Endogenous Bornavirus-like Elements in Bats: Evolutionary Insights from the Conserved Riboviral L-Gene in Microbats and Its Antisense Transcription in Myotis daubentonii.

Bats are ecologically diverse vertebrates characterized by their ability to host a wide range of viruses without apparent illness and the presence of numerous endogenous viral elements (EVEs). EVEs are well preserved, expressed, and may affect host biology and immunity, but their role in bat immune system evolution remains unclear. Among EVEs, endogenous bornavirus-like elements (EBLs) are bornavirus sequences integrated into animal genomes. Here, we identified a novel EBL in the microbat Myotis daubentonii, EBLL-Cultervirus.10-MyoDau (short name is CV.10-MyoDau) that shows protein-level conservation with the L-protein of a Cultervirus (Wuhan sharpbelly bornavirus). Surprisingly, we discovered a transcript on the antisense strand comprising three exons, which we named AMCR-MyoDau. The active transcription in Myotis daubentonii tissues of AMCR-MyoDau, confirmed by RNA-Seq analysis and RT-PCR, highlights its potential role during viral infections. Using comparative genomics comprising 63 bat genomes, we demonstrate nucleotide-level conservation of CV.10-MyoDau and AMCR-MyoDau across various bat species and its detection in 22 Yangochiropera and 12 Yinpterochiroptera species. To the best of our knowledge, this marks the first occurrence of a conserved EVE shared among diverse bat species, which is accompanied by a conserved antisense transcript. This highlights the need for future research to explore the role of EVEs in shaping the evolution of bat immunity.

Probing the in situ Elastic Nonlinearity of Rocks with Earth Tides and Seismic Noise.

Heterogeneous materials such as rocks, concrete, and granular materials exhibit a strong elastic nonlinearity. The sensitivity of the elastic nonlinearity to the applied stress and pore pressure in principle allows the use of seismic waves for remote observations of stress or pore pressure changes. Yet the nonlinearity of rocks is difficult to quantify in situ as active deformation tests are not possible in the field. We investigate the elastic nonlinearity in a fully natural experiment using the ambient seismic noise of a single seismic station to sense changes of the seismic velocity in the subsurface reaching 0.026% in response to the minute deformation caused by various constituents of the tidal forces exerted by the Sun and Moon.