha curcas L. belongs to Euphorbiaceae family, and it synthesizes flavonoid and diterpene compounds that have showed antioxidant, anti-inflammatory, anticancer, antiviral, antimicrobial, antifungal and insecticide activity. Seeds of this plant accumulate phorbol esters, which are tigliane type diterpenes, reported as toxic and, depending on its concentration, toxic and non-toxic varieties has been identified. The aim of this work was to characterize the chemical profile of the extracts from seeds, leaves and callus of both varieties (toxic and non-toxic) of Jatropha curcas, to verify the presence of important compounds in dedifferentiated cells and consider the possibility of using these cultures for the massive production of metabolites. Callus induction was obtained using NAA (1.5 mg L-1) and BAP (1.5 mg L-1) after 21 d for both varieties. Thin layer chromatography analysis showed differences in compounds accumulation in callus from non-toxic variety throughout the time of culture, diterpenes showed an increase along the time, in contrast with flavonoids which decreased. Based on the results obtained through microQTOF-QII spectrometer it is suggested a higher accumulation of phorbol esters, derived from 12-deoxy-16-hydroxy-phorbol (m/z 365 [M+H]+), in callus of 38 d than those of 14 d culture, from both varieties. Unlike flavonoids accumulation, the MS chromatograms analysis allowed to suggest lower accumulation of flavonoids as the culture time progresses, in callus from both varieties. The presence of six glycosylated flavonoids is also suggested in leaf and callus extracts derived from both varieties (toxic and non-toxic), including: apigenin 6-C-α-L-arabinopyranosyl-8-C-ß -D-xylopyranoside (m/z 535 [M+H]+), apigenin 4'-O-rhamnoside (m/z 417 [M+H]+), vitexin (m/z 433 [M+H]+), vitexin 4'-O-glucoside-2″-O-rhamnoside (m/z 741 [M+H]+), vicenin-2 (m/z 595 [M+H]+), and vicenin-2,6″-O-glucoside (m/z 757 [M+H]+).

6.
Electrophoresis ; 41(20): 1776-1783, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-31267584

RESUMO

Rosmarinus officinalis L. (Lamiaceae) is an aromatic plant widely popular mainly due to its uses in traditional medicine as an anti-inflammatory, diuretic and antimicrobial, as well as in the prevention and treatment of diseases. These biological activities are mainly related to the presence of phenolic and terpenic compounds. This work reports a chemical profile analysis of extracts from leaves and calli of rosemary obtained by both pressurized liquid extraction and maceration. Chemical profiles were determined on calli extracts of 3, 6, 9, and 15 days of culture; chemical characterization and quantification of compounds was carried out using ultrahigh performance liquid chromatography-mass spectrometry. A total of 53 metabolites were identified in callus and 47 compounds in leaf extracts, of which 25 correspond to phenolic compounds, mainly flavonoids and flavones, 13 terpenes that include phenolic terpenes and one diterpenolactone, two glycosides which correspond to 6-O-caffeoyl-ß-D-fructofuranosil-(2→1)-α-D-glucopyranoside and primulaverin, an aromatic compound identified as fenantrenone and a growth regulator 12-hydroxy jasmonic acid. These results showed that undifferentiated rosemary cells accumulate the same compounds identified mainly in highly specialized tissues such as leaves. The plant cell culture supply the possibility of developing biotechnological processes to obtain compounds of commercial interest.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massas/métodos , Extratos Vegetais/química , Folhas de Planta/química , Rosmarinus/química , Cinamatos/análise , Cinamatos/química , Depsídeos/análise , Depsídeos/química , Flavonoides/análise , Flavonoides/química , Glicosídeos/análise , Glicosídeos/química , Terpenos/análise , Terpenos/química , Ácido Rosmarínico
7.
Rev. méd. Hosp. José Carrasco Arteaga ; 9(1): 28-35, MARZO 2017. Tablas, Gáficos
Artigo em Espanhol | LILACS | ID: biblio-1017336

RESUMO

INTRODUCCIÓN: La cirugía bariátrica constituye en la actualidad una alternativa terapéutica eficaz en pacientes con obesidad y comorbilidades. El objetivo fue describir los resultados obtenidos en una serie de pacientes tratados con cirugía bariátrica en el Hospital de Especialidades José Carrasco Arteaga. MÉTODO: Estudio descriptivo, longitudinal y retrospectivo realizado en los pacientes tratados con cirugía bariátrica e ingresados en el departamento de Cirugía del Hospital de Especialidades José Carrasco Arteaga durante el periodo comprendido entre 2009 y 2013. Se estudiaron las medidas antropométricas, niveles de glicemia, perfil lipídico (colesterol, triglicéridos y HDL) y los niveles de tensión arterial previos a la cirugía y 3 controles posteriores (tercer, sexto y duodécimo mes posquirúrgico) para proceder a describir los resultados y compararlos en el tiempo de seguimiento. RESULTADOS: Se estudiaron 71 pacientes con diagnóstico de obesidad mórbida (81.5 % mujeres y 18.5 % varones) con una edad media de 44 ±10 años. El peso medio prequirúrgico fue de 110.16 ±18.6 kg y el IMC de 42.6 ±46 Kg/m2. Al tercer mes, sexto mes y un año después del procedimiento quirúrgico, el IMC promedio fue de 34.54 Kg/m2, 31.64 Kg/m2 y 30.36 Kg/m2 respectivamente. El 90.8 % de los pacientes con dislipidemia, el 100 % de los diabéticos y el 98.5 % de los hipertensos normalizaron sus perfiles correspondientes tras 12 meses. El 7.04 % de los pacientes presentaron complicaciones posquirúrgicas y la mortalidad alcanzó un 5.6 %. CONCLUSIÓN: La cirugía bariátrica constituye un procedimiento útil en la actualidad para el control de la obesidad en sus distintos grados y la reducción de los efectos de las comorbilidades asociadas a ésta como la hipertensión, diabetes y dislipidemia.(au)


BACKGROUND: Bariatric surgery is consideredan effective treatment option in patients with obesity and comorbidities. The aim was to describe the results of bariatric surgery in patients from José Carrasco Arteaga Specialities Hospital. METHODS: A retrospective longitudinal descriptive study was performed in patients treated with bariatric surgery and admitted to Surgery department at José Carrasco Arteaga Specialities Hospital from 2009 to 2013. Anthropometric measures, glucose blood levels, lipid panel (cholesterol, triglycerides and HDL) and blood pressure measures were registered before the surgery and three, six and twelve months after it to describe the results and compare them through follow-up. RESULTS: 71 patients diagnosed with morbid obesity (81.5 % male and 18.5 % female) were studied; their mean age was 45 ±10 years. Average preoperative weight was 110.16 ±18.6 kg and BMI was 42.6 ±4.6 kg/m2. BMI decreased to 34.54kg/m2, 31.64kg/m2 and 30.36kg/m2 after three, six and twelve months respectively. 90.8 % of patients with dyslipidemia, 100 % of diabetics and 98.5 % of hypertensive patients had normal values of their profiles after 12 months. Postoperative complications represented 7.04 % and mortality rate reached 5.6 %. CONCLUSION: Bariatric surgery is a useful procedure to control obesity and the effects associated to its comorbidities such as arterial hypertension, diabetes and dyslipidemia.(au)


Assuntos
Humanos , Masculino , Feminino , Comorbidade , Cirurgia Bariátrica , Obesidade/terapia , Estado Nutricional
8.
Plant Biotechnol (Tokyo) ; 34(4): 187-192, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-31275026

RESUMO

Tagetes erecta is an asteraceous plant of industrial, ornamental and medicinal importance; its inflorescences have been used as a pigment source for food coloring, mainly for poultry skin and eggs. Nevertheless, there are few reports on plant regeneration or micropropagation, because unsuccesfull results in the plant's reaction to the growth regulators, developing embryogenesis on Tagetes erecta. In this study, somatic embryogenesis was induced and plantlets of Tagetes erecta were regenerated. For induction of globular structures MS medium supplemented with 2,4-D (4.5 µM) and BAP (8.8 µM) was used; globular structures were transferred to MS medium with 45 g l-1 sucrose until the embryos maturation. Transmission electron microscopy showed characteristic subcellular structures of embryogenic callus. Somatic embryos were transferred to MS medium without plant growth regulators and whole plantlets were obtained. In vitro plants were successfully transplanted into a mixture of peat moss and vermiculite (1 : 1 v/v) under greenhouse conditions. In this study, somatic embryogenesis and plant regeneration system from foliar explants were established, an important requirement for performing genetic transformation events on Tagetes erecta.

9.
Nutr Hosp ; 33(4): 380, 2016 Jul 19.
Artigo em Espanhol | MEDLINE | ID: mdl-27571658

RESUMO

Introducción: el entrenamiento de intervalos de alta intensidad (HIIT) y el consumo de ácidos grasos omega-3 (O3) ha demostrado cada uno por separado aumentar la capacidad aeróbica, metabolismo oxidativo y función cardiovascular.Objetivo: examinar el efecto combinado de HIIT más suplementación de O3 en el rendimiento físico, presión arterial y composición corporal en jóvenes sedentarios.Método: 28 jóvenes sedentarios con sobrepeso (Edad=22 ± 4 años; IMC=25.8 ± 2.4 kg·m-2) fueron distribuidos aleatoriamente en cuatro grupos: grupo O3/HIIT (n=7) realizó un protocolo de HIIT, tres veces por semana durante seis semanas y consumió 2 g·día-1 de O3; grupo HIIT (n=7) realizó solo el HIIT; grupo O3 (n=7) solo consumió O3; y grupo CONTROL (n=7) que no realizó ninguna intervención. Consumo de oxígeno peak (VO2peak), velocidad máxima (Vmax), presión arterial sistólica y diastólica (PAS y PAD), y porcentaje de grasa fueron medidos antes y después de la intervención.Resultados: el consumo de oxígeno peak aumentó más en el grupo O3/HIIT (+10.9%) en comparación con HIIT, O3 y CONTROL. Velocidad máxima aumentó en O3/HIIT (+7.1%) y HIIT (+11.9%). La presión arterial sistólica disminuyó más en O3 (-6.8%) en comparación con O3/HIIT, HIIT y CONTROL. Por último, O3/HIIT (-19.2%), HIIT (-20.2%), y O3 (-15.2%) presentaron mayores disminuciones del porcentaje de masa grasa en relación al CONTROL.Conclusión: nuestros resultados sugieren un efecto potenciador de la capacidad aeróbica máxima producto de la combinación de HIIT y suplementación de O3. Además, se observó una disminución de masa grasa en todos los grupos intervenidos.


Assuntos
Pressão Sanguínea , Composição Corporal , Exercício Físico , Ácidos Graxos Ômega-3/uso terapêutico , Sobrepeso/terapia , Educação Física e Treinamento/métodos , Adulto , Limiar Anaeróbio , Índice de Massa Corporal , Suplementos Nutricionais , Feminino , Humanos , Masculino , Sobrepeso/tratamento farmacológico , Comportamento Sedentário , Adulto Jovem
10.
Plant Foods Hum Nutr ; 71(1): 109-14, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26646703

RESUMO

Roselle is a plant that accumulates anthocyanins significantly, hence its importance as food coloring and as a source of antioxidant compounds for human health. This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship. Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside). The antioxidant capacity was evaluated by ABTS and FRAP assays. Tristimulus colorimetry showed to be a useful technique to determine the color-composition relationship, leading to equations that allowed to predict anthocyanin content of roselle (R > 0.84). Also, a stepwise linear discriminant analysis (SLDA) was developed in order to classify roselle cultivars. The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.


Assuntos
Antocianinas/análise , Antioxidantes/análise , Hibiscus/química , Fenóis/análise , Extratos Vegetais/isolamento & purificação , Antocianinas/metabolismo , Antioxidantes/metabolismo , Bebidas/análise , Cor , Dissacarídeos/análise , Dissacarídeos/metabolismo , México , Análise Multivariada , Fenóis/metabolismo , Pigmentação , Extratos Vegetais/química
11.
Rev. fac. cienc. méd. (Impr.) ; 12(2): 41-49, jul.-dic. 2015. ilus
Artigo em Espanhol | LILACS | ID: biblio-903

RESUMO

El ácido hialurónico es un glicosaminoglicano presente en todos los tejidos del cuerpo, matrizextracelular y revistiendo vasos sanguíneos. El ácido hialurónico es igual en todo el cuerpo y debido a estoes biocompatible al ser aplicado en los seres humanos, tiene diversos usos que lo hacen actualmente objeto de investigaciones. En personas con cáncer de pulmón reduce la propagación de células tumorales e incrementa la regeneración de tejido sano. Tambiénse utiliza en tratamientos para la artritis, ya que resulta beneficioso al regenerar cartílago de las articulaciones y por ende un alivio en personas con dichos problemas. El ácido hialurónico ha tenido aceptación en su utilización para el tratamiento estético, teniendo resultados considerables y con duración semipermanente. Otro uso destacable es su aplicación para el ojo seco, que por su presentación en gotas brinda al paciente una hidratación, permitiendoasí curar este mal. Por los múltiples usos del ácido hialurónico es importante estudiar más sobre él, proponiendo nuevas alternativas en diferentes áreas de salud. La metodología usada en la presente revisión, fue la búsqueda sistemática de artículos actualizadosen revistas médicas nacionales e internacionales; posteriormente se filtró la información obtenida basándose en el objetivo general de la revisión: Descripción de las aplicaciones clínicas del ácido hialurònico, en diferentes áreas de la medicina..(AU).


Assuntos
Humanos , Bases de Dados Bibliográficas/estatística & dados numéricos , Ácido Hialurônico/farmacocinética , Polissacarídeos , Regeneração/genética
12.
Rev. biol. trop ; 63(supl.2): 49-58, Apr.-Jun. 2015. tab, graf
Artigo em Espanhol | LILACS, SaludCR | ID: biblio-958158

RESUMO

Resumen Astropyga pulvinata es un diademátido que posee cinco pares de series de puntos iridiscentes presentes en los interambulacros. A partir de la observación 20 individuos in vivo a través de un estereoscopio y el análisis con un Microscopio Electrónico de Barrido se identificaron los pedicelarios, las espículas y las rosetas calcáreas de los pies ambulacrales. Los pedicelarios presentes en A. pulvinata son trivalvados y de tres tipos: claviformes (oficéfalos) presentes en ambulacro; tridentados distribuidos en el peristoma; y trifoliados presentes en toda la testa excepto en los interambulacros. Los pedicelarios claviformes son los únicos que no están unidos por un pedúnculo calcáreo de tipo diadematoide. Se obtuvieron diez tipos de espículas de diversos tejidos blandos: uno del intestino; tres de la membrana anal y seis del peristoma, dos de las cuales no han sido descritas hasta el momento. Uno de estos nuevos tipos de espículas reportados posee forma de basto y otro de media luna. De las observaciones in vivo se encontraron puntos iridiscentes más pequeños presentes en el ambitus constituidos por aproximadamente ocho células.


Abstract Astropyga pulvinata is a sea urchin of the family Diadematidae that have five pairs of series of blue iridescent spots on interambulacral zones, from in vivo observation of 20 individuals under stereo-microscope and Scanning Electron Microscope analysis identification, the pedicellariae, spicules, and calcareous rosettes. The pedicellariae of A. pulvinata have three valves and are of three types: claviform, present on ambulacres; tridentates present on peristome and triphyllous present all over the test except in interambulacral areas. So ophicephalous pedicellariae are not bounded with a calcareous stalk. Ten types of spicules were observed from different soft tissues: one type from the intestine; three from the anal membrane; and six from the peristome, two of them constitute new diagnostic characters. One of them having a bat-shaped form and the other one with a waning crescent shape. In vivo observations showed that the blue iridescent spots were present on interambulacral areas as group of 35 cells bounded, and that other smaller spots were present on the ambitus, composed by eight cells. Rev. Biol. Trop. 63 (Suppl. 2): 49-58. Epub 2015 June 01.


Assuntos
Animais , Ouriços-do-Mar/anatomia & histologia , Equinodermos/anatomia & histologia , Classificação , Sistemas de Identificação Animal
13.
Electron. j. biotechnol ; 17(3): 107-113, May 2014. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-719099

RESUMO

Background The properties of natural pigments, such as antioxidants, functional, medical, and nutraceutical, have demonstrated the advantages of these natural compounds over synthetic ones. Some products are accepted only when they are pigmented with natural, food-quality colorants: for example poultry products (manly marigold flower extracts). Carotenoids such as β-carotene, β-criptoxanthin and lutein are very attractive as natural food colorants due to their antioxidant and pro-vitamin activities which provide additional value to the target products. Marigold (Tagetes erecta) is an Asteraceous ornamental plant native to Mexico, and it is also important as a carotenoid source for industrial and medicinal purposes but nowadays its production is destined mainly for ornamental purposes. Results Friable callus of T. erecta yellow flower (YF) and white flower (WF) varieties was induced from leaf explants on Murashige and Skoog (MS) medium supplemented with 9.0 µM 4-dichlorophenoxyacetic acid (2,4-D) and 8.8 µM benzyladenine (BA). Calluses developed from both varieties were different in pigmentation. Extract characterization from callus cultures was carried out by high-performance liquid chromatography (HPLC). This analytical process detected several carotenoids; the main pigments in extracts from YF callus were lutein and zeaxanthin, whereas in the extracts of the WF callus the main pigments were lutein, zeaxanthin, β-cryptoxanthin and β-carotene. Callus cultures of T. erecta accumulated pigments even after several rounds of subculture. Conclusions WF callus appeared to be a suitable candidate as a source of different carotenoids, and tested varieties could represent an alternative for further studies about in vitro pigment production.


Assuntos
Carotenoides/biossíntese , Tagetes/crescimento & desenvolvimento , Tagetes/metabolismo , Ácido 2,4-Diclorofenoxiacético , Reguladores de Crescimento de Plantas , Luteína , Pigmentação , Cromatografia Líquida de Alta Pressão , Germinação , Técnicas de Cultura
14.
ScientificWorldJournal ; 2012: 940293, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22645473

RESUMO

The morphostructure of grain rice Morelos A-98 was characterized in five stages of physiological maturation, in order to generate morphometric information during the filling process. Micrographic images from optical and scanning electron microscopy coupled to a digital capture system were used. Images were digitally processed to measure different descriptors such as shape, fractal dimension, and surface texture. Results showed that, two weeks after anthesis, an accelerated grain filling was observed, particularly on those grains positioned in the distal panicle zone, compared to those located in the base of this one. As deposition of assimilates in the grain increased, the area and perimeter of the transversal cut of the grains also increased (P ≤ 0.05); meanwhile, the rounded shape factor tended to increase as well (P ≤ 0.05), while the elliptic shape factor decreased. As the dehydrated endosperm passed from "milky" to "doughy" stages, values of fractal dimension area and endosperm perimeter as well as surface texture values showed that grain borders tended to become smoother and that there was a greater structured endosperm area (P ≤ 0.05).


Assuntos
Oryza/genética , Oryza/fisiologia , Grão Comestível , Endosperma/fisiologia , Fractais , Variação Genética , México , Microscopia Eletrônica de Varredura/métodos , Modelos Estatísticos , Óptica e Fotônica , Sementes , Propriedades de Superfície , Temperatura
15.
Methods Mol Biol ; 589: 213-21, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20099104

RESUMO

Marigold (Tagetes erecta) is an Asteraceous plant of industrial, ornamental and medicinal importance. Tagetes erecta species, popularly known as marigold, is grown as ornamental plant and is adapted to several agro climates. Inflorescences have been utilized as pigment source for food coloring, mainly of poultry skin and eggs. Lutein is the main pigment in marigold flowers. Some carotenoids are well known as provitamin A compounds. There are many reports on carotenoids and their effect on the prevention of certain ocular diseases, ischemic heart disease, strokes, photoprotection, immune response, aging and cancer. Marigold flowers are certainly a good source of carotenoids; they show very different pigmentation levels. This chapter describes the establishment of techniques for plant regeneration, gene expression, pigment extraction and pigment deposition in specific structures of marigold.


Assuntos
Carotenoides/genética , Técnicas de Cultura , Flores/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas , Técnicas Genéticas , Regeneração/genética , Tagetes/genética , Aclimatação , Carotenoides/isolamento & purificação , Carotenoides/metabolismo , Proliferação de Células , Flores/metabolismo , Germinação , Organogênese/genética , Folhas de Planta/crescimento & desenvolvimento , Plântula/crescimento & desenvolvimento , Sementes/crescimento & desenvolvimento , Tagetes/embriologia , Tagetes/crescimento & desenvolvimento , Tagetes/metabolismo , Fatores de Tempo , Transformação Genética
16.
Parasitol. latinoam ; 57(1/2): 75-78, ene.-jun. 2002. ilus
Artigo em Espanhol | LILACS | ID: lil-317523

RESUMO

Loxosceles laeta es una araña de importancia en Chile por su alta morbiletalidad en humanos. La gran mayoría de los pacientes con mordedura por esta araña consultan en servicios de atención primaria y de urgencia. Es relevante conocer su morfología para identificarla en forma precoz y tener una conducta médica adecuada a seguir. Se ilustra su morfología con fotos de microscopía de barrido destacando sus aspectos más fundamentales entre ellos sus seis ojos dispuestos en forma triangular


Assuntos
Animais , Aranhas/ultraestrutura , Picada de Aranha , Aranhas/anatomia & histologia , Aranhas/crescimento & desenvolvimento , Ecossistema , Microscopia de Força Atômica
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